| Entry |
|
|---|
| Name |
Peptidoglycan biosynthesis - Pseudomonas entomophila
|
|---|
| Description |
Peptidoglycan is a macromolecule made of long aminosugar strands cross-linked by short peptides. It forms the cell wall in bacteria surrounding the cytoplasmic membrane. The glycan strands are typically comprised of repeating N-acetylglucosamine (GlcNAc) and N-acetylmuramic acid (MurNAc) disaccharides. Each MurNAc is linked to a peptide of three to five amino acid residues. Disaccharide subunits are first assembled on the cytoplasmic side of the bacterial membrane on a polyisoprenoid anchor (lipid I and II). Polymerization of disaccharide subunits by transglycosylases and cross-linking of glycan strands by transpeptidases occur on the other side of the membrane. Bacterial cell wall biosynthesis inhibitors form a major class of antibiotics.
|
|---|
| Class |
Metabolism; Glycan biosynthesis and metabolism
 |
|---|
| Pathway map |
| Peptidoglycan biosynthesis |
  |
|---|
| Organism |
Pseudomonas entomophila [GN: pen]
|
|---|
| Gene |
| murA; UDP-N-acetylglucosamine 1-carboxyvinyltransferase [KO: K00790] [EC: 2.5.1.7] | | murB; UDP-N-acetylenolpyruvoylglucosamine reductase [KO: K00075] [EC: 1.3.1.98] | | murC; UDP-N-acetylmuramate--L-alanine ligase [KO: K01924] [EC: 6.3.2.8] | | murD; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [KO: K01925] [EC: 6.3.2.9] | | murE; UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase [KO: K01928] [EC: 6.3.2.13] | | | | murF; UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase [KO: K01929] [EC: 6.3.2.10] | | uppP; undecaprenyl pyrophosphate phosphatase [KO: K06153] [EC: 3.6.1.27] | | mraY; phospho-N-acetylmuramoyl-pentapeptide-transferase [KO: K01000] [EC: 2.7.8.13] | | murG; undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase [KO: K02563] [EC: 2.4.1.227] | | virulence factor MviN family protein; K03980 virulence factor [KO: K03980] | | mtgA; monofunctional biosynthetic peptidoglycan transglycosylase [KO: K03814] [EC:2.4.1.-] | | pbpC; penicillin-binding protein 1C [KO: K05367] [EC:2.4.1.-] | | mrcA; penicillin-binding protein 1A [KO: K05366] [EC:3.4.-.- 2.4.1.-] | | | | penicillin-binding protein 2; K05515 penicillin-binding protein 2 [KO: K05515] | | pbpA; penicillin-binding protein 2 [KO: K05515] | | | | | | dacB; D-alanyl-D-alanine carboxypeptidase [KO: K07259] [EC:3.4.21.- 3.4.16.4] |
|
|---|
| Compound |
| Orthophosphate | | UDP-N-acetyl-alpha-D-glucosamine | | UMP | | D-Alanine | | UDP-N-acetylmuramoyl-L-alanyl-D-glutamate | | D-Alanyl-D-alanine | | UDP-N-acetylmuramate | | UDP-N-acetylmuramoyl-L-alanine | | di-trans,poly-cis-Undecaprenyl diphosphate | | UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine | | UDPMurAc(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) | | MurAc(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphospho-undecaprenol | | UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminopimelate | | UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-6-carboxy-L-lysyl-D-alanyl-D-alanine | | UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine | | Undecaprenyl-diphospho-N-acetylmuramoyl-(N-acetylglucosamine)-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine | | Undecaprenyl-diphospho-N-acetylmuramoyl-(N-acetylglucosamine)-L-alanyl-D-isoglutaminyl-L-lysyl-D-alanyl-D-alanine | | Undecaprenyl-diphospho-N-acetylmuramoyl-(N-acetylglucosamine)-L-alanyl-D-isoglutaminyl-L-lysyl-(glycyl)5-D-alanyl-D-alanine | | Crosslinked peptideglycan | | Undecaprenyl-diphospho-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine | | Undecaprenyl-diphospho-N-acetylmuramoyl-(N-acetylglucosamine)-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine | | Undecaprenyl-diphospho-N-acetylmuramoyl-(N-acetylglucosamine)-L-alanyl-D-isoglutaminyl-L-lysyl-(glycyl)-D-alanyl-D-alanine | | Undecaprenyl-diphospho-N-acetylmuramoyl-(N-acetylglucosamine)-L-alanyl-D-isoglutaminyl-L-lysyl-(glycyl)3-D-alanyl-D-alanine | | Undecaprenyl-diphospho-N-acetylmuramoyl-(N-acetylglucosamine)-L-alanyl-gamma-D-glutamyl-L-lysyl-(L-alanyl-L-alanyl)-D-alanyl-D-alanine | | Undecaprenyl-diphospho-N-acetylmuramoyl-(N-acetylglucosamine)-L-alanyl-gamma-D-glutamyl-L-lysyl-(L-alanyl)-D-alanyl-D-alanine | | di-trans,poly-cis-Undecaprenyl phosphate | | di-trans,poly-cis-Undecaprenol |
|
|---|
| Reference |
|
|---|
| Authors |
Bouhss A, Trunkfield AE, Bugg TD, Mengin-Lecreulx D |
|---|
| Title |
The biosynthesis of peptidoglycan lipid-linked intermediates. |
|---|
| Journal |
FEMS Microbiol Rev 32:208-33 (2008) |
|---|
| Reference |
|
|---|
| Authors |
Lloyd AJ, Gilbey AM, Blewett AM, De Pascale G, El Zoeiby A, Levesque RC, Catherwood AC, Tomasz A, Bugg TD, Roper DI, Dowson CG |
|---|
| Title |
Characterization of tRNA-dependent peptide bond formation by MurM in the synthesis of Streptococcus pneumoniae peptidoglycan. |
|---|
| Journal |
J Biol Chem 283:6402-17 (2008) |
|---|
| Reference |
|
|---|
| Authors |
Barreteau H, Kovac A, Boniface A, Sova M, Gobec S, Blanot D |
|---|
| Title |
Cytoplasmic steps of peptidoglycan biosynthesis. |
|---|
| Journal |
FEMS Microbiol Rev 32:168-207 (2008) |
|---|
| Reference |
|
|---|
| Authors |
van Heijenoort J |
|---|
| Title |
Recent advances in the formation of the bacterial peptidoglycan monomer unit. |
|---|
| Journal |
Nat Prod Rep 18:503-19 (2001) |
|---|
| Reference |
|
|---|
| Authors |
van Heijenoort J |
|---|
| Title |
Formation of the glycan chains in the synthesis of bacterial peptidoglycan. |
|---|
| Journal |
Glycobiology 11:25R-36R (2001) |
|---|
| KO pathway |
|
|---|
