KEGG   PATHWAY: ko00510Help
Entry
ko00510                     Pathway                                
Name
N-Glycan biosynthesis
Description
N-glycans or asparagine-linked glycans are major constituents of glycoproteins in eukaryotes. N-glycans are covalently attached to asparagine with the consensus sequence of Asn-X-Ser/Thr by an N-glycosidic bond, GlcNAc b1- Asn. Biosynthesis of N-glycans begins on the cytoplasmic face of the ER membrane with the transferase reaction of UDP-GlcNAc and the lipid-like precursor P-Dol (dolichol phosphate) to generate GlcNAc a1- PP-Dol. After sequential addition of monosaccharides by ALG glycosyltransferases [MD:M00055], the N-glycan precursor is attached by the OST (oligosaccharyltransferase) complex to the polypeptide chain that is being synthesized and translocated through the ER membrane. The protein-bound N-glycan precursor is subsequently trimmed, extended, and modified in the ER and Golgi by a complex series of reactions catalyzed by membrane-bound glycosidases and glycosyltransferases. N-glycans thus synthesized are classified into three types: high-mannose type, complex type, and hybrid type. Defects in N-glycan biosynthesis lead to a variety of human diseases known as congenital disorders of glycosylation [DS:H00118 H00119].
Class
Metabolism; Glycan biosynthesis and metabolism
BRITE hierarchy
Pathway map
N-Glycan biosynthesis
ko00510

All organismsOrtholog table
Module
M00055  
N-glycan precursor biosynthesis [PATH:ko00510]
M00072  
Oligosaccharyltransferase [PATH:ko00510]
M00073  
N-glycan precursor trimming [PATH:ko00510]
M00074  
N-glycan biosynthesis, high-mannose type [PATH:ko00510]
M00075  
N-glycan biosynthesis, complex type [PATH:ko00510]
Disease
H00118  
Congenital disorders of glycosylation (CDG) type I
H00119  
Congenital disorders of glycosylation (CDG) type II
H00768  
Nonsyndromic autosomal recessive mental retardation (NS-ARMR)
Other DBs
BSID: 
GO: 
Orthology
K00902  
dolichol kinase [EC:2.7.1.108]
K01001  
UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase [EC:2.7.8.15]
K00729  
dolichyl-phosphate beta-glucosyltransferase [EC:2.4.1.117]
K07432  
beta-1,4-N-acetylglucosaminyltransferase [EC:2.4.1.141]
K07441  
beta-1,4-N-acetylglucosaminyltransferase [EC:2.4.1.141]
K00721  
dolichol-phosphate mannosyltransferase [EC:2.4.1.83]
K09658  
dolichyl-phosphate mannosyltransferase polypeptide 2, regulatory subunit
K09659  
dolichyl-phosphate mannosyltransferase polypeptide 3
K03842  
beta-1,4-mannosyltransferase [EC:2.4.1.142]
K03843  
alpha-1,3/alpha-1,6-mannosyltransferase [EC:2.4.1.257 2.4.1.132]
K03844  
alpha-1,2-mannosyltransferase [EC:2.4.1.131]
K06316  
oligosaccharidyl-lipid flippase family
K03845  
alpha-1,3-mannosyltransferase [EC:2.4.1.258]
K03846  
alpha-1,2-mannosyltransferase [EC:2.4.1.261 2.4.1.259]
K03847  
alpha-1,6-mannosyltransferase [EC:2.4.1.260]
K03848  
alpha-1,3-glucosyltransferase [EC:2.4.1.267]
K03849  
alpha-1,3-glucosyltransferase [EC:2.4.1.265]
K03850  
alpha-1,2-glucosyltransferase [EC:2.4.1.256]
K07151  
dolichyl-diphosphooligosaccharide--protein glycosyltransferase [EC:2.4.99.18]
K12666  
oligosaccharyltransferase complex subunit alpha (ribophorin I)
K12667  
oligosaccharyltransferase complex subunit delta (ribophorin II)
K12668  
oligosaccharyltransferase complex subunit epsilon
K12669  
oligosaccharyltransferase complex subunit gamma
K12670  
oligosaccharyltransferase complex subunit beta
K00730  
oligosaccharyl transferase complex subunit OST4
K12691  
oligosaccharyl transferase complex subunit OST5
K07252  
dolichyldiphosphatase [EC:3.6.1.43]
K01228  
mannosyl-oligosaccharide glucosidase [EC:3.2.1.106]
K05546  
alpha 1,3-glucosidase [EC:3.2.1.84]
K01230  
mannosyl-oligosaccharide alpha-1,2-mannosidase [EC:3.2.1.113]
K00726  
alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase [EC:2.4.1.101]
K01231  
alpha-mannosidase II [EC:3.2.1.114]
K00736  
alpha-1,6-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase [EC:2.4.1.143]
K00717  
glycoprotein 6-alpha-L-fucosyltransferase [EC:2.4.1.68]
K07966  
beta-1,4-galactosyltransferase 1 [EC:2.4.1.- 2.4.1.38 2.4.1.90 2.4.1.22]
K07967  
beta-1,4-galactosyltransferase 2 [EC:2.4.1.- 2.4.1.38 2.4.1.90 2.4.1.22]
K07968  
beta-1,4-galactosyltransferase 3 [EC:2.4.1.- 2.4.1.38 2.4.1.90]
K00778  
beta-galactoside alpha-2,6-sialyltransferase (sialyltransferase 1) [EC:2.4.99.1]
K00779  
beta-galactoside alpha2,6-sialyltransferase (sialyltransferase 2) [EC:2.4.99.1]
K00737  
beta-1,4-mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase [EC:2.4.1.144]
K00738  
alpha-1,3-mannosylglycoprotein beta-1,4-N-acetylglucosaminyltransferase A/B [EC:2.4.1.145]
K00744  
alpha-1,3(6)-mannosylglycoprotein beta-1,6-N-acetyl-glucosaminyltransferase [EC:2.4.1.155]
K09661  
mannosyl alpha-1,6-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase, isozyme B [EC:2.4.1.-]
K13748  
alpha-1,3-mannosylglycoprotein beta-1,4-N-acetylglucosaminyltransferase C [EC:2.4.1.145 2.4.1.201]
Compound
C00096  
GDP-mannose
C00110  
Dolichyl phosphate
C00381  
Dolichol
C00621  
Dolichyl diphosphate
C01246  
Dolichyl beta-D-glucosyl phosphate
C03021  
Protein asparagine
C03862  
Dolichyl phosphate D-mannose
G00001  
N-Acetyl-D-glucosaminyldiphosphodolichol
G00002  
N,N'-Chitobiosyldiphosphodolichol
G00003  
 
G00004  
 
G00005  
 
G00006  
 
G00007  
 
G00008  
 
G00009  
 
G00010  
 
G00011  
 
G00012  
 
G00013  
 
G00014  
 
G00015  
 
G00016  
 
G00017  
 
G00018  
DS 3
G00019  
 
G00020  
 
G00021  
 
G00022  
 
G00171  
 
G10526  
 
G10595  
 
G10596  
 
G10597  
 
G10598  
 
G10599  
 
Reference
PMID:9111134
  Authors
Schutzbach JS.
  Title
The role of the lipid matrix in the biosynthesis of dolichyl-linked oligosaccharides.
  Journal
Glycoconj J 14:175-82 (1997)
Reference
  Authors
Oriol R, Martinez-Duncker I, Chantret I, Mollicone R, Codogno P.
  Title
Common origin and evolution of glycosyltransferases using Dol-P-monosaccharides as donor substrate.
  Journal
Mol Biol Evol 19:1451-63 (2002)
Reference
  Authors
Thiel C, Schwarz M, Peng J, Grzmil M, Hasilik M, Braulke T, Kohlschutter A, von Figura K, Lehle L, Korner C.
  Title
A new type of congenital disorders of glycosylation (CDG-Ii) provides new insights into the early steps of dolichol-linked oligosaccharide biosynthesis.
  Journal
J Biol Chem 278:22498-505 (2003)
Reference
  Authors
Cipollo JF, Trimble RB, Chi JH, Yan Q, Dean N.
  Title
The yeast ALG11 gene specifies addition of the terminal alpha 1,2-Man to the Man5GlcNAc2-PP-dolichol N-glycosylation intermediate formed on the cytosolic side of the endoplasmic reticulum.
  Journal
J Biol Chem 276:21828-40 (2001)
Reference
  Authors
Frank CG, Aebi M.
  Title
ALG9 mannosyltransferase is involved in two different steps of lipid-linked oligosaccharide biosynthesis.
  Journal
Glycobiology 15:1156-63 (2005)
Reference
  Authors
O'Reilly MK, Zhang G, Imperiali B.
  Title
In vitro evidence for the dual function of Alg2 and Alg11: essential mannosyltransferases in N-linked glycoprotein biosynthesis.
  Journal
Biochemistry 45:9593-603 (2006)
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