PMID:
     9150929
Authors:
     Yan JX, Tonella L, Sanchez JC, Wilkins MR, Packer NH, Gooley AA,
     Hochstrasser DF, Williams KL.
Title:
     The Dictyostelium discoideum proteome--the SWISS-2DPAGE database of the
     multicellular aggregate (slug).
Journal:
     Electrophoresis. 1997 Mar-Apr;18(3-4):491-7.
Abstract:
     The cellular slime mold Dictyostelium discoideum is a eukaryotic microorganism
     which has developmental life stages attractive to the cell and molecular
     biologist. By displaying the two-dimensional polyacrylamide gel electrophoresis
     (2-D PAGE) protein map of different developmental stages, the key molecules can
     be identified and characterised, allowing a detailed understanding of the D.
     discoideum proteome. Here we describe the preparation of reference gel of the D. 
     discoideum multicellular aggregate, the slug. Proteins were separated by 2-D PAGE
     with immobilised pH gradients (pH 3.5-10) in the first dimension and sodium
     dodecyl sulfate (SDS)-PAGE in the second dimension. Micropreparative gels were
     electroblotted onto polyvinylidene difluoride (PVDF) membranes and 150 spots were
     visualised by amido black staining. Protein spots were excised and 31 were
     putatively identified by matching their amino acid composition, estimated
     isoelectric point (pI) and molecular weight (M(r)) against the SWISS-PROT
     database with the ExPASy AAcompID tool (http://
     expasy.hcuge.ch/ch2d/aacompi.html). A total of 25 proteins were identified by
     matching against database entries for D. discoideum, and another six by
     cross-species matching against database entries for Saccharomyces cerevisiae
     proteins. This map will be available in the SWISS-2DPAGE database.

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