| Entry |
|
|---|
| Name |
isoflavone 3'-hydroxylase;
isoflavone 3'-monooxygenase |
|---|
| Class |
Oxidoreductases;
Acting on paired donors, with O2 as oxidant and incorporation or
reduction of oxygen. The oxygen incorporated need not be derived
from O2;
With NADH or NADPH as one donor, and incorporation of one atom of
oxygen into the other donor
 |
|---|
| Sysname |
formononetin,NADPH:oxygen oxidoreductase (3'-hydroxylating) |
|---|
| Reaction(IUBMB) |
formononetin + NADPH + H+ + O2 = calycosin + NADP+ + H2O [RN:R03006] |
|---|
| Reaction(KEGG) |
R03006;
(other) R07776
 |
|---|
| Substrate |
formononetin [CPD:C00858];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007] |
|---|
| Product |
calycosin [CPD:C01562];
NADP+ [CPD:C00006];
H2O [CPD:C00001] |
|---|
| Cofactor |
Heme [CPD:C00032] |
|---|
| Comment |
A heme-thiolate protein (P-450). Also acts on biochanin A and other
isoflavones with a 4'-methoxy group. Involved in the biosynthesis of
the pterocarpin phytoalexins medicarpin and maackiain. |
|---|
| Pathway |
PATH: ec00943 Isoflavonoid biosynthesis
PATH: ec01061 Biosynthesis of phenylpropanoids |
|---|
Reference
Authors
Title
Journal
|
1
Hinderer, W., Flentje, U. and Barz, W.
Microsomal isoflavone 2'-hydroxylases and 3'-hydroxylases from
chickpea (Cicer arietinum L) cell-suspensions induced for
pterocarpan phytoalexin formation.
FEBS Lett. 214 (1987) 101-106. |
|---|
| Other DBs |
ExplorEnz - The Enzyme Database: 1.14.13.52
IUBMB Enzyme Nomenclature: 1.14.13.52
ExPASy - ENZYME nomenclature database: 1.14.13.52
BRENDA, the Enzyme Database: 1.14.13.52
CAS: 110183-50-1 |
|---|
