| Entry |
|
|---|
| Name |
cholesterol 7alpha-monooxygenase;
cholesterol 7alpha-hydroxylase;
CYP7A1 |
|---|
| Class |
Oxidoreductases;
Acting on paired donors, with O2 as oxidant and incorporation or
reduction of oxygen. The oxygen incorporated need not be derived
from O2;
With NADH or NADPH as one donor, and incorporation of one atom of
oxygen into the other donor
 |
|---|
| Sysname |
cholesterol,NADPH:oxygen oxidoreductase (7alpha-hydroxylating) |
|---|
| Reaction(IUBMB) |
cholesterol + NADPH + H+ + O2 = 7alpha-hydroxycholesterol + NADP+ +
H2O [RN:R01463] |
|---|
| Reaction(KEGG) |
R01463 > R07372;
(other) R08961
 |
|---|
| Substrate |
cholesterol [CPD:C00187];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007] |
|---|
| Product |
7alpha-hydroxycholesterol [CPD:C03594];
NADP+ [CPD:C00006];
H2O [CPD:C00001] |
|---|
| Cofactor |
Heme [CPD:C00032] |
|---|
| Comment |
A heme-thiolate protein (P-450). |
|---|
| Pathway |
PATH: ec00120 Primary bile acid biosynthesis
PATH: ec00140 Steroid hormone biosynthesis
PATH: ec01100 Metabolic pathways |
|---|
| Orthology |
KO: K00489 cytochrome P450, family 7, subfamily A (cholesterol
7alpha-monooxygenase) |
|---|
| Genes |
HSA: 1581(CYP7A1)
PTR: 464191(CYP7A1)
MCC: 698127
MMU: 13122(Cyp7a1)
RNO: 25428(Cyp7a1)
CFA: 486962
BTA: 510507
SSC: 448985(CYP7A1)
ECB: 100052888
MDO: 100022261(CYP7A1)
OAA: 100079392
GGA: 414834(CYP7A1)
TGU: 100225999
DRE: 394148
 |
|---|
| Structures |
PDB: 3DAX |
|---|
Reference
Authors
Title
Journal
Organism
|
1 [PMID:4147676]
Boyd GS, Grimwade AM, Lawson ME.
Studies on rat-liver microsomal cholesterol 7alpha-hydroxylase.
Eur. J. Biochem. 37 (1973) 334-40.
Rattus norvegicus [GN:rno] |
|---|
Reference
Authors
Title
Journal
Organism
|
2 [PMID:4397276]
Mitton JR, Scholan NA, Boyd GS.
The oxidation of cholesterol in rat liver sub-cellular particles.
The cholesterol-7-alpha-Hydroxylase enzyme system.
Eur. J. Biochem. 20 (1971) 569-79.
Rattus norvegicus [GN:rno] |
|---|
Reference
Authors
Title
Journal
Organism
|
3 [PMID:3584134]
Ogishima T, Deguchi S, Okuda K.
Purification and characterization of cholesterol 7 alpha-hydroxylase
from rat liver microsomes.
J. Biol. Chem. 262 (1987) 7646-50.
Rattus norvegicus [GN:rno] |
|---|
| Other DBs |
ExplorEnz - The Enzyme Database: 1.14.13.17
IUBMB Enzyme Nomenclature: 1.14.13.17
ExPASy - ENZYME nomenclature database: 1.14.13.17
BRENDA, the Enzyme Database: 1.14.13.17
CAS: 9037-53-0 |
|---|
