| Entry |
|
|---|
| Name |
camphor 5-monooxygenase;
camphor 5-exo-methylene hydroxylase;
2-bornanone 5-exo-hydroxylase;
bornanone 5-exo-hydroxylase;
camphor 5-exo-hydroxylase;
camphor 5-exohydroxylase;
camphor hydroxylase;
d-camphor monooxygenase;
methylene hydroxylase;
methylene monooxygenase;
D-camphor-exo-hydroxylase;
camphor methylene hydroxylase |
|---|
| Class |
Oxidoreductases;
Acting on paired donors, with O2 as oxidant and incorporation or
reduction of oxygen. The oxygen incorporated need not be derived
from O2;
With reduced iron-sulfur protein as one donor, and incorporation of
one atom of oxygen into the other donor
 |
|---|
| Sysname |
(+)-camphor,reduced putidaredoxin:oxygen oxidoreductase
(5-hydroxylating) |
|---|
| Reaction(IUBMB) |
(+)-camphor + putidaredoxin + O2 = (+)-exo-5-hydroxycamphor +
oxidized putidaredoxin + H2O [RN:R03818] |
|---|
| Reaction(KEGG) |
R03818
 |
|---|
| Substrate |
(+)-camphor [CPD:C00808];
putidaredoxin [CPD:C02069];
O2 [CPD:C00007] |
|---|
| Product |
(+)-exo-5-hydroxycamphor [CPD:C03448];
oxidized putidaredoxin [CPD:C03302];
H2O [CPD:C00001] |
|---|
| Cofactor |
Heme [CPD:C00032] |
|---|
| Comment |
A heme-thiolate protein (P-450). Also acts on (-)-camphor and
1,2-campholide, forming 5-exo-hydroxy-1,2-campholide. |
|---|
| Structures |
PDB: 1AKD 1C8J 1CP4 1GEB 1GEK 1GEM 1GJM 1IWI 1IWJ 1IWK
1J51 1K2O 1LWL 1MPW 1NOO 1O76 1P2Y 1P7R 1PHA 1PHB
1PHC 1PHD 1PHE 1PHF 1PHG 1QMQ 1RE9 1RF9 1T85 1T86
1T87 1T88 1YRC 1YRD 2A1M 2A1N 2A1O 2CP4 2CPP 2FE6
2FER 2FEU 2FRZ 2GQX 2GR6 2H7Q 2H7R 2H7S 2QBL 2QBM
2QBN 2QBO 2Z97 2ZAW 2ZAX 2ZUH 2ZUI 2ZUJ 2ZWT 2ZWU
3CP4 3CPP 3FWF 3FWG 3FWI 3FWJ 4CP4 4CPP 5CP4 5CPP
6CP4 6CPP 7CPP 8CPP |
|---|
Reference
Authors
Title
Journal
Organism
|
1 [PMID:4378858]
Hedegaard J, Gunsalus IC.
Mixed function oxidation. IV. An induced methylene hydroxylase in
camphor oxidation.
J. Biol. Chem. 240 (1965) 4038-43.
Pseudomonas putida |
|---|
Reference
Authors
Title
Journal
Organism
|
2 [PMID:4341491]
Tyson CA, Lipscomb JD, Gunsalus IC.
The role of putidaredoxin and P450 cam in methylene hydroxylation.
J. Biol. Chem. 247 (1972) 5777-84.
Pseudomonas putida |
|---|
| Other DBs |
ExplorEnz - The Enzyme Database: 1.14.15.1
IUBMB Enzyme Nomenclature: 1.14.15.1
ExPASy - ENZYME nomenclature database: 1.14.15.1
UM-BBD (Biocatalysis/Biodegradation Database): 1.14.15.1
BRENDA, the Enzyme Database: 1.14.15.1
CAS: 9030-82-4 |
|---|
