| Entry |
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| Name |
Glycosylphosphatidylinositol(GPI)-anchor biosynthesis - Leishmania braziliensis
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| Description |
Cell surface proteins can be attached to the cell membrane via the glycolipid structure called glycosylphosphatidylinositol (GPI) anchor. Hundreds of GPI-anchored proteins have been identified in many eukaryotes ranging from protozoa and fungi to mammals. All protein-linked GPI anchors share a common core structure, characterized by the substructure Man (a1-4) GlcN (a1-6) myo-inositol-1P-lipid. Biosynthesis of GPI anchors proceeds in three stages: (i) preassembly of a GPI precursor in the ER membrane, (ii) attachment of the GPI to the C-terminus of a newly synthesized protein in the lumen of the ER, and (iii) lipid remodeling and/or carbohydrate side-chain modifications in the ER and the Golgi. Defects of GPI anchor biosynthesis gene result in a genetic disorder, paroxysmal nocturnal hemoglobinuria.
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| Class |
Metabolism; Glycan biosynthesis and metabolism
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| Pathway map |
| Glycosylphosphatidylinositol(GPI)-anchor biosynthesis |
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| Module |
| GPI-anchor biosynthesis, core oligosaccharide [PATH: lbz00563] |
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| Other DBs |
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| Organism |
Leishmania braziliensis [GN: lbz]
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| Gene |
| UDP-GlcNAc:PI A1-6 GlcNAc-transferase; K03857 phosphatidylinositol glycan, class A [EC: 2.4.1.198] [KO: K03857] [EC: 2.4.1.198] | | putative phosphatidylinositolN-acetylglucosaminyltransfe ra sesubunit C; K03859 phosphatidylinositol glycan, class C [KO: K03859] | | hypothetical protein; K03861 phosphatidylinositol glycan, class P [KO: K03861] | | hypothetical protein; K09658 dolichyl-phosphate mannosyltransferase polypeptide 2, regulatory subunit [KO: K09658] | | N-acetyl-D-glucosaminylphosphatidylinositol de-N-acetylase; K03434 N-acetylglucosaminylphosphatidylinositol deacetylase [EC: 3.5.1.89] [KO: K03434] [EC: 3.5.1.89] | | GPI14; putative mannosyltransferase [KO: K05284] [EC:2.4.1.-] | | putative ethanolamine phosphotransferase; K05288 phosphatidylinositol glycan, class O [KO: K05288] | | GPI8; putative GPI-anchor transamidase subunit 8 (GPI8) [KO: K05290] [EC:2.6.-.-] |
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| Compound |
| Palmitoyl-CoA | | Phosphatidylethanolamine | | 1-Phosphatidyl-D-myo-inositol | | | | | | | | | | | | | | | | UDP-N-acetyl-D-glucosamine | | Dolichyl phosphate D-mannose | | 6-(alpha-D-glucosaminyl)-1D-myo-inositol | | | | | | |
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| Reference |
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| Authors |
Eisenhaber B, Maurer-Stroh S, Novatchkova M, Schneider G, Eisenhaber F. |
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| Title |
Enzymes and auxiliary factors for GPI lipid anchor biosynthesis and post-translational transfer to proteins. |
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| Journal |
Bioessays 25:367-85 (2003) |
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| Reference |
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| Authors |
Kinoshita T, Inoue N. |
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| Title |
Dissecting and manipulating the pathway for glycosylphos-phatidylinositol-anchor biosynthesis. |
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| Journal |
Curr Opin Chem Biol 4:632-8 (2000) |
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| Reference |
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| Authors |
Fankhauser C, Homans SW, Thomas-Oates JE, McConville MJ, Desponds C, Conzelmann A, Ferguson MA. |
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| Title |
Structures of glycosylphosphatidylinositol membrane anchors from Saccharomyces cerevisiae. |
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| Journal |
J Biol Chem 268:26365-74 (1993) |
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| Reference |
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| Authors |
Murakami Y, Siripanyapinyo U, Hong Y, Kang JY, Ishihara S, Nakakuma H, Maeda Y, Kinoshita T. |
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| Title |
PIG-W is critical for inositol acylation but not for flipping of glycosylphosphatidylinositol-anchor. |
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| Journal |
Mol Biol Cell 14:4285-95 (2003) |
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| Reference |
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| Authors |
Benachour A, Sipos G, Flury I, Reggiori F, Canivenc-Gansel E, Vionnet C, Conzelmann A, Benghezal M. |
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| Title |
Deletion of GPI7, a yeast gene required for addition of a side chain to the glycosylphosphatidylinositol (GPI) core structure, affects GPI protein transport, remodeling, and cell wall integrity. |
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| Journal |
J Biol Chem 274:15251-61 (1999) |
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| Reference |
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| Authors |
Tanaka S, Maeda Y, Tashima Y, Kinoshita T. |
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| Title |
Inositol deacylation of glycosylphosphatidylinositol-anchored proteins is mediated by mammalian PGAP1 and yeast Bst1p. |
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| Journal |
J Biol Chem 279:14256-63 (2004) |
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| Reference |
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| Authors |
Hong Y, Ohishi K, Kang JY, Tanaka S, Inoue N, Nishimura J, Maeda Y, Kinoshita T. |
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| Title |
Human PIG-U and yeast Cdc91p are the fifth subunit of GPI transamidase that attaches GPI-anchors to proteins. |
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| Journal |
Mol Biol Cell 14:1780-9 (2003) |
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| Reference |
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| Authors |
Tanaka S, Maeda Y, Kinoshita T. |
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| Title |
[Biosynthesis of GPI-anchored proteins] |
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| Journal |
Tanpakushitsu Kakusan Koso 48:990-6 (2003) |
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| Reference |
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| Authors |
Kang JY, Hong Y, Ashida H, Shishioh N, Murakami Y, Morita YS, Maeda Y, Kinoshita T. |
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| Title |
PIG-V involved in transferring the second mannose in glycosylphosphatidylinositol. |
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| Journal |
J Biol Chem 280:9489-97 (2005) |
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| Reference |
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| Authors |
Ashida H, Hong Y, Murakami Y, Shishioh N, Sugimoto N, Kim YU, Maeda Y, Kinoshita T. |
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| Title |
Mammalian PIG-X and yeast Pbn1p are the essential components of glycosylphosphatidylinositol-mannosyltransferase I. |
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| Journal |
Mol Biol Cell 16:1439-48 (2005) |
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| KO pathway |
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