GenomeNet

Database: PDB
Entry: 1E0N
LinkDB: 1E0N
Original site: 1E0N 
HEADER    YJQ8WW DOMAIN                           01-APR-00   1E0N              
TITLE     YJQ8WW DOMAIN FROM SACCHAROMYCES CEREVISAE                            
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: HYPOTHETICAL PROTEIN;                                      
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: DOMAIN;                                                    
COMPND   5 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: SACCHAROMYCES CEREVISIAE;                       
SOURCE   3 ORGANISM_TAXID: 4932;                                                
SOURCE   4 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   5 EXPRESSION_SYSTEM_TAXID: 562                                         
KEYWDS    YJQ8WW DOMAIN, WW DOMAIN, SACCHAROMYCES CEREVISAE, YJQ8               
KEYWDS   2 PROTEIN                                                              
EXPDTA    SOLUTION NMR                                                          
NUMMDL    10                                                                    
AUTHOR    M.J.MACIAS,V.GERVAIS,C.CIVERA,H.OSCHKINAT                             
REVDAT   3   24-FEB-09 1E0N    1       VERSN                                    
REVDAT   2   09-JUN-00 1E0N    1       ENDMDL                                   
REVDAT   1   02-JUN-00 1E0N    0                                                
JRNL        AUTH   M.J.MACIAS,V.GERVAIS,C.CIVERA,H.OSCHKINAT                    
JRNL        TITL   STRUCTURAL ANALYSIS OF WW DOMAINS AND DESIGN OF A            
JRNL        TITL 2 WW PROTOTYPE                                                 
JRNL        REF    NAT.STRUCT.BIOL.              V.   7   375 2000              
JRNL        REFN                   ISSN 1072-8368                               
JRNL        PMID   10802733                                                     
JRNL        DOI    10.1038/75144                                                
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : ARIA/CNS                                             
REMARK   3   AUTHORS     : 1. NILGES, M.                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 1E0N COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON  03-APR-00.                 
REMARK 100 THE PDBE ID CODE IS EBI-4807.                                        
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 285                                
REMARK 210  PH                             : 6.5                                
REMARK 210  IONIC STRENGTH                 : NULL                               
REMARK 210  PRESSURE                       : NULL                               
REMARK 210  SAMPLE CONTENTS                : NULL                               
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : NOESY; TOCSY                       
REMARK 210  SPECTROMETER FIELD STRENGTH    : 600                                
REMARK 210  SPECTROMETER MODEL             : DRX 600                            
REMARK 210  SPECTROMETER MANUFACTURER      : BRUKER                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : XEASY/ARIA/CNS                     
REMARK 210   METHOD USED                   : SIMULATED ANNEALING                
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 20                                 
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 10                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : LOWEST ENERGY                      
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: THE STRUCTURE WAS DETERMINED USING HOMONUCLEAR NMR           
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 GLU A  10     -152.40   -142.28                                   
REMARK 500  1 GLU A  14      -68.03    -96.67                                   
REMARK 500  1 ASN A  22      119.45   -172.36                                   
REMARK 500  1 LEU A  29     -173.94   -177.66                                   
REMARK 500  2 GLU A  10     -157.62   -138.20                                   
REMARK 500  2 ASN A  22      123.75   -175.34                                   
REMARK 500  3 GLU A  10     -157.08   -135.04                                   
REMARK 500  3 PRO A  18       90.85    -62.12                                   
REMARK 500  3 LEU A  29     -176.43    176.57                                   
REMARK 500  3 TYR A  31      147.32   -171.02                                   
REMARK 500  4 GLU A  10     -156.09   -153.35                                   
REMARK 500  4 ASN A  22      130.40   -172.08                                   
REMARK 500  4 LYS A  26       82.50     75.88                                   
REMARK 500  4 PRO A  32      166.73    -47.91                                   
REMARK 500  5 PRO A  18      138.65    -32.20                                   
REMARK 500  5 ASN A  22      119.40   -173.51                                   
REMARK 500  5 PRO A  32      168.90    -49.13                                   
REMARK 500  6 GLU A  10     -155.84   -145.84                                   
REMARK 500  6 ILE A  12     -156.40   -136.44                                   
REMARK 500  6 ASN A  22      124.28   -172.78                                   
REMARK 500  7 GLU A  10     -154.38   -136.35                                   
REMARK 500  7 PRO A  18       96.32    -63.94                                   
REMARK 500  7 ASN A  22      120.01   -177.36                                   
REMARK 500  7 LEU A  29     -170.40    178.37                                   
REMARK 500  7 HIS A  30      -51.57   -126.54                                   
REMARK 500  8 GLU A  10     -159.45   -137.82                                   
REMARK 500  8 PRO A  18       90.31    -65.18                                   
REMARK 500  8 ASN A  22      126.64   -172.58                                   
REMARK 500  8 LYS A  26       71.31     59.80                                   
REMARK 500  8 LEU A  29     -171.99   -175.86                                   
REMARK 500  9 GLU A  10     -159.71   -138.06                                   
REMARK 500  9 GLU A  14      -57.11   -123.55                                   
REMARK 500  9 PRO A  18       93.98    -66.83                                   
REMARK 500  9 ASN A  22      120.52   -175.25                                   
REMARK 500  9 LEU A  29     -167.79   -178.78                                   
REMARK 500  9 HIS A  30      -50.86   -133.04                                   
REMARK 500 10 GLU A  10     -152.55   -134.95                                   
REMARK 500 10 PRO A  18       98.56    -66.01                                   
REMARK 500 10 ASN A  22      120.62   -175.17                                   
REMARK 500 10 LEU A  29      171.12    179.26                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 1E0M   RELATED DB: PDB                                   
REMARK 900  PROTOTYPE WW DOMAIN                                                 
REMARK 900 RELATED ID: 1E0L   RELATED DB: PDB                                   
REMARK 900  FBP28WW DOMAIN FROM MUS MUSCULUS                                    
REMARK 900 RELATED ID: 4715   RELATED DB: BMRB                                  
REMARK 900  NMR RESTRAINTS FOR ENTRY 1E0N                                       
DBREF  1E0N A    7    33  PDB    1E0N     1E0N             7     33             
SEQRES   1 A   27  PRO GLY TRP GLU ILE ILE HIS GLU ASN GLY ARG PRO LEU          
SEQRES   2 A   27  TYR TYR ASN ALA GLU GLN LYS THR LYS LEU HIS TYR PRO          
SEQRES   3 A   27  PRO                                                          
SHEET    1   A 3 TRP A   9  GLU A  14  0                                        
SHEET    2   A 3 ARG A  17  ALA A  23 -1  O  LEU A  19   N  ILE A  12           
SHEET    3   A 3 LYS A  26  HIS A  30 -1  O  THR A  27   N  ASN A  22           
CISPEP   1 PRO A   32    PRO A   33          7         1.86                     
CISPEP   2 TYR A   31    PRO A   32          9        -1.85                     
CISPEP   3 TYR A   31    PRO A   32         10        -3.11                     
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
DBGET integrated database retrieval system