Cell cycle - yeast - Neosartorya fischeri

Mitotic cell cycle progression is accomplished through a reproducible sequence of events, DNA replication (S phase) and mitosis (M phase) separated temporally by gaps (G1 and G2 phases). G1, S, and G2 phases are collectively known as interphase. In yeast Cdc28 is the catalytic subunit of the cyclin-dependent kinase (CDK). At G1 phase Cdc28 associates with G1-cyclins Cln1 to Cln3, while B-type cyclins Clb1 to Clb6 regulate Cdc28 during S, G2, and M phases. Cln3/Cdc28 activity is required for cells to pass through 'start', the commitment point in G1. When Cln3/Cdc28 accumulates more than a certain threshold, SBF (Swi4/Swi6) and MBF (Mbp1/Swi6) are activated, promoting transcription of Cln1, Cln2, and other genes required for S-phase progression. Cln1 and Cln2 interacting with Cdc28 promote activation of B-type cyclin associated CDK, which drives DNA replication and entry into mitosis. Specifically, Cdc28 association with Clb2 and Clb1 promotes entry into mitosis. Cells suffering from DNA damage, spindle misorientation, or spindle assembly defect do not undergo the metaphase-anaphase transition for chromosome segregation and fail to exit from mitosis. The spindle assembly checkpoint activates Mad2, which in turn prevents chromosome segregation by inhibiting degradation of the securin Pds1. Moreover, Pds1 is phosphorylated and stabilized in response to DNA damage in a Chk1-dependent manner. The spindle checkpoint is also involved in the Cdc14 release from the nucleolus. Cdc14 dephosphorylates Swi5, Sic1, and Cdh1, leading to inhibition of Cdc28 and degradation of cyclin required for mitotic exit.

Cellular Processes; Cell growth and death

Neosartorya fischeri [GN:nfi]

PMID:11967159

Devault A, Vallen EA, Yuan T, Green S, Bensimon A, Schwob E

Identification of Tah11/Sid2 as the ortholog of the replication licensing factor Cdt1 in Saccharomyces cerevisiae.

Curr Biol 12:689-94 (2002)

PMID:15210110

de Bruin RA, McDonald WH, Kalashnikova TI, Yates J 3rd, Wittenberg C

Cln3 activates G1-specific transcription via phosphorylation of the SBF bound repressor Whi5.

Cell 117:887-98 (2004)

PMID:15210111

Costanzo M, Nishikawa JL, Tang X, Millman JS, Schub O, Breitkreuz K, Dewar D, Rupes I, Andrews B, Tyers M

CDK activity antagonizes Whi5, an inhibitor of G1/S transcription in yeast.

Cell 117:899-913 (2004)

PMID:12464633

Pramila T, Miles S, GuhaThakurta D, Jemiolo D, Breeden LL

Conserved homeodomain proteins interact with MADS box protein Mcm1 to restrict ECB-dependent transcription to the M/G1 phase of the cell cycle.

Genes Dev 16:3034-45 (2002)

PMID:15060174

Schwickart M, Havlis J, Habermann B, Bogdanova A, Camasses A, Oelschlaegel T, Shevchenko A, Zachariae W

Swm1/Apc13 is an evolutionarily conserved subunit of the anaphase-promoting complex stabilizing the association of Cdc16 and Cdc27.

Mol Cell Biol 24:3562-76 (2004)

PMID:15797380

Penkner AM, Prinz S, Ferscha S, Klein F

Mnd2, an essential antagonist of the anaphase-promoting complex during meiotic prophase.

Cell 120:789-801 (2005)

PMID:18598214

Pesin JA, Orr-Weaver TL

Regulation of APC/C activators in mitosis and meiosis.

Annu Rev Cell Dev Biol 24:475-99 (2008)

PMID:7588610

Dirick L, Bohm T, Nasmyth K

Roles and regulation of Cln-Cdc28 kinases at the start of the cell cycle of Saccharomyces cerevisiae.

EMBO J 14:4803-13 (1995)

PMID:16285853

Bahler J

Cell-cycle control of gene expression in budding and fission yeast.

Annu Rev Genet 39:69-94 (2005)

PMID:8066461

Peter M, Herskowitz I

Direct inhibition of the yeast cyclin-dependent kinase Cdc28-Cln by Far1.

Science 265:1228-31 (1994)

PMID:9367986

Henchoz S, Chi Y, Catarin B, Herskowitz I, Deshaies RJ, Peter M

Phosphorylation- and ubiquitin-dependent degradation of the cyclin-dependent kinase inhibitor Far1p in budding yeast.

Genes Dev 11:3046-60 (1997)

PMID:9346239

Feldman RM, Correll CC, Kaplan KB, Deshaies RJ

A complex of Cdc4p, Skp1p, and Cdc53p/cullin catalyzes ubiquitination of the phosphorylated CDK inhibitor Sic1p.

Cell 91:221-30 (1997)

PMID:7954792

Schwob E, Bohm T, Mendenhall MD, Nasmyth K

The B-type cyclin kinase inhibitor p40SIC1 controls the G1 to S transition in S. cerevisiae.

Cell 79:233-44 (1994)

PMID:9841670

Mendenhall MD, Hodge AE

Regulation of Cdc28 cyclin-dependent protein kinase activity during the cell cycle of the yeast Saccharomyces cerevisiae.

Microbiol Mol Biol Rev 62:1191-243 (1998)

PMID:10465783

Zachariae W, Nasmyth K

Whose end is destruction: cell division and the anaphase-promoting complex.

Genes Dev 13:2039-58 (1999)

PMID:11269496

Jares P, Donaldson A, Blow JJ

The Cdc7/Dbf4 protein kinase: target of the S phase checkpoint?

EMBO Rep 1:319-22 (2000)

PMID:10512865

Elsasser S, Chi Y, Yang P, Campbell JL

Phosphorylation controls timing of Cdc6p destruction: A biochemical analysis.

Mol Biol Cell 10:3263-77 (1999)

PMID:10213692

Skowyra D, Koepp DM, Kamura T, Conrad MN, Conaway RC, Conaway JW, Elledge SJ, Harper JW

Reconstitution of G1 cyclin ubiquitination with complexes containing SCFGrr1 and Rbx1.

Science 284:662-5 (1999)

PMID:16487340

Mouillon JM, Persson BL

New aspects on phosphate sensing and signalling in Saccharomyces cerevisiae.

FEMS Yeast Res 6:171-6 (2006)

PMID:15965243

Bean JM, Siggia ED, Cross FR

High functional overlap between MluI cell-cycle box binding factor and Swi4/6 cell-cycle box binding factor in the G1/S transcriptional program in Saccharomyces cerevisiae.

Genetics 171:49-61 (2005)

PMID:9024629

Deshaies RJ

Phosphorylation and proteolysis: partners in the regulation of cell division in budding yeast.

Curr Opin Genet Dev 7:7-16 (1997)

PMID:15920482

Asano S, Park JE, Sakchaisri K, Yu LR, Song S, Supavilai P, Veenstra TD, Lee KS

Concerted mechanism of Swe1/Wee1 regulation by multiple kinases in budding yeast.

EMBO J 24:2194-204 (2005)

PMID:9716410

Kaiser P, Sia RA, Bardes EG, Lew DJ, Reed SI

Cdc34 and the F-box protein Met30 are required for degradation of the Cdk-inhibitory kinase Swe1.

Genes Dev 12:2587-97 (1998)

PMID:9925642

Barral Y, Parra M, Bidlingmaier S, Snyder M

Nim1-related kinases coordinate cell cycle progression with the organization of the peripheral cytoskeleton in yeast.

Genes Dev 13:176-87 (1999)

PMID:10469560

Taylor SS

Chromosome segregation: dual control ensures fidelity.

Curr Biol 9:R562-4 (1999)

PMID:14616062

Lew DJ, Burke DJ

The spindle assembly and spindle position checkpoints.

Annu Rev Genet 37:251-82 (2003)

PMID:14514882

Simanis V

Events at the end of mitosis in the budding and fission yeasts.

J Cell Sci 116:4263-75 (2003)

PMID:8131744

Toyn JH, Johnston LH

The Dbf2 and Dbf20 protein kinases of budding yeast are activated after the metaphase to anaphase cell cycle transition.

EMBO J 13:1103-13 (1994)

PMID:11146641

Amon A

Together until separin do us part.

Nat Cell Biol 3:E12-4 (2001)

PMID:10882066

Ciosk R, Shirayama M, Shevchenko A, Tanaka T, Toth A, Shevchenko A, Nasmyth K

Cohesin's binding to chromosomes depends on a separate complex consisting of Scc2 and Scc4 proteins.

Mol Cell 5:243-54 (2000)

PMID:10811823

Freeman L, Aragon-Alcaide L, Strunnikov A

The condensin complex governs chromosome condensation and mitotic transmission of rDNA.

J Cell Biol 149:811-24 (2000)

PMID:18616603

Fu Y, Pastushok L, Xiao W

DNA damage-induced gene expression in Saccharomyces cerevisiae.

FEMS Microbiol Rev 32:908-26 (2008)

PMID:19230642

Friedel AM, Pike BL, Gasser SM

ATR/Mec1: coordinating fork stability and repair.

Curr Opin Cell Biol 21:237-44 (2009)

PMID:19457865

Chen SH, Zhou H

Reconstitution of Rad53 activation by Mec1 through adaptor protein Mrc1.

J Biol Chem 284:18593-604 (2009)

PMID:19021755

Amon A

A decade of Cdc14--a personal perspective. Delivered on 9 July 2007 at the 32nd FEBS Congress in Vienna, Austria.

FEBS J 275:5774-84 (2008)