| Entry |
|
|---|
| Name |
toluene dioxygenase;
toluene 2,3-dioxygenase |
|---|
| Class |
Oxidoreductases;
Acting on paired donors, with O2 as oxidant and incorporation or
reduction of oxygen. The oxygen incorporated need not be derived
from O2;
With NADH or NADPH as one donor, and incorporation of two atoms of
oxygen into the other donor
 |
|---|
| Sysname |
toluene,NADH:oxygen oxidoreductase (1,2-hydroxylating) |
|---|
| Reaction(IUBMB) |
toluene + NADH + H+ + O2 =
(1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+ [RN:R03559] |
|---|
| Reaction(KEGG) |
R03559
 |
|---|
| Substrate |
toluene [CPD:C01455];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007] |
|---|
| Product |
(1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol [CPD:C04592];
NAD+ [CPD:C00003] |
|---|
| Comment |
A system, containing a reductase which is an iron-sulfur
flavoprotein (FAD), an iron-sulfur oxygenase, and a ferredoxin. Some
other aromatic compounds, including ethylbenzene, 4-xylene and some
halogenated toluenes, are converted into the corresponding
cis-dihydrodiols. |
|---|
| Pathway |
PATH: ec00622 Toluene and xylene degradation |
|---|
| Structures |
PDB: 3EN1 3EQQ |
|---|
Reference
Authors
Title
Journal
Organism
|
1 [PMID:16347845]
Renganathan V.
Possible Involvement of Toluene-2,3-Dioxygenase in Defluorination of
3-Fluoro-Substituted Benzenes by Toluene-Degrading Pseudomonas sp.
Strain T-12.
Appl. Environ. Microbiol. 55 (1989) 330-334.
Pseudomonas sp. |
|---|
Reference
Authors
Title
Journal
Organism
|
2 [PMID:526270]
Subramanian V, Liu TN, Yeh WK, Gibson DT.
Toluene dioxygenase: purification of an iron-sulfur protein by
affinity chromatography.
Biochem. Biophys. Res. Commun. 91 (1979) 1131-9.
Pseudomonas putida |
|---|
| Other DBs |
ExplorEnz - The Enzyme Database: 1.14.12.11
IUBMB Enzyme Nomenclature: 1.14.12.11
ExPASy - ENZYME nomenclature database: 1.14.12.11
UM-BBD (Biocatalysis/Biodegradation Database): 1.14.12.11
BRENDA, the Enzyme Database: 1.14.12.11
CAS: 120038-36-0 |
|---|
