PMID:
     9738472
Authors:
     Partiseti M, Collura V, Agnel M, Culouscou JM, Graham D.
Title:
     Cloning and characterization of a novel human inwardly rectifying potassium
     channel predominantly expressed in small intestine.
Journal:
     FEBS Lett. 1998 Aug 28;434(1-2):171-6.
Abstract:
     A new member of the two transmembrane domain potassium (K+) channel family was
     identified and isolated from a human brain cDNA library. The cDNA clone contains 
     an open reading frame which encodes a 360 amino acid sequence with a
     characteristic P domain flanked by two hydrophobic regions representing the
     membrane spanning segments. The closest homologue of this gene product is the
     inwardly rectifying potassium channel subunit, Kir1.2 (identity approximately
     42%). Northern blot analysis of human tissues with a selective cDNA probe for
     this new K+ subunit showed a single major transcript of 3.4 kb predominantly
     expressed at high levels in small intestine, with lower levels in stomach, kidney
     and brain. The main regions of expression in the central nervous system were
     medulla, hippocampus and corpus callosum. cRNA-injected oocytes and transiently
     transfected HEK293 cells expressed a K+ conductance which displays an inward
     rectification. This conductance is blocked by cesium and barium but is
     insensitive to tolbutamide and diazoxide even upon co-transfection of this novel 
     subunit with the plasmid encoding the sulfonylurea receptor SUR1. Taken together,
     these results demonstrate that we have isolated and characterized a novel K+
     channel subunit belonging to the inwardly rectifying K+ (Kir) channel family to
     which, upon homology classification, we have given the nomenclature Kir7.1.

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