Database: PubMedEntry: 1570292
Original site: 1570292
Anderson JA, Huprikar SS, Kochian LV, Lucas WJ, Gaber RF.
Functional expression of a probable Arabidopsis thaliana potassium channel in
Proc Natl Acad Sci U S A. 1992 May 1;89(9):3736-40.
We report the isolation of a cDNA (KAT1) from Arabidopsis thaliana that encodes a
probable K+ channel. KAT1 was cloned by its ability to suppress a K+
transport-defective phenotype in mutant Saccharomyces cerevisiae cells. This
suppression is sensitive to known K+ channel blockers, including
tetraethylammonium and Ba2+ ions. The KAT1 cDNA contains an open reading frame
capable of encoding a 78-kDa protein that shares structural features found in the
Shaker superfamily of K+ channels. These include a cluster of six putative
membrane-spanning helices (S1-S6) at the amino terminus of the protein, a
presumed voltage-sensing region containing Arg/Lys-Xaa-Xaa-Arg/Lys repeats within
S4, and the highly conserved pore-forming region (known as H5 or SS1-SS2). Our
results suggest that the structural motif for K+ channels has been conserved
between plants and animals.
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