HEADER STRUCTURAL GENOMICS, UNKNOWN FUNCTION 28-MAY-04 1WI6
TITLE SOLUTION STRUCTURE OF THE RNA BINDING DOMAIN FROM MOUSE HYPOTHETICAL
TITLE 2 PROTEIN BAB23670
COMPND MOL_ID: 1;
COMPND 2 MOLECULE: HYPOTHETICAL PROTEIN (RIKEN CDNA 1300006N24);
COMPND 3 CHAIN: A;
COMPND 4 FRAGMENT: RNA RECOGNITION MOTIF;
COMPND 5 ENGINEERED: YES
SOURCE MOL_ID: 1;
SOURCE 2 ORGANISM_SCIENTIFIC: MUS MUSCULUS;
SOURCE 3 ORGANISM_COMMON: HOUSE MOUSE;
SOURCE 4 ORGANISM_TAXID: 10090;
SOURCE 5 GENE: RIKEN CDNA 1300006N24;
SOURCE 6 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;
SOURCE 7 EXPRESSION_SYSTEM_PLASMID: P031222-76;
SOURCE 8 OTHER_DETAILS: CELL-FREE PROTEIN SYNTHESIS
KEYWDS RNA RECOGNITION MOTIF, RRM, RNA BINDING DOMAIN, RBD, RNP, STRUCTURAL
KEYWDS 2 GENOMICS, RIKEN STRUCTURAL GENOMICS/PROTEOMICS INITIATIVE, RSGI,
KEYWDS 3 UNKNOWN FUNCTION
EXPDTA SOLUTION NMR
NUMMDL 20
AUTHOR S.SUZUKI,Y.MUTO,T.NAGATA,M.INOUE,T.KIGAWA,T.TERADA,M.SHIROUZU,
AUTHOR 2 S.YOKOYAMA,RIKEN STRUCTURAL GENOMICS/PROTEOMICS INITIATIVE (RSGI)
REVDAT 3 02-MAR-22 1WI6 1 REMARK SEQADV SHEET
REVDAT 2 24-FEB-09 1WI6 1 VERSN
REVDAT 1 07-JUN-05 1WI6 0
JRNL AUTH S.SUZUKI,Y.MUTO,T.NAGATA,M.INOUE,T.KIGAWA,T.TERADA,
JRNL AUTH 2 M.SHIROUZU,S.YOKOYAMA
JRNL TITL SOLUTION STRUCTURE OF THE RNA BINDING DOMAIN FROM MOUSE
JRNL TITL 2 HYPOTHETICAL PROTEIN BAB23670
JRNL REF TO BE PUBLISHED
JRNL REFN
REMARK 2
REMARK 2 RESOLUTION. NOT APPLICABLE.
REMARK 3
REMARK 3 REFINEMENT.
REMARK 3 PROGRAM : XWINNMR 3.5, CYANA 2.0.17
REMARK 3 AUTHORS : BRUKER (XWINNMR), GUENTERT, P. (CYANA)
REMARK 3
REMARK 3 OTHER REFINEMENT REMARKS: NULL
REMARK 4
REMARK 4 1WI6 COMPLIES WITH FORMAT V. 3.15, 01-DEC-08
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 04-JUN-04.
REMARK 100 THE DEPOSITION ID IS D_1000023605.
REMARK 210
REMARK 210 EXPERIMENTAL DETAILS
REMARK 210 EXPERIMENT TYPE : NMR
REMARK 210 TEMPERATURE (KELVIN) : 298
REMARK 210 PH : 7.0
REMARK 210 IONIC STRENGTH : 100MM
REMARK 210 PRESSURE : AMBIENT
REMARK 210 SAMPLE CONTENTS : 1.2MM 13C/15N-PROTEIN 20MM D
REMARK 210 -TRIS-HCL; 100MM NACL; 1MM D-DTT;
REMARK 210 0.02% NAN3
REMARK 210
REMARK 210 NMR EXPERIMENTS CONDUCTED : 3D_15N-SEPARATED_NOESY; 3D_13C
REMARK 210 -SEPARATED_NOESY
REMARK 210 SPECTROMETER FIELD STRENGTH : 700 MHZ
REMARK 210 SPECTROMETER MODEL : AVANCE
REMARK 210 SPECTROMETER MANUFACTURER : BRUKER
REMARK 210
REMARK 210 STRUCTURE DETERMINATION.
REMARK 210 SOFTWARE USED : NMRPIPE 2.3, NMRVIEW 5.0.4,
REMARK 210 KUJIRA 0.901, CYANA 2.0.17,
REMARK 210 OLIVIA 1.9.12
REMARK 210 METHOD USED : TORSION ANGLE DYNAMICS,
REMARK 210 SIMULATED ANNEALING
REMARK 210
REMARK 210 CONFORMERS, NUMBER CALCULATED : 100
REMARK 210 CONFORMERS, NUMBER SUBMITTED : 20
REMARK 210 CONFORMERS, SELECTION CRITERIA : TARGET FUNCTION
REMARK 210
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1
REMARK 210
REMARK 210 REMARK: THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR
REMARK 210 SPECTROSCOPY.
REMARK 215
REMARK 215 NMR STUDY
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION
REMARK 215 NMR DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE THAT
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON
REMARK 215 THESE RECORDS ARE MEANINGLESS.
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW. BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000
REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000
REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500 M RES CSSEQI PSI PHI
REMARK 500 2 LEU A 109 -71.76 -72.20
REMARK 500 2 GLN A 123 33.04 34.66
REMARK 500 2 ASP A 139 49.17 -95.69
REMARK 500 2 PRO A 146 0.15 -69.70
REMARK 500 3 SER A 63 136.38 -170.27
REMARK 500 3 GLN A 123 25.00 43.66
REMARK 500 3 ARG A 127 36.82 38.95
REMARK 500 3 LEU A 141 121.09 -172.51
REMARK 500 3 PRO A 146 1.67 -69.74
REMARK 500 3 SER A 147 100.14 -34.28
REMARK 500 4 GLN A 123 25.69 43.75
REMARK 500 4 LEU A 141 145.66 -172.03
REMARK 500 4 CYS A 143 39.88 -89.19
REMARK 500 5 SER A 67 76.06 -105.00
REMARK 500 5 VAL A 78 108.74 -59.61
REMARK 500 5 LEU A 109 -71.70 -62.45
REMARK 500 5 GLN A 123 28.18 45.57
REMARK 500 5 ARG A 127 40.53 36.25
REMARK 500 6 GLN A 123 25.91 43.81
REMARK 500 6 THR A 138 89.62 -58.83
REMARK 500 6 SER A 144 165.68 -45.24
REMARK 500 6 PRO A 146 87.62 -69.77
REMARK 500 7 SER A 66 -50.60 -129.16
REMARK 500 7 PRO A 75 -173.46 -69.79
REMARK 500 7 VAL A 78 108.78 -57.14
REMARK 500 7 LEU A 109 -72.82 -64.30
REMARK 500 7 GLN A 123 35.20 33.89
REMARK 500 8 GLN A 123 27.73 42.04
REMARK 500 8 ARG A 127 42.38 36.72
REMARK 500 8 LEU A 142 41.93 -97.09
REMARK 500 9 SER A 67 -63.57 -105.12
REMARK 500 9 LEU A 109 -71.75 -68.77
REMARK 500 9 GLN A 123 26.72 47.19
REMARK 500 9 THR A 138 157.26 -44.73
REMARK 500 9 PRO A 146 93.46 -69.72
REMARK 500 10 VAL A 78 109.52 -46.18
REMARK 500 10 LEU A 109 -73.41 -73.70
REMARK 500 10 GLN A 123 24.99 42.29
REMARK 500 10 ARG A 127 41.39 39.55
REMARK 500 10 ASP A 139 46.22 39.26
REMARK 500 11 SER A 64 107.70 -52.31
REMARK 500 11 VAL A 78 105.80 -55.79
REMARK 500 11 LEU A 109 -72.13 -70.67
REMARK 500 11 GLN A 123 27.60 45.90
REMARK 500 11 ARG A 127 38.91 38.51
REMARK 500 11 PRO A 137 96.31 -69.81
REMARK 500 11 THR A 138 150.88 -37.70
REMARK 500 12 LEU A 109 -74.14 -58.49
REMARK 500 12 GLN A 123 26.04 46.17
REMARK 500 12 ARG A 127 38.80 38.80
REMARK 500
REMARK 500 THIS ENTRY HAS 74 RAMACHANDRAN OUTLIERS.
REMARK 500
REMARK 500 REMARK: NULL
REMARK 650
REMARK 650 HELIX
REMARK 650 DETERMIANTION METHOD: AUTHOR DETERMINED
REMARK 700
REMARK 700 SHEET
REMARK 700 DETERMINATION METHOD: AUTHOR DETERMINED
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: MMT007006895 RELATED DB: TARGETDB
DBREF 1WI6 A 69 143 UNP Q8C3Z1 Q8C3Z1_MOUSE 69 143
SEQADV 1WI6 GLY A 62 UNP Q8C3Z1 CLONING ARTIFACT
SEQADV 1WI6 SER A 63 UNP Q8C3Z1 CLONING ARTIFACT
SEQADV 1WI6 SER A 64 UNP Q8C3Z1 CLONING ARTIFACT
SEQADV 1WI6 GLY A 65 UNP Q8C3Z1 CLONING ARTIFACT
SEQADV 1WI6 SER A 66 UNP Q8C3Z1 CLONING ARTIFACT
SEQADV 1WI6 SER A 67 UNP Q8C3Z1 CLONING ARTIFACT
SEQADV 1WI6 GLY A 68 UNP Q8C3Z1 CLONING ARTIFACT
SEQADV 1WI6 SER A 144 UNP Q8C3Z1 CLONING ARTIFACT
SEQADV 1WI6 GLY A 145 UNP Q8C3Z1 CLONING ARTIFACT
SEQADV 1WI6 PRO A 146 UNP Q8C3Z1 CLONING ARTIFACT
SEQADV 1WI6 SER A 147 UNP Q8C3Z1 CLONING ARTIFACT
SEQADV 1WI6 SER A 148 UNP Q8C3Z1 CLONING ARTIFACT
SEQADV 1WI6 GLY A 149 UNP Q8C3Z1 CLONING ARTIFACT
SEQRES 1 A 88 GLY SER SER GLY SER SER GLY ILE LEU ILE ARG GLY LEU
SEQRES 2 A 88 PRO GLY ASP VAL THR ASN GLN GLU VAL HIS ASP LEU LEU
SEQRES 3 A 88 SER ASP TYR GLU LEU LYS TYR CYS PHE VAL ASP LYS TYR
SEQRES 4 A 88 LYS GLY THR ALA PHE VAL THR LEU LEU ASN GLY GLU GLN
SEQRES 5 A 88 ALA GLU ALA ALA ILE ASN THR PHE HIS GLN SER ARG LEU
SEQRES 6 A 88 ARG GLU ARG GLU LEU SER VAL GLN LEU GLN PRO THR ASP
SEQRES 7 A 88 ALA LEU LEU CYS SER GLY PRO SER SER GLY
HELIX 1 1 ASN A 80 LEU A 86 1 7
HELIX 2 2 GLU A 112 HIS A 122 1 11
SHEET 1 A 6 ILE A 69 ARG A 72 0
SHEET 2 A 6 TYR A 94 ASP A 98 0
SHEET 3 A 6 THR A 103 LEU A 108 0
SHEET 4 A 6 SER A 124 LEU A 126 0
SHEET 5 A 6 ARG A 129 LEU A 131 0
SHEET 6 A 6 SER A 132 LEU A 135 0
CRYST1 1.000 1.000 1.000 90.00 90.00 90.00 P 1 1
ORIGX1 1.000000 0.000000 0.000000 0.00000
ORIGX2 0.000000 1.000000 0.000000 0.00000
ORIGX3 0.000000 0.000000 1.000000 0.00000
SCALE1 1.000000 0.000000 0.000000 0.00000
SCALE2 0.000000 1.000000 0.000000 0.00000
SCALE3 0.000000 0.000000 1.000000 0.00000
MODEL 1
(ATOM LINES ARE NOT SHOWN.)
END
