| Entry |
|
|---|
| Name |
adenosylcobyric acid synthase (glutamine-hydrolysing);
CobQ;
cobyric acid synthase;
5'-deoxy-5'-adenosylcobyrinic-acid-a,c-diamide:L-glutamine
amido-ligase;
Ado-cobyric acid synthase [glutamine hydrolyzing] |
|---|
| Class |
Ligases;
Forming carbon-nitrogen bonds;
Carbon-nitrogen ligases with glutamine as amido-N-donor
 |
|---|
| Sysname |
adenosylcobyrinic-acid-a,c-diamide:L-glutamine amido-ligase
(ADP-forming) |
|---|
| Reaction(IUBMB) |
4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O =
4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate
[RN:R05225] |
|---|
| Reaction(KEGG) |
R05225
 |
|---|
| Substrate |
ATP [CPD:C00002];
adenosylcobyrinic acid a,c-diamide [CPD:C06506];
L-glutamine [CPD:C00064];
H2O [CPD:C00001] |
|---|
| Product |
ADP [CPD:C00008];
phosphate [CPD:C00009];
adenosylcobyric acid [CPD:C06507];
L-glutamate [CPD:C00025] |
|---|
| Comment |
Requires Mg2+. NH3 can act instead of glutamine. This enzyme
catalyses the four-step amidation sequence from cobyrinic acid
a,c-diamide to cobyric acid via the formation of cobyrinic acid
triamide, tetraamide and pentaamide intermediates. |
|---|
| Pathway |
PATH: ec00860 Porphyrin and chlorophyll metabolism
PATH: ec01100 Metabolic pathways |
|---|
| Orthology |
KO: K02232 adenosylcobyric acid synthase |
|---|
| Genes |
STY: STY2222(cbiP)
STT: t0855(cbiP)
SPT: SPA0852(cbiP)
SEK: SSPA0797
SPQ: SPAB_01086
SEI: SPC_1696(cbiP)
SEC: SC2027(cbiP)
SEH: SeHA_C2241(cobQ)
SEE: SNSL254_A2195(cobQ)
SEW: SeSA_A2188(cobQ)
SEA: SeAg_B2141(cobQ)
SED: SeD_A2354(cobQ)
SEG: SG2043(cbiP)
SET: SEN2017(cbiP)
SES: SARI_00869
STM: STM2019(cbiP)
YEN: YE2711(cbiP)
PLU: plu2987(cbiP)
PAY: PAU_01613(cobQ)
KPN: KPN_03184(cbiP)
KPE: KPK_0932(cobQ)
KPU: KP1_4449
CKO: CKO_00819
EIC: NT01EI_1850 NT01EI_2180
ETR: ETAE_1848(cbiP1)
XCC: XCC3061
XCB: XC_1097
XCA: xccb100_1134
XCV: XCV3317(cobQ)
XAC: XAC3188(cobQ)
VCH: VCA0727
VCO: VC0395_0666(cobQ)
VCM: VCM66_A0685(cobQ)
VCJ: VCD_000592
VVU: VV2_0330
VVY: VVA0824
VPA: VPA0932
VHA: VIBHAR_05724
VSP: VS_II0722
VEX: VEA_000116
VFI: VF_A0289(cobQ)
VFM: VFMJ11_A0326(cobQ)
VSA: VSAL_II0783(cobQ)
PPR: PBPRB0991
PAE: PA1277(cobQ)
PAU: PA14_47690(cobQ)
PAP: PSPA7_4113(cobQ)
PAG: PLES_40351(cobQ)
PPU: PP_1677(cobQ)
PPF: Pput_4042
PPG: PputGB1_1276
PPW: PputW619_1236
PST: PSPTO_1713(cobQ)
PSB: Psyr_3676
PSP: PSPPH_3697(cobQ)
PFL: PFL_4426(cobQ)
PFO: Pfl01_1646
PFS: PFLU4486
PEN: PSEEN1383
PMY: Pmen_1755
PSA: PST_1297(cobQ)
CJA: CJA_3773(cobQ)
AVN: Avin_33080(cobQ)
ACI: ACIAD1071(cobQ)
SON: SO_1038(cobQ)
SFR: Sfri_0879
SAZ: Sama_0765
SBL: Sbal_3361
SBM: Shew185_0980
SBN: Sbal195_1014
SBP: Sbal223_1002
SLO: Shew_0717
SPC: Sputcn32_2958
SSE: Ssed_2157 Ssed_3731
SPL: Spea_3377
SHE: Shewmr4_0845
SHM: Shewmr7_3177
SHN: Shewana3_3275
SHW: Sputw3181_0989
SHL: Shal_3454
SWD: Swoo_4081
SWP: swp_0999
CPS: CPS_3655(cobQ)
PHA: PSHAa2564(cobQ)
PAT: Patl_1135
SDE: Sde_0240
MAQ: Maqu_0279
TTU: TERTU_3614(cobQ)
MCA: MCA2304(cobQ)
AEH: Mlg_2812
HHA: Hhal_1907
HCH: HCH_00962(cobQ)
ABO: ABO_2365(cobQ)
MMW: Mmwyl1_3678
TAU: Tola_1665 Tola_3117
CVI: CV_1576(cobQ)
RSO: RSc2390(cobQ)
RPI: Rpic_2656
RPF: Rpic12D_2198
REU: Reut_A0665
REH: H16_A2961(cbiP)
RME: Rmet_2778
CTI: RALTA_A2435(cobQ)
BMA: BMA0697(cobQ)
BMV: BMASAVP1_A2316(cobQ)
BML: BMA10229_A2971(cobQ)
BMN: BMA10247_1628(cobQ)
BPS: BPSL0987(cobQ)
BPM: BURPS1710b_1200(cobQ)
BPL: BURPS1106A_1045(cobQ)
BPD: BURPS668_1039(cobQ)
BPR: GBP346_A1038(cobQ)
BTE: BTH_I0844(cobQ)
BVI: Bcep1808_2530
BUR: Bcep18194_A5777
BCN: Bcen_1835
BCH: Bcen2424_2446
BCM: Bcenmc03_2451
BCJ: BCAL2656(cobQ)
BAM: Bamb_2494
BAC: BamMC406_2362
BMU: Bmul_0848
BMJ: BMULJ_02410(cobQ)
BXE: Bxe_A3492
BPH: Bphy_2203
BPY: Bphyt_1005
BGL: bglu_1g28120
RFR: Rfer_2612
POL: Bpro_2760
PNA: Pnap_2146
AAV: Aave_1552
AJS: Ajs_3178
VEI: Veis_4778
DAC: Daci_2806
DIA: Dtpsy_2529
VAP: Vapar_2565
CTT: CtCNB1_1854
MPT: Mpe_A2304(copQ) Mpe_B0518(cobQ)
HAR: HEAR0970(cobQ)
MMS: mma_1101(cobQ)
LCH: Lcho_2661
EBA: ebA3690(cobQ)
AZO: azo3517
DAR: Daro_0144
TBD: Tbd_2714
MFA: Mfla_0103
MMB: Mmol_0269
MEI: Msip34_0142
APP: CAP2UW1_1382
GSU: GSU2992(cobQ)
GME: Gmet_0485
GUR: Gura_0041
GLO: Glov_3554
GBM: Gbem_3539
GEO: Geob_0038
GEM: GM21_3605
PCA: Pcar_0484
PPD: Ppro_0871
DVU: DVU0816(cobQ)
DVL: Dvul_2161
DVM: DvMF_2864
DDE: Dde_1030
DDS: Ddes_2177
DMA: DMR_02680
DSA: Desal_3482
DBA: Dbac_3448
DPS: DP1950
DOL: Dole_1734
DAL: Dalk_0453
DAT: HRM2_02240(cobQ)
SCL: sce0210(cbiP)
SFU: Sfum_1739
MLO: mll1198
SME: SMc04309(cobQ)
SMD: Smed_1765
ATU: Atu2808(cbiP)
ATC: AGR_C_5093
ARA: Arad_2802(cobQ)
AVI: Avi_2571(cbiP)
RET: RHE_CH02494(cobQ)
REC: RHECIAT_CH0002598(cobQ)
RLE: RL2836(cobQ)
RLT: Rleg2_2137
RLG: Rleg_2381
RHI: NGR_c18240
BME: BMEI0690
BMF: BAB1_1331
BMB: BruAb1_1312(cobQ)
BMC: BAbS19_I12440
BMS: BR1311(cobQ)
BMT: BSUIS_A1362(cobQ)
BOV: BOV_1274(cobQ)
BCS: BCAN_A1335(cobQ)
BMR: BMI_I1324(cobQ)
OAN: Oant_1872
BJA: bll3259(cbiP)
BRA: BRADO4913(cobQ)
BBT: BBta_3138(cobQ)
RPA: RPA0718(cobQ1) RPA2096(cobQ2)
RPB: RPB_0736 RPB_3285
RPC: RPC_3745 RPC_4257
RPD: RPD_0634 RPD_2918
RPE: RPE_2238 RPE_3122
RPT: Rpal_0789 Rpal_2386
XAU: Xaut_3778
AZC: AZC_2258(cobQ)
MEX: Mext_4723
MEA: Mex_1p5174(cobQ)
MDI: METDI5776(cobQ1)
MRD: Mrad2831_1471
MET: M446_2427
MPO: Mpop_5262
MCH: Mchl_5190 Mchl_5723
MNO: Mnod_2141
BID: Bind_0387
MSL: Msil_3272
HCI: HCDSEM_001(cobQ)
CCR: CC_1751 CC_2694
CCS: CCNA_01827 CCNA_02776
CAK: Caul_2617
SIL: SPO1242(cobQ)
SIT: TM1040_1989
RSP: RSP_0796(cobQ)
RSH: Rsph17029_2453 Rsph17029_3563 Rsph17029_3564
RSQ: Rsph17025_0384
RSK: RSKD131_2158 RSKD131_4281 RSKD131_4282
JAN: Jann_2937
RDE: RD1_2180(cobQ)
PDE: Pden_3713
DSH: Dshi_2096(cobQ)
HNE: HNE_2169(cobQ)
HBA: Hbal_2159
NAR: Saro_0323
SAL: Sala_2756
SWI: Swit_4734
GBE: GbCGDNIH1_0654
GDI: GDI_0034(cobQ)
GDJ: Gdia_1656
APT: APA01_22480(cobQ)
RRU: Rru_A3381 Rru_A3549 Rru_A3550
RCE: RC1_0321(cobQ)
MAG: amb0292
MGM: Mmc1_3127
BHA: BH1591(cobQ)
GKA: GK1795
GTN: GTNG_1684(cbiP)
GWC: GWCH70_1560
GYM: GYMC10_2017
GYC: GYMC61_2606
AFL: Aflv_2170
LMO: lmo1208(cbiP)
LMF: LMOf2365_1217(cobQ)
LMH: LMHCC_1443(cbiP)
LMC: Lm4b_01212(cbiP)
LIN: lin1171
LWE: lwe1165(cbiP)
LSP: Bsph_3430
BBE: BBR47_12550(cbiP)
PJD: Pjdr2_3625
SSA: SSA_0481
LRE: Lreu_1705
LRF: LAR_1593
CAC: CAC1374(cbiP)
CPE: CPE1045(cbiP)
CPF: CPF_1300(cobQ)
CPR: CPR_1119(cobQ)
CTC: CTC00720(cbiP)
CNO: NT01CX_2081(cobQ)
CDF: CD3435(cbiP)
CDC: CD196_3211(cbiP)
CDL: CDR20291_3257(cbiP)
CBO: CBO0914(cbiP)
CBA: CLB_0954(cobQ)
CBH: CLC_0968(cobQ)
CBY: CLM_1061(cobQ)
CBL: CLK_0351(cobQ)
CBK: CLL_A2917(cobQ)
CBB: CLD_3646(cobQ)
CBI: CLJ_B0964(cobQ)
CBT: CLH_2659(cobQ)
CBF: CLI_1000(cobQ)
CBE: Cbei_1272
CKL: CKL_0721(cbiP)
CKR: CKR_0643
CPY: Cphy_1112
CCE: Ccel_1284
AMT: Amet_3616
AOE: Clos_0863
STH: STH1932
VPR: Vpar_0906
DSY: DSY4062(cobQ)
DHD: Dhaf_1304
DRM: Dred_2705
DAE: Dtox_3093
PTH: PTH_0958(cobQ)
DAU: Daud_1848
HMO: HM1_2397(cobQ)
EEL: EUBELI_20173
ERE: EUBREC_1926
TTE: TTE0376(cobQ)
TEX: Teth514_0300
TPD: Teth39_1900
CHY: CHY_0769(cobQ)
MTA: Moth_1098
ADG: Adeg_0181
NTH: Nther_0944
MTU: Rv0255c(cobQ1)
MTC: MT0268(cobQ-1)
MRA: MRA_0264(cobQ1)
MTF: TBFG_10258
MTB: TBMG_00257(TBMG_00257.1)
MBO: Mb0261c(cobQ1)
MBB: BCG_0293c(cobQ1)
MBT: JTY_0262(cobQ1)
MPA: MAP2933c(cobQ)
MAV: MAV_3720(cobQ)
MSM: MSMEG_2588(cobQ)
MUL: MUL_3770(cobQ1)
MVA: Mvan_0377
MGI: Mflv_0360
MAB: MAB_3163c
MMC: Mmcs_2051
MKM: Mkms_2097
MJL: Mjls_2034
MMI: MMAR_3838(cobQ1)
CEF: CE1900
CDI: DIP1495(cobQ)
CKP: ckrop_1158(cobQ)
NFA: nfa40880
RHA: RHA1_ro06605
RER: RER_25900(cobQ)
ROP: ROP_66390(cobQ)
GBR: Gbro_2128
SCO: SCO1848(cobQ)
SMA: SAV_6416(cobQ1)
SGR: SGR_5644
PAC: PPA0419
NCA: Noca_2509 Noca_2880
TFU: Tfu_0309
TCU: Tcur_1288
SRO: Sros_7399
FRA: Francci3_2603
FRE: Franean1_4863
FAL: FRAAL2887(bioD)
NML: Namu_0138
SEN: SACE_5983(cobQ)
SVI: Svir_17700
AMI: Amir_5883
STP: Strop_3554
SAQ: Sare_3928
CAI: Caci_6023
RXY: Rxyl_0634
AFO: Afer_0815
SHI: Shel_21140
TDE: TDE2326
LIL: LB151(cbiP)
LIC: LIC20121(cbiP)
LBJ: LBJ_4191(cobQ)
LBL: LBL_4206(cobQ)
BFR: BF2491
BFS: BF2524(cbiP)
BVU: BVU_3115
PGN: PGN_1260
PDI: BDI_1360
DFE: Dfer_5369
CPI: Cpin_4467
FNU: FN0977 FN2070
LBA: Lebu_0043
STR: Sterm_1018
OTE: Oter_3692
AMU: Amuc_1683
GAU: GAU_0498(cobQ)
SYN: slr0618(cbiP)
SYW: SYNW1704(cobB)
SYC: syc1301_c(cobQ)
SYF: Synpcc7942_0211
SYD: Syncc9605_0766
SYE: Syncc9902_1596
SYG: sync_0893(cobQ)
SYR: SynRCC307_1699(cobQ)
SYX: SynWH7803_1658(cobQ)
SYP: SYNPCC7002_A1650(cobQ)
CYA: CYA_2678(cobQ)
CYB: CYB_2173(cobQ)
TEL: tll1716(cobQ)
MAR: MAE_57120(cobQ)
CYT: cce_3359(cobQ)
CYP: PCC8801_1628
CYC: PCC7424_3640
CYN: Cyan7425_2747
CYH: Cyan8802_1655
GVI: gvip245(cobQ)
ANA: alr2377
NPU: Npun_F0495
AVA: Ava_0196
PMA: Pro1259(cobQ)
PMM: PMM1160(cobB)
PMT: PMT1183(cobB)
PMN: PMN2A_0771
PMI: PMT9312_1259
PMB: A9601_13371(cobQ)
PMC: P9515_13271(cobQ)
PMF: P9303_08361(cobQ)
PMG: P9301_13521(cobQ)
PMH: P9215_13661(cobQ)
PMJ: P9211_12371(cobQ)
PME: NATL1_16121(cobQ)
TER: Tery_3957
AMR: AM1_4245(cobQ)
CTE: CT0938(cbiP)
CPC: Cpar_1181
CCH: Cag_1012
CPH: Cpha266_1117
CPB: Cphamn1_1516
CLI: Clim_1066
PVI: Cvib_0814
PLT: Plut_1140
PPH: Ppha_1264
PAA: Paes_1269
CTS: Ctha_1276
DET: DET0936(cobQ)
DEH: cbdb_A890(cobQ)
DEB: DehaBAV1_0820
DEV: DhcVS_807(cobQ)
RRS: RoseRS_0577
RCA: Rcas_0632
CAU: Caur_2560
CAG: Cagg_1259
CHL: Chy400_2767
HAU: Haur_1051
DRA: DR_B0012
DGE: Dgeo_2874
DDR: Deide_2p01820(cobQ)
TTH: TT_P0023
TTJ: TTHB066
TME: Tmel_0858
TAF: THA_1042(cobQ)
FNO: Fnod_1369
TYE: THEYE_A1177(cobQ)
MJA: MJ0484
MFE: Mefer_1585
MVU: Metvu_0505
MMP: MMP1215
MMQ: MmarC5_0375
MMX: MmarC6_1457
MMZ: MmarC7_0462
MAE: Maeo_1252
MVN: Mevan_0530
MAC: MA3250
MBA: Mbar_A3191
MMA: MM_0093
MBU: Mbur_2361
MTP: Mthe_0385
MHU: Mhun_2912
MEM: Memar_1999
MBN: Mboo_1901
MPL: Mpal_1991
MTH: MTH787
MST: Msp_1226(cobQ)
MSI: Msm_1565
MKA: MK1414(cobQ)
AFU: AF1338(cbiP)
HAL: VNG1576G(cbiP)
HSL: OE3246F(cbiP)
HMA: rrnAC1938(cobQ)
HWA: HQ1404A(cbiP)
NPH: NP5126A(cbiP)
HLA: Hlac_0238
HUT: Huta_2504
HMU: Hmuk_1854
TAC: Ta0075
TVO: TVN0031
PTO: PTO0812
PFU: PF0301
TKO: TK0854
TON: TON_0687
TGA: TGAM_0535(cobQ)
RCI: RCIX2662(cobQ)
SSO: SSO3233
STO: ST2344
SAI: Saci_0413
SIS: LS215_2296
SIA: M1425_2133
SIM: M1627_2213
SID: M164_2137
SIY: YG5714_2260
SIN: YN1551_0656
MSE: Msed_0199
CMA: Cmaq_0251
NMR: Nmar_1584
 |
|---|
Reference
Authors
Title
Journal
Organism
|
1 [PMID:1917839]
Blanche F, Couder M, Debussche L, Thibaut D, Cameron B, Crouzet J.
Biosynthesis of vitamin B12: stepwise amidation of carboxyl groups
b, d, e, and g of cobyrinic acid a,c-diamide is catalyzed by one
enzyme in Pseudomonas denitrificans.
J. Bacteriol. 173 (1991) 6046-51.
Pseudomonas denitrificans |
|---|
Reference
Authors
Title
Journal
Organism
|
2 [PMID:12195810]
Warren MJ, Raux E, Schubert HL, Escalante-Semerena JC.
The biosynthesis of adenosylcobalamin (vitamin B12).
Nat. Prod. Rep. 19 (2002) 390-412.
Pseudomonas denitrificans |
|---|
| Other DBs |
ExplorEnz - The Enzyme Database: 6.3.5.10
IUBMB Enzyme Nomenclature: 6.3.5.10
ExPASy - ENZYME nomenclature database: 6.3.5.10
BRENDA, the Enzyme Database: 6.3.5.10
CAS: 137672-90-3 |
|---|
