KEGG   ENZYME: 4.2.1.84Help
Entry
EC 4.2.1.84                 Enzyme                                 
Name nitrile hydratase;
nitrilase (ambiguous);
3-cyanopyridine hydratase;
NHase;
L-NHase;
H-NHase;
acrylonitrile hydratase;
aliphatic nitrile hydratase;
nitrile hydro-lyase
Class Lyases;
Carbon-oxygen lyases;
Hydro-lyases
BRITE hierarchy
Sysname aliphatic-amide hydro-lyase (nitrile-forming)
Reaction(IUBMB) an aliphatic amide = a nitrile + H2O [RN:R02826]
Reaction(KEGG) R02826 > R04020 R05379 R07854;
(other) R02828 R05596 R07780
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Substrate aliphatic amide [CPD:C02244]
Product nitrile [CPD:C00726];
H2O [CPD:C00001]
Cofactor Iron [CPD:C00023];
PQQ [CPD:C00113];
Cobalt ion [CPD:C00175]
Inhibitor Semicarbazide [CPD:C02077];
Phenylhydrazine [CPD:C02304];
Isobutyronitrile [CPD:C02420]
Comment Acts on short-chain aliphatic nitriles, converting them into the
corresponding amides. Does not act on these amides or on aromatic
nitriles. cf. EC 3.5.5.1 nitrilase.
Pathway PATH: ec00380  Tryptophan metabolism
PATH: ec00460  Cyanoamino acid metabolism
PATH: ec00627  1,4-Dichlorobenzene degradation
PATH: ec00632  Benzoate degradation via CoA ligation
PATH: ec00643  Styrene degradation
Orthology KO: K01721  nitrile hydratase
Genes KPE: KPK_2672(nthA) KPK_2673(nthB)
PPF: Pput_2728 Pput_2729
PFS: PFLU3209 PFLU3210
ACI: ACIAD1615(nthB) ACIAD1616(nthA)
BCN: Bcen_4082 Bcen_4083
BCH: Bcen2424_4283 Bcen2424_4284
BCM: Bcenmc03_3233 Bcenmc03_3234
BAM: Bamb_6543 Bamb_6544
BAC: BamMC406_6257 BamMC406_6258
BPY: Bphyt_5089 Bphyt_5090 Bphyt_7181 Bphyt_7182
BPT: Bpet1415(nthB) Bpet1416(nthA)
VAP: Vapar_1665 Vapar_1666 Vapar_2111 Vapar_2112
MLO: mll1423
SME: SMc01423(nthB) SMc01424(nthA)
SMD: Smed_2000 Smed_2001
ARA: Arad_2889(nthB) Arad_2890(nthA)
RET: RHE_CH02782(nthB) RHE_CH02783(nthA)
REC: RHECIAT_CH0002923(nthB) RHECIAT_CH0002924(nthA)
RLE: RL3238(nhb2) RL3239(nha1) pRL110124 pRL110125
RLT: Rleg2_2525 Rleg2_2526 Rleg2_4710 Rleg2_4711
RLG: Rleg_1540 Rleg_2784 Rleg_2785 Rleg_6980 Rleg_6981
RHI: NGR_c20110(nthB) NGR_c20120(nthA)
BJA: bll4497(nthB)
BRA: BRADO3845(nthA) BRADO3846(nthB)
BBT: BBta_4084(nthB) BBta_4085(nthA)
RPA: RPA2805(nthA) RPA2806(nthB)
RPB: RPB_2704 RPB_2705
RPC: RPC_2748 RPC_2749
RPD: RPD_2746 RPD_2747
RPT: Rpal_3145 Rpal_3146
AZC: AZC_3797 AZC_3798
MRD: Mrad2831_0904 Mrad2831_0905
MET: M446_6960 M446_6961
MPO: Mpop_3787
MNO: Mnod_7674 Mnod_7675
MSL: Msil_3459 Msil_3460
SIL: SPO1314(nthA) SPO1315(nthB)
SIT: TM1040_1971 TM1040_1972
RDE: RD1_1908(nthA) RD1_1909(nthB)
DSH: Dshi_1849(nthA)
ACR: Acry_1077
MSM: MSMEG_0377 MSMEG_0378(nthA)
MVA: Mvan_0647 Mvan_0648 Mvan_5825 Mvan_5826
MGI: Mflv_1010 Mflv_1011
MJL: Mjls_5637
RHA: RHA1_ro00360(nthA) RHA1_ro00361(nthB)
RER: RER_59240(nha1) RER_59250(nha2)
AAU: AAur_pTC20145(nthB) AAur_pTC20146(nthA) AAur_pTC20147(nthB)
RXY: Rxyl_2315
SYP: SYNPCC7002_D0007(nthA) SYNPCC7002_D0008(nthB)
AMR: AM1_1194(nthA) AM1_1195(nthB)
Taxonomy
Structures PDB: 1AHJ  1IRE  1UGP  1UGQ  1UGR  1UGS  1V29  2AHJ  2CYZ  2CZ0  
     2CZ1  2CZ6  2CZ7  2D0Q  2DPP  2QDY  2ZCF  2ZPB  2ZPE  2ZPF  
     2ZPG  2ZPH  2ZPI  3HHT  
Reference
  Authors
  Title


  Journal
 1
Asano, Y., Fujishiro, K., Tani, Y. and Yamada, H.
Microbial degradation of nitrile compounds. 5. Aliphatic nitrile
hydratase from Arthrobacter sp J-1. Purification and
characterization.
Agric. Biol. Chem. 46 (1982) 1165-1174.
Other DBs ExplorEnz - The Enzyme Database: 4.2.1.84
IUBMB Enzyme Nomenclature: 4.2.1.84
ExPASy - ENZYME nomenclature database: 4.2.1.84
UM-BBD (Biocatalysis/Biodegradation Database): 4.2.1.84
BRENDA, the Enzyme Database: 4.2.1.84
CAS: 82391-37-5
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