Database: PubMedEntry: 9738472
Original site: 9738472
Partiseti M, Collura V, Agnel M, Culouscou JM, Graham D.
Cloning and characterization of a novel human inwardly rectifying potassium
channel predominantly expressed in small intestine.
FEBS Lett. 1998 Aug 28;434(1-2):171-6.
A new member of the two transmembrane domain potassium (K+) channel family was
identified and isolated from a human brain cDNA library. The cDNA clone contains
an open reading frame which encodes a 360 amino acid sequence with a
characteristic P domain flanked by two hydrophobic regions representing the
membrane spanning segments. The closest homologue of this gene product is the
inwardly rectifying potassium channel subunit, Kir1.2 (identity approximately
42%). Northern blot analysis of human tissues with a selective cDNA probe for
this new K+ subunit showed a single major transcript of 3.4 kb predominantly
expressed at high levels in small intestine, with lower levels in stomach, kidney
and brain. The main regions of expression in the central nervous system were
medulla, hippocampus and corpus callosum. cRNA-injected oocytes and transiently
transfected HEK293 cells expressed a K+ conductance which displays an inward
rectification. This conductance is blocked by cesium and barium but is
insensitive to tolbutamide and diazoxide even upon co-transfection of this novel
subunit with the plasmid encoding the sulfonylurea receptor SUR1. Taken together,
these results demonstrate that we have isolated and characterized a novel K+
channel subunit belonging to the inwardly rectifying K+ (Kir) channel family to
which, upon homology classification, we have given the nomenclature Kir7.1.
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