Meiosis - yeast - Ashbya gossypii (Eremothecium gossypii)

During meiosis, a single round of DNA replication is followed by two rounds of chromosome segregation, called meiosis I and meiosis II. At meiosis I, one pair of sister chromatids recombines with, then segregates from, its homologous pair, while the sister chromatids segregate from each other at meoisis II. In yeast, the switch to enter growth phase or meiosis phase is controlled by nutritional signals. At the onset of meiosis, nutritional signaling pathways converge on transcriptional regulation of Ime1. Ime1 is a transcriptional activator to promote expression of early meiosis-specific genes required for pre-meiotic DNA replication and meiotic recombination. Two of the early meiosis-specific genes, a transcriptional activator, Ndt80, and a CDK2 homolog, Ime2, are required for the transcription of middle meiosis-specific genes, which includes B-type cyclin genes and genes involved in spore wall formation. Polo-like kinase Cdc5, whose expression is activated by Ndt80, is required for the critical step in meiotic progression. Cdc5 regulates sister-kinetochore co-orientation by promoting the localization of Mam1 and Lrs4, components of the co-orientation complex, to kinetochores. Cdc5 also phosphorylates the meiotic cohesin subunit Rec8, which is essential for its cleavage and removal by separase. Moreover, Cdc5 promotes the release of protein phosphatase Cdc14 from the nuclezus, which is required for anaphase I spindle disassembly and exit from meiosis I. The centromeric protein Sgo1 is a key regulator of meiotic chromosome segregation. Sgo1 with PP2A may protect pericentromeric but not arm cohesion of sister chromatids during meiosis I. Sgo1 then disappears at meiosis II to allow sister chromatids to separate.

Cellular Processes; Cell growth and death

Ashbya gossypii (Eremothecium gossypii) [GN:ago]

PMID:12730290

Honigberg SM, Purnapatre K

Signal pathway integration in the switch from the mitotic cell cycle to meiosis in yeast.

J Cell Sci 116:2137-47 (2003)

PMID:12722950

Kassir Y, Adir N, Boger-Nadjar E, Raviv NG, Rubin-Bejerano I, Sagee S, Shenhar G

Transcriptional regulation of meiosis in budding yeast.

Int Rev Cytol 224:111-71 (2003)

PMID:15258924

Honigberg SM

Ime2p and Cdc28p: co-pilots driving meiotic development.

J Cell Biochem 92:1025-33 (2004)

PMID:16546077

Hochwagen A, Amon A

Checking your breaks: surveillance mechanisms of meiotic recombination.

Curr Biol 16:R217-28 (2006)

PMID:18832063

Hollingsworth NM

Deconstructing meiosis one kinase at a time: polo pushes past pachytene.

Genes Dev 22:2596-600 (2008)

PMID:18832066

Sourirajan A, Lichten M

Polo-like kinase Cdc5 drives exit from pachytene during budding yeast meiosis.

Genes Dev 22:2627-32 (2008)

PMID:10973068

Roeder GS, Bailis JM

The pachytene checkpoint.

Trends Genet 16:395-403 (2000)

PMID:15173822

Barr FA, Sillje HH, Nigg EA

Polo-like kinases and the orchestration of cell division.

Nat Rev Mol Cell Biol 5:429-40 (2004)

PMID:12963824

Lee BH, Amon A

Polo kinase--meiotic cell cycle coordinator.

Cell Cycle 2:400-2 (2003)

PMID:12737807

Buonomo SB, Rabitsch KP, Fuchs J, Gruber S, Sullivan M, Uhlmann F, Petronczki M, Toth A, Nasmyth K

Division of the nucleolus and its release of CDC14 during anaphase of meiosis I depends on separase, SPO12, and SLK19.

Dev Cell 4:727-39 (2003)

PMID:15797379

Oelschlaegel T, Schwickart M, Matos J, Bogdanova A, Camasses A, Havlis J, Shevchenko A, Zachariae W

The yeast APC/C subunit Mnd2 prevents premature sister chromatid separation triggered by the meiosis-specific APC/C-Ama1.

Cell 120:773-88 (2005)

PMID:16759372

Dai W, Wang X

The Yin and Yang of centromeric cohesion of sister chromatids: mitotic kinases meet protein phosphatase 2A.

Cell Div 1:9 (2006)

PMID:19299562

Clift D, Bizzari F, Marston AL

Shugoshin prevents cohesin cleavage by PP2A(Cdc55)-dependent inhibition of separase.

Genes Dev 23:766-80 (2009)

PMID:18598214

Pesin JA, Orr-Weaver TL

Regulation of APC/C activators in mitosis and meiosis.

Annu Rev Cell Dev Biol 24:475-99 (2008)

PMID:18946082

Diamond AE, Park JS, Inoue I, Tachikawa H, Neiman AM

The anaphase promoting complex targeting subunit Ama1 links meiotic exit to cytokinesis during sporulation in Saccharomyces cerevisiae.

Mol Biol Cell 20:134-45 (2009)