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Entry
ecf02060                    Pathway                                

Name
Phosphotransferase system (PTS) - Escherichia coli O157:H7 EC4115 (EHEC)
Description
The phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS) is a major mechanism used by bacteria for uptake of carbohydrates, particularly hexoses, hexitols, and disaccharides, where the source of energy is from PEP. The PTS consists of two general components, enzyme I (EI) and histidine phosphocarrier protein (HPr), and of membrane-bound sugar specific permeases (enzymes II). Each enzyme II (EII) complex consists of one or two hydrophobic integral membrane domains (domains C and D) and two hydrophilic domains (domains A and B). EII complexes may exist as distinct proteins or as a single multidomain protein. The PTS catalyzes the uptake of carbohydrates and their conversion into their respective phosphoesters during transport. There are four successive phosphoryl transfers in the PTS. Initial autophosphorylation of EI, using PEP as a substrate, is followed by transfer of the phosphoryl group from EI to HPr. EIIA catalyzes the self-phosphoryl transfer from HPr after which the phosphoryl group is transferred to histidine or cysteine residues of EIIB. The sugar is transported through the membrane-bound EIIC and is phosphorylated by the appropriate sugar-specific EIIB.
Class
Environmental Information Processing; Membrane transport
BRITE hierarchy
Pathway map
Phosphotransferase system (PTS)
ecf02060

All organismsOrtholog table
Module
PTS system, glucose-specific II component [PATH:ecf02060]
PTS system, maltose and glucose-specific II component [PATH:ecf02060]
PTS system, N-acetylglucosamine-specific II component [PATH:ecf02060]
PTS system, arbutin-like II component [PATH:ecf02060]
PTS system, trehalose-specific II component [PATH:ecf02060]
PTS system, arbutin-, cellobiose-, and salicin-specific II component [PATH:ecf02060]
PTS system, fructose-specific II component [PATH:ecf02060]
PTS system, mannitol-specific II component [PATH:ecf02060]
PTS system, cellobiose-specific II component [PATH:ecf02060]
PTS system, mannose-specific II component [PATH:ecf02060]
PTS system, N-acetylgalactosamine-specific II component [PATH:ecf02060]
PTS system, galactitol-specific II component [PATH:ecf02060]
PTS system, ascorbate-specific II component [PATH:ecf02060]
PTS system, N-acetylmuramic acid-specific II component [PATH:ecf02060]
Other DBs
BSID: 
GO: 
TC: 
Organism
Escherichia coli O157:H7 EC4115 (EHEC) [GN:ecf]
Gene
ptsI; phosphoenolpyruvate-protein phosphotransferase [KO:K08483] [EC:2.7.3.9]
fryA; multiphosphoryl transfer protein 1 [KO:K08483 K11189 K11201] [EC:2.7.3.9 2.7.1.69]
ptsA; multi-phosphoryl transfer protein 2 [KO:K08483 K11189 K11201] [EC:2.7.3.9 2.7.1.69]
ptsH; PTS system phosphohistidinoprotein-hexose phosphotransferase Hpr [KO:K02784]
crr; PTS system glucose-specific transporter [KO:K02777] [EC:2.7.1.69]
ptsG; PTS system glucose-specific transporter subunit IIBC [KO:K02778 K02779] [EC:2.7.1.69]
nagE; PTS system N-acetyl glucosamine specific transporter subunit IIABC [KO:K02802 K02803 K02804] [EC:2.7.1.69 2.7.1.69]
malX; PTS system maltose and glucose-specific transporter subunits IICB [KO:K02790 K02791] [EC:2.7.1.69]
ascF; PTS system cellobiose/arbutin/salicin-specific transporter subunit IIBC [KO:K02752 K02753] [EC:2.7.1.69]
treB; PTS system trehalose(maltose)-specific transporter subunit IIBC [KO:K02818 K02819] [EC:2.7.1.69]
murP; PTS system N-acetylmuramic acid transporter subunit IIBC [KO:K11191 K11192] [EC:2.7.1.69]
PTS system; K02749 PTS system, arbutin-like IIB component [EC:2.7.1.69] K02750 PTS system, arbutin-like IIC component [KO:K02749 K02750] [EC:2.7.1.69]
chbA; PTS system N,N'-diacetylchitobiose-specific transporter subunit IIA [KO:K02759] [EC:2.7.1.69]
chbB; PTS system N,N'-diacetylchitobiose-specific transporter subunit IIB [KO:K02760] [EC:2.7.1.69]
chbC; PTS system N,N'-diacetylchitobiose-specific transporter subunit IIC [KO:K02761]
mtlA; PTS system mannitol-specific transporter subunit IIABC [KO:K02798 K02799 K02800] [EC:2.7.1.69 2.7.1.69]
cmtB; mannitol phosphotransferase subunit EIIA [KO:K02798] [EC:2.7.1.69]
PTS system mannitol-specific transporter subunit cryptic EIICB; K02799 PTS system, mannitol-specific IIB component [EC:2.7.1.69] K02800 PTS system, mannitol-specific IIC component [KO:K02799 K02800] [EC:2.7.1.69]
manX; PTS system mannose-specific transporter subunit IIAB [KO:K02793 K02794] [EC:2.7.1.69 2.7.1.69]
manY; PTS system mannose-specific transporter subunit IIC [KO:K02795]
manZ; PTS system mannose-specific transporter subunit IID [KO:K02796]
PTS system mannose/fructose/sorbose family transporter subunit IIA; K02812 PTS system, sorbose-specific IIA component [EC:2.7.1.69] [KO:K02812] [EC:2.7.1.69]
PTS system mannose/fructose/sorbose family transporter subunit IIC; K02814 PTS system, sorbose-specific IIC component [KO:K02814]
PTS system mannose/fructose/sorbose family transporter subunit IID; K02815 PTS system, sorbose-specific IID component [KO:K02815]
agaF; PTS system mannose/sorbose-specific transporter subunit IIA [KO:K02744] [EC:2.7.1.69]
agaV; PTS system N-acetylgalactosamine-specific transporter subunit IIB [KO:K02745] [EC:2.7.1.69]
agaW; PTS system N-acetylgalactosamine-specific transporter subunit IIC [KO:K02746]
agaE; PTS system N-acetylgalactosamine-specific, IID component [KO:K02747]
agaB; PTS system N-acetylgalactosamine-specific transporter subunit IIB [KO:K10984] [EC:2.7.1.69]
agaD; PTS system N-acetylgalactosamine-specific transporter subunit IID [KO:K10986]
srlB; PTS system glucitol/sorbitol-specific transporter subunit IIA [KO:K02781] [EC:2.7.1.69]
PTS system sugar transporter subunit IIA; K02773 PTS system, galactitol-specific IIA component [EC:2.7.1.69] [KO:K02773] [EC:2.7.1.69]
gatA; PTS system galactitol-specific transporter subunit IIA [KO:K02773] [EC:2.7.1.69]
PTS system sugar transporter subunit IIB; K02774 PTS system, galactitol-specific IIB component [EC:2.7.1.69] [KO:K02774] [EC:2.7.1.69]
gatB; PTS system galactitol-specific transporter subunit IIB [KO:K02774] [EC:2.7.1.69]
PTS system sugar transporter subunit IIC; K02775 PTS system, galactitol-specific IIC component [KO:K02775]
gatC; PTS system galactitol-specific transporter subunit IIC [KO:K02775]
PTS system transporter subunit IIA domain-containing protein; K02821 PTS system, ascorbate-specific IIA component [EC:2.7.1.69] [KO:K02821] [EC:2.7.1.69]
ulaC; PTS system L-ascorbate-specific transporter subunit IIA [KO:K02821] [EC:2.7.1.69]
ulaB; PTS system L-ascorbate-specific transporter subunit IIB [KO:K02822] [EC:2.7.1.69]
ulaA; PTS system ascorbate-specific transporter subunit IIC [KO:K03475]
PTS system ascorbate-specific transporter subunit IIC; K03475 PTS system, ascorbate-specific IIC component [KO:K03475]
fruB; PTS system fructose-specific transporter subunit IIA/HPr protein [KO:K02768 K11183] [EC:2.7.1.69]
fruA; PTS system fructose-specific transporter subunit IIBC [KO:K02769 K02770] [EC:2.7.1.69]
ptsP; fused phosphoenolpyruvate-protein phosphotransferase PtsP/GAF domain [KO:K08484] [EC:2.7.3.9]
ptsO; PTS system phosphohistidinoprotein-hexose phosphotransferase Npr [KO:K08485]
ptsN; PTS system transporter subunit IIA-like nitrogen-regulatory protein PtsN [KO:K02806] [EC:2.7.1.69]
Compound
C00022  
Pyruvate
C00031  
D-Glucose
C00072  
Ascorbate
C00074  
Phosphoenolpyruvate
C00089  
Sucrose
C00092  
D-Glucose 6-phosphate
C00095  
D-Fructose
C00140  
N-Acetyl-D-glucosamine
C00159  
D-Mannose
C00185  
Cellobiose
C00208  
Maltose
C00243  
Lactose
C00275  
D-Mannose 6-phosphate
C00329  
D-Glucosamine
C00352  
D-Glucosamine 6-phosphate
C00357  
N-Acetyl-D-glucosamine 6-phosphate
C00392  
Mannitol
C00644  
D-Mannitol 1-phosphate
C00689  
alpha,alpha'-Trehalose 6-phosphate
C00697  
Nitrogen
C00794  
D-Sorbitol
C00963  
beta-D-Glucoside
C01083  
alpha,alpha-Trehalose
C01094  
D-Fructose 1-phosphate
C01096  
Sorbitol 6-phosphate
C01132  
N-Acetyl-D-galactosamine
C01135  
Phospho-beta-D-glucoside
C01451  
Salicin
C01452  
Sorbose
C01697  
Galactitol
C02262  
D-Galactosamine
C02713  
N-Acetylmuramate
C02888  
Sorbose 1-phosphate
C03752  
2-Amino-2-deoxy-D-gluconate
C05396  
Lactose 6-phosphate
C05737  
Maltose 6-phosphate
C06186  
Arbutin
C06187  
Arbutin 6-phosphate
C06188  
Salicin 6-phosphate
C06311  
Galactitol 1-phosphate
C06376  
N-Acetyl-D-galactosamine 6-phosphate
C06377  
D-Galactosamine 6-phosphate
C11544  
2(alpha-D-Mannosyl)-D-glycerate
C16186  
L-Ascorbate 6-phosphate
C16688  
Sucrose 6-phosphate
C16698  
N-Acetylmuramic acid 6-phosphate
C16699  
2-O-(6-Phospho-alpha-mannosyl)-D-glycerate
C20589  
6-Phospho-D-glucosaminate
Reference
  Authors
Darbon E, Galinier A, Le Coq D, Deutscher J.
  Title
Phosphotransfer functions mutated Bacillus subtilis HPr-like protein Crh carrying a histidine in the active site.
  Journal
J Mol Microbiol Biotechnol 3:439-44 (2001)
Reference
  Authors
Dahl U, Jaeger T, Nguyen BT, Sattler JM, Mayer C.
  Title
Identification of a phosphotransferase system of Escherichia coli required for growth on N-acetylmuramic acid.
  Journal
J Bacteriol 186:2385-92 (2004)
Reference
PMID:2117666
  Authors
Martin-Verstraete I, Debarbouille M, Klier A, Rapoport G
  Title
Levanase operon of Bacillus subtilis includes a fructose-specific phosphotransferase system regulating the expression of the operon.
  Journal
J Mol Biol 214:657-71 (1990)
Reference
  Authors
Kristich CJ, Glekas GD, Ordal GW.
  Title
The conserved cytoplasmic module of the transmembrane chemoreceptor McpC mediates carbohydrate chemotaxis in Bacillus subtilis.
  Journal
Mol Microbiol 47:1353-66 (2003)
Reference
  Authors
Sampaio MM, Chevance F, Dippel R, Eppler T, Schlegel A, Boos W, Lu YJ, Rock CO.
  Title
Phosphotransferase-mediated transport of the osmolyte 2-O-alpha-mannosyl-D-glycerate in Escherichia coli occurs by the product of the mngA (hrsA) gene and is regulated by the mngR (farR) gene product acting as repressor.
  Journal
J Biol Chem 279:5537-48 (2004)
Reference
  Authors
Keseler IM, Collado-Vides J, Gama-Castro S, Ingraham J, Paley S, Paulsen IT, Peralta-Gil M, Karp PD.
  Title
EcoCyc: a comprehensive database resource for Escherichia coli.
  Journal
Nucleic Acids Res 33:D334-7 (2005)
Reference
  Authors
Mukherjee A, Mammel MK, LeClerc JE, Cebula TA.
  Title
Altered utilization of N-acetyl-D-galactosamine by Escherichia coli O157:H7 from the 2006 spinach outbreak.
  Journal
J Bacteriol 190:1710-7 (2008)
Reference
  Authors
Brinkkotter A, Kloss H, Alpert C, Lengeler JW.
  Title
Pathways for the utilization of N-acetyl-galactosamine and galactosamine in Escherichia coli.
  Journal
Mol Microbiol 37:125-35 (2000)
Reference
  Authors
Tchieu JH, Norris V, Edwards JS, Saier MH Jr.
  Title
The complete phosphotranferase system in Escherichia coli.
  Journal
J Mol Microbiol Biotechnol 3:329-46 (2001)
Reference
  Authors
Kotrba P, Inui M, Yukawa H.
  Title
Bacterial phosphotransferase system (PTS) in carbohydrate uptake and control of carbon metabolism.
  Journal
J Biosci Bioeng 92:502-17 (2001)
KO pathway
 

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