HEADER PRION PROTEIN 17-SEP-99 1QLX
TITLE HUMAN PRION PROTEIN
COMPND MOL_ID: 1;
COMPND 2 MOLECULE: PRION PROTEIN;
COMPND 3 CHAIN: A;
COMPND 4 FRAGMENT: RESIDUES 23-230;
COMPND 5 SYNONYM: PRP, MAJOR PRION PROTEIN, PRP27-30, PRP33-35C,
COMPND 6 (ASCR).PRP;
COMPND 7 ENGINEERED: YES;
COMPND 8 MUTATION: YES
SOURCE MOL_ID: 1;
SOURCE 2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;
SOURCE 3 ORGANISM_COMMON: HUMAN;
SOURCE 4 ORGANISM_TAXID: 9606;
SOURCE 5 ORGAN: BRAIN;
SOURCE 6 CELLULAR_LOCATION: EXTRACELLULAR;
SOURCE 7 GENE: PRNP;
SOURCE 8 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
SOURCE 9 EXPRESSION_SYSTEM_TAXID: 469008;
SOURCE 10 EXPRESSION_SYSTEM_STRAIN: BL21(DE3);
SOURCE 11 EXPRESSION_SYSTEM_CELLULAR_LOCATION: CYTOPLASM;
SOURCE 12 EXPRESSION_SYSTEM_PLASMID: PRSET A
KEYWDS PRION PROTEIN, PRION, BRAIN, GLYCOPROTEIN, GPI-ANCHOR,
KEYWDS 2 REPEAT, POLYMORPHISM, DISEASE MUTATION
EXPDTA SOLUTION NMR
MDLTYP MINIMIZED AVERAGE
AUTHOR R.ZAHN,A.LIU,T.LUHRS,K.WUTHRICH
REVDAT 3 24-FEB-09 1QLX 1 VERSN
REVDAT 2 26-FEB-02 1QLX 1 JRNL REMARK
REVDAT 1 16-DEC-99 1QLX 0
JRNL AUTH R.ZAHN,A.LIU,T.LUHRS,R.RIEK,C.VON SCHROETTER,
JRNL AUTH 2 F.L.GARCIA,M.BILLETER,L.CALZOLAI,G.WIDER,K.WUTHRICH
JRNL TITL NMR SOLUTION STRUCTURE OF THE HUMAN PRION PROTEIN
JRNL REF PROC.NATL.ACAD.SCI.USA V. 97 145 2000
JRNL REFN ISSN 0027-8424
JRNL PMID 10618385
JRNL DOI 10.1073/PNAS.97.1.145
REMARK 2
REMARK 2 RESOLUTION. NOT APPLICABLE.
REMARK 3
REMARK 3 REFINEMENT.
REMARK 3 PROGRAM : OPALP
REMARK 3 AUTHORS : KORADI, BILLETER, GUNTERT
REMARK 3
REMARK 3 OTHER REFINEMENT REMARKS: NULL
REMARK 4
REMARK 4 1QLX COMPLIES WITH FORMAT V. 3.15, 01-DEC-08
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON 17-SEP-99.
REMARK 100 THE PDBE ID CODE IS EBI-4014.
REMARK 210
REMARK 210 EXPERIMENTAL DETAILS
REMARK 210 EXPERIMENT TYPE : NMR
REMARK 210 TEMPERATURE (KELVIN) : 293
REMARK 210 PH : 4.5
REMARK 210 IONIC STRENGTH : 0.01
REMARK 210 PRESSURE : 1 ATM
REMARK 210 SAMPLE CONTENTS : 90% WATER/10% D2O
REMARK 210
REMARK 210 NMR EXPERIMENTS CONDUCTED : NOESY; COSY
REMARK 210 SPECTROMETER FIELD STRENGTH : 750
REMARK 210 SPECTROMETER MODEL : DRX750
REMARK 210 SPECTROMETER MANUFACTURER : BRUKER
REMARK 210
REMARK 210 STRUCTURE DETERMINATION.
REMARK 210 SOFTWARE USED : DYANA
REMARK 210 METHOD USED : TORSION ANGLE DYNAMICS
REMARK 210
REMARK 210 CONFORMERS, NUMBER CALCULATED : 100
REMARK 210 CONFORMERS, NUMBER SUBMITTED : 1
REMARK 210 CONFORMERS, SELECTION CRITERIA : LEAST RESTRAINT VIOLATION
REMARK 210
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : NULL
REMARK 210
REMARK 210 REMARK: CLOSEST TO THE MEAN (HEAVY ATOMS OF RESIDUES 125-228).
REMARK 210 THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR
REMARK 210 SPECTROSCOPY ON 13C, 15N-LABELED PROTEIN
REMARK 215
REMARK 215 NMR STUDY
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION
REMARK 215 NMR DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE THAT
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON
REMARK 215 THESE RECORDS ARE MEANINGLESS.
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465 M RES C SSSEQI
REMARK 465 GLY A 21
REMARK 465 SER A 22
REMARK 465 LYS A 23
REMARK 465 LYS A 24
REMARK 465 ARG A 25
REMARK 465 PRO A 26
REMARK 465 LYS A 27
REMARK 465 PRO A 28
REMARK 465 GLY A 29
REMARK 465 GLY A 30
REMARK 465 TRP A 31
REMARK 465 ASN A 32
REMARK 465 THR A 33
REMARK 465 GLY A 34
REMARK 465 GLY A 35
REMARK 465 SER A 36
REMARK 465 ARG A 37
REMARK 465 TYR A 38
REMARK 465 PRO A 39
REMARK 465 GLY A 40
REMARK 465 GLN A 41
REMARK 465 GLY A 42
REMARK 465 SER A 43
REMARK 465 PRO A 44
REMARK 465 GLY A 45
REMARK 465 GLY A 46
REMARK 465 ASN A 47
REMARK 465 ARG A 48
REMARK 465 TYR A 49
REMARK 465 PRO A 50
REMARK 465 PRO A 51
REMARK 465 GLN A 52
REMARK 465 GLY A 53
REMARK 465 GLY A 54
REMARK 465 GLY A 55
REMARK 465 GLY A 56
REMARK 465 TRP A 57
REMARK 465 GLY A 58
REMARK 465 GLN A 59
REMARK 465 PRO A 60
REMARK 465 HIS A 61
REMARK 465 GLY A 62
REMARK 465 GLY A 63
REMARK 465 GLY A 64
REMARK 465 TRP A 65
REMARK 465 GLY A 66
REMARK 465 GLN A 67
REMARK 465 PRO A 68
REMARK 465 HIS A 69
REMARK 465 GLY A 70
REMARK 465 GLY A 71
REMARK 465 GLY A 72
REMARK 465 TRP A 73
REMARK 465 GLY A 74
REMARK 465 GLN A 75
REMARK 465 PRO A 76
REMARK 465 HIS A 77
REMARK 465 GLY A 78
REMARK 465 GLY A 79
REMARK 465 GLY A 80
REMARK 465 TRP A 81
REMARK 465 GLY A 82
REMARK 465 GLN A 83
REMARK 465 PRO A 84
REMARK 465 HIS A 85
REMARK 465 GLY A 86
REMARK 465 GLY A 87
REMARK 465 GLY A 88
REMARK 465 TRP A 89
REMARK 465 GLY A 90
REMARK 465 GLN A 91
REMARK 465 GLY A 92
REMARK 465 GLY A 93
REMARK 465 GLY A 94
REMARK 465 THR A 95
REMARK 465 HIS A 96
REMARK 465 SER A 97
REMARK 465 GLN A 98
REMARK 465 TRP A 99
REMARK 465 ASN A 100
REMARK 465 LYS A 101
REMARK 465 PRO A 102
REMARK 465 SER A 103
REMARK 465 LYS A 104
REMARK 465 PRO A 105
REMARK 465 LYS A 106
REMARK 465 THR A 107
REMARK 465 ASN A 108
REMARK 465 MET A 109
REMARK 465 LYS A 110
REMARK 465 HIS A 111
REMARK 465 MET A 112
REMARK 465 ALA A 113
REMARK 465 GLY A 114
REMARK 465 ALA A 115
REMARK 465 ALA A 116
REMARK 465 ALA A 117
REMARK 465 ALA A 118
REMARK 465 GLY A 119
REMARK 465 ALA A 120
REMARK 465 VAL A 121
REMARK 465 VAL A 122
REMARK 465 GLY A 123
REMARK 465 GLY A 124
REMARK 465 GLY A 229
REMARK 465 SER A 230
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500 M RES CSSEQI PSI PHI
REMARK 500 ASP A 167 -79.47 -70.83
REMARK 500 GLU A 168 -35.99 -144.82
REMARK 500 SER A 170 -97.36 33.87
REMARK 500 ASN A 171 151.81 64.02
REMARK 500 GLN A 172 -99.99 -87.50
REMARK 500 THR A 188 -7.52 -58.94
REMARK 500 VAL A 189 -73.11 -110.27
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: PLANAR GROUPS
REMARK 500
REMARK 500 PLANAR GROUPS IN THE FOLLOWING RESIDUES HAVE A TOTAL
REMARK 500 RMS DISTANCE OF ALL ATOMS FROM THE BEST-FIT PLANE
REMARK 500 BY MORE THAN AN EXPECTED VALUE OF 6*RMSD, WITH AN
REMARK 500 RMSD 0.02 ANGSTROMS, OR AT LEAST ONE ATOM HAS
REMARK 500 AN RMSD GREATER THAN THIS VALUE
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 M RES CSSEQI RMS TYPE
REMARK 500 TYR A 145 0.07 SIDE CHAIN
REMARK 500 ARG A 228 0.11 SIDE CHAIN
REMARK 500
REMARK 500 REMARK: NULL
REMARK 650
REMARK 650 HELIX
REMARK 650 DETERMINATION METHOD: AUTHOR PROVIDED.
REMARK 700
REMARK 700 SHEET
REMARK 700 DETERMINATION METHOD: AUTHOR PROVIDED.
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: 1AG2 RELATED DB: PDB
REMARK 900 PRION PROTEIN DOMAIN PRP(121-231) FROM MOUSE, NMR,
REMARK 900 MINIMIZED AVERAGE STRUCTURE
REMARK 900 RELATED ID: 1QLZ RELATED DB: PDB
REMARK 900 HUMAN PRION PROTEIN NMR, 20 STRUCTURES, RESIDUES 23-230
REMARK 900 RELATED ID: 1QM0 RELATED DB: PDB
REMARK 900 HUMAN PRION PROTEIN FRAGMENT 90-230, NMR, REPRESENTATIVE
REMARK 900 STRUCTURE
REMARK 900 RELATED ID: 1QM1 RELATED DB: PDB
REMARK 900 HUMAN PRION PROTEIN FRAGMENT 90-230, NMR, 20 STRUCTURES
REMARK 900 RELATED ID: 1QM2 RELATED DB: PDB
REMARK 900 HUMAN PRION PROTEIN FRAGMENT 121-230, NMR, REPRESENTATIVE
REMARK 900 STRUCTURE
REMARK 900 RELATED ID: 1QM3 RELATED DB: PDB
REMARK 900 HUMAN PRION PROTEIN FRAGMENT 121-230, NMR, 20 STRUCTURES
REMARK 999
REMARK 999 SEQUENCE
REMARK 999 2 RESIDUES (GLY SER) INSERTED AT THE N-TERMINUS
DBREF 1QLX A 23 230 UNP P04156 PRIO_HUMAN 23 230
SEQADV 1QLX GLY A 21 UNP P04156 CLONING ARTIFACT
SEQADV 1QLX SER A 22 UNP P04156 CLONING ARTIFACT
SEQRES 1 A 210 GLY SER LYS LYS ARG PRO LYS PRO GLY GLY TRP ASN THR
SEQRES 2 A 210 GLY GLY SER ARG TYR PRO GLY GLN GLY SER PRO GLY GLY
SEQRES 3 A 210 ASN ARG TYR PRO PRO GLN GLY GLY GLY GLY TRP GLY GLN
SEQRES 4 A 210 PRO HIS GLY GLY GLY TRP GLY GLN PRO HIS GLY GLY GLY
SEQRES 5 A 210 TRP GLY GLN PRO HIS GLY GLY GLY TRP GLY GLN PRO HIS
SEQRES 6 A 210 GLY GLY GLY TRP GLY GLN GLY GLY GLY THR HIS SER GLN
SEQRES 7 A 210 TRP ASN LYS PRO SER LYS PRO LYS THR ASN MET LYS HIS
SEQRES 8 A 210 MET ALA GLY ALA ALA ALA ALA GLY ALA VAL VAL GLY GLY
SEQRES 9 A 210 LEU GLY GLY TYR MET LEU GLY SER ALA MET SER ARG PRO
SEQRES 10 A 210 ILE ILE HIS PHE GLY SER ASP TYR GLU ASP ARG TYR TYR
SEQRES 11 A 210 ARG GLU ASN MET HIS ARG TYR PRO ASN GLN VAL TYR TYR
SEQRES 12 A 210 ARG PRO MET ASP GLU TYR SER ASN GLN ASN ASN PHE VAL
SEQRES 13 A 210 HIS ASP CYS VAL ASN ILE THR ILE LYS GLN HIS THR VAL
SEQRES 14 A 210 THR THR THR THR LYS GLY GLU ASN PHE THR GLU THR ASP
SEQRES 15 A 210 VAL LYS MET MET GLU ARG VAL VAL GLU GLN MET CYS ILE
SEQRES 16 A 210 THR GLN TYR GLU ARG GLU SER GLN ALA TYR TYR GLN ARG
SEQRES 17 A 210 GLY SER
HELIX 1 H1 ASP A 144 MET A 154 1 11
HELIX 2 H2 ASN A 173 LYS A 194 1 22
HELIX 3 H3 GLU A 200 ARG A 228 1 29
SHEET 1 S1 2 TYR A 128 GLY A 131 0
SHEET 2 S1 2 VAL A 161 ARG A 164 -1 N TYR A 128 O VAL A 161
SSBOND 1 CYS A 179 CYS A 214 1555 1555 2.02
CRYST1 1.000 1.000 1.000 90.00 90.00 90.00 P 1 1
ORIGX1 1.000000 0.000000 0.000000 0.00000
ORIGX2 0.000000 1.000000 0.000000 0.00000
ORIGX3 0.000000 0.000000 1.000000 0.00000
SCALE1 1.000000 0.000000 0.000000 0.00000
SCALE2 0.000000 1.000000 0.000000 0.00000
SCALE3 0.000000 0.000000 1.000000 0.00000
(ATOM LINES ARE NOT SHOWN.)
END
