| Entry |
|
|---|
| Name |
aerobactin synthase;
citrate:6-N-acetyl-6-N-hydroxy-L-lysine ligase (ADP-forming) |
|---|
| Class |
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)
 |
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| Sysname |
citrate:N6-acetyl-N6-hydroxy-L-lysine ligase (ADP-forming) |
|---|
| Reaction(IUBMB) |
4 ATP + citrate + 2 N6-acetyl-N6-hydroxy-L-lysine + 2 H2O = 4 ADP +
4 phosphate + aerobactin [RN:R04357] |
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| Reaction(KEGG) |
R04357
 |
|---|
| Substrate |
ATP [CPD:C00002];
citrate [CPD:C00158];
N6-acetyl-N6-hydroxy-L-lysine [CPD:C03955];
H2O [CPD:C00001] |
|---|
| Product |
ADP [CPD:C00008];
phosphate [CPD:C00009];
aerobactin [CPD:C05554] |
|---|
| Comment |
Requires Mg2+. Aerobactin is one of a group of high-affinity iron
chelators known as siderophores and is produced under conditions of
iron deprivation [5]. It is a dihydroxamate comprising two molecules
of N6-acetyl-N6-hydroxylysine and one molecule of citric acid. This
is the last of the three enzymes involved in its synthesis, the
others being EC 1.14.13.59, L-lysine 6-monooxygenase (NADPH) and EC
2.3.1.102, N6-hydroxylysine O-acetyltransferase [3]. |
|---|
| Pathway |
PATH: ec00310 Lysine degradation |
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| Orthology |
KO: K03894 aerobactin synthetase subunit alpha
KO: K03895 aerobactin synthetase subunit beta |
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| Genes |
ECC: c3625(iucC) c3627(iucA)
ECV: APECO1_O1CoBM170(iucC) APECO1_O1CoBM172(iucA)
YPE: YPO0992(iucC)
YPK: y3380(iucA1) y3381(iucA2) y3383(iucC)
YPM: YP_3448(iucC)
YPA: YPA_0272 YPA_0274 YPA_0275
YPN: YPN_3187 YPN_3188 YPN_3190
YPP: YPDSF_0526 YPDSF_0528 YPDSF_0529
YPS: YPTB3263(rhbC) YPTB3265(iucC)
SFL: SF3715(iucA) SF3717(iucC)
SFX: S4054(iucC) S4056(iucA)
SFV: SFV_3852(iucC) SFV_3854(iucA)
SSN: SSON_3601(iucA) SSON_3603(iucC)
SBO: SBO_4337(iucA) SBO_4339(iucC)
SBC: SbBS512_E4632(iucC) SbBS512_E4634(iucA)
ESA: ESA_pESA3p05547 ESA_pESA3p05549
CKO: CKO_00971 CKO_00974
SPE: Spro_0920 Spro_0922
VFI: VF_A0161(iucA) VF_A0163(iucC)
NOC: Noc_1812 Noc_1814
BBA: Bd1574(iucC) Bd1576(iucA)
GBE: GbCGDNIH1_2376 GbCGDNIH1_2378
SAO: SAOUHSC_00079 SAOUHSC_00080
FRA: Francci3_4058
 |
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Reference
Authors
Title
Journal
|
1
Appanna, D.L., Grundy, B.J., Szczepan, E.W. and Viswanatha, T.
Aerobactin synthesis in a cell-free system of Aerobacter aerogenes
62-1.
Biochim. Biophys. Acta 801 (1984) 437-443. |
|---|
Reference
Authors
Title
Journal
Organism
|
2 [PMID:4313071]
Gibson F, Magrath DI.
The isolation and characterization of a hydroxamic acid (aerobactin)
formed by Aerobacter aerogenes 62-I.
Biochim. Biophys. Acta. 192 (1969) 175-84.
Aerobacter aerogenes |
|---|
Reference
Authors
Title
Journal
|
3
Maurer, P.J. and Miller, M.
Microbial iron chelators: total synthesis of aerobactin and its
constituent amino acid, N6-acetyl-N6-hydroxylysine.
J. Am. Chem. Soc. 104 (1982) 3096-3101. |
|---|
Reference
Authors
Title
Journal
Organism
|
4 [PMID:2935523]
de Lorenzo V, Bindereif A, Paw BH, Neilands JB.
Aerobactin biosynthesis and transport genes of plasmid ColV-K30 in
Escherichia coli K-12.
J. Bacteriol. 165 (1986) 570-8.
Escherichia coli [GN:eco] |
|---|
Reference
Authors
Title
Journal
Organism
|
5 [PMID:15719346]
Challis GL.
A widely distributed bacterial pathway for siderophore biosynthesis
independent of nonribosomal peptide synthetases.
Chembiochem. 6 (2005) 601-11.
Escherichia coli [GN:eco] |
|---|
| Other DBs |
ExplorEnz - The Enzyme Database: 6.3.2.27
IUBMB Enzyme Nomenclature: 6.3.2.27
ExPASy - ENZYME nomenclature database: 6.3.2.27
BRENDA, the Enzyme Database: 6.3.2.27
CAS: 94047-30-0 |
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