KEGG   ENZYME: 6.6.1.2Help
Entry
EC 6.6.1.2                  Enzyme                                 
Name cobaltochelatase;
hydrogenobyrinic acid a,c-diamide cobaltochelatase;
CobNST;
CobNCobST
Class Ligases;
Forming nitrogen-D-metal bonds;
Forming coordination complexes
BRITE hierarchy
Sysname hydrogenobyrinic-acid-a,c-diamide:cobalt cobalt-ligase (ADP-forming)
Reaction(IUBMB) ATP + hydrogenobyrinic acid a,c-diamide + Co2+ + H2O = ADP +
phosphate + cob(II)yrinic acid a,c-diamide + H+ [RN:R05227]
Reaction(KEGG) R05227
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Substrate ATP [CPD:C00002];
hydrogenobyrinic acid a,c-diamide [CPD:C06503];
Co2+ [CPD:C00175];
H2O [CPD:C00001]
Product ADP [CPD:C00008];
phosphate [CPD:C00009];
cob(II)yrinic acid a,c-diamide [CPD:C06504];
H+ [CPD:C00080]
Comment This enzyme, which forms part of the aerobic cobalamin biosynthesis
pathway, is a type I chelatase, being heterotrimeric and
ATP-dependent. It comprises two components, one of which corresponds
to CobN and the other is composed of two polypeptides, specified by
cobS and cobT in Pseudomonas denitrificans, and named CobST [1].
Hydrogenobyrinic acid is a very poor substrate. ATP can be replaced
by dATP or CTP but the reaction proceeds more slowly. CobN exhibits
a high affinity for hydrogenobyrinic acid a,c-diamide. The
oligomeric protein CobST possesses at least one sulfhydryl group
that is essential for ATP-binding. Once the Co2+ is inserted, the
next step in the pathway ensures that the cobalt is ligated securely
by reducing Co(II) to Co(I). This step is carried out by EC
1.16.8.1, cob(II)yrinic acid a,c-diamide reductase.
Pathway PATH: ec00860  Porphyrin and chlorophyll metabolism
PATH: ec01100  Metabolic pathways
Orthology KO: K02230  cobaltochelatase CobN
KO: K06050
KO: K09882  cobaltochelatase CobS
KO: K09883  cobaltochelatase CobT
Genes SEE: SNSL254_p_0106
PAE: PA1923(cobN) PA2944(cobN)
PAU: PA14_25970(cobN) PA14_39640(cobN)
PAP: PSPA7_2219(cobN) PSPA7_3363(cobN)
PAG: PLES_21191(cobN) PLES_34001(cobN)
PPU: PP_3507(cobN)
PPF: Pput_2269(cobN)
PPG: PputGB1_2422(cobN)
PPW: PputW619_2773(cobN)
PST: PSPTO_3149
PSB: Psyr_3015(cobN)
PSP: PSPPH_2225(cobN)
PFL: PFL_3654(cobN)
PFO: Pfl01_3098(cobN)
PFS: PFLU2670 pQBR0011
PEN: PSEEN2433(cobN)
PMY: Pmen_4586(cobN)
PSA: PST_1796(cobN)
AVN: Avin_27480
SPC: Sputcn32_2340
SHW: Sputw3181_1668
TTU: TERTU_3326(cobN)
HCH: HCH_06504(cobN)
MMW: Mmwyl1_1377
AFR: AFE_1297
CVI: CV_1571(cobN)
RSO: RSp0626(cobN)
REU: Reut_B3755 Reut_B3756
REH: H16_B1887(cobT2) H16_B1888(cobS2)
CTI: RALTA_B1586(cobT2) RALTA_B1587(cobS2)
BMA: BMA1170
BMV: BMASAVP1_A1615(cobN)
BML: BMA10229_A0277(cobN)
BMN: BMA10247_0883(cobN)
BPS: BPSL1768(cobN)
BPM: BURPS1710b_2099(cobN)
BPL: BURPS1106A_1954(cobN)
BPD: BURPS668_1938(cobN)
BPR: GBP346_A1978(cobN)
BTE: BTH_I2407(cobN)
BVI: Bcep1808_1602(cobN)
BUR: Bcep18194_A4806(cobN) Bcep18194_B1996 Bcep18194_B1997
     Bcep18194_C7219
BCN: Bcen_1177(cobN)
BCH: Bcen2424_1657(cobN)
BCM: Bcenmc03_1631(cobN)
BCJ: BCAL1711(cobN) BCAM1178(cobS) BCAM1179
BAM: Bamb_1557(cobN)
BAC: BamMC406_1576(cobN)
BMU: Bmul_1582(cobN) Bmul_4519 Bmul_4520
BMJ: BMULJ_01661(cobN) BMULJ_03990(cobS) BMULJ_03991(cobT)
BXE: Bxe_B0687 Bxe_B0688 Bxe_B1646(cobN)
BPH: Bphy_3152(cobN) Bphy_6208 Bphy_6209
BPY: Bphyt_4365 Bphyt_4366 Bphyt_5001(cobN) Bphyt_5308
BGL: bglu_1g18520 bglu_2g03110
DAC: Daci_1994 Daci_1995 Daci_5746
DIA: Dtpsy_2685
VAP: Vapar_3827 Vapar_3828
CTT: CtCNB1_1881 CtCNB1_3803 CtCNB1_3804
NEU: NE0757(cobN)
AZO: azo2630(cobN)
TMZ: Tmz1t_0221 Tmz1t_0222
MFA: Mfla_0118
MMB: Mmol_0276
MEI: Msip34_0150
GSU: GSU2158
GUR: Gura_0774
GLO: Glov_0262 Glov_3671
PPD: Ppro_1232 Ppro_1259 Ppro_2082
DVU: DVU2037
DAL: Dalk_3520 Dalk_4060 Dalk_4064
SFU: Sfum_0496
PUB: SAR11_0448(cobT) SAR11_0449(cobS)
MLO: mll3561 mll3563 mlr1377(cobN)
MES: Meso_3069 Meso_3071
PLA: Plav_1712 Plav_1713
SME: SMc00700(cobS) SMc00701(cobT) SMc04303(cobN)
SMD: Smed_1759(cobN) Smed_2559 Smed_2560
ATU: Atu2804(cobN) Atu3621(cobT) Atu3622(cobS)
ATC: AGR_C_5085(cobN) AGR_L_2410
ARA: Arad_2797(cobN) Arad_4228(cobT) Arad_4229(cobS)
AVI: Avi_2647(cobN) Avi_4045(cobT) Avi_4046(cobS)
RET: RHE_CH02489(cobN) RHE_CH03820(cobT) RHE_CH03821(cobS)
REC: RHECIAT_CH0002592(cobN) RHECIAT_CH0004092(cobT)
     RHECIAT_CH0004093(cobS)
RLE: RL2830(cobN) RL4348(cobT) RL4349(cobS)
RLT: Rleg2_2131(cobN) Rleg2_3590 Rleg2_3591
RLG: Rleg_2371 Rleg_3883 Rleg_3884
RHI: NGR_c18190(cobN) NGR_c27170(cobT) NGR_c27180(cobS)
BME: BMEI0049 BMEI0050 BMEI0695(cobN)
BMF: BAB1_1326(cobN) BAB1_2022 BAB1_2023
BMB: BruAb1_1307(cobN) BruAb1_1996 BruAb1_1997
BMC: BAbS19_I12390(cobN) BAbS19_I18950 BAbS19_I18960
BMS: BR1306(cobN) BR2021 BR2022
BMT: BSUIS_A1356(cobN) BSUIS_A1862(cobT) BSUIS_A1863(cobS)
BOV: BOV_1269(cobN) BOV_1945(cobT-1) BOV_1946(cobS-1)
BCS: BCAN_A1329(cobN) BCAN_A2067(cobT) BCAN_A2068(cobS)
BMR: BMI_I1319(cobN) BMI_I2043(cobT) BMI_I2044
OAN: Oant_0914 Oant_0915 Oant_1877(cobN)
BJA: bll0178(cobT) bll0180(cobS) blr3263(cobN)
BRA: BRADO0617(cobS) BRADO0618(cobT) BRADO4907(cobN)
BBT: BBta_3144(cobN) BBta_7564(cobT) BBta_7565(cobS)
RPA: RPA0496(cobT2) RPA0498(cobS2) RPA0716(cobN) RPA2399(cobN)
RPB: RPB_0541 RPB_0543 RPB_0738(cobN)
RPC: RPC_0538 RPC_0540 RPC_1889(cobN)
RPD: RPD_0286 RPD_0287 RPD_0636(cobN)
RPE: RPE_0132 RPE_0135 RPE_2236(cobN)
RPT: Rpal_0497 Rpal_0499 Rpal_0787(cobN) Rpal_2652(cobN)
NWI: Nwi_0403 Nwi_0404
NHA: Nham_0498 Nham_0499
OCA: OCAR_4069(cobT) OCAR_4070(cobS)
BHE: BH15880(cobS) BH15890(cobT2)
BQU: BQ12800(cobS) BQ12810(cobT)
BBK: BARBAKC583_0079(cobT) BARBAKC583_0080(cobS)
BTR: Btr_2554(cobS) Btr_2555(cobT2)
BGR: Bgr_19470(cobS) Bgr_19480(cobT2)
XAU: Xaut_1877 Xaut_1878 Xaut_3286(cobN)
AZC: AZC_3679(cobS) AZC_3680(cobT) AZC_3935(cobN)
MEX: Mext_1439(cobN) Mext_3229 Mext_3230
MEA: Mex_1p1328 Mex_1p3440(cobT) Mex_1p3441(cobS)
MDI: METDI2100 METDI4017(cobT) METDI4018(cobS)
MRD: Mrad2831_3115(cobN) Mrad2831_3698 Mrad2831_3700
MET: M446_2438(cobN) M446_6496 M446_6497
MPO: Mpop_1436(cobN) Mpop_3424 Mpop_3425
MCH: Mchl_1714(cobN) Mchl_3553 Mchl_3554
MNO: Mnod_2149 Mnod_6738 Mnod_6740
BID: Bind_3426 Bind_3427 Bind_3507
MSL: Msil_0317 Msil_0318 Msil_3268
HCI: HCDSEM_003(cobN) HCDSEM_046
CCR: CC_0672 CC_3021
CCS: CCNA_00708 CCNA_03115
CAK: Caul_0711 Caul_4144
PZU: PHZ_c0724 PHZ_c0727
SIL: SPO2853 SPO2855 SPO2863(cobN)
SIT: TM1040_0642 TM1040_0643 TM1040_2210(cobN)
RSP: RSP_1976(cobT) RSP_1977(cobS) RSP_2827(cobN)
RSH: Rsph17029_0684 Rsph17029_0687 Rsph17029_1468(cobN)
RSQ: Rsph17025_1518(cobN) Rsph17025_3073 Rsph17025_3076
RSK: RSKD131_0275 RSKD131_0278 RSKD131_1135
JAN: Jann_2735 Jann_2742 Jann_2931(cobN)
RDE: RD1_3339(cobS) RD1_3340(cobT) RD1_3827(cobN)
PDE: Pden_2189 Pden_2191 Pden_2532(cobN)
DSH: Dshi_0158(cobN) Dshi_2711(cobS2) Dshi_2719(cobT1)
MMR: Mmar10_2280 Mmar10_2287
HNE: HNE_3493(cobT) HNE_3494(cobS)
HBA: Hbal_0788 Hbal_0789
ZMO: ZMO1543 ZMO1544
ZMN: Za10_1661 Za10_1662
NAR: Saro_0342(cobN) Saro_2389 Saro_2891
SAL: Sala_0642 Sala_0644
SWI: Swit_2329 Swit_2331
ELI: ELI_08700
GOX: GOX0050 GOX0051
GBE: GbCGDNIH1_0631 GbCGDNIH1_0632 GbCGDNIH1_0658(cobN)
ACR: Acry_1711 Acry_1712
GDI: GDI_1892(cobT) GDI_1893(cobS)
GDJ: Gdia_0115 Gdia_0116
APT: APA01_11100(cobT) APA01_11110(cobS) APA01_22520(cobN)
RRU: Rru_A0206 Rru_A0207 Rru_A3366(cobN)
RCE: RC1_3074 RC1_3077
MAG: amb3915 amb3916 amb4427(cobN)
AOE: Clos_2328
VPR: Vpar_0072
DSY: DSY3871(cobN)
DAU: Daud_1210 Daud_1219
ADG: Adeg_1646
MTU: Rv2062c(cobN)
MTC: MT2121(cobN)
MRA: MRA_2075(cobN)
MTF: TBFG_12098(cobN)
MTB: TBMG_01920(TBMG_01920.1)
MBO: Mb2088c(cobN)
MBB: BCG_2081c(cobN)
MBT: JTY_2075(cobN)
MPA: MAP1805c(cobN)
MAV: MAV_2437(cobN)
MSM: MSMEG_3864(cobN)
MUL: MUL_2277(cobN)
MVA: Mvan_3426(cobN)
MGI: Mflv_3112(cobN)
MAB: MAB_2201(cobN)
MMC: Mmcs_2496(cobN)
MKM: Mkms_2541(cobN)
MJL: Mjls_2533(cobN)
MMI: MMAR_3037(cobN)
CDI: DIP1229(cobN)
CKP: ckrop_0897(cobN)
NFA: nfa31370(cobN)
RHA: RHA1_ro00171(cobN) RHA1_ro03584 RHA1_ro03585
RER: RER_31410(cobN)
ROP: ROP_02910(cobN) ROP_33530(cobS) ROP_33540(cobT)
GBR: Gbro_2482
SCO: SCO1849(cobN)
SMA: SAV_6415(cobN)
SGR: SGR_3825(cobN)
PAC: PPA0102
NCA: Noca_2878(cobN)
TFU: Tfu_0319(cobN)
TCU: Tcur_1291
SRO: Sros_5591
FRA: Francci3_1518(cobN)
FRE: Franean1_4862(cobN)
FAL: FRAAL2330(cobN)
NML: Namu_0145
SEN: SACE_5955(cobN)
SVI: Svir_17590
AMI: Amir_1784
STP: Strop_2528(cobN)
SAQ: Sare_2710(cobN)
CAI: Caci_6126
RXY: Rxyl_0635(cobN)
AFO: Afer_0816
TDE: TDE0749
BVU: BVU_2194
PGN: PGN_0556
DFE: Dfer_5367
CPI: Cpin_4488
COC: Coch_0753
SYN: sll1890(cobN) slr1211(cobN)
SYW: SYNW1240(cobN)
SYC: syc1407_d(cobN)
SYF: Synpcc7942_0097(cobN)
SYD: Syncc9605_1357
SYE: Syncc9902_1121
SYG: sync_1355(cobN)
SYR: SynRCC307_1207(cobN)
SYX: SynWH7803_1275(cobN)
CYA: CYA_0510 CYA_0724
CYB: CYB_0304 CYB_1111
TEL: tlr0900(cobN)
MAR: MAE_43720(cobN)
CYT: cce_4752(cobN)
CYP: PCC8801_3301
CYC: PCC7424_5234
CYN: Cyan7425_2900
CYH: Cyan8802_2815
GVI: gvip423(cobN)
ANA: alr1689(cobN)
NPU: Npun_R0999(cobN)
AVA: Ava_1167(cobN)
PMA: Pro0957(cobN)
PMM: PMM0879(cobN)
PMT: PMT0727(cobN)
PMN: PMN2A_0331
PMI: PMT9312_0921
PMB: A9601_09821(cobN)
PMC: P9515_09611(cobN)
PMF: P9303_14911(cobN)
PMG: P9301_09801(cobN)
PMH: P9215_10131(cobN)
PMJ: P9211_08851(cobN)
PME: NATL1_10041(cobN)
TER: Tery_0132(cobN)
AMR: AM1_4431(cobN)
CTE: CT0418 CT0422
CLI: Clim_1018 Clim_1022
PAA: Paes_1275 Paes_1279
CTS: Ctha_2441 Ctha_2445
RRS: RoseRS_0558 RoseRS_3099
RCA: Rcas_0648
CAU: Caur_2579
CAG: Cagg_1278
CHL: Chy400_2788
HAU: Haur_1059
DGE: Dgeo_2352
DDR: Deide_2p01670(cobN)
MJA: MJ0908
MFE: Mefer_0187 Mefer_1199
MVU: Metvu_0108 Metvu_1265
MMP: MMP0493(cobN)
MMQ: MmarC5_0820 MmarC5_1481(cobN)
MMX: MmarC6_0141 MmarC6_0395 MmarC6_0756(cobN)
MMZ: MmarC7_1517(cobN) MmarC7_1769
MAE: Maeo_0109(cobN) Maeo_0120
MVN: Mevan_0126 Mevan_1658
MAC: MA0346 MA0384(cobN) MA0385(cobN) MA0872(cobN)
MBA: Mbar_A1771(cobN) Mbar_A2144 Mbar_A3731(cobN)
MMA: MM_1602(cobN) MM_1998(cobN)
MBU: Mbur_0865 Mbur_2260
MTP: Mthe_0434 Mthe_0441 Mthe_0835 Mthe_0878 Mthe_1598(cobN)
MHU: Mhun_0282 Mhun_0292
MEM: Memar_0040
MTH: MTH1363 MTH317 MTH318 MTH514 MTH928
MST: Msp_1110(cobN)
MSI: Msm_1117 Msm_1715
MKA: MK0663 MK0664 MK1351 MK1534
HAL: VNG1566G(cobN)
HSL: OE3230F(cobN)
HMA: rrnAC3019(cobN)
HWA: HQ2296A(cobN)
NPH: NP1092A(cobN)
HLA: Hlac_3461
HMU: Hmuk_1859
PTO: PTO0179
TON: TON_1875
MSE: Msed_2089
PIS: Pisl_0228
TNE: Tneu_0376
Taxonomy
Reference
  Authors
  Title



  Journal
  Organism
 1  [PMID:1429466]
Debussche L, Couder M, Thibaut D, Cameron B, Crouzet J, Blanche F.
Assay, purification, and characterization of cobaltochelatase, a
unique complex enzyme catalyzing cobalt insertion in
hydrogenobyrinic acid a,c-diamide during coenzyme B12 biosynthesis
in Pseudomonas denitrificans.
J. Bacteriol. 174 (1992) 7445-51.
Pseudomonas denitrificans
Reference
  Authors
  Title
  Journal
  Organism
 2  [PMID:12195810]
Warren MJ, Raux E, Schubert HL, Escalante-Semerena JC.
The biosynthesis of adenosylcobalamin (vitamin B12).
Nat. Prod. Rep. 19 (2002) 390-412.
Pseudomonas denitrificans
Other DBs ExplorEnz - The Enzyme Database: 6.6.1.2
IUBMB Enzyme Nomenclature: 6.6.1.2
ExPASy - ENZYME nomenclature database: 6.6.1.2
BRENDA, the Enzyme Database: 6.6.1.2
CAS: 81295-49-0
DBGET integrated database retrieval system