PMID:
     12429849
Authors:
     Scherl A, Coute Y, Deon C, Calle A, Kindbeiter K, Sanchez JC,
     Greco A, Hochstrasser D, Diaz JJ.
Title:
     Functional proteomic analysis of human nucleolus.
Journal:
     Mol Biol Cell. 2002 Nov;13(11):4100-9.
Abstract:
     The notion of a "plurifunctional" nucleolus is now well established. However,
     molecular mechanisms underlying the biological processes occurring within this
     nuclear domain remain only partially understood. As a first step in elucidating
     these mechanisms we have carried out a proteomic analysis to draw up a list of
     proteins present within nucleoli of HeLa cells. This analysis allowed the
     identification of 213 different nucleolar proteins. This catalog complements that
     of the 271 proteins obtained recently by others, giving a total of approximately 
     350 different nucleolar proteins. Functional classification of these proteins
     allowed outlining several biological processes taking place within nucleoli.
     Bioinformatic analyses permitted the assignment of hypothetical functions for 43 
     proteins for which no functional information is available. Notably, a role in
     ribosome biogenesis was proposed for 31 proteins. More generally, this functional
     classification reinforces the plurifunctional nature of nucleoli and provides
     convincing evidence that nucleoli may play a central role in the control of gene 
     expression. Finally, this analysis supports the recent demonstration of a
     coupling of transcription and translation in higher eukaryotes.

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