KEGG   PATHWAY: hsa03030Help
Entry
hsa03030                    Pathway                                

Name
DNA replication - Homo sapiens (human)
Description
A complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (DnaB or MCM complex) precedes the DNA synthetic machinery and unwinds the duplex parental DNA in cooperation with the SSB or RPA. On the leading strand, replication occurs continuously in a 5 to 3 direction, whereas on the lagging strand, DNA replication occurs discontinuously by synthesis and joining of short Okazaki fragments. In prokaryotes, the leading strand replication apparatus consists of a DNA polymerase (pol III core), a sliding clamp (beta), and a clamp loader (gamma delta complex). The DNA primase (DnaG) is needed to form RNA primers. Normally, during replication of the lagging-strand DNA template, an RNA primer is removed either by an RNase H or by the 5 to 3 exonuclease activity of DNA pol I, and the DNA ligase joins the Okazaki fragments. In eukaryotes, three DNA polymerases (alpha, delta, and epsilon) have been identified. DNA primase forms a permanent complex with DNA polymerase alpha. PCNA and RFC function as a clamp and a clamp loader. FEN 1 and RNase H1 remove the RNA from the Okazaki fragments and DNA ligase I joins the DNA.
Class
Genetic Information Processing; Replication and repair
BRITE hierarchy
Pathway map
DNA replication
hsa03030

All organismsOrtholog table
Module
DNA polymerase alpha / primase complex [PATH:hsa03030]
DNA polymerase delta complex [PATH:hsa03030]
DNA polymerase epsilon complex [PATH:hsa03030]
MCM complex [PATH:hsa03030]
RPA complex [PATH:hsa03030]
RF-C complex [PATH:hsa03030]
Organism
Homo sapiens (human) [GN:hsa]
Gene
6742  
SSBP1; single stranded DNA binding protein 1 [KO:K03111]
246243  
RNASEH1; ribonuclease H1 [KO:K03469] [EC:3.1.26.4]
6117  
RPA1; replication protein A1 [KO:K07466]
5111  
PCNA; proliferating cell nuclear antigen [KO:K04802]
1763  
DNA2; DNA replication helicase/nuclease 2 [KO:K10742] [EC:3.6.4.12]
2237  
FEN1; flap structure-specific endonuclease 1 [KO:K04799] [EC:3.-.-.-]
3978  
LIG1; DNA ligase 1 [KO:K10747] [EC:6.5.1.7 6.5.1.6 6.5.1.1]
5422  
POLA1; DNA polymerase alpha 1, catalytic subunit [KO:K02320] [EC:2.7.7.7]
23649  
POLA2; DNA polymerase alpha 2, accessory subunit [KO:K02321]
5557  
PRIM1; primase (DNA) subunit 1 [KO:K02684] [EC:2.7.7.-]
5558  
PRIM2; primase (DNA) subunit 2 [KO:K02685] [EC:2.7.7.-]
5424  
POLD1; DNA polymerase delta 1, catalytic subunit [KO:K02327] [EC:2.7.7.7]
5425  
POLD2; DNA polymerase delta 2, accessory subunit [KO:K02328]
10714  
POLD3; DNA polymerase delta 3, accessory subunit [KO:K03504]
57804  
POLD4; DNA polymerase delta 4, accessory subunit [KO:K03505]
5426  
POLE; DNA polymerase epsilon, catalytic subunit [KO:K02324] [EC:2.7.7.7]
5427  
POLE2; DNA polymerase epsilon 2, accessory subunit [KO:K02325] [EC:2.7.7.7]
54107  
POLE3; DNA polymerase epsilon 3, accessory subunit [KO:K02326] [EC:2.7.7.7]
56655  
POLE4; DNA polymerase epsilon 4, accessory subunit [KO:K03506] [EC:2.7.7.7]
4171  
MCM2; minichromosome maintenance complex component 2 [KO:K02540] [EC:3.6.4.12]
4172  
MCM3; minichromosome maintenance complex component 3 [KO:K02541] [EC:3.6.4.12]
4173  
MCM4; minichromosome maintenance complex component 4 [KO:K02212] [EC:3.6.4.12]
4174  
MCM5; minichromosome maintenance complex component 5 [KO:K02209] [EC:3.6.4.12]
4175  
MCM6; minichromosome maintenance complex component 6 [KO:K02542] [EC:3.6.4.12]
4176  
MCM7; minichromosome maintenance complex component 7 [KO:K02210] [EC:3.6.4.12]
6118  
RPA2; replication protein A2 [KO:K10739]
29935  
RPA4; replication protein A4 [KO:K10741]
6119  
RPA3; replication protein A3 [KO:K10740]
5981  
RFC1; replication factor C subunit 1 [KO:K10754]
5984  
RFC4; replication factor C subunit 4 [KO:K10755]
5982  
RFC2; replication factor C subunit 2 [KO:K10755]
5985  
RFC5; replication factor C subunit 5 [KO:K10756]
5983  
RFC3; replication factor C subunit 3 [KO:K10756]
10535  
RNASEH2A; ribonuclease H2 subunit A [KO:K10743] [EC:3.1.26.4]
79621  
RNASEH2B; ribonuclease H2 subunit B [KO:K10744]
84153  
RNASEH2C; ribonuclease H2 subunit C [KO:K10745]
Reference
(Japanese)
  Authors
Matsukage A, Masai H.
  Title
[The Replication and Partitioning of Genomes] (In Japanese)
  Journal
Springer-Verlag Tokyo (2002)
Reference
(Japanese)
  Authors
Tamura T, Yamamoto M.
  Title
[Molecular Biology  Illustrated] (In Japanese)
  Journal
Yodosha (2002)
Reference
PMID:8087839
  Authors
Stillman B.
  Title
Smart machines at the DNA replication fork.
  Journal
Cell 78:725-8 (1994)
Reference
PMID:9759502
  Authors
Waga S, Stillman B.
  Title
The DNA replication fork in eukaryotic cells.
  Journal
Annu Rev Biochem 67:721-51 (1998)
KO pathway
 

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