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Entry
eco02060                    Pathway                                

Name
Phosphotransferase system (PTS) - Escherichia coli K-12 MG1655
Description
The phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS) is a major mechanism used by bacteria for uptake of carbohydrates, particularly hexoses, hexitols, and disaccharides, where the source of energy is from PEP. The PTS consists of two general components, enzyme I (EI) and histidine phosphocarrier protein (HPr), and of membrane-bound sugar specific permeases (enzymes II). Each enzyme II (EII) complex consists of one or two hydrophobic integral membrane domains (domains C and D) and two hydrophilic domains (domains A and B). EII complexes may exist as distinct proteins or as a single multidomain protein. The PTS catalyzes the uptake of carbohydrates and their conversion into their respective phosphoesters during transport. There are four successive phosphoryl transfers in the PTS. Initial autophosphorylation of EI, using PEP as a substrate, is followed by transfer of the phosphoryl group from EI to HPr. EIIA catalyzes the self-phosphoryl transfer from HPr after which the phosphoryl group is transferred to histidine or cysteine residues of EIIB. The sugar is transported through the membrane-bound EIIC and is phosphorylated by the appropriate sugar-specific EIIB.
Class
Environmental Information Processing; Membrane transport
BRITE hierarchy
Pathway map
eco02060  Phosphotransferase system (PTS)
eco02060

Ortholog table
Module
eco_M00265  PTS system, glucose-specific II component [PATH:eco02060]
eco_M00266  PTS system, maltose/glucose-specific II component [PATH:eco02060]
eco_M00267  PTS system, N-acetylglucosamine-specific II component [PATH:eco02060]
eco_M00270  PTS system, trehalose-specific II component [PATH:eco02060]
eco_M00271  PTS system, beta-glucoside-specific II component [PATH:eco02060]
eco_M00272  PTS system, beta-glucoside (arbutin/salicin/cellobiose)-specific II component [PATH:eco02060]
eco_M00273  PTS system, fructose-specific II component [PATH:eco02060]
eco_M00274  PTS system, mannitol-specific II component [PATH:eco02060]
eco_M00275  PTS system, cellobiose-specific II component [PATH:eco02060]
eco_M00276  PTS system, mannose-specific II component [PATH:eco02060]
eco_M00279  PTS system, galactitol-specific II component [PATH:eco02060]
eco_M00280  PTS system, glucitol/sorbitol-specific II component [PATH:eco02060]
eco_M00283  PTS system, ascorbate-specific II component [PATH:eco02060]
eco_M00303  PTS system, N-acetylmuramic acid-specific II component [PATH:eco02060]
eco_M00305  PTS system, 2-O-A-mannosyl-D-glycerate-specific II component [PATH:eco02060]
Other DBs
BSID: 1119
GO: 0009401
TC: 4.A
Organism
Escherichia coli K-12 MG1655 [GN:eco]
Gene
b2416  ptsI; PEP-protein phosphotransferase of PTS system (enzyme I) [KO:K08483] [EC:2.7.3.9]
b3947  frwA; putative PTS enzyme: Hpr, enzyme I and II components [KO:K08483 K11189 K11201] [EC:2.7.3.9 2.7.1.-]
b2383  fryA; putative PTS enzyme: Hpr, enzyme I and IIA components [KO:K08483 K11189 K11201] [EC:2.7.3.9 2.7.1.-]
b2415  ptsH; phosphohistidinoprotein-hexose phosphotransferase component of PTS system (Hpr) [KO:K02784]
b2417  crr; glucose-specific enzyme IIA component of PTS [KO:K02777] [EC:2.7.1.-]
b1101  ptsG; fused glucose-specific PTS enzymes: IIB component/IIC component [KO:K02778 K02779] [EC:2.7.1.199]
b0679  nagE; N-acetyl glucosamine specific PTS enzyme IIC, IIB, and IIA components [KO:K02802 K02803 K02804] [EC:2.7.1.193 2.7.1.193]
b1621  malX; maltose and glucose-specific PTS enzyme IIB component and IIC component [KO:K02790 K02791] [EC:2.7.1.208 2.7.1.199]
b3722  bglF; fused beta-glucoside-specific PTS enzymes: IIA component/IIB component/IIC component [KO:K02755 K02756 K02757] [EC:2.7.1.- 2.7.1.-]
b2715  ascF; cellobiose/arbutin/salicin-specific PTS enzymes, IIB and IC components [KO:K02752 K02753] [EC:2.7.1.-]
b4240  treB; trehalose-specific PTS enzyme: IIB and IIC component [KO:K02818 K02819] [EC:2.7.1.201]
b2429  murP; N-acetylmuramic acid permease, EIIBC component, PTS system [KO:K11191 K11192] [EC:2.7.1.192]
b3599  mtlA; mannitol-specific PTS enzyme: IIA, IIB and IIC components [KO:K02798 K02799 K02800] [EC:2.7.1.197 2.7.1.197]
b2934  cmtB; putative mannitol-specific enzyme IIA component of PTS [KO:K02798] [EC:2.7.1.197]
b2933  cmtA; putative mannitol-specific PTS IIB and IIC components [KO:K02799 K02800] [EC:2.7.1.197]
b0731  mngA; fused 2-O-a-mannosyl-D-glycerate specific PTS enzymes: IIA component/IIB component/IIC component [KO:K11198 K11199 K11200] [EC:2.7.1.195 2.7.1.195]
b1736  chbA; N,N'-diacetylchitobiose-specific enzyme IIA component of PTS [KO:K02759] [EC:2.7.1.205 2.7.1.196]
b1738  chbB; N,N'-diacetylchitobiose-specific enzyme IIB component of PTS [KO:K02760] [EC:2.7.1.205 2.7.1.196]
b1737  chbC; N,N'-diacetylchitobiose-specific enzyme IIC component of PTS [KO:K02761]
b1817  manX; fused mannose-specific PTS enzymes: IIA component/IIB component [KO:K02793 K02794] [EC:2.7.1.191 2.7.1.191]
b1818  manY; mannose-specific enzyme IIC component of PTS [KO:K02795]
b1819  manZ; mannose-specific enzyme IID component of PTS [KO:K02796]
b3133  agaV; N-acetylgalactosamine-specific enzyme IIB component of PTS [KO:K02745] [EC:2.7.1.-]
b3138  agaB; N-acetylgalactosamine-specific enzyme IIB component of PTS [KO:K10984] [EC:2.7.1.-]
b3139  agaC; N-acetylgalactosamine-specific enzyme IIC component of PTS [KO:K10985]
b3140  agaD; N-acetylgalactosamine-specific enzyme IID component of PTS [KO:K10986]
b2704  srlB; glucitol/sorbitol-specific enzyme IIA component of PTS [KO:K02781] [EC:2.7.1.198]
b2703  srlE; glucitol/sorbitol-specific enzyme IIB component of PTS [KO:K02782 K02783] [EC:2.7.1.198]
b2702  srlA; glucitol/sorbitol-specific enzyme IIC component of PTS [KO:K02783]
b4302  sgcA; putative phosphotransferase enzyme IIA component [KO:K02773] [EC:2.7.1.200]
b2094  gatA; galactitol-specific enzyme IIA component of PTS [KO:K02773] [EC:2.7.1.200]
b2093  gatB; galactitol-specific enzyme IIB component of PTS [KO:K02774] [EC:2.7.1.200]
b4565  sgcB; putative enzyme IIB component of PTS [KO:K02774] [EC:2.7.1.200]
b4304  sgcC; putative PTS system EIIC permease component [KO:K02775]
b4195  ulaC; L-ascorbate-specific enzyme IIA component of PTS [KO:K02821] [EC:2.7.1.194]
b4194  ulaB; L-ascorbate-specific enzyme IIB component of PTS [KO:K02822] [EC:2.7.1.194]
b4193  ulaA; L-ascorbate-specific enzyme IIC permease component of PTS [KO:K03475]
b2169  fruB; fused fructose-specific PTS enzymes: IIA component/HPr component [KO:K02768 K11183] [EC:2.7.1.202]
b2167  fruA; fused fructose-specific PTS enzymes: IIBcomponent/IIC components [KO:K02769 K02770] [EC:2.7.1.202]
b2829  ptsP; PEP-protein phosphotransferase enzyme I; GAF domain containing protein [KO:K08484] [EC:2.7.3.9]
b3206  npr; phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) [KO:K08485]
b3204  ptsN; sugar-specific enzyme IIA component of PTS [KO:K02806] [EC:2.7.1.-]
Compound
C00022  Pyruvate
C00031  D-Glucose
C00072  Ascorbate
C00074  Phosphoenolpyruvate
C00089  Sucrose
C00092  D-Glucose 6-phosphate
C00095  D-Fructose
C00124  D-Galactose
C00140  N-Acetyl-D-glucosamine
C00159  D-Mannose
C00185  Cellobiose
C00208  Maltose
C00243  Lactose
C00275  D-Mannose 6-phosphate
C00329  D-Glucosamine
C00352  D-Glucosamine 6-phosphate
C00357  N-Acetyl-D-glucosamine 6-phosphate
C00392  Mannitol
C00644  D-Mannitol 1-phosphate
C00689  alpha,alpha'-Trehalose 6-phosphate
C00697  Nitrogen
C00794  D-Sorbitol
C01083  alpha,alpha-Trehalose
C01094  D-Fructose 1-phosphate
C01096  Sorbitol 6-phosphate
C01113  D-Galactose 6-phosphate
C01132  N-Acetyl-D-galactosamine
C01451  Salicin
C01452  Sorbose
C01674  Chitobiose
C01697  Galactitol
C01798  D-Glucoside
C02262  D-Galactosamine
C02713  N-Acetylmuramate
C02888  Sorbose 1-phosphate
C02995  Maltose 6'-phosphate
C03752  2-Amino-2-deoxy-D-gluconate
C04534  6-Phospho-beta-D-glucosyl-(1,4)-D-glucose
C05396  Lactose 6'-phosphate
C06186  Arbutin
C06187  Arbutin 6-phosphate
C06188  Salicin 6-phosphate
C06311  Galactitol 1-phosphate
C06376  N-Acetyl-D-galactosamine 6-phosphate
C06377  D-Galactosamine 6-phosphate
C11544  2-O-(alpha-D-Mannosyl)-D-glycerate
C16186  L-Ascorbate 6-phosphate
C16488  Fructoselysine
C16489  Fructoselysine 6-phosphate
C16688  Sucrose 6-phosphate
C16698  N-Acetylmuramic acid 6-phosphate
C16699  2-O-(6-Phospho-alpha-mannosyl)-D-glycerate
C20589  D-Glucosaminate-6-phosphate
C20978  Glucoselysine
C20979  Glucoselysine-6-phosphate
C21152  N,N'-Diacetylchitobiose 6'-phosphate
Reference
  Authors
Darbon E, Galinier A, Le Coq D, Deutscher J.
  Title
Phosphotransfer functions mutated Bacillus subtilis HPr-like protein Crh carrying a histidine in the active site.
  Journal
J Mol Microbiol Biotechnol 3:439-44 (2001)
Reference
  Authors
Dahl U, Jaeger T, Nguyen BT, Sattler JM, Mayer C.
  Title
Identification of a phosphotransferase system of Escherichia coli required for growth on N-acetylmuramic acid.
  Journal
J Bacteriol 186:2385-92 (2004)
DOI:10.1128/JB.186.8.2385-2392.2004
Reference
PMID:2117666
  Authors
Martin-Verstraete I, Debarbouille M, Klier A, Rapoport G
  Title
Levanase operon of Bacillus subtilis includes a fructose-specific phosphotransferase system regulating the expression of the operon.
  Journal
J Mol Biol 214:657-71 (1990)
DOI:10.1016/0022-2836(90)90284-S
Reference
  Authors
Kristich CJ, Glekas GD, Ordal GW.
  Title
The conserved cytoplasmic module of the transmembrane chemoreceptor McpC mediates carbohydrate chemotaxis in Bacillus subtilis.
  Journal
Mol Microbiol 47:1353-66 (2003)
DOI:10.1046/j.1365-2958.2003.03375.x
Reference
  Authors
Sampaio MM, Chevance F, Dippel R, Eppler T, Schlegel A, Boos W, Lu YJ, Rock CO.
  Title
Phosphotransferase-mediated transport of the osmolyte 2-O-alpha-mannosyl-D-glycerate in Escherichia coli occurs by the product of the mngA (hrsA) gene and is regulated by the mngR (farR) gene product acting as repressor.
  Journal
J Biol Chem 279:5537-48 (2004)
DOI:10.1074/jbc.M310980200
Reference
  Authors
Keseler IM, Collado-Vides J, Gama-Castro S, Ingraham J, Paley S, Paulsen IT, Peralta-Gil M, Karp PD.
  Title
EcoCyc: a comprehensive database resource for Escherichia coli.
  Journal
Nucleic Acids Res 33:D334-7 (2005)
DOI:10.1093/nar/gki108
Reference
  Authors
Mukherjee A, Mammel MK, LeClerc JE, Cebula TA.
  Title
Altered utilization of N-acetyl-D-galactosamine by Escherichia coli O157:H7 from the 2006 spinach outbreak.
  Journal
J Bacteriol 190:1710-7 (2008)
DOI:10.1128/JB.01737-07
Reference
  Authors
Brinkkotter A, Kloss H, Alpert C, Lengeler JW.
  Title
Pathways for the utilization of N-acetyl-galactosamine and galactosamine in Escherichia coli.
  Journal
Mol Microbiol 37:125-35 (2000)
DOI:10.1046/j.1365-2958.2000.01969.x
Reference
  Authors
Tchieu JH, Norris V, Edwards JS, Saier MH Jr.
  Title
The complete phosphotranferase system in Escherichia coli.
  Journal
J Mol Microbiol Biotechnol 3:329-46 (2001)
Reference
  Authors
Kotrba P, Inui M, Yukawa H.
  Title
Bacterial phosphotransferase system (PTS) in carbohydrate uptake and control of carbon metabolism.
  Journal
J Biosci Bioeng 92:502-17 (2001)
DOI:10.1016/S1389-1723(01)80308-X
KO pathway
ko02060   

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