KEGG   ENZYME: 4.4.1.1Help
Entry
EC 4.4.1.1                  Enzyme                                 
Name
cystathionine gamma-lyase;
homoserine deaminase;
homoserine dehydratase;
cystine desulfhydrase;
cysteine desulfhydrase;
gamma-cystathionase;
cystathionase;
homoserine deaminase-cystathionase;
gamma-CTL;
cystalysin;
cysteine lyase;
L-cystathionine cysteine-lyase (deaminating)
Class
Lyases;
Carbon-sulfur lyases;
Carbon-sulfur lyases (only sub-subclass identified to date)
BRITE hierarchy
Sysname
L-cystathionine cysteine-lyase (deaminating; 2-oxobutanoate-forming)
Reaction(IUBMB)
L-cystathionine + H2O = L-cysteine + NH3 + 2-oxobutanoate (overall reaction) [RN:R01001];
(1a) L-cystathionine = L-cysteine + 2-ammoniobut-2-enoate [RN:R08632];
(1b) 2-ammoniobut-2-enoate + H2O = 2-oxobutanoate + NH3 (spontaneous) [RN:R08637]
Reaction(KEGG)
Substrate
L-cystathionine [CPD:C02291];
H2O [CPD:C00001];
2-ammoniobut-2-enoate [CPD:C17234]
Product
L-cysteine [CPD:C00097];
NH3 [CPD:C00014];
2-oxobutanoate [CPD:C00109];
2-ammoniobut-2-enoate [CPD:C17234]
Comment
A multifunctional pyridoxal-phosphate protein. Also catalyses elimination reactions of L-homoserine to form H2O, NH3 and 2-oxobutanoate, of L-cystine, producing thiocysteine, pyruvate and NH3, and of L-cysteine producing pyruvate, NH3 and H2S.
Pathway
Glycine, serine and threonine metabolism
Cysteine and methionine metabolism
Selenocompound metabolism
Metabolic pathways
Orthology
K01758  
cystathionine gamma-lyase
Genes
HSA: 
1491(CTH)
PTR: 
456939(CTH)
PPS: 
GGO: 
PON: 
MCC: 
MMU: 
107869(Cth)
RNO: 
24962(Cth)
CFA: 
479991(CTH)
AML: 
FCA: 
101083282(CTH)
BTA: 
539159(CTH)
SSC: 
733654(CTH)
ECB: 
100053614
MDO: 
100029728
SHR: 
100932012(CTH)
OAA: 
100073790
GGA: 
424716(CTH)
MGP: 
100541672
TGU: 
100232398
ACS: 
100555553
XLA: 
494673(cth)
XTR: 
394634(cth)
DRE: 
322055(cth)
TRU: 
101064271
OLA: 
101159091
BFO: 
BRAFLDRAFT_119287
CIN: 
100184574
SPU: 
762952
DME: 
Dmel_CG5345(Eip55E)
DAN: 
Dana_GF11978
DER: 
Dere_GG21913
DSE: 
Dsec_GM21904
DSI: 
Dsim_GD11400
DWI: 
Dwil_GK19674
DYA: 
Dyak_GE11987
DGR: 
Dgri_GH13557
DMO: 
Dmoj_GI18710
DVI: 
Dvir_GJ15425
AGA: 
AgaP_AGAP011172
AAG: 
AaeL_AAEL004059
CQU: 
CpipJ_CPIJ003148
AME: 
412460
NVI: 
100116486(NV13252)
TCA: 
661175
API: 
100159560
PHU: 
Phum_PHUM257250
CEL: 
CELE_C12C8.2(cbl-1) CELE_F22B8.6(cth-1) CELE_ZK1127.10(cth-2)
CBR: 
CBG24746
BMY: 
Bm1_05320
TSP: 
Tsp_06337
SMM: 
Smp_083650
NVE: 
NEMVE_v1g184363
HMG: 
100214967
TAD: 
TRIADDRAFT_36312
CME: 
CMT389C
SCE: 
YAL012W(CYS3)
AGO: 
AGOS_AEL341W
ERC: 
Ecym_2629
KLA: 
KLLA0F07909g
LTH: 
KLTH0D02728g
PPA: 
PAS_chr1-4_0489
VPO: 
Kpol_1044p10
ZRO: 
ZYRO0D05742g
CGR: 
CAGL0H06369g
NCS: 
NCAS_0F01520(NCAS0F01520)
NDI: 
NDAI_0H02400(NDAI0H02400)
TPF: 
TPHA_0L00570(TPHA0L00570)
TBL: 
TBLA_0E02240(TBLA0E02240)
TDL: 
TDEL_0H03530(TDEL0H03530)
KAF: 
KAFR_0K00760(KAFR0K00760)
DHA: 
DEHA2C15686g
PIC: 
PICST_77247(CYS3)
PGU: 
PGUG_01002
LEL: 
LELG_03059
CAL: 
CaO19.13760(CYS3) CaO19.6402(CYS3)
CTP: 
CTRG_03091
CDU: 
CD36_33930
COT: 
CORT_0G02290
YLI: 
YALI0F05874g
CLU: 
CLUG_02784
NCR: 
NCU09230
SMP: 
SMAC_04891
PAN: 
PODANSg5778
MGR: 
MGG_10380
FGR: 
FG01177.1
NHE: 
NECHADRAFT_103090
SSL: 
SS1G_01702
BFU: 
BC1G_08021
ANI: 
AN1446.2
NFI: 
NFIA_097070
AFM: 
AFUA_8G04340
AOR: 
AOR_1_106034(AO090103000051)
ANG: 
ANI_1_1172144(An16g08720)
AFV: 
AFLA_011740
ACT: 
ACLA_057690
PCS: 
Pc21g05430
CIM: 
CIMG_02214
CPW: 
CPC735_038820
URE: 
UREG_01504
ABE: 
ARB_06473
PNO: 
SNOG_15232
PTE: 
PTT_17380
TML: 
GSTUM_00010528001
CNE: 
CNN01730
CNB: 
CNBN1680
PPL: 
POSPLDRAFT_88894
LBC: 
LACBIDRAFT_185287 LACBIDRAFT_253660
CCI: 
CC1G_02572
SCM: 
SCHCODRAFT_77605
UMA: 
UM00962.1
MBR: 
MONBRDRAFT_12618
NGR: 
NAEGRDRAFT_55676
DDI: 
DDB_G0269122(cysA)
DPP: 
DICPUDRAFT_147848
TET: 
TTHERM_00052400
PTM: 
GSPATT00029327001
PTI: 
PHATRDRAFT_15917
PIF: 
PITG_08445
PLU: 
plu0523
PAY: 
PAU_00517
ENT: 
Ent638_1769
ENC: 
ECL_02361
ENO: 
ECENHK_09435
EEC: 
EcWSU1_01869(metC)
ENL: 
A3UG_09445
EAS: 
Entas_1788
EAE: 
EAE_18840
EAR: 
ST548_p6943
KPM: 
KPHS_45370
KPE: 
KPK_0708
KVA: 
Kvar_0681
KOX: 
KOX_02800
SPE: 
Spro_1626
SRR: 
SerAS9_1599
SRS: 
SerAS12_1599
SRA: 
SerAS13_1600
DDC: 
Dd586_1870
XCC: 
XCC0598(metB)
XCB: 
XC_3635
XCA: 
xccb100_3752
XCV: 
XCV3725
XAC: 
XAC3602(metB)
XAX: 
XACM_3502
XOO: 
XOO0778(metB)
XOM: 
XOO_0708
XOP: 
PXO_03157(metB)
XAL: 
XALc_2712
SML: 
Smlt0617(CYS1)
SMT: 
Smal_0489
PSU: 
Psesu_2462
FAU: 
Fraau_2839
PAE: 
PA0400
PAU: 
PA14_05230
PAP: 
PSPA7_0500
PAG: 
PLES_03981
PDK: 
PADK2_02015
PPU: 
PP_4594
PPF: 
Pput_1294
PPG: 
PputGB1_4124
PPW: 
PputW619_3903
PFO: 
Pfl01_3249
KKO: 
Kkor_1765
NGO: 
NGO0386
CVI: 
CV_3394 CV_4049(metB)
POL: 
Bpro_2831
CFU: 
CFU_1538(metB)
DSU: 
Dsui_2042
BBA: 
Bd3795(metB)
ADE: 
Adeh_3707
ACP: 
A2cp1_3847
AFW: 
Anae109_3845
ANK: 
AnaeK_3764
MXA: 
MXAN_2035
MFU: 
LILAB_17940
CCX: 
COCOR_06056(metB1)
HOH: 
Hoch_6707
MLO: 
mll4503 mlr1566
RPA: 
RPA0371(metB) RPA2357
CAK: 
Caul_4904
PZU: 
PHZ_c0072
BSB: 
Bresu_1607
SIL: 
SPO3344
HNE: 
HNE_1212
RRU: 
Rru_A0786
RCE: 
RC1_1986
BSU: 
BSU27250(yrhB)
BSR: 
I33_2767(yrhB)
BSL: 
A7A1_0367
BSH: 
BSU6051_27250(mccB)
BSY: 
I653_12940
BSS: 
BSUW23_13180(mccB)
BST: 
GYO_2965(yrhB)
BSO: 
BSNT_03957(yrhB)
BSN: 
BSn5_04410
BSQ: 
B657_27250(mccB)
BSUB: 
BEST7613_4225(yrhB)
BSX: 
C663_2562(mccB)
BLI: 
BL02018(yrhB)
BLD: 
BLi02853(yrhB)
BAO: 
BAMF_2533(mccB)
BAY: 
RBAM_024350(yrhB)
BAQ: 
BACAU_2449(yrhB)
BYA: 
BANAU_2593(yrhB)
BAMP: 
B938_12585
BAML: 
BAM5036_2375(mccB)
BAZ: 
BAMTA208_13370(mccB)
BQL: 
LL3_02811(mccB)
BXH: 
BAXH7_02735(yrhB)
BAMI: 
KSO_007200
BAE: 
BATR1942_11340
BAN: 
BA_4600(metC-3)
BAR: 
GBAA_4600(metC-3)
BAT: 
BAS4268
BAH: 
BAMEG_4637
BAI: 
BAA_4620
BAX: 
H9401_4389
BAL: 
BACI_c43580(metC2)
BCE: 
BC4366
BCA: 
BCE_4454(metC)
BCZ: 
BCZK4116(metC)
BCR: 
BCAH187_A4505
BCB: 
BCB4264_A4491
BCU: 
BCAH820_4452
BCG: 
BCG9842_B0745
BCQ: 
BCQ_4156(metC)
BCX: 
BCA_4484
BNC: 
BCN_4282
BCF: 
bcf_21745
BCER: 
BCK_13335
BCY: 
Bcer98_3084
BTK: 
BT9727_4105(metC)
BTL: 
BALH_3957(metC)
BTB: 
BMB171_C4033
BTT: 
HD73_4680
BTC: 
CT43_CH4389
BTF: 
YBT020_21535
BTM: 
MC28_3663
BTG: 
BTB_c45120(mccB)
BTI: 
BTG_27215
BTN: 
BTF1_20465
BTHT: 
H175_ch4458
BWE: 
BcerKBAB4_4219
BCL: 
ABC1945
BPU: 
BPUM_2360(yrhB)
BMQ: 
BMQ_4594(mccB)
BMD: 
BMD_4580(mccB)
BCK: 
BCO26_2430
BAG: 
Bcoa_1961
BJS: 
MY9_2706
BACI: 
B1NLA3E_16320
OIH: 
OB1109(metC)
GKA: 
GK2540
GTN: 
GTNG_2473
GTH: 
Geoth_1097
GTE: 
GTCCBUS3UF5_28630
GWC: 
GWCH70_2474
GYC: 
GYMC61_0979
GYA: 
GYMC52_2573
GCT: 
GC56T3_0952
GMC: 
GY4MC1_1027
GGH: 
GHH_c26140(mcc)
AFL: 
Aflv_0761
SAU: 
SA0419(metB)
SAV: 
SAV0460(yrhB)
SAW: 
SAHV_0458(yrhB)
SAH: 
SaurJH1_0495
SAJ: 
SaurJH9_0482
SAM: 
MW0415(metB)
SAS: 
SAS0418
SAR: 
SAR0460
SAC: 
SACOL0503
SAA: 
SAUSA300_0434(metB)
SAO: 
SAOUHSC_00422
SAE: 
NWMN_0425(metB)
SAD: 
SAAV_0403
SUU: 
M013TW_0425
SUV: 
SAVC_01890
SUH: 
SAMSHR1132_04050
SUE: 
SAOV_0478
SUJ: 
SAA6159_00410(metB)
SUK: 
SAA6008_00464(metB)
SUC: 
ECTR2_394
SUT: 
SAT0131_00500
SUQ: 
HMPREF0772_10062(metC)
SUZ: 
MS7_0434(mccB)
SUD: 
ST398NM01_0526
SUX: 
SAEMRSA15_03860
SUW: 
SATW20_05290
SUG: 
SAPIG0526
SUF: 
SARLGA251_03940
SAB: 
SAB0410
SUY: 
SA2981_0436(metB)
SAUB: 
C248_0510
SAUM: 
BN843_4430
SEP: 
SE2323
SER: 
SERP0095
SHA: 
SH2548(metB)
SSP: 
SSP0253
SCA: 
Sca_0087(metC)
SLG: 
SLGD_02456
SLN: 
SLUG_23510
SSD: 
SPSINT_0116
SDT: 
SPSE_2355(metB2)
SWA: 
A284_11085
HHD: 
HBHAL_2023(mccB)
BBE: 
BBR47_47850(mccB)
PJD: 
Pjdr2_5104
PPY: 
PPE_02107
PPM: 
PPSC2_c2348
PPO: 
PPM_2116(yrhB)
PTA: 
HPL003_19390
MPA: 
MAP3053c
MAV: 
MAV_3867
MSM: 
MSMEG_2394
MVA: 
Mvan_2142
MGI: 
Mflv_4220
MSP: 
Mspyr1_35630
MAB: 
MAB_3287c
MMC: 
Mmcs_1928
MKM: 
Mkms_1974
MJL: 
Mjls_1908
RHA: 
RHA1_ro06506
RER: 
RER_24150
ROP: 
ROP_65570
SRT: 
Srot_2032
SCO: 
SCO3920(cysA)
SMA: 
SAV_4273(cysA1)
SGR: 
SGR_3660
SCB: 
SCAB_46251(cysA)
MLU: 
Mlut_22690
KFL: 
Kfla_4026
ACE: 
Acel_1425
GOB: 
Gobs_4046
SEN: 
SACE_6136
PDX: 
Psed_1840
AMI: 
Amir_5997
STP: 
Strop_1267
SAQ: 
Sare_1158
MAU: 
Micau_1338
MIL: 
ML5_1584
VMA: 
VAB18032_11365
BLO: 
BL1559
CWO: 
Cwoe_1684
ABA: 
Acid345_0668
ACM: 
AciX9_2857
PHE: 
Phep_2908
SLI: 
Slin_5637
FJO: 
Fjoh_0710
GAU: 
GAU_0234 GAU_2431(metC)
RBA: 
RB6443(metB)
AVA: 
Ava_4155 Ava_4213
TPT: 
Tpet_1501
TLE: 
Tlet_0460
MBN: 
Mboo_1991
MPL: 
Mpal_1156
MEZ: 
Mtc_1605
HAL: 
VNG0796G(cgs)
HSL: 
OE2173F(metB2)
HTU: 
Htur_4640
NMG: 
Nmag_1099
HVO: 
HVO_2946(cgs)
HXA: 
Halxa_0671
TAC: 
Ta0080
TVO: 
TVN0174
PYN: 
PNA2_1581
PYA: 
PYCH_09080
TBA: 
TERMP_01385
APE: 
APE_1226(metC)
 » show all
Taxonomy
Reference
1
  Authors
Braunstein, A.E. and Azarkh, R.M.
  Title
[Participation of vitamin B6 in enzymic formation of hydrogen sulfide from L-cysteine.].
  Journal
Dokl. Akad. Nauk. S.S.S.R. 71 (1950) 93-96.
Reference
2
  Authors
Braunstein, A.E. and Azarkh, R.M.
  Title
[Phosphopyridoxal in aerobic deamination of homoserine and serine.].
  Journal
Dokl. Akad. Nauk. S.S.S.R. 85 (1952) 385-388.
Reference
3  [PMID:14209951]
  Authors
FLAVIN M, SEGAL A.
  Title
PURIFICATION AND PROPERTIES OF THE CYSTATHIONINE GAMMA-CLEAVAGE ENZYME OF NEUROSPORA.
  Journal
J. Biol. Chem. 239 (1964) 2220-7.
  Organism
Neurospora sp.
Reference
4  [PMID:13641250]
  Authors
MATSUO Y, GREENBERG DM.
  Title
A crystalline enzyme that cleaves homoserine and cystathionine. III. Coenzyme resolution, activators, and inhibitors.
  Journal
J. Biol. Chem. 234 (1959) 507-15.
  Organism
Rattus norvegicus [GN:rno]
Reference
5  [PMID:13641251]
  Authors
MATSUO Y, GREENBERG DM.
  Title
A crystalline enzyme that cleaves homoserine and cystathionine. IV. Mechanism of action, reversibility, and substrate specificity.
  Journal
J. Biol. Chem. 234 (1959) 516-9.
  Organism
Rattus norvegicus [GN:rno]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
9012-96-8
DBGET integrated database retrieval system