copalyl diphosphate synthase

Isomerases;
Intramolecular lyases;
Intramolecular lyases (only sub-subclass identified to date)

(+)-copalyl-diphosphate lyase (decyclizing)

geranylgeranyl diphosphate = (+)-copalyl diphosphate [RN:R06298]

R06298

geranylgeranyl diphosphate [CPD:C00353]

In some plants, such as Salvia miltiorrhiza, this enzyme is monofunctional. In other plants this activity is often a part of a bifunctional enzyme. For example, in Selaginella moellendorffii this activity is catalysed by a bifunctional enzyme that also catalyses EC 4.2.3.131, miltiradiene synthase, while in the tree Abies grandis (grand fir) it is catalysed by a bifunctional enzyme that also catalyses EC 4.2.3.18, abietadiene synthase.

1  [PMID:11552804]

Peters RJ, Ravn MM, Coates RM, Croteau RB.

Bifunctional abietadiene synthase: free diffusive transfer of the (+)-copalyl diphosphate intermediate between two distinct active sites.

J. Am. Chem. Soc. 123 (2001) 8974-8.

Abies grandis

2  [PMID:22027823]

Sugai Y, Ueno Y, Hayashi K, Oogami S, Toyomasu T, Matsumoto S, Natsume M, Nozaki H, Kawaide H

Enzymatic (13)C labeling and multidimensional NMR analysis of miltiradiene synthesized by bifunctional diterpene cyclase in Selaginella moellendorffii.

J. Biol. Chem. 286 (2011) 42840-7.

3  [PMID:11805316]

Peters RJ, Croteau RB.

Abietadiene synthase catalysis: mutational analysis of a prenyl diphosphate ionization-initiated cyclization and rearrangement.

Proc. Natl. Acad. Sci. U. S. A. 99 (2002) 580-4.

Abies grandis

4  [PMID:12059223]

Ravn MM, Peters RJ, Coates RM, Croteau R.

Mechanism of abietadiene synthase catalysis: stereochemistry and stabilization of the cryptic pimarenyl carbocation intermediates.

J. Am. Chem. Soc. 124 (2002) 6998-7006.

Abies grandis

5  [PMID:11827528]

Peters RJ, Croteau RB.

Abietadiene synthase catalysis: conserved residues involved in protonation-initiated cyclization of geranylgeranyl diphosphate to (+)-copalyl diphosphate.

Biochemistry. 41 (2002) 1836-42.

Abies grandis