Database: PubMedEntry: 8740180
Original site: 8740180
Sanchez JC, Golaz O, Frutiger S, Schaller D, Appel RD, Bairoch A,
Hughes GJ, Hochstrasser DF.
The yeast SWISS-2DPAGE database.
Electrophoresis. 1996 Mar;17(3):556-65.
The systematic sequencing of the yeast genome will soon be completed. A new
challenge has been launched by the EUROFAN (European Functional Analysis) project
whose goal is to elucidate the physiological and biochemical function of newly
discovered open reading frames (ORF) from yeast. One of the approaches is to use
protein-based technologies such as two-dimensional gel electrophoresis and
protein identification in order to establish a yeast reference map. Modified
protein patterns can be compared to the reference map which hopefully will help
identify changes related, for example, to growth processes or developmental
events. This paper describes the yeast SWISS-2DPAGE database in which charge
separation was obtained using immobilized pH gradient (IPG). Proteins identified
by gel comparison, amino acid composition analysis and/or microsequencing are
recorded and described in an accessible uniform format. We have identified more
than one hundred polypeptides, several of which were newly mapped. In addition,
the yeast SWISS-2DPAGE database can be freely accessed through the World Wide Web
(WWW) network on the ExPASy molecular biology server.
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