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Database: PDB
Entry: 1SNT
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Original site: 1SNT 
HEADER    HYDROLASE                               12-MAR-04   1SNT              
TITLE     STRUCTURE OF THE HUMAN CYTOSOLIC SIALIDASE NEU2                       
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: SIALIDASE 2;                                               
COMPND   3 CHAIN: A;                                                            
COMPND   4 SYNONYM: NEU2, CYTOSOLIC SIALIDASE, N-ACETYL-ALPHA- NEURAMINIDASE 2; 
COMPND   5 EC: 3.2.1.18;                                                        
COMPND   6 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21;                            
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 511693;                                     
SOURCE   7 EXPRESSION_SYSTEM_STRAIN: BL21;                                      
SOURCE   8 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE   9 EXPRESSION_SYSTEM_PLASMID: PGEX-2T                                   
KEYWDS    SIALIDASE, NEURAMINIDASE, GANGLIOSIDE, HYDROLASE                      
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    L.M.G.CHAVAS,P.FUSI,C.TRINGALI,B.VENERANDO,G.TETTAMANTI,R.KATO,       
AUTHOR   2 E.MONTI,S.WAKATSUKI                                                  
REVDAT   4   13-JUL-11 1SNT    1       VERSN                                    
REVDAT   3   24-FEB-09 1SNT    1       VERSN                                    
REVDAT   2   18-JAN-05 1SNT    1       JRNL                                     
REVDAT   1   02-NOV-04 1SNT    0                                                
JRNL        AUTH   L.M.G.CHAVAS,C.TRINGALI,P.FUSI,B.VENERANDO,G.TETTAMANTI,     
JRNL        AUTH 2 R.KATO,E.MONTI,S.WAKATSUKI                                   
JRNL        TITL   CRYSTAL STRUCTURE OF THE HUMAN CYTOSOLIC SIALIDASE NEU2:     
JRNL        TITL 2 EVIDENCE FOR THE DYNAMIC NATURE OF SUBSTRATE RECOGNITION     
JRNL        REF    J.BIOL.CHEM.                  V. 280   469 2005              
JRNL        REFN                   ISSN 0021-9258                               
JRNL        PMID   15501818                                                     
JRNL        DOI    10.1074/JBC.M411506200                                       
REMARK   2                                                                      
REMARK   2 RESOLUTION.    1.75 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : CNS                                                  
REMARK   3   AUTHORS     : BRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE-              
REMARK   3               : KUNSTLEVE,JIANG,KUSZEWSKI,NILGES,PANNU,              
REMARK   3               : READ,RICE,SIMONSON,WARREN                            
REMARK   3                                                                      
REMARK   3  REFINEMENT TARGET : ENGH & HUBER                                    
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.75                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 40.00                          
REMARK   3   DATA CUTOFF            (SIGMA(F)) : 0.000                          
REMARK   3   DATA CUTOFF HIGH         (ABS(F)) : NULL                           
REMARK   3   DATA CUTOFF LOW          (ABS(F)) : NULL                           
REMARK   3   COMPLETENESS (WORKING+TEST)   (%) : 99.5                           
REMARK   3   NUMBER OF REFLECTIONS             : 51297                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   CROSS-VALIDATION METHOD          : THROUGHOUT                      
REMARK   3   FREE R VALUE TEST SET SELECTION  : RANDOM                          
REMARK   3   R VALUE            (WORKING SET) : 0.201                           
REMARK   3   FREE R VALUE                     : 0.236                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : NULL                            
REMARK   3   FREE R VALUE TEST SET COUNT      : 2551                            
REMARK   3   ESTIMATED ERROR OF FREE R VALUE  : NULL                            
REMARK   3                                                                      
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.                                  
REMARK   3   TOTAL NUMBER OF BINS USED           : NULL                         
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : NULL                         
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : NULL                         
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : NULL                         
REMARK   3   REFLECTIONS IN BIN    (WORKING SET) : NULL                         
REMARK   3   BIN R VALUE           (WORKING SET) : NULL                         
REMARK   3   BIN FREE R VALUE                    : NULL                         
REMARK   3   BIN FREE R VALUE TEST SET SIZE  (%) : NULL                         
REMARK   3   BIN FREE R VALUE TEST SET COUNT     : NULL                         
REMARK   3   ESTIMATED ERROR OF BIN FREE R VALUE : NULL                         
REMARK   3                                                                      
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.                    
REMARK   3   PROTEIN ATOMS            : 2768                                    
REMARK   3   NUCLEIC ACID ATOMS       : 0                                       
REMARK   3   HETEROGEN ATOMS          : 0                                       
REMARK   3   SOLVENT ATOMS            : 377                                     
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL                           
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  ESTIMATED COORDINATE ERROR.                                         
REMARK   3   ESD FROM LUZZATI PLOT        (A) : NULL                            
REMARK   3   ESD FROM SIGMAA              (A) : NULL                            
REMARK   3   LOW RESOLUTION CUTOFF        (A) : NULL                            
REMARK   3                                                                      
REMARK   3  CROSS-VALIDATED ESTIMATED COORDINATE ERROR.                         
REMARK   3   ESD FROM C-V LUZZATI PLOT    (A) : NULL                            
REMARK   3   ESD FROM C-V SIGMAA          (A) : NULL                            
REMARK   3                                                                      
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES.                                   
REMARK   3   BOND LENGTHS                 (A) : 0.006                           
REMARK   3   BOND ANGLES            (DEGREES) : 1.47                            
REMARK   3   DIHEDRAL ANGLES        (DEGREES) : NULL                            
REMARK   3   IMPROPER ANGLES        (DEGREES) : NULL                            
REMARK   3                                                                      
REMARK   3  ISOTROPIC THERMAL MODEL : NULL                                      
REMARK   3                                                                      
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.    RMS    SIGMA                
REMARK   3   MAIN-CHAIN BOND              (A**2) : NULL  ; NULL                 
REMARK   3   MAIN-CHAIN ANGLE             (A**2) : NULL  ; NULL                 
REMARK   3   SIDE-CHAIN BOND              (A**2) : NULL  ; NULL                 
REMARK   3   SIDE-CHAIN ANGLE             (A**2) : NULL  ; NULL                 
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELING.                                              
REMARK   3   METHOD USED : NULL                                                 
REMARK   3   KSOL        : NULL                                                 
REMARK   3   BSOL        : NULL                                                 
REMARK   3                                                                      
REMARK   3  NCS MODEL : NULL                                                    
REMARK   3                                                                      
REMARK   3  NCS RESTRAINTS.                         RMS   SIGMA/WEIGHT          
REMARK   3   GROUP  1  POSITIONAL            (A) : NULL  ; NULL                 
REMARK   3   GROUP  1  B-FACTOR           (A**2) : NULL  ; NULL                 
REMARK   3                                                                      
REMARK   3  PARAMETER FILE  1  : NULL                                           
REMARK   3  TOPOLOGY FILE  1   : NULL                                           
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS:                                           
REMARK   3  ABOUT CD1/CD2 (LEU A 90) AND CG1/CG2 (VAL A 325),                   
REMARK   3  THERE ARE 3 POSSIBLE POSITIONS FOR THESE ATOMS.                     
REMARK   3  THERE ARE TWO DIFFERENT CONFORMATIONS FOR EACH ATOM.                
REMARK   3  FOR CONVENIENCE, THE AUTHOR ASSIGNED AS A DOUBLE                    
REMARK   3  CONFORMATION ONLY ONE OF THE ATOMS.(CD2 OF LEU 90                   
REMARK   3  AND CG1 OF VAL 325)                                                 
REMARK   3  THUS THE OCCUPANCIES OF THE ALTERNATE CONFORMATIONS                 
REMARK   3  ARE GREATER THAN 1.00 AND THERE ARE CHIRALITY ERRORS                
REMARK   3  AT THESE ATOMS.                                                     
REMARK   4                                                                      
REMARK   4 1SNT COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 16-MAR-04.                  
REMARK 100 THE RCSB ID CODE IS RCSB021858.                                      
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 24-MAY-03                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 6.2                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : PHOTON FACTORY                     
REMARK 200  BEAMLINE                       : AR-NW12A                           
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.9780                             
REMARK 200  MONOCHROMATOR                  : SI(111)                            
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : ADSC QUANTUM 210                   
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : HKL-2000                           
REMARK 200  DATA SCALING SOFTWARE          : SCALEPACK                          
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 51297                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.750                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 40.000                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.5                               
REMARK 200  DATA REDUNDANCY                : NULL                               
REMARK 200  R MERGE                    (I) : 0.07200                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 5.5000                             
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.75                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 1.84                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 99.5                               
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL                               
REMARK 200  R MERGE FOR SHELL          (I) : 0.38300                            
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 2.000                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: MOLREP                                                
REMARK 200 STARTING MODEL: PDB ENTRY 1EUS                                       
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 58.35                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.98                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: SODIUM POTASSIUM PHOSPHATE, SODIUM       
REMARK 280  CHLORIDE, PH 6.2, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 289K   
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: H 3                              
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -Y,X-Y,Z                                                
REMARK 290       3555   -X+Y,-X,Z                                               
REMARK 290       4555   X+2/3,Y+1/3,Z+1/3                                       
REMARK 290       5555   -Y+2/3,X-Y+1/3,Z+1/3                                    
REMARK 290       6555   -X+Y+2/3,-X+1/3,Z+1/3                                   
REMARK 290       7555   X+1/3,Y+2/3,Z+2/3                                       
REMARK 290       8555   -Y+1/3,X-Y+2/3,Z+2/3                                    
REMARK 290       9555   -X+Y+1/3,-X+2/3,Z+2/3                                   
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -0.500000 -0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.866025 -0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   3 -0.500000  0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   3 -0.866025 -0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000       72.97000            
REMARK 290   SMTRY2   4  0.000000  1.000000  0.000000       42.12925            
REMARK 290   SMTRY3   4  0.000000  0.000000  1.000000       21.45000            
REMARK 290   SMTRY1   5 -0.500000 -0.866025  0.000000       72.97000            
REMARK 290   SMTRY2   5  0.866025 -0.500000  0.000000       42.12925            
REMARK 290   SMTRY3   5  0.000000  0.000000  1.000000       21.45000            
REMARK 290   SMTRY1   6 -0.500000  0.866025  0.000000       72.97000            
REMARK 290   SMTRY2   6 -0.866025 -0.500000  0.000000       42.12925            
REMARK 290   SMTRY3   6  0.000000  0.000000  1.000000       21.45000            
REMARK 290   SMTRY1   7  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   7  0.000000  1.000000  0.000000       84.25850            
REMARK 290   SMTRY3   7  0.000000  0.000000  1.000000       42.90000            
REMARK 290   SMTRY1   8 -0.500000 -0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   8  0.866025 -0.500000  0.000000       84.25850            
REMARK 290   SMTRY3   8  0.000000  0.000000  1.000000       42.90000            
REMARK 290   SMTRY1   9 -0.500000  0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   9 -0.866025 -0.500000  0.000000       84.25850            
REMARK 290   SMTRY3   9  0.000000  0.000000  1.000000       42.90000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A                                     
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     GLY A    -1                                                      
REMARK 465     SER A     0                                                      
REMARK 465     MET A     1                                                      
REMARK 465     ALA A     2                                                      
REMARK 465     ALA A    42                                                      
REMARK 465     SER A    43                                                      
REMARK 465     LYS A    44                                                      
REMARK 465     LYS A    45                                                      
REMARK 465     ASP A    46                                                      
REMARK 465     GLU A    47                                                      
REMARK 465     HIS A    48                                                      
REMARK 465     GLY A   107                                                      
REMARK 465     GLN A   108                                                      
REMARK 465     VAL A   109                                                      
REMARK 465     THR A   110                                                      
REMARK 465     GLU A   111                                                      
REMARK 465     GLN A   112                                                      
REMARK 465     GLN A   113                                                      
REMARK 465     GLN A   114                                                      
REMARK 465     LEU A   115                                                      
REMARK 465     GLN A   116                                                      
REMARK 465     THR A   117                                                      
REMARK 465     ARG A   118                                                      
REMARK 465     SER A   284                                                      
REMARK 465     GLY A   285                                                      
REMARK 465     PRO A   286                                                      
REMARK 465     GLY A   287                                                      
REMARK 465     LEU A   378                                                      
REMARK 465     PRO A   379                                                      
REMARK 465     GLN A   380                                                      
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    ILE A  22       69.39     60.93                                   
REMARK 500    LEU A 184        3.78    -68.31                                   
REMARK 500    PRO A 186       37.69    -75.90                                   
REMARK 500    VAL A 212     -159.70    -94.75                                   
REMARK 500    LEU A 217     -147.78   -119.00                                   
REMARK 500    GLU A 218      117.14    -31.66                                   
REMARK 500    LEU A 240     -163.97   -100.65                                   
REMARK 500    ASP A 306       76.90     58.58                                   
REMARK 500    ALA A 333     -122.50   -127.75                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 525                                                                      
REMARK 525 SOLVENT                                                              
REMARK 525                                                                      
REMARK 525 THE SOLVENT MOLECULES HAVE CHAIN IDENTIFIERS THAT                    
REMARK 525 INDICATE THE POLYMER CHAIN WITH WHICH THEY ARE MOST                  
REMARK 525 CLOSELY ASSOCIATED. THE REMARK LISTS ALL THE SOLVENT                 
REMARK 525 MOLECULES WHICH ARE MORE THAN 5A AWAY FROM THE                       
REMARK 525 NEAREST POLYMER CHAIN (M = MODEL NUMBER;                             
REMARK 525 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE                  
REMARK 525 NUMBER; I=INSERTION CODE):                                           
REMARK 525                                                                      
REMARK 525  M RES CSSEQI                                                        
REMARK 525    HOH A 386        DISTANCE =  5.37 ANGSTROMS                       
REMARK 525    HOH A 404        DISTANCE =  6.85 ANGSTROMS                       
REMARK 525    HOH A 596        DISTANCE =  5.12 ANGSTROMS                       
REMARK 525    HOH A 637        DISTANCE =  6.65 ANGSTROMS                       
REMARK 525    HOH A 654        DISTANCE =  5.61 ANGSTROMS                       
REMARK 525    HOH A 656        DISTANCE =  5.12 ANGSTROMS                       
REMARK 525    HOH A 678        DISTANCE =  5.04 ANGSTROMS                       
REMARK 525    HOH A 716        DISTANCE =  6.40 ANGSTROMS                       
REMARK 525    HOH A 731        DISTANCE =  5.96 ANGSTROMS                       
REMARK 525    HOH A 732        DISTANCE =  6.17 ANGSTROMS                       
REMARK 525    HOH A 749        DISTANCE =  5.83 ANGSTROMS                       
REMARK 525    HOH A 750        DISTANCE =  5.40 ANGSTROMS                       
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 1VCU   RELATED DB: PDB                                   
REMARK 900 THE SAME PROTEIN COMPLEXED WITH SIALIC ACID                          
REMARK 900 RELATED ID: 1SO7   RELATED DB: PDB                                   
REMARK 900 THE SAME PROTEIN, SUGAR-INDUCED FORM                                 
DBREF  1SNT A    1   380  UNP    Q9Y3R4   NEUR2_HUMAN      1    380             
SEQADV 1SNT GLY A   -1  UNP  Q9Y3R4              CLONING ARTIFACT               
SEQADV 1SNT SER A    0  UNP  Q9Y3R4              CLONING ARTIFACT               
SEQRES   1 A  382  GLY SER MET ALA SER LEU PRO VAL LEU GLN LYS GLU SER          
SEQRES   2 A  382  VAL PHE GLN SER GLY ALA HIS ALA TYR ARG ILE PRO ALA          
SEQRES   3 A  382  LEU LEU TYR LEU PRO GLY GLN GLN SER LEU LEU ALA PHE          
SEQRES   4 A  382  ALA GLU GLN ARG ALA SER LYS LYS ASP GLU HIS ALA GLU          
SEQRES   5 A  382  LEU ILE VAL LEU ARG ARG GLY ASP TYR ASP ALA PRO THR          
SEQRES   6 A  382  HIS GLN VAL GLN TRP GLN ALA GLN GLU VAL VAL ALA GLN          
SEQRES   7 A  382  ALA ARG LEU ASP GLY HIS ARG SER MET ASN PRO CYS PRO          
SEQRES   8 A  382  LEU TYR ASP ALA GLN THR GLY THR LEU PHE LEU PHE PHE          
SEQRES   9 A  382  ILE ALA ILE PRO GLY GLN VAL THR GLU GLN GLN GLN LEU          
SEQRES  10 A  382  GLN THR ARG ALA ASN VAL THR ARG LEU CYS GLN VAL THR          
SEQRES  11 A  382  SER THR ASP HIS GLY ARG THR TRP SER SER PRO ARG ASP          
SEQRES  12 A  382  LEU THR ASP ALA ALA ILE GLY PRO ALA TYR ARG GLU TRP          
SEQRES  13 A  382  SER THR PHE ALA VAL GLY PRO GLY HIS CYS LEU GLN LEU          
SEQRES  14 A  382  ASN ASP ARG ALA ARG SER LEU VAL VAL PRO ALA TYR ALA          
SEQRES  15 A  382  TYR ARG LYS LEU HIS PRO ILE GLN ARG PRO ILE PRO SER          
SEQRES  16 A  382  ALA PHE CYS PHE LEU SER HIS ASP HIS GLY ARG THR TRP          
SEQRES  17 A  382  ALA ARG GLY HIS PHE VAL ALA GLN ASP THR LEU GLU CYS          
SEQRES  18 A  382  GLN VAL ALA GLU VAL GLU THR GLY GLU GLN ARG VAL VAL          
SEQRES  19 A  382  THR LEU ASN ALA ARG SER HIS LEU ARG ALA ARG VAL GLN          
SEQRES  20 A  382  ALA GLN SER THR ASN ASP GLY LEU ASP PHE GLN GLU SER          
SEQRES  21 A  382  GLN LEU VAL LYS LYS LEU VAL GLU PRO PRO PRO GLN GLY          
SEQRES  22 A  382  CYS GLN GLY SER VAL ILE SER PHE PRO SER PRO ARG SER          
SEQRES  23 A  382  GLY PRO GLY SER PRO ALA GLN TRP LEU LEU TYR THR HIS          
SEQRES  24 A  382  PRO THR HIS SER TRP GLN ARG ALA ASP LEU GLY ALA TYR          
SEQRES  25 A  382  LEU ASN PRO ARG PRO PRO ALA PRO GLU ALA TRP SER GLU          
SEQRES  26 A  382  PRO VAL LEU LEU ALA LYS GLY SER CYS ALA TYR SER ASP          
SEQRES  27 A  382  LEU GLN SER MET GLY THR GLY PRO ASP GLY SER PRO LEU          
SEQRES  28 A  382  PHE GLY CYS LEU TYR GLU ALA ASN ASP TYR GLU GLU ILE          
SEQRES  29 A  382  VAL PHE LEU MET PHE THR LEU LYS GLN ALA PHE PRO ALA          
SEQRES  30 A  382  GLU TYR LEU PRO GLN                                          
FORMUL   2  HOH   *377(H2 O)                                                    
HELIX    1   1 LEU A  142  GLY A  148  1                                   7    
HELIX    2   2 PRO A  149  ARG A  152  5                                   4    
HELIX    3   3 ALA A  317  TRP A  321  5                                   5    
HELIX    4   4 LEU A  369  PHE A  373  1                                   5    
HELIX    5   5 PRO A  374  TYR A  377  5                                   4    
SHEET    1   A 7 GLN A   8  GLN A  14  0                                        
SHEET    2   A 7 GLU A 361  THR A 368 -1  O  PHE A 364   N  GLU A  10           
SHEET    3   A 7 PRO A 348  ALA A 356 -1  N  TYR A 354   O  VAL A 363           
SHEET    4   A 7 VAL A 325  THR A 342 -1  N  MET A 340   O  LEU A 349           
SHEET    5   A 7 ALA A 305  ASN A 312 -1  N  LEU A 307   O  LEU A 327           
SHEET    6   A 7 GLN A 291  PRO A 298 -1  N  TYR A 295   O  TYR A 310           
SHEET    7   A 7 SER A 275  PRO A 280 -1  N  PHE A 279   O  TRP A 292           
SHEET    1   B 4 TYR A  20  LEU A  28  0                                        
SHEET    2   B 4 SER A  33  GLN A  40 -1  O  GLU A  39   N  ARG A  21           
SHEET    3   B 4 LEU A  51  ASP A  60 -1  O  GLY A  57   N  LEU A  34           
SHEET    4   B 4 GLN A  65  TRP A  68 -1  O  GLN A  65   N  ASP A  60           
SHEET    1   C 4 TYR A  20  LEU A  28  0                                        
SHEET    2   C 4 SER A  33  GLN A  40 -1  O  GLU A  39   N  ARG A  21           
SHEET    3   C 4 LEU A  51  ASP A  60 -1  O  GLY A  57   N  LEU A  34           
SHEET    4   C 4 GLU A  72  VAL A  73 -1  O  GLU A  72   N  LEU A  54           
SHEET    1   D 4 ARG A  83  TYR A  91  0                                        
SHEET    2   D 4 LEU A  98  ILE A 105 -1  O  PHE A  99   N  LEU A  90           
SHEET    3   D 4 ARG A 123  SER A 129 -1  O  CYS A 125   N  PHE A 102           
SHEET    4   D 4 ARG A 140  ASP A 141 -1  O  ARG A 140   N  GLN A 126           
SHEET    1   E 3 TRP A 154  VAL A 159  0                                        
SHEET    2   E 3 LEU A 174  ARG A 182 -1  O  TYR A 181   N  THR A 156           
SHEET    3   E 3 LEU A 165  GLN A 166 -1  N  LEU A 165   O  VAL A 175           
SHEET    1   F 4 TRP A 154  VAL A 159  0                                        
SHEET    2   F 4 LEU A 174  ARG A 182 -1  O  TYR A 181   N  THR A 156           
SHEET    3   F 4 ILE A 191  SER A 199 -1  O  SER A 199   N  LEU A 174           
SHEET    4   F 4 ALA A 207  ARG A 208 -1  O  ALA A 207   N  LEU A 198           
SHEET    1   G 4 THR A 216  THR A 226  0                                        
SHEET    2   G 4 GLN A 229  SER A 238 -1  O  THR A 233   N  ALA A 222           
SHEET    3   G 4 ALA A 242  SER A 248 -1  O  VAL A 244   N  ALA A 236           
SHEET    4   G 4 GLN A 259  VAL A 265 -1  O  GLN A 259   N  GLN A 245           
SSBOND   1 CYS A   88    CYS A  164                          1555   1555  2.83  
CISPEP   1 GLY A  160    PRO A  161          0         0.43                     
CISPEP   2 PRO A  268    PRO A  269          0         0.13                     
CISPEP   3 PRO A  315    PRO A  316          0        -0.12                     
CRYST1  145.940  145.940   64.350  90.00  90.00 120.00 H 3           9          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.006852  0.003956  0.000000        0.00000                         
SCALE2      0.000000  0.007912  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.015540        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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