KEGG   ENZYME: 6.3.2.26Help
Entry
EC 6.3.2.26                 Enzyme                                 
Name
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase;
L-delta-(alpha-aminoadipoyl)-L-cysteinyl-D-valine synthetase;
ACV synthetase;
L-alpha-aminoadipyl-cysteinyl-valine synthetase
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)
BRITE hierarchy
Sysname
L-2-aminohexanedioate:L-cysteine:L-valine ligase (AMP-forming, valine-inverting)
Reaction(IUBMB)
3 ATP + L-2-aminohexanedioate + L-cysteine + L-valine + H2O = 3 AMP + 3 diphosphate + N-[L-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine [RN:R04870]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
L-2-aminohexanedioate [CPD:C00956];
L-cysteine [CPD:C00097];
L-valine [CPD:C00183];
H2O [CPD:C00001]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
N-[L-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine [CPD:C05556]
Comment
Requires Mg2+. The enzyme contains 4'-phosphopantetheine, which may be involved in the mechanism of the reaction. Forms part of the penicillin biosynthesis pathway (for pathway, click here).
Pathway
Penicillin and cephalosporin biosynthesis
Metabolic pathways
Biosynthesis of secondary metabolites
Orthology
K12743  
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase
Genes
ANI: 
AOR: 
AOR_1_920074(AO090038000543)
AFV: 
Taxonomy
Reference
1  [PMID:11851475]
  Authors
Byford MF, Baldwin JE, Shiau CY, Schofield CJ.
  Title
The Mechanism of ACV Synthetase.
  Journal
Chem. Rev. 97 (1997) 2631-2650.
  Organism
Cephalosporium acremonium
Reference
2  [PMID:9355751]
  Authors
Theilgaard HB, Kristiansen KN, Henriksen CM, Nielsen J.
  Title
Purification and characterization of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase from Penicillium chrysogenum.
  Journal
Biochem. J. 327 ( Pt 1) (1997) 185-91.
  Organism
Penicillium chrysogenum [GN:pcs]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
57219-73-5
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