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Entry
eco02060                    Pathway                                

Name
Phosphotransferase system (PTS) - Escherichia coli K-12 MG1655
Description
The phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS) is a major mechanism used by bacteria for uptake of carbohydrates, particularly hexoses, hexitols, and disaccharides, where the source of energy is from PEP. The PTS consists of two general components, enzyme I (EI) and histidine phosphocarrier protein (HPr), and of membrane-bound sugar specific permeases (enzymes II). Each enzyme II (EII) complex consists of one or two hydrophobic integral membrane domains (domains C and D) and two hydrophilic domains (domains A and B). EII complexes may exist as distinct proteins or as a single multidomain protein. The PTS catalyzes the uptake of carbohydrates and their conversion into their respective phosphoesters during transport. There are four successive phosphoryl transfers in the PTS. Initial autophosphorylation of EI, using PEP as a substrate, is followed by transfer of the phosphoryl group from EI to HPr. EIIA catalyzes the self-phosphoryl transfer from HPr after which the phosphoryl group is transferred to histidine or cysteine residues of EIIB. The sugar is transported through the membrane-bound EIIC and is phosphorylated by the appropriate sugar-specific EIIB.
Class
Environmental Information Processing; Membrane transport
BRITE hierarchy
Pathway map
Phosphotransferase system (PTS)
eco02060

All organismsOrtholog table
Module
PTS system, glucose-specific II component [PATH:eco02060]
PTS system, maltose and glucose-specific II component [PATH:eco02060]
PTS system, N-acetylglucosamine-specific II component [PATH:eco02060]
PTS system, trehalose-specific II component [PATH:eco02060]
PTS system, beta-glucosides-specific II component [PATH:eco02060]
PTS system, arbutin-, cellobiose-, and salicin-specific II component [PATH:eco02060]
PTS system, fructose-specific II component [PATH:eco02060]
PTS system, mannitol-specific II component [PATH:eco02060]
PTS system, cellobiose-specific II component [PATH:eco02060]
PTS system, mannose-specific II component [PATH:eco02060]
PTS system, galactitol-specific II component [PATH:eco02060]
PTS system, glucitol/sorbitol-specific II component [PATH:eco02060]
PTS system, ascorbate-specific II component [PATH:eco02060]
PTS system, N-acetylmuramic acid-specific II component [PATH:eco02060]
PTS system, 2-O-A-mannosyl-D-glycerate-specific II component [PATH:eco02060]
Other DBs
BSID: 
GO: 
TC: 
Organism
Escherichia coli K-12 MG1655 [GN:eco]
Gene
b2416  
ptsI; PEP-protein phosphotransferase of PTS system (enzyme I) [KO:K08483] [EC:2.7.3.9]
b3947  
ptsA; fused predicted PTS enzymes: Hpr component/enzyme I component/enzyme IIA component [KO:K08483 K11189 K11201] [EC:2.7.3.9 2.7.1.69]
b2383  
fryA; fused predicted PTS enzymes: Hpr component/enzyme I component/enzyme IIA component [KO:K08483 K11189 K11201] [EC:2.7.3.9 2.7.1.69]
b2415  
ptsH; phosphohistidinoprotein-hexose phosphotransferase component of PTS system (Hpr) [KO:K02784]
b2417  
crr; glucose-specific enzyme IIA component of PTS [KO:K02777] [EC:2.7.1.69]
b1101  
ptsG; fused glucose-specific PTS enzymes: IIB component/IIC component [KO:K02778 K02779] [EC:2.7.1.69]
b0679  
nagE; fused N-acetyl glucosamine specific PTS enzyme: IIC, IIB, and IIA components [KO:K02802 K02803 K02804] [EC:2.7.1.69 2.7.1.69]
b1621  
malX; fused maltose and glucose-specific PTS enzymes: IIB component, IIC component [KO:K02790 K02791] [EC:2.7.1.69]
b3722  
bglF; fused beta-glucoside-specific PTS enzymes: IIA component/IIB component/IIC component [KO:K02755 K02756 K02757] [EC:2.7.1.69 2.7.1.69]
b2715  
ascF; fused cellobiose/arbutin/salicin-specific PTS enzymes: IIB component/IC component [KO:K02752 K02753] [EC:2.7.1.69]
b4240  
treB; fused trehalose(maltose)-specific PTS enzyme: IIB component/IIC component [KO:K02818 K02819] [EC:2.7.1.69]
b2429  
murP; N-acetylmuramic acid permease, EIIBC component, PTS system [KO:K11191 K11192] [EC:2.7.1.69]
b1736  
chbA; N,N'-diacetylchitobiose-specific enzyme IIA component of PTS [KO:K02759] [EC:2.7.1.69]
b1738  
chbB; N,N'-diacetylchitobiose-specific enzyme IIB component of PTS [KO:K02760] [EC:2.7.1.69]
b1737  
chbC; N,N'-diacetylchitobiose-specific enzyme IIC component of PTS [KO:K02761]
b3599  
mtlA; fused mannitol-specific PTS enzymes: IIA components/IIB components/IIC components [KO:K02798 K02799 K02800] [EC:2.7.1.69 2.7.1.69]
b2934  
cmtB; predicted mannitol-specific enzyme IIA component of PTS [KO:K02798] [EC:2.7.1.69]
b2933  
cmtA; predicted fused mannitol-specific PTS enzymes: IIB component/IIC component [KO:K02799 K02800] [EC:2.7.1.69]
b0731  
mngA; fused 2-O-a-mannosyl-D-glycerate specific PTS enzymes: IIA component/IIB component/IIC component [KO:K11198 K11199 K11200] [EC:2.7.1.69 2.7.1.69]
b1817  
manX; fused mannose-specific PTS enzymes: IIA component/IIB component [KO:K02793 K02794] [EC:2.7.1.69 2.7.1.69]
b1818  
manY; mannose-specific enzyme IIC component of PTS [KO:K02795]
b1819  
manZ; mannose-specific enzyme IID component of PTS [KO:K02796]
b3133  
agaV; N-acetylgalactosamine-specific enzyme IIB component of PTS [KO:K02745] [EC:2.7.1.69]
b3138  
agaB; N-acetylgalactosamine-specific enzyme IIB component of PTS [KO:K10984] [EC:2.7.1.69]
b3139  
agaC; N-acetylgalactosamine-specific enzyme IIC component of PTS [KO:K10985]
b3140  
agaD; N-acetylgalactosamine-specific enzyme IID component of PTS [KO:K10986]
b2704  
srlB; glucitol/sorbitol-specific enzyme IIA component of PTS [KO:K02781] [EC:2.7.1.69]
b2703  
srlE; glucitol/sorbitol-specific enzyme IIB component of PTS [KO:K02782 K02783] [EC:2.7.1.69]
b2702  
srlA; glucitol/sorbitol-specific enzyme IIC component of PTS [KO:K02783]
b4302  
sgcA; KpLE2 phage-like element [KO:K02773] [EC:2.7.1.69]
b2094  
gatA; galactitol-specific enzyme IIA component of PTS [KO:K02773] [EC:2.7.1.69]
b2093  
gatB; galactitol-specific enzyme IIB component of PTS [KO:K02774] [EC:2.7.1.69]
b4565  
sgcB; predicted enzyme IIB component of PTS [KO:K02774] [EC:2.7.1.69]
b4304  
sgcC; KpLE2 phage-like element [KO:K02775]
b0129  
yadI; predicted PTS Enzyme IIA [KO:K02821] [EC:2.7.1.69]
b4195  
ulaC; L-ascorbate-specific enzyme IIA component of PTS [KO:K02821] [EC:2.7.1.69]
b4194  
ulaB; L-ascorbate-specific enzyme IIB component of PTS [KO:K02822] [EC:2.7.1.69]
b4193  
ulaA; L-ascorbate-specific enzyme IIC component of PTS [KO:K03475]
b2169  
fruB; fused fructose-specific PTS enzymes: IIA component/HPr component [KO:K02768 K11183] [EC:2.7.1.69]
b2167  
fruA; fused fructose-specific PTS enzymes: IIBcomponent/IIC components [KO:K02769 K02770] [EC:2.7.1.69]
b2829  
ptsP; fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein [KO:K08484] [EC:2.7.3.9]
b3206  
npr; phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) [KO:K08485]
b3204  
ptsN; sugar-specific enzyme IIA component of PTS [KO:K02806] [EC:2.7.1.69]
Compound
C00022  
Pyruvate
C00031  
D-Glucose
C00072  
Ascorbate
C00074  
Phosphoenolpyruvate
C00089  
Sucrose
C00092  
D-Glucose 6-phosphate
C00095  
D-Fructose
C00140  
N-Acetyl-D-glucosamine
C00159  
D-Mannose
C00185  
Cellobiose
C00208  
Maltose
C00243  
Lactose
C00275  
D-Mannose 6-phosphate
C00329  
D-Glucosamine
C00352  
D-Glucosamine 6-phosphate
C00357  
N-Acetyl-D-glucosamine 6-phosphate
C00392  
Mannitol
C00644  
D-Mannitol 1-phosphate
C00689  
alpha,alpha'-Trehalose 6-phosphate
C00697  
Nitrogen
C00794  
D-Sorbitol
C00963  
beta-D-Glucoside
C01083  
alpha,alpha-Trehalose
C01094  
D-Fructose 1-phosphate
C01096  
Sorbitol 6-phosphate
C01132  
N-Acetyl-D-galactosamine
C01135  
Phospho-beta-D-glucoside
C01451  
Salicin
C01452  
Sorbose
C01697  
Galactitol
C02262  
D-Galactosamine
C02713  
N-Acetylmuramate
C02888  
Sorbose 1-phosphate
C03752  
2-Amino-2-deoxy-D-gluconate
C05396  
Lactose 6-phosphate
C05737  
Maltose 6-phosphate
C06186  
Arbutin
C06187  
Arbutin 6-phosphate
C06188  
Salicin 6-phosphate
C06311  
Galactitol 1-phosphate
C06376  
N-Acetyl-D-galactosamine 6-phosphate
C06377  
D-Galactosamine 6-phosphate
C11544  
2(alpha-D-Mannosyl)-D-glycerate
C16186  
L-Ascorbate 6-phosphate
C16688  
Sucrose 6-phosphate
C16698  
N-Acetylmuramic acid 6-phosphate
C16699  
2-O-(6-Phospho-alpha-mannosyl)-D-glycerate
C20589  
D-Glucosaminate-6-phosphate
Reference
  Authors
Darbon E, Galinier A, Le Coq D, Deutscher J.
  Title
Phosphotransfer functions mutated Bacillus subtilis HPr-like protein Crh carrying a histidine in the active site.
  Journal
J Mol Microbiol Biotechnol 3:439-44 (2001)
Reference
  Authors
Dahl U, Jaeger T, Nguyen BT, Sattler JM, Mayer C.
  Title
Identification of a phosphotransferase system of Escherichia coli required for growth on N-acetylmuramic acid.
  Journal
J Bacteriol 186:2385-92 (2004)
Reference
PMID:2117666
  Authors
Martin-Verstraete I, Debarbouille M, Klier A, Rapoport G
  Title
Levanase operon of Bacillus subtilis includes a fructose-specific phosphotransferase system regulating the expression of the operon.
  Journal
J Mol Biol 214:657-71 (1990)
Reference
  Authors
Kristich CJ, Glekas GD, Ordal GW.
  Title
The conserved cytoplasmic module of the transmembrane chemoreceptor McpC mediates carbohydrate chemotaxis in Bacillus subtilis.
  Journal
Mol Microbiol 47:1353-66 (2003)
Reference
  Authors
Sampaio MM, Chevance F, Dippel R, Eppler T, Schlegel A, Boos W, Lu YJ, Rock CO.
  Title
Phosphotransferase-mediated transport of the osmolyte 2-O-alpha-mannosyl-D-glycerate in Escherichia coli occurs by the product of the mngA (hrsA) gene and is regulated by the mngR (farR) gene product acting as repressor.
  Journal
J Biol Chem 279:5537-48 (2004)
Reference
  Authors
Keseler IM, Collado-Vides J, Gama-Castro S, Ingraham J, Paley S, Paulsen IT, Peralta-Gil M, Karp PD.
  Title
EcoCyc: a comprehensive database resource for Escherichia coli.
  Journal
Nucleic Acids Res 33:D334-7 (2005)
Reference
  Authors
Mukherjee A, Mammel MK, LeClerc JE, Cebula TA.
  Title
Altered utilization of N-acetyl-D-galactosamine by Escherichia coli O157:H7 from the 2006 spinach outbreak.
  Journal
J Bacteriol 190:1710-7 (2008)
Reference
  Authors
Brinkkotter A, Kloss H, Alpert C, Lengeler JW.
  Title
Pathways for the utilization of N-acetyl-galactosamine and galactosamine in Escherichia coli.
  Journal
Mol Microbiol 37:125-35 (2000)
Reference
  Authors
Tchieu JH, Norris V, Edwards JS, Saier MH Jr.
  Title
The complete phosphotranferase system in Escherichia coli.
  Journal
J Mol Microbiol Biotechnol 3:329-46 (2001)
Reference
  Authors
Kotrba P, Inui M, Yukawa H.
  Title
Bacterial phosphotransferase system (PTS) in carbohydrate uptake and control of carbon metabolism.
  Journal
J Biosci Bioeng 92:502-17 (2001)
KO pathway
 

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