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Entry
mmu03030                    Pathway                                

Name
DNA replication - Mus musculus (mouse)
Description
A complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (DnaB or MCM complex) precedes the DNA synthetic machinery and unwinds the duplex parental DNA in cooperation with the SSB or RPA. On the leading strand, replication occurs continuously in a 5 to 3 direction, whereas on the lagging strand, DNA replication occurs discontinuously by synthesis and joining of short Okazaki fragments. In prokaryotes, the leading strand replication apparatus consists of a DNA polymerase (pol III core), a sliding clamp (beta), and a clamp loader (gamma delta complex). The DNA primase (DnaG) is needed to form RNA primers. Normally, during replication of the lagging-strand DNA template, an RNA primer is removed either by an RNase H or by the 5 to 3 exonuclease activity of DNA pol I, and the DNA ligase joins the Okazaki fragments. In eukaryotes, three DNA polymerases (alpha, delta, and epsilon) have been identified. DNA primase forms a permanent complex with DNA polymerase alpha. PCNA and RFC function as a clamp and a clamp loader. FEN 1 and RNase H1 remove the RNA from the Okazaki fragments and DNA ligase I joins the DNA.
Class
Genetic Information Processing; Replication and repair
BRITE hierarchy
Pathway map
DNA replication
mmu03030

All organismsOrtholog table
Module
DNA polymerase alpha / primase complex [PATH:mmu03030]
DNA polymerase delta complex [PATH:mmu03030]
DNA polymerase epsilon complex [PATH:mmu03030]
MCM complex [PATH:mmu03030]
RPA complex [PATH:mmu03030]
RF-C complex [PATH:mmu03030]
Organism
Mus musculus (mouse) [GN:mmu]
Gene
381760  
Ssbp1; single-stranded DNA binding protein 1 [KO:K03111]
19819  
Rnaseh1; ribonuclease H1 [KO:K03469] [EC:3.1.26.4]
68275  
Rpa1; replication protein A1 [KO:K07466]
18538  
Pcna; proliferating cell nuclear antigen [KO:K04802]
327762  
Dna2; DNA replication helicase/nuclease 2 [KO:K10742] [EC:3.6.4.12]
14156  
Fen1; flap structure specific endonuclease 1 [KO:K04799] [EC:3.-.-.-]
16881  
Lig1; ligase I, DNA, ATP-dependent [KO:K10747] [EC:6.5.1.7 6.5.1.6 6.5.1.1]
18968  
Pola1; polymerase (DNA directed), alpha 1 [KO:K02320] [EC:2.7.7.7]
18969  
Pola2; polymerase (DNA directed), alpha 2 [KO:K02321]
19075  
Prim1; DNA primase, p49 subunit [KO:K02684] [EC:2.7.7.-]
19076  
Prim2; DNA primase, p58 subunit [KO:K02685] [EC:2.7.7.-]
18971  
Pold1; polymerase (DNA directed), delta 1, catalytic subunit [KO:K02327] [EC:2.7.7.7]
18972  
Pold2; polymerase (DNA directed), delta 2, regulatory subunit [KO:K02328]
67967  
Pold3; polymerase (DNA-directed), delta 3, accessory subunit [KO:K03504]
69745  
Pold4; polymerase (DNA-directed), delta 4 [KO:K03505]
18973  
Pole; polymerase (DNA directed), epsilon [KO:K02324] [EC:2.7.7.7]
18974  
Pole2; polymerase (DNA directed), epsilon 2 (p59 subunit) [KO:K02325] [EC:2.7.7.7]
59001  
Pole3; polymerase (DNA directed), epsilon 3 (p17 subunit) [KO:K02326] [EC:2.7.7.7]
66979  
Pole4; polymerase (DNA-directed), epsilon 4 (p12 subunit) [KO:K03506] [EC:2.7.7.7]
17216  
Mcm2; minichromosome maintenance complex component 2 [KO:K02540] [EC:3.6.4.12]
17215  
Mcm3; minichromosome maintenance complex component 3 [KO:K02541] [EC:3.6.4.12]
17217  
Mcm4; minichromosome maintenance complex component 4 [KO:K02212] [EC:3.6.4.12]
17218  
Mcm5; minichromosome maintenance complex component 5 [KO:K02209] [EC:3.6.4.12]
17219  
Mcm6; minichromosome maintenance complex component 6 [KO:K02542] [EC:3.6.4.12]
17220  
Mcm7; minichromosome maintenance complex component 7 [KO:K02210] [EC:3.6.4.12]
19891  
Rpa2; replication protein A2 [KO:K10739]
68240  
Rpa3; replication protein A3 [KO:K10740]
19687  
Rfc1; replication factor C (activator 1) 1 [KO:K10754]
106344  
Rfc4; replication factor C (activator 1) 4 [KO:K10755]
19718  
Rfc2; replication factor C (activator 1) 2 [KO:K10755]
72151  
Rfc5; replication factor C (activator 1) 5 [KO:K10756]
69263  
Rfc3; replication factor C (activator 1) 3 [KO:K10756]
69724  
Rnaseh2a; ribonuclease H2, large subunit [KO:K10743] [EC:3.1.26.4]
67153  
Rnaseh2b; ribonuclease H2, subunit B [KO:K10744]
68209  
Rnaseh2c; ribonuclease H2, subunit C [KO:K10745]
Reference
PMID:8087839
  Authors
Stillman B.
  Title
Smart machines at the DNA replication fork.
  Journal
Cell 78:725-8 (1994)
DOI:10.1016/S0092-8674(94)90362-X
Reference
PMID:9759502
  Authors
Waga S, Stillman B.
  Title
The DNA replication fork in eukaryotic cells.
  Journal
Annu Rev Biochem 67:721-51 (1998)
DOI:10.1146/annurev.biochem.67.1.721
KO pathway
 

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