| Entry |
|
|---|
| Name |
nicotine dehydrogenase;
nicotine oxidase;
D-nicotine oxidase;
nicotine:(acceptor) 6-oxidoreductase (hydroxylating);
L-nicotine oxidase |
|---|
| Class |
Oxidoreductases;
Acting on the CH-NH group of donors;
With other acceptors
 |
|---|
| Sysname |
nicotine:acceptor 6-oxidoreductase (hydroxylating) |
|---|
| Reaction(IUBMB) |
(S)-nicotine + acceptor + H2O = (S)-6-hydroxynicotine + reduced
acceptor [RN:R02860] |
|---|
| Reaction(KEGG) |
R02860 > R07946
 |
|---|
| Substrate |
(S)-nicotine [CPD:C00745];
acceptor [CPD:C00028];
H2O [CPD:C00001] |
|---|
| Product |
(S)-6-hydroxynicotine [CPD:C01056];
reduced acceptor [CPD:C00030] |
|---|
| Cofactor |
Metal [CPD:C00050];
FMN [CPD:C00061] |
|---|
| Comment |
A metalloprotein (FMN). The enzyme can act on both the naturally
found (S)-enantiomer and the synthetic (R)-enantiomer of nicotine,
with retention of configuration in both cases [4]. |
|---|
| Pathway |
PATH: ec00760 Nicotinate and nicotinamide metabolism |
|---|
Reference
Authors
Title
Journal
Organism
|
1 [PMID:13475371]
BEHRMAN EJ, STANIER RY.
The bacterial oxidation of nicotinic acid.
J. Biol. Chem. 228 (1957) 923-45.
Pseudomonas fluorescens |
|---|
Reference
Authors
Title
Journal
Organism
|
2 [PMID:5849820]
Decker K, Bleeg H.
Induction and purification of stereospecific nicotine oxidizing
enzymes from Arthrobacter oxidans.
Biochim. Biophys. Acta. 105 (1965) 313-24.
Arthrobacter oxidans |
|---|
Reference
Authors
Title
Journal
Organism
|
3 [PMID:4962139]
Hochstein LI, Dalton BP.
The purification and properties of nicotine oxidase.
Biochim. Biophys. Acta. 139 (1967) 56-68.
Arthrobacter oxidans |
|---|
Reference
Authors
Title
Journal
Organism
|
4 [PMID:13610912]
HOCHSTEIN LI, RITTENBERG SC.
The bacterial oxidation of nicotine. II. The isolation of the first
oxidative product and its identification as (1)-6-hydroxynicotine.
J. Biol. Chem. 234 (1959) 156-60.
Corynebacterium nicotinovorum |
|---|
| Other DBs |
ExplorEnz - The Enzyme Database: 1.5.99.4
IUBMB Enzyme Nomenclature: 1.5.99.4
ExPASy - ENZYME nomenclature database: 1.5.99.4
UM-BBD (Biocatalysis/Biodegradation Database): 1.5.99.4
BRENDA, the Enzyme Database: 1.5.99.4
CAS: 37256-31-8 |
|---|
