Phosphotransferase system (PTS) - Salmonella enterica subsp. enterica serovar Typhimurium UK-1

The phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS) is a major mechanism used by bacteria for uptake of carbohydrates, particularly hexoses, hexitols, and disaccharides, where the source of energy is from PEP. The PTS consists of two general components, enzyme I (EI) and histidine phosphocarrier protein (HPr), and of membrane-bound sugar specific permeases (enzymes II). Each enzyme II (EII) complex consists of one or two hydrophobic integral membrane domains (domains C and D) and two hydrophilic domains (domains A and B). EII complexes may exist as distinct proteins or as a single multidomain protein. The PTS catalyzes the uptake of carbohydrates and their conversion into their respective phosphoesters during transport. There are four successive phosphoryl transfers in the PTS. Initial autophosphorylation of EI, using PEP as a substrate, is followed by transfer of the phosphoryl group from EI to HPr. EIIA catalyzes the self-phosphoryl transfer from HPr after which the phosphoryl group is transferred to histidine or cysteine residues of EIIB. The sugar is transported through the membrane-bound EIIC and is phosphorylated by the appropriate sugar-specific EIIB.

Environmental Information Processing; Membrane transport

Salmonella enterica subsp. enterica serovar Typhimurium UK-1 [GN:sej]

PMID:11361076

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J Mol Microbiol Biotechnol 3:439-44 (2001)

PMID:15060041

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J Bacteriol 186:2385-92 (2004)

PMID:2117666

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PMID:12603740

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Mol Microbiol 47:1353-66 (2003)

PMID:14645248

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Phosphotransferase-mediated transport of the osmolyte 2-O-alpha-mannosyl-D-glycerate in Escherichia coli occurs by the product of the mngA (hrsA) gene and is regulated by the mngR (farR) gene product acting as repressor.

J Biol Chem 279:5537-48 (2004)

PMID:15608210

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Nucleic Acids Res 33:D334-7 (2005)

PMID:18156259

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J Bacteriol 190:1710-7 (2008)

PMID:10931310

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Mol Microbiol 37:125-35 (2000)

PMID:11361063

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J Mol Microbiol Biotechnol 3:329-46 (2001)

PMID:16233138

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Bacterial phosphotransferase system (PTS) in carbohydrate uptake and control of carbon metabolism.

J Biosci Bioeng 92:502-17 (2001)