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DNA replication - Sphingobium japonicum
A complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (DnaB or MCM complex) precedes the DNA synthetic machinery and unwinds the duplex parental DNA in cooperation with the SSB or RPA. On the leading strand, replication occurs continuously in a 5 to 3 direction, whereas on the lagging strand, DNA replication occurs discontinuously by synthesis and joining of short Okazaki fragments. In prokaryotes, the leading strand replication apparatus consists of a DNA polymerase (pol III core), a sliding clamp (beta), and a clamp loader (gamma delta complex). The DNA primase (DnaG) is needed to form RNA primers. Normally, during replication of the lagging-strand DNA template, an RNA primer is removed either by an RNase H or by the 5 to 3 exonuclease activity of DNA pol I, and the DNA ligase joins the Okazaki fragments. In eukaryotes, three DNA polymerases (alpha, delta, and epsilon) have been identified. DNA primase forms a permanent complex with DNA polymerase alpha. PCNA and RFC function as a clamp and a clamp loader. FEN 1 and RNase H1 remove the RNA from the Okazaki fragments and DNA ligase I joins the DNA.
Genetic Information Processing; Replication and repair
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DNA replication

All organismsOrtholog table
DNA polymerase III complex, bacteria [PATH:sjp03030]
Sphingobium japonicum [GN:sjp]
dnaQ; DNA polymerase III epsilon subunit [KO:K02342] [EC:]
dnaE; DNA polymerase III alpha subunit [KO:K02337] [EC:]
dnaX; DNA polymerase III gamma and tau subunit [KO:K02343] [EC:]
holB; DNA polymerase III delta' subunit [KO:K02341] [EC:]
DNA polymerase III delta subunit [KO:K02340] [EC:]
DNA polymerase III chi subunit [KO:K02339] [EC:]
dnaN; DNA polymerase III beta subunit [KO:K02338] [EC:]
dnaB; replicative DNA helicase [KO:K02314] [EC:]
dnaG; DNA primase [KO:K02316] [EC:2.7.7.-]
single-stranded DNA-binding protein [KO:K03111]
ssb; single-stranded DNA-binding protein [KO:K03111]
ssb; single-stranded DNA-binding protein [KO:K03111]
rnhA; ribonuclease HI [KO:K03469] [EC:]
rnhB; ribonuclease HII [KO:K03470] [EC:]
dpo1; DNA polymerase I [KO:K02335] [EC:]
ligA; NAD-dependent DNA ligase [KO:K01972] [EC:]
lig; ATP-dependent DNA ligase [KO:K10747] [EC:]
Stillman B.
Smart machines at the DNA replication fork.
Cell 78:725-8 (1994)
Waga S, Stillman B.
The DNA replication fork in eukaryotic cells.
Annu Rev Biochem 67:721-51 (1998)
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