| Entry |
|
|---|
| Name |
4'-methoxyisoflavone 2'-hydroxylase;
isoflavone 2'-monooxygenase (ambiguous);
isoflavone 2'-hydroxylase (ambiguous) |
|---|
| Class |
Oxidoreductases;
Acting on paired donors, with O2 as oxidant and incorporation or
reduction of oxygen. The oxygen incorporated need not be derived
from O2;
With NADH or NADPH as one donor, and incorporation of one atom of
oxygen into the other donor
 |
|---|
| Sysname |
formononetin,NADPH:oxygen oxidoreductase (2'-hydroxylating) |
|---|
| Reaction(IUBMB) |
formononetin + NADPH + H+ + O2 = 2'-hydroxyformononetin + NADP+ +
H2O |
|---|
| Substrate |
formononetin [CPD:C00858];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007] |
|---|
| Product |
2'-hydroxyformononetin [CPD:C02920];
NADP+ [CPD:C00006];
H2O [CPD:C00001] |
|---|
| Cofactor |
Heme [CPD:C00032] |
|---|
| Comment |
A heme-thiolate protein (P-450). Acts on isoflavones with a
4'-methoxy group, such as formononetin and biochanin A. Involved in
the biosynthesis of the pterocarpin phytoalexins medicarpin and
maackiain. EC 1.14.13.89, isoflavone 2'-hydroxylase, is less
specific and acts on other isoflavones as well as
4'-methoxyisoflavones. |
|---|
| Pathway |
PATH: ec01061 Biosynthesis of phenylpropanoids |
|---|
Reference
Authors
Title
Journal
|
1
Hinderer, W., Flentje, U. and Barz, W.
Microsomal isoflavone 2'-hydroxylases and 3'-hydroxylases from
chickpea (Cicer arietinum L) cell-suspensions induced for
pterocarpan phytoalexin formation.
FEBS Lett. 214 (1987) 101-106. |
|---|
| Other DBs |
ExplorEnz - The Enzyme Database: 1.14.13.53
IUBMB Enzyme Nomenclature: 1.14.13.53
ExPASy - ENZYME nomenclature database: 1.14.13.53
BRENDA, the Enzyme Database: 1.14.13.53
CAS: 110183-49-8 |
|---|
