| Entry |
|
|---|
| Name |
glutathionylspermidine synthase;
glutathione:spermidine ligase (ADP-forming) |
|---|
| Class |
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-ammonia (or amine) ligases (amide synthases)
 |
|---|
| Sysname |
gamma-L-glutamyl-L-cysteinyl-glycine:spermidine ligase (ADP-forming)
[spermidine is numbered so that atom N-1 is in the amino group of
the aminopropyl part of the molecule] |
|---|
| Reaction(IUBMB) |
glutathione + spermidine + ATP = glutathionylspermidine + ADP +
phosphate [RN:R01917] |
|---|
| Reaction(KEGG) |
R01917
 |
|---|
| Substrate |
glutathione [CPD:C00051];
spermidine [CPD:C00315];
ATP [CPD:C00002] |
|---|
| Product |
glutathionylspermidine [CPD:C05730];
ADP [CPD:C00008];
phosphate [CPD:C00009] |
|---|
| Cofactor |
Magnesium [CPD:C00305] |
|---|
| Comment |
Requires magnesium ions. Involved in the synthesis of trypanothione
in trypanosomatids. The enzyme from Escherichia coli is bifunctional
and also catalyses the glutathionylspermidine amidase (EC 3.5.1.78)
reaction, resulting in a net hydrolysis of ATP. |
|---|
| Pathway |
PATH: ec00480 Glutathione metabolism
PATH: ec01100 Metabolic pathways |
|---|
| Orthology |
KO: K01460 glutathionylspermidine amidase/synthetase
KO: K01917 glutathionylspermidine synthase |
|---|
| Genes |
ECO: b2988(gsp)
ECJ: JW2956(gss)
ECD: ECDH10B_3165(gss)
EBW: BWG_2706(gsp)
ECE: Z4342(gsp)
ECS: ECs3873
ECF: ECH74115_4300(gsp)
ETW: ECSP_3967(gsp)
ECG: E2348C_3275(gsp)
ECC: c3725(gsp)
ECI: UTI89_C3410(gsp)
ECP: ECP_3073
ECV: APECO1_3433(gsp)
ECW: EcE24377A_3455(gsp)
ECX: EcHS_A3168(gsp)
ECM: EcSMS35_3274(gsp)
ECY: ECSE_3273
ECL: EcolC_0705
ECK: EC55989_3404(gss)
ECQ: ECED1_3638(gss)
ECR: ECIAI1_3137(gss)
ECT: ECIAI39_3484(gss)
ECZ: ECS88_3370(gss)
EUM: ECUMN_3472(gss)
ELF: LF82_0937(gsp)
EBL: B21_02813(gss)
EBD: ECBD_0750
EBR: ECB_02864(gss)
EOH: ECO103_3672(gss)
EOI: ECO111_3818(gss)
EOJ: ECO26_4096(gss)
EFE: EFER_2930(gss)
STY: STY3310(gsp)
STT: t3060(gsp)
SPT: SPA3007(gsp)
SEK: SSPA2805
SPQ: SPAB_03921
SEI: SPC_3208(gsp)
SEC: SC3080(gsp)
SEH: SeHA_C3386
SEE: SNSL254_A3392
SEW: SeSA_A3321
SEA: SeAg_B3312
SED: SeD_A3486
SET: SEN2982(gsp)
SES: SARI_04484
STM: STM3139(gsp)
SFL: SF3035(gsp)
SFX: S3236(gsp)
SFV: SFV_3041(gsp)
SSN: SSON_3133(gsp)
SBO: SBO_2875(gsp)
SBC: SbBS512_E3415(gsp)
SDY: SDY_3085(gsp)
ENT: Ent638_3393
KPN: KPN_03416(gss)
KPE: KPK_0702(gsp)
KPU: KP1_4691(gss)
CKO: CKO_04379
PMR: PMI0183(gsp)
EIC: NT01EI_2998
ETR: ETAE_2690
MSU: MS0671(gsp)
ASU: Asuc_0804
AAP: NT05HA_2010
AAT: D11S_0204
PHA: PSHAa2455(gssA)
PIN: Ping_1235
MMW: Mmwyl1_0216
REH: H16_A0042
CTI: RALTA_A0008(gsp)
HPY: HP0233
HPJ: jhp0218
HPA: HPAG1_0236
HPS: HPSH_01210
HPG: HPG27_213
HPP: HPP12_0233
HPB: HELPY_0237(gsp)
HHE: HH0015(ygiC)
HAC: Hac_0310
WSU: WS0574
TDN: Suden_1471
CJE: Cj0372
CJR: CJE0421
CJJ: CJJ81176_0396
CJU: C8J_0348
CJD: JJD26997_1585
CFF: CFF8240_1661
CCV: CCV52592_1541
CCO: CCC13826_1647
CLA: Cla_1553
ABU: Abu_1068
SUN: SUN_0613
BRA: BRADO1482
BBT: BBta_6550
BAN: BA1602 BA2932
BAR: GBAA1602 GBAA2932
BAA: BA_2120 BA_3442
BAT: BAS1486 BAS2724
BAH: BAMEG_1672 BAMEG_2989
BAI: BAA_2986
BCE: BC1579
BCA: BCE_2970
BCZ: BCZK1458 BCZK2652(gspS)
BCR: BCAH187_A1750 BCAH187_A2978
BCB: BCB4264_A1639
BCU: BCAH820_1672 BCAH820_2931 BCAH820_B0141
BCG: BCG9842_0028 BCG9842_B3706
BCQ: BCQ_1644 BCQ_2762(gspS)
BCX: BCA_1642 BCA_3004
BCY: Bcer98_1313
BTK: BT9727_1459 BT9727_2675(gspS)
BTL: BALH_1426 BALH_2622
BWE: BcerKBAB4_1501
GWC: GWCH70_1786
ESI: Exig_2920
EAT: EAT1b_1413
BBE: BBR47_42720
PJD: Pjdr2_2187
AVA: Ava_B0291
 |
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| Structures |
PDB: 2IO7 2IO8 2IO9 2IOA 2IOB |
|---|
Reference
Authors
Title
Journal
Organism
|
1 [PMID:1304372]
Smith K, Nadeau K, Bradley M, Walsh C, Fairlamb AH.
Purification of glutathionylspermidine and trypanothione synthetases
from Crithidia fasciculata.
Protein. Sci. 1 (1992) 874-83.
Crithidia fasciculata |
|---|
Reference
Authors
Title
Journal
Organism
Sequence
|
2 [PMID:7775463]
Bollinger JM Jr, Kwon DS, Huisman GW, Kolter R, Walsh CT.
Glutathionylspermidine metabolism in Escherichia coli. Purification,
cloning, overproduction, and characterization of a bifunctional
glutathionylspermidine synthetase/amidase.
J. Biol. Chem. 270 (1995) 14031-41.
Escherichia coli [GN:eco]
ECO: b2988 |
|---|
| Other DBs |
ExplorEnz - The Enzyme Database: 6.3.1.8
IUBMB Enzyme Nomenclature: 6.3.1.8
ExPASy - ENZYME nomenclature database: 6.3.1.8
BRENDA, the Enzyme Database: 6.3.1.8
CAS: 9077-09-2 |
|---|
