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Database: PDB
Entry: 1Z56
LinkDB: 1Z56
Original site: 1Z56 
HEADER    LIGASE                                  17-MAR-05   1Z56              
TITLE     CO-CRYSTAL STRUCTURE OF LIF1P-LIG4P                                   
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: LIGASE INTERACTING FACTOR 1;                               
COMPND   3 CHAIN: A, B;                                                         
COMPND   4 ENGINEERED: YES;                                                     
COMPND   5 MOL_ID: 2;                                                           
COMPND   6 MOLECULE: DNA LIGASE IV;                                             
COMPND   7 CHAIN: C;                                                            
COMPND   8 SYNONYM: POLYDEOXYRIBONUCLEOTIDE SYNTHASE [ATP], DNA                 
COMPND   9 LIGASE IV HOMOLOG;                                                   
COMPND  10 EC: 6.5.1.1;                                                         
COMPND  11 ENGINEERED: YES;                                                     
COMPND  12 MOL_ID: 3;                                                           
COMPND  13 MOLECULE: LIGASE INTERACTING FACTOR 1;                               
COMPND  14 CHAIN: D;                                                            
COMPND  15 FRAGMENT: UNCHARACTERIZED FRAGMENT;                                  
COMPND  16 ENGINEERED: YES;                                                     
COMPND  17 MOL_ID: 4;                                                           
COMPND  18 MOLECULE: LIGASE INTERACTING FACTOR 1;                               
COMPND  19 CHAIN: E, H;                                                         
COMPND  20 FRAGMENT: UNCHARACTERIZED FRAGMENT;                                  
COMPND  21 ENGINEERED: YES;                                                     
COMPND  22 MOL_ID: 5;                                                           
COMPND  23 MOLECULE: LIGASE INTERACTING FACTOR 1;                               
COMPND  24 CHAIN: F;                                                            
COMPND  25 FRAGMENT: UNCHARACTERIZED FRAGMENT;                                  
COMPND  26 ENGINEERED: YES;                                                     
COMPND  27 MOL_ID: 6;                                                           
COMPND  28 MOLECULE: LIGASE INTERACTING FACTOR 1;                               
COMPND  29 CHAIN: G;                                                            
COMPND  30 FRAGMENT: UNCHARACTERIZED FRAGMENT;                                  
COMPND  31 ENGINEERED: YES;                                                     
COMPND  32 MOL_ID: 7;                                                           
COMPND  33 MOLECULE: LIGASE INTERACTING FACTOR 1;                               
COMPND  34 CHAIN: I;                                                            
COMPND  35 FRAGMENT: UNCHARACTERIZED FRAGMENT;                                  
COMPND  36 ENGINEERED: YES;                                                     
COMPND  37 MOL_ID: 8;                                                           
COMPND  38 MOLECULE: LIGASE INTERACTING FACTOR 1;                               
COMPND  39 CHAIN: J;                                                            
COMPND  40 FRAGMENT: UNCHARACTERIZED FRAGMENT;                                  
COMPND  41 ENGINEERED: YES;                                                     
COMPND  42 MOL_ID: 9;                                                           
COMPND  43 MOLECULE: LIGASE INTERACTING FACTOR 1;                               
COMPND  44 CHAIN: K;                                                            
COMPND  45 FRAGMENT: UNCHARACTERIZED FRAGMENT;                                  
COMPND  46 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: SACCHAROMYCES CEREVISIAE;                       
SOURCE   3 ORGANISM_COMMON: BAKER'S YEAST;                                      
SOURCE   4 ORGANISM_TAXID: 4932;                                                
SOURCE   5 GENE: LIF1;                                                          
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21;                            
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 511693;                                     
SOURCE   8 EXPRESSION_SYSTEM_STRAIN: BL21;                                      
SOURCE   9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  10 EXPRESSION_SYSTEM_PLASMID: PET28A+;                                  
SOURCE  11 MOL_ID: 2;                                                           
SOURCE  12 ORGANISM_SCIENTIFIC: SACCHAROMYCES CEREVISIAE;                       
SOURCE  13 ORGANISM_COMMON: BAKER'S YEAST;                                      
SOURCE  14 ORGANISM_TAXID: 4932;                                                
SOURCE  15 GENE: DNL4, LIG4;                                                    
SOURCE  16 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21;                            
SOURCE  17 EXPRESSION_SYSTEM_TAXID: 511693;                                     
SOURCE  18 EXPRESSION_SYSTEM_STRAIN: BL21;                                      
SOURCE  19 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  20 EXPRESSION_SYSTEM_PLASMID: PGAT3;                                    
SOURCE  21 MOL_ID: 3;                                                           
SOURCE  22 ORGANISM_SCIENTIFIC: SACCHAROMYCES CEREVISIAE;                       
SOURCE  23 ORGANISM_COMMON: BAKER'S YEAST;                                      
SOURCE  24 ORGANISM_TAXID: 4932;                                                
SOURCE  25 GENE: LIF1;                                                          
SOURCE  26 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  27 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE  28 MOL_ID: 4;                                                           
SOURCE  29 ORGANISM_SCIENTIFIC: SACCHAROMYCES CEREVISIAE;                       
SOURCE  30 ORGANISM_COMMON: BAKER'S YEAST;                                      
SOURCE  31 ORGANISM_TAXID: 4932;                                                
SOURCE  32 GENE: LIF1;                                                          
SOURCE  33 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  34 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE  35 MOL_ID: 5;                                                           
SOURCE  36 ORGANISM_SCIENTIFIC: SACCHAROMYCES CEREVISIAE;                       
SOURCE  37 ORGANISM_COMMON: BAKER'S YEAST;                                      
SOURCE  38 ORGANISM_TAXID: 4932;                                                
SOURCE  39 GENE: LIF1;                                                          
SOURCE  40 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  41 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE  42 MOL_ID: 6;                                                           
SOURCE  43 ORGANISM_SCIENTIFIC: SACCHAROMYCES CEREVISIAE;                       
SOURCE  44 ORGANISM_COMMON: BAKER'S YEAST;                                      
SOURCE  45 ORGANISM_TAXID: 4932;                                                
SOURCE  46 GENE: LIF1;                                                          
SOURCE  47 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  48 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE  49 MOL_ID: 7;                                                           
SOURCE  50 ORGANISM_SCIENTIFIC: SACCHAROMYCES CEREVISIAE;                       
SOURCE  51 ORGANISM_COMMON: BAKER'S YEAST;                                      
SOURCE  52 ORGANISM_TAXID: 4932;                                                
SOURCE  53 GENE: LIF1;                                                          
SOURCE  54 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  55 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE  56 MOL_ID: 8;                                                           
SOURCE  57 ORGANISM_SCIENTIFIC: SACCHAROMYCES CEREVISIAE;                       
SOURCE  58 ORGANISM_COMMON: BAKER'S YEAST;                                      
SOURCE  59 ORGANISM_TAXID: 4932;                                                
SOURCE  60 GENE: LIF1;                                                          
SOURCE  61 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  62 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE  63 MOL_ID: 9;                                                           
SOURCE  64 ORGANISM_SCIENTIFIC: SACCHAROMYCES CEREVISIAE;                       
SOURCE  65 ORGANISM_COMMON: BAKER'S YEAST;                                      
SOURCE  66 ORGANISM_TAXID: 4932;                                                
SOURCE  67 GENE: LIF1;                                                          
SOURCE  68 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  69 EXPRESSION_SYSTEM_TAXID: 562                                         
KEYWDS    DNA REPAIR, BRCT, NHEJ, XRCC4, DNA LIGASE, COILED-COIL                
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    A.S.DORE,N.FURNHAM,O.R.DAVIES,B.L.SIBANDA,D.Y.CHIRGADZE,              
AUTHOR   2 S.P.JACKSON,L.PELLEGRINI,T.L.BLUNDELL                                
REVDAT   5   24-FEB-09 1Z56    1       VERSN                                    
REVDAT   4   29-AUG-06 1Z56    3       ATOM   REMARK                            
REVDAT   3   08-AUG-06 1Z56    1       JRNL   DBREF                             
REVDAT   2   28-FEB-06 1Z56    1       JRNL                                     
REVDAT   1   31-JAN-06 1Z56    0                                                
JRNL        AUTH   A.S.DORE,N.FURNHAM,O.R.DAVIES,B.L.SIBANDA,                   
JRNL        AUTH 2 D.Y.CHIRGADZE,S.P.JACKSON,L.PELLEGRINI,T.L.BLUNDELL          
JRNL        TITL   STRUCTURE OF AN XRCC4-DNA LIGASE IV YEAST ORTHOLOG           
JRNL        TITL 2 COMPLEX REVEALS A NOVEL BRCT INTERACTION MODE.               
JRNL        REF    DNA REPAIR                    V.   5   362 2006              
JRNL        REFN                   ISSN 1568-7864                               
JRNL        PMID   16388993                                                     
JRNL        DOI    10.1016/J.DNAREP.2005.11.004                                 
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION.    3.92 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : REFMAC 5.2.0005                                      
REMARK   3   AUTHORS     : MURSHUDOV,VAGIN,DODSON                               
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : MAXIMUM LIKELIHOOD                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 3.92                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 49.20                          
REMARK   3   DATA CUTOFF            (SIGMA(F)) : NULL                           
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.8                           
REMARK   3   NUMBER OF REFLECTIONS             : 15638                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   CROSS-VALIDATION METHOD          : THROUGHOUT                      
REMARK   3   FREE R VALUE TEST SET SELECTION  : RANDOM                          
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.403                           
REMARK   3   R VALUE            (WORKING SET) : 0.400                           
REMARK   3   FREE R VALUE                     : 0.467                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.100                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 832                             
REMARK   3                                                                      
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.                                  
REMARK   3   TOTAL NUMBER OF BINS USED           : 20                           
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : 3.92                         
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : 4.02                         
REMARK   3   REFLECTION IN BIN     (WORKING SET) : 1105                         
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : 99.07                        
REMARK   3   BIN R VALUE           (WORKING SET) : 0.3610                       
REMARK   3   BIN FREE R VALUE SET COUNT          : 67                           
REMARK   3   BIN FREE R VALUE                    : 0.4330                       
REMARK   3                                                                      
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.                    
REMARK   3   PROTEIN ATOMS            : 4164                                    
REMARK   3   NUCLEIC ACID ATOMS       : 0                                       
REMARK   3   HETEROGEN ATOMS          : 0                                       
REMARK   3   SOLVENT ATOMS            : 0                                       
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 107.70                         
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : 1.31000                                              
REMARK   3    B22 (A**2) : 1.31000                                              
REMARK   3    B33 (A**2) : -1.96000                                             
REMARK   3    B12 (A**2) : 0.65000                                              
REMARK   3    B13 (A**2) : 0.00000                                              
REMARK   3    B23 (A**2) : 0.00000                                              
REMARK   3                                                                      
REMARK   3  ESTIMATED OVERALL COORDINATE ERROR.                                 
REMARK   3   ESU BASED ON R VALUE                            (A): NULL          
REMARK   3   ESU BASED ON FREE R VALUE                       (A): 0.961         
REMARK   3   ESU BASED ON MAXIMUM LIKELIHOOD                 (A): 1.060         
REMARK   3   ESU FOR B VALUES BASED ON MAXIMUM LIKELIHOOD (A**2): 70.148        
REMARK   3                                                                      
REMARK   3 CORRELATION COEFFICIENTS.                                            
REMARK   3   CORRELATION COEFFICIENT FO-FC      : 0.750                         
REMARK   3   CORRELATION COEFFICIENT FO-FC FREE : 0.659                         
REMARK   3                                                                      
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES        COUNT    RMS    WEIGHT      
REMARK   3   BOND LENGTHS REFINED ATOMS        (A):  4198 ; 0.016 ; 0.021       
REMARK   3   BOND LENGTHS OTHERS               (A):     8 ; 0.012 ; 0.020       
REMARK   3   BOND ANGLES REFINED ATOMS   (DEGREES):  5623 ; 2.130 ; 1.980       
REMARK   3   BOND ANGLES OTHERS          (DEGREES):    16 ; 1.987 ; 3.000       
REMARK   3   TORSION ANGLES, PERIOD 1    (DEGREES):   545 ;10.166 ; 5.000       
REMARK   3   TORSION ANGLES, PERIOD 2    (DEGREES):   158 ;41.914 ;24.241       
REMARK   3   TORSION ANGLES, PERIOD 3    (DEGREES):   809 ;25.971 ;15.000       
REMARK   3   TORSION ANGLES, PERIOD 4    (DEGREES):    31 ;20.912 ;15.000       
REMARK   3   CHIRAL-CENTER RESTRAINTS       (A**3):   656 ; 0.132 ; 0.200       
REMARK   3   GENERAL PLANES REFINED ATOMS      (A):  3049 ; 0.007 ; 0.020       
REMARK   3   GENERAL PLANES OTHERS             (A):     8 ; 0.005 ; 0.020       
REMARK   3   NON-BONDED CONTACTS REFINED ATOMS (A):  2550 ; 0.330 ; 0.200       
REMARK   3   NON-BONDED CONTACTS OTHERS        (A):    18 ; 0.323 ; 0.200       
REMARK   3   NON-BONDED TORSION REFINED ATOMS  (A):  2748 ; 0.324 ; 0.200       
REMARK   3   NON-BONDED TORSION OTHERS         (A):    16 ; 0.254 ; 0.200       
REMARK   3   H-BOND (X...Y) REFINED ATOMS      (A):   246 ; 0.257 ; 0.200       
REMARK   3   H-BOND (X...Y) OTHERS             (A):  NULL ;  NULL ;  NULL       
REMARK   3   POTENTIAL METAL-ION REFINED ATOMS (A):  NULL ;  NULL ;  NULL       
REMARK   3   POTENTIAL METAL-ION OTHERS        (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY VDW REFINED ATOMS        (A):    62 ; 0.325 ; 0.200       
REMARK   3   SYMMETRY VDW OTHERS               (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY H-BOND REFINED ATOMS     (A):     6 ; 0.218 ; 0.200       
REMARK   3   SYMMETRY H-BOND OTHERS            (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY METAL-ION REFINED ATOMS  (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY METAL-ION OTHERS         (A):  NULL ;  NULL ;  NULL       
REMARK   3                                                                      
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.     COUNT   RMS    WEIGHT      
REMARK   3   MAIN-CHAIN BOND REFINED ATOMS  (A**2):  2848 ; 0.596 ; 1.500       
REMARK   3   MAIN-CHAIN BOND OTHER ATOMS    (A**2):     8 ; 0.094 ; 1.500       
REMARK   3   MAIN-CHAIN ANGLE REFINED ATOMS (A**2):  4463 ; 1.042 ; 2.000       
REMARK   3   SIDE-CHAIN BOND REFINED ATOMS  (A**2):  1502 ; 1.203 ; 3.000       
REMARK   3   SIDE-CHAIN ANGLE REFINED ATOMS (A**2):  1160 ; 2.151 ; 4.500       
REMARK   3                                                                      
REMARK   3 ANISOTROPIC THERMAL FACTOR RESTRAINTS.    COUNT   RMS   WEIGHT       
REMARK   3   RIGID-BOND RESTRAINTS          (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   SPHERICITY; FREE ATOMS         (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   SPHERICITY; BONDED ATOMS       (A**2):  NULL ;  NULL ;  NULL       
REMARK   3                                                                      
REMARK   3  NCS RESTRAINTS STATISTICS                                           
REMARK   3   NUMBER OF DIFFERENT NCS GROUPS : NULL                              
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : NULL                                       
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED : MASK                                                 
REMARK   3   PARAMETERS FOR MASK CALCULATION                                    
REMARK   3   VDW PROBE RADIUS   : 1.20                                          
REMARK   3   ION PROBE RADIUS   : 0.80                                          
REMARK   3   SHRINKAGE RADIUS   : 0.80                                          
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: HYDROGENS HAVE BEEN ADDED IN THE          
REMARK   3  RIDING POSITIONS                                                    
REMARK   4                                                                      
REMARK   4 1Z56 COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 07-APR-05.                  
REMARK 100 THE RCSB ID CODE IS RCSB032311.                                      
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 20-AUG-04                          
REMARK 200  TEMPERATURE           (KELVIN) : 100.0                              
REMARK 200  PH                             : 8.0                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : ESRF                               
REMARK 200  BEAMLINE                       : ID13                               
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.968                              
REMARK 200  MONOCHROMATOR                  : SI-111 DOUBLE MONOCHROMATOR        
REMARK 200  OPTICS                         : SI-111 DOUBLE MONOCHROMATOR        
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : MARRESEARCH                        
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : MARXDS                             
REMARK 200  DATA SCALING SOFTWARE          : SCALEPACK                          
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 16475                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 3.920                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 49.200                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : 2.500                              
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.8                               
REMARK 200  DATA REDUNDANCY                : 10.100                             
REMARK 200  R MERGE                    (I) : 0.17500                            
REMARK 200  R SYM                      (I) : 0.17500                            
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 5.7000                             
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 3.92                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 3.99                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 99.6                               
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL                               
REMARK 200  R MERGE FOR SHELL          (I) : 0.79900                            
REMARK 200  R SYM FOR SHELL            (I) : 0.79900                            
REMARK 200  <I/SIGMA(I)> FOR SHELL         : NULL                               
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: SIR(AS)                      
REMARK 200 SOFTWARE USED: SHARP                                                 
REMARK 200 STARTING MODEL: NULL                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 75.10                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 5.00                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: TRIS-HCL, SODIUM CHLORIDE,               
REMARK 280  DITHIOTHREITOL, PEG 6000, 2-(N-MORPHOLINO)ETHANESULFONIC ACID,      
REMARK 280  ETHYLENE GLYCOL, PH 8.0, VAPOR DIFFUSION, HANGING DROP,             
REMARK 280  TEMPERATURE 293.0K                                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 64 2 2                         
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -Y,X-Y,Z+1/3                                            
REMARK 290       3555   -X+Y,-X,Z+2/3                                           
REMARK 290       4555   -X,-Y,Z                                                 
REMARK 290       5555   Y,-X+Y,Z+1/3                                            
REMARK 290       6555   X-Y,X,Z+2/3                                             
REMARK 290       7555   Y,X,-Z+1/3                                              
REMARK 290       8555   X-Y,-Y,-Z                                               
REMARK 290       9555   -X,-X+Y,-Z+2/3                                          
REMARK 290      10555   -Y,-X,-Z+1/3                                            
REMARK 290      11555   -X+Y,Y,-Z                                               
REMARK 290      12555   X,X-Y,-Z+2/3                                            
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -0.500000 -0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.866025 -0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       32.80533            
REMARK 290   SMTRY1   3 -0.500000  0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   3 -0.866025 -0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000       65.61067            
REMARK 290   SMTRY1   4 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   4  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   5  0.500000  0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   5 -0.866025  0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   5  0.000000  0.000000  1.000000       32.80533            
REMARK 290   SMTRY1   6  0.500000 -0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   6  0.866025  0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   6  0.000000  0.000000  1.000000       65.61067            
REMARK 290   SMTRY1   7 -0.500000  0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   7  0.866025  0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   7  0.000000  0.000000 -1.000000       32.80533            
REMARK 290   SMTRY1   8  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   8  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   8  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   9 -0.500000 -0.866025  0.000000        0.00000            
REMARK 290   SMTRY2   9 -0.866025  0.500000  0.000000        0.00000            
REMARK 290   SMTRY3   9  0.000000  0.000000 -1.000000       65.61067            
REMARK 290   SMTRY1  10  0.500000 -0.866025  0.000000        0.00000            
REMARK 290   SMTRY2  10 -0.866025 -0.500000  0.000000        0.00000            
REMARK 290   SMTRY3  10  0.000000  0.000000 -1.000000       32.80533            
REMARK 290   SMTRY1  11 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2  11  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3  11  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1  12  0.500000  0.866025  0.000000        0.00000            
REMARK 290   SMTRY2  12  0.866025 -0.500000  0.000000        0.00000            
REMARK 290   SMTRY3  12  0.000000  0.000000 -1.000000       65.61067            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: UNDECAMERIC                       
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, C, D, E, F, G, H, I,            
REMARK 350                    AND CHAINS: J, K                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     MET A     1                                                      
REMARK 465     SER A     2                                                      
REMARK 465     GLN A     3                                                      
REMARK 465     LEU A     4                                                      
REMARK 465     THR A     5                                                      
REMARK 465     GLU A     6                                                      
REMARK 465     PHE A     7                                                      
REMARK 465     ILE A     8                                                      
REMARK 465     SER A     9                                                      
REMARK 465     CYS A    10                                                      
REMARK 465     ILE A    11                                                      
REMARK 465     PRO A    12                                                      
REMARK 465     VAL A    13                                                      
REMARK 465     VAL A    14                                                      
REMARK 465     ASN A    15                                                      
REMARK 465     GLU A    16                                                      
REMARK 465     GLU A    17                                                      
REMARK 465     GLN A    18                                                      
REMARK 465     ASN A    19                                                      
REMARK 465     GLU A    20                                                      
REMARK 465     GLU A    21                                                      
REMARK 465     ASP A    22                                                      
REMARK 465     GLU A    23                                                      
REMARK 465     ARG A    24                                                      
REMARK 465     GLY A    25                                                      
REMARK 465     LEU A    26                                                      
REMARK 465     CYS A    27                                                      
REMARK 465     LYS A    28                                                      
REMARK 465     ILE A    29                                                      
REMARK 465     GLN A    30                                                      
REMARK 465     ILE A    31                                                      
REMARK 465     GLU A    32                                                      
REMARK 465     ASP A    33                                                      
REMARK 465     GLY A    34                                                      
REMARK 465     ALA A    35                                                      
REMARK 465     MET A    36                                                      
REMARK 465     LEU A    37                                                      
REMARK 465     GLU A    38                                                      
REMARK 465     THR A    39                                                      
REMARK 465     LEU A    40                                                      
REMARK 465     ASP A    41                                                      
REMARK 465     GLU A    42                                                      
REMARK 465     ASN A    43                                                      
REMARK 465     SER A    44                                                      
REMARK 465     LEU A    45                                                      
REMARK 465     SER A    46                                                      
REMARK 465     GLY A    47                                                      
REMARK 465     LEU A    48                                                      
REMARK 465     ARG A    49                                                      
REMARK 465     ILE A    50                                                      
REMARK 465     GLU A    51                                                      
REMARK 465     LYS A    52                                                      
REMARK 465     MET A    53                                                      
REMARK 465     LEU A    54                                                      
REMARK 465     VAL A    55                                                      
REMARK 465     SER A    56                                                      
REMARK 465     GLU A    57                                                      
REMARK 465     GLY A    58                                                      
REMARK 465     THR A    59                                                      
REMARK 465     GLY A    60                                                      
REMARK 465     ILE A    61                                                      
REMARK 465     PHE A    62                                                      
REMARK 465     SER A    63                                                      
REMARK 465     LYS A    64                                                      
REMARK 465     SER A    65                                                      
REMARK 465     SER A    66                                                      
REMARK 465     PHE A    67                                                      
REMARK 465     GLY A    68                                                      
REMARK 465     ILE A    69                                                      
REMARK 465     ASN A    70                                                      
REMARK 465     ASP A    71                                                      
REMARK 465     LEU A    72                                                      
REMARK 465     ARG A    73                                                      
REMARK 465     ILE A    74                                                      
REMARK 465     PHE A    75                                                      
REMARK 465     THR A    76                                                      
REMARK 465     GLY A    77                                                      
REMARK 465     GLU A    78                                                      
REMARK 465     ASN A    79                                                      
REMARK 465     ILE A    80                                                      
REMARK 465     ASP A    81                                                      
REMARK 465     GLU A    82                                                      
REMARK 465     GLU A    83                                                      
REMARK 465     SER A    84                                                      
REMARK 465     LYS A    85                                                      
REMARK 465     LYS A    86                                                      
REMARK 465     TYR A    87                                                      
REMARK 465     VAL A    88                                                      
REMARK 465     TRP A    89                                                      
REMARK 465     TYR A    90                                                      
REMARK 465     GLU A    91                                                      
REMARK 465     LEU A    92                                                      
REMARK 465     LEU A    93                                                      
REMARK 465     LYS A    94                                                      
REMARK 465     MET A    95                                                      
REMARK 465     LEU A    96                                                      
REMARK 465     THR A    97                                                      
REMARK 465     GLY A    98                                                      
REMARK 465     HIS A    99                                                      
REMARK 465     LYS A   100                                                      
REMARK 465     VAL A   101                                                      
REMARK 465     TYR A   102                                                      
REMARK 465     ILE A   103                                                      
REMARK 465     ALA A   104                                                      
REMARK 465     SER A   105                                                      
REMARK 465     LEU A   106                                                      
REMARK 465     ASP A   107                                                      
REMARK 465     GLU A   108                                                      
REMARK 465     LYS A   109                                                      
REMARK 465     VAL A   110                                                      
REMARK 465     VAL A   111                                                      
REMARK 465     PHE A   112                                                      
REMARK 465     THR A   113                                                      
REMARK 465     LYS A   114                                                      
REMARK 465     TRP A   115                                                      
REMARK 465     THR A   116                                                      
REMARK 465     CYS A   117                                                      
REMARK 465     ARG A   118                                                      
REMARK 465     MET A   119                                                      
REMARK 465     GLN A   120                                                      
REMARK 465     ASP A   121                                                      
REMARK 465     ASP A   122                                                      
REMARK 465     GLU A   123                                                      
REMARK 465     VAL A   124                                                      
REMARK 465     TRP A   125                                                      
REMARK 465     LYS A   126                                                      
REMARK 465     VAL A   127                                                      
REMARK 465     VAL A   128                                                      
REMARK 465     MET A   129                                                      
REMARK 465     GLU A   130                                                      
REMARK 465     LEU A   131                                                      
REMARK 465     GLU A   132                                                      
REMARK 465     SER A   133                                                      
REMARK 465     SER A   134                                                      
REMARK 465     ALA A   135                                                      
REMARK 465     ILE A   136                                                      
REMARK 465     ILE A   137                                                      
REMARK 465     ARG A   138                                                      
REMARK 465     LYS A   139                                                      
REMARK 465     ILE A   140                                                      
REMARK 465     ALA A   141                                                      
REMARK 465     GLU A   142                                                      
REMARK 465     LEU A   143                                                      
REMARK 465     THR A   144                                                      
REMARK 465     LEU A   145                                                      
REMARK 465     HIS A   146                                                      
REMARK 465     PRO A   147                                                      
REMARK 465     VAL A   148                                                      
REMARK 465     LYS A   149                                                      
REMARK 465     LYS A   150                                                      
REMARK 465     GLY A   151                                                      
REMARK 465     GLU A   152                                                      
REMARK 465     ILE A   153                                                      
REMARK 465     ASP A   154                                                      
REMARK 465     LEU A   155                                                      
REMARK 465     PHE A   156                                                      
REMARK 465     GLU A   157                                                      
REMARK 465     MET A   158                                                      
REMARK 465     LYS A   236                                                      
REMARK 465     LEU A   237                                                      
REMARK 465     SER A   238                                                      
REMARK 465     ASP A   239                                                      
REMARK 465     ASP A   240                                                      
REMARK 465     ASP A   241                                                      
REMARK 465     VAL A   242                                                      
REMARK 465     LEU A   243                                                      
REMARK 465     ASP A   244                                                      
REMARK 465     SER A   245                                                      
REMARK 465     ALA A   246                                                      
REMARK 465     MET B     1                                                      
REMARK 465     SER B     2                                                      
REMARK 465     GLN B     3                                                      
REMARK 465     LEU B     4                                                      
REMARK 465     THR B     5                                                      
REMARK 465     GLU B     6                                                      
REMARK 465     PHE B     7                                                      
REMARK 465     ILE B     8                                                      
REMARK 465     SER B     9                                                      
REMARK 465     CYS B    10                                                      
REMARK 465     ILE B    11                                                      
REMARK 465     PRO B    12                                                      
REMARK 465     VAL B    13                                                      
REMARK 465     VAL B    14                                                      
REMARK 465     ASN B    15                                                      
REMARK 465     GLU B    16                                                      
REMARK 465     GLU B    17                                                      
REMARK 465     GLN B    18                                                      
REMARK 465     ASN B    19                                                      
REMARK 465     GLU B    20                                                      
REMARK 465     GLU B    21                                                      
REMARK 465     ASP B    22                                                      
REMARK 465     GLU B    23                                                      
REMARK 465     ARG B    24                                                      
REMARK 465     GLY B    25                                                      
REMARK 465     LEU B    26                                                      
REMARK 465     CYS B    27                                                      
REMARK 465     LYS B    28                                                      
REMARK 465     ILE B    29                                                      
REMARK 465     GLN B    30                                                      
REMARK 465     ILE B    31                                                      
REMARK 465     GLU B    32                                                      
REMARK 465     ASP B    33                                                      
REMARK 465     GLY B    34                                                      
REMARK 465     ALA B    35                                                      
REMARK 465     MET B    36                                                      
REMARK 465     LEU B    37                                                      
REMARK 465     GLU B    38                                                      
REMARK 465     THR B    39                                                      
REMARK 465     LEU B    40                                                      
REMARK 465     ASP B    41                                                      
REMARK 465     GLU B    42                                                      
REMARK 465     ASN B    43                                                      
REMARK 465     SER B    44                                                      
REMARK 465     LEU B    45                                                      
REMARK 465     SER B    46                                                      
REMARK 465     GLY B    47                                                      
REMARK 465     LEU B    48                                                      
REMARK 465     ARG B    49                                                      
REMARK 465     ILE B    50                                                      
REMARK 465     GLU B    51                                                      
REMARK 465     LYS B    52                                                      
REMARK 465     MET B    53                                                      
REMARK 465     LEU B    54                                                      
REMARK 465     VAL B    55                                                      
REMARK 465     SER B    56                                                      
REMARK 465     GLU B    57                                                      
REMARK 465     GLY B    58                                                      
REMARK 465     THR B    59                                                      
REMARK 465     GLY B    60                                                      
REMARK 465     ILE B    61                                                      
REMARK 465     PHE B    62                                                      
REMARK 465     SER B    63                                                      
REMARK 465     LYS B    64                                                      
REMARK 465     SER B    65                                                      
REMARK 465     SER B    66                                                      
REMARK 465     PHE B    67                                                      
REMARK 465     GLY B    68                                                      
REMARK 465     ILE B    69                                                      
REMARK 465     ASN B    70                                                      
REMARK 465     ASP B    71                                                      
REMARK 465     LEU B    72                                                      
REMARK 465     ARG B    73                                                      
REMARK 465     ILE B    74                                                      
REMARK 465     PHE B    75                                                      
REMARK 465     THR B    76                                                      
REMARK 465     GLY B    77                                                      
REMARK 465     GLU B    78                                                      
REMARK 465     ASN B    79                                                      
REMARK 465     ILE B    80                                                      
REMARK 465     ASP B    81                                                      
REMARK 465     GLU B    82                                                      
REMARK 465     GLU B    83                                                      
REMARK 465     SER B    84                                                      
REMARK 465     LYS B    85                                                      
REMARK 465     LYS B    86                                                      
REMARK 465     TYR B    87                                                      
REMARK 465     VAL B    88                                                      
REMARK 465     TRP B    89                                                      
REMARK 465     TYR B    90                                                      
REMARK 465     GLU B    91                                                      
REMARK 465     LEU B    92                                                      
REMARK 465     LEU B    93                                                      
REMARK 465     LYS B    94                                                      
REMARK 465     MET B    95                                                      
REMARK 465     LEU B    96                                                      
REMARK 465     THR B    97                                                      
REMARK 465     GLY B    98                                                      
REMARK 465     HIS B    99                                                      
REMARK 465     LYS B   100                                                      
REMARK 465     VAL B   101                                                      
REMARK 465     TYR B   102                                                      
REMARK 465     ILE B   103                                                      
REMARK 465     ALA B   104                                                      
REMARK 465     SER B   105                                                      
REMARK 465     LEU B   106                                                      
REMARK 465     ASP B   107                                                      
REMARK 465     GLU B   108                                                      
REMARK 465     LYS B   109                                                      
REMARK 465     VAL B   110                                                      
REMARK 465     VAL B   111                                                      
REMARK 465     PHE B   112                                                      
REMARK 465     THR B   113                                                      
REMARK 465     LYS B   114                                                      
REMARK 465     TRP B   115                                                      
REMARK 465     THR B   116                                                      
REMARK 465     CYS B   117                                                      
REMARK 465     ARG B   118                                                      
REMARK 465     MET B   119                                                      
REMARK 465     GLN B   120                                                      
REMARK 465     ASP B   121                                                      
REMARK 465     ASP B   122                                                      
REMARK 465     GLU B   123                                                      
REMARK 465     VAL B   124                                                      
REMARK 465     TRP B   125                                                      
REMARK 465     LYS B   126                                                      
REMARK 465     VAL B   127                                                      
REMARK 465     VAL B   128                                                      
REMARK 465     MET B   129                                                      
REMARK 465     GLU B   130                                                      
REMARK 465     LEU B   131                                                      
REMARK 465     GLU B   132                                                      
REMARK 465     SER B   133                                                      
REMARK 465     SER B   134                                                      
REMARK 465     ALA B   135                                                      
REMARK 465     ILE B   136                                                      
REMARK 465     ILE B   137                                                      
REMARK 465     ARG B   138                                                      
REMARK 465     LYS B   139                                                      
REMARK 465     ILE B   140                                                      
REMARK 465     ALA B   141                                                      
REMARK 465     GLU B   142                                                      
REMARK 465     LEU B   143                                                      
REMARK 465     THR B   144                                                      
REMARK 465     LEU B   145                                                      
REMARK 465     HIS B   146                                                      
REMARK 465     PRO B   147                                                      
REMARK 465     VAL B   148                                                      
REMARK 465     LYS B   149                                                      
REMARK 465     LYS B   150                                                      
REMARK 465     GLY B   151                                                      
REMARK 465     GLU B   152                                                      
REMARK 465     ILE B   153                                                      
REMARK 465     ASP B   154                                                      
REMARK 465     LEU B   155                                                      
REMARK 465     GLN B   232                                                      
REMARK 465     ASN B   233                                                      
REMARK 465     ASN B   234                                                      
REMARK 465     ILE B   235                                                      
REMARK 465     LYS B   236                                                      
REMARK 465     LEU B   237                                                      
REMARK 465     SER B   238                                                      
REMARK 465     ASP B   239                                                      
REMARK 465     ASP B   240                                                      
REMARK 465     ASP B   241                                                      
REMARK 465     VAL B   242                                                      
REMARK 465     LEU B   243                                                      
REMARK 465     ASP B   244                                                      
REMARK 465     SER B   245                                                      
REMARK 465     ALA B   246                                                      
REMARK 465     PRO C   681                                                      
REMARK 465     ILE C   682                                                      
REMARK 465     LEU C   839A                                                     
REMARK 465     PHE C   839B                                                     
REMARK 465     SER C   839C                                                     
REMARK 465     ASN C   839D                                                     
REMARK 465     ARG C   839E                                                     
REMARK 465     ILE C   839F                                                     
REMARK 465     ALA C   839G                                                     
REMARK 465     TYR C   839H                                                     
REMARK 465     VAL C   839I                                                     
REMARK 465     PRO C   839J                                                     
REMARK 465     ARG C   839K                                                     
REMARK 465     ARG C   839L                                                     
REMARK 465     LYS C   839M                                                     
REMARK 465     ILE C   839N                                                     
REMARK 465     SER C   839O                                                     
REMARK 465     THR C   839P                                                     
REMARK 465     GLU C   839Q                                                     
REMARK 465     ASP C   839R                                                     
REMARK 465     ASP C   839S                                                     
REMARK 465     ILE C   839T                                                     
REMARK 465     PRO C   939                                                      
REMARK 465     VAL C   940                                                      
REMARK 465     VAL C   941                                                      
REMARK 465     ASN C   942                                                      
REMARK 465     TYR C   943                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS(M=MODEL NUMBER;            
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     PHE C 839    CG   CD1  CD2  CE1  CE2  CZ                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT                     
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.                            
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE          
REMARK 500   ND2  ASN C   895     N    VAL C   897              1.47            
REMARK 500   ND2  ASN C   895     CG2  VAL C   897              1.63            
REMARK 500   ND2  ASN C   895     CA   VAL C   897              1.97            
REMARK 500   O    ASN C   895     N    VAL C   897              1.99            
REMARK 500   ND2  ASN C   895     CB   VAL C   897              2.05            
REMARK 500   O    ASP C   892     CB   ASN C   895              2.05            
REMARK 500   O    ASN C   761     N    ASP C   765              2.09            
REMARK 500   O    PRO C   760     N    TRP C   762              2.09            
REMARK 500   CG   UNK G  4007     O    UNK G  4012              2.15            
REMARK 500   O    PHE C   866     N    GLY C   868              2.16            
REMARK 500   O    CYS C   741     CD2  HIS C   759              2.17            
REMARK 500   O    ILE C   859     N    ILE C   863              2.19            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS                                             
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS THAT ARE RELATED BY CRYSTALLOGRAPHIC             
REMARK 500 SYMMETRY ARE IN CLOSE CONTACT.  AN ATOM LOCATED WITHIN 0.15          
REMARK 500 ANGSTROMS OF A SYMMETRY RELATED ATOM IS ASSUMED TO BE ON A           
REMARK 500 SPECIAL POSITION AND IS, THEREFORE, LISTED IN REMARK 375             
REMARK 500 INSTEAD OF REMARK 500.  ATOMS WITH NON-BLANK ALTERNATE               
REMARK 500 LOCATION INDICATORS ARE NOT INCLUDED IN THE CALCULATIONS.            
REMARK 500                                                                      
REMARK 500 DISTANCE CUTOFF:                                                     
REMARK 500 2.2 ANGSTROMS FOR CONTACTS NOT INVOLVING HYDROGEN ATOMS              
REMARK 500 1.6 ANGSTROMS FOR CONTACTS INVOLVING HYDROGEN ATOMS                  
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI  SSYMOP   DISTANCE          
REMARK 500   NE   ARG C   916     CG2  ILE C   727     6554     1.96            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES                                       
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)              
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3                                     
REMARK 500    LEU B 200   CA  -  CB  -  CG  ANGL. DEV. = -17.3 DEGREES          
REMARK 500    ARG C 835   NE  -  CZ  -  NH1 ANGL. DEV. =   5.5 DEGREES          
REMARK 500    LEU C 876   CA  -  CB  -  CG  ANGL. DEV. =  17.8 DEGREES          
REMARK 500    UNK J3002   O   -  C   -  N   ANGL. DEV. = -12.1 DEGREES          
REMARK 500    UNK J3003   O   -  C   -  N   ANGL. DEV. = -12.0 DEGREES          
REMARK 500    UNK J3005   O   -  C   -  N   ANGL. DEV. = -14.2 DEGREES          
REMARK 500    UNK J3008   O   -  C   -  N   ANGL. DEV. = -36.1 DEGREES          
REMARK 500    UNK J3009   O   -  C   -  N   ANGL. DEV. = -39.6 DEGREES          
REMARK 500    UNK J3010   O   -  C   -  N   ANGL. DEV. = -10.7 DEGREES          
REMARK 500    UNK J3011   O   -  C   -  N   ANGL. DEV. =  14.7 DEGREES          
REMARK 500    UNK J3012   O   -  C   -  N   ANGL. DEV. = -18.4 DEGREES          
REMARK 500    UNK J3016   O   -  C   -  N   ANGL. DEV. = -17.7 DEGREES          
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    CYS A 167      -27.16    -37.20                                   
REMARK 500    VAL A 169       10.55    -62.65                                   
REMARK 500    TYR A 173       44.70    -79.65                                   
REMARK 500    ARG A 174      -48.95   -153.08                                   
REMARK 500    LYS A 177       39.45    -99.50                                   
REMARK 500    GLU A 178      -77.91   -165.12                                   
REMARK 500    ASP A 180      -14.16   -144.12                                   
REMARK 500    SER A 181     -101.68    -82.94                                   
REMARK 500    GLU A 188      -40.72   -143.60                                   
REMARK 500    LEU A 190       47.63   -103.04                                   
REMARK 500    ALA A 191      -39.70   -152.54                                   
REMARK 500    GLU A 195        1.14    -69.46                                   
REMARK 500    LEU A 201        8.86    -61.48                                   
REMARK 500    MET A 211      -79.99    -67.65                                   
REMARK 500    MET A 212      -59.17    -26.36                                   
REMARK 500    ASN A 234      150.94    -47.96                                   
REMARK 500    GLU B 157       30.64    -85.46                                   
REMARK 500    TYR B 163       24.87    -66.01                                   
REMARK 500    ILE B 166      -22.00    -23.89                                   
REMARK 500    CYS B 167     -116.18    -75.69                                   
REMARK 500    ARG B 174       -6.17    -50.43                                   
REMARK 500    LYS B 177      -83.30    -37.81                                   
REMARK 500    GLU B 178        4.87    -63.42                                   
REMARK 500    SER B 181      -73.78    -87.75                                   
REMARK 500    GLU B 188      -84.25    -50.56                                   
REMARK 500    ALA B 191      -62.10    -97.15                                   
REMARK 500    ARG B 192      -33.74    -37.72                                   
REMARK 500    GLU B 193      -84.64    -58.81                                   
REMARK 500    GLU B 195        0.34    -54.18                                   
REMARK 500    LEU B 196       13.24   -141.00                                   
REMARK 500    LEU B 197      -88.80   -118.53                                   
REMARK 500    ASP B 198      -67.99    -20.38                                   
REMARK 500    MET B 212      -75.24    -50.35                                   
REMARK 500    VAL B 213      -76.68    -36.77                                   
REMARK 500    LYS B 222       27.06    -72.26                                   
REMARK 500    LEU B 226      -79.01    -37.10                                   
REMARK 500    GLU B 228       55.77   -109.18                                   
REMARK 500    ASN C 684       88.89     57.57                                   
REMARK 500    PHE C 686      -94.84     47.34                                   
REMARK 500    LEU C 690       74.12    -68.65                                   
REMARK 500    VAL C 693        9.44   -174.19                                   
REMARK 500    GLU C 700      161.88     69.72                                   
REMARK 500    THR C 702     -157.20   -142.89                                   
REMARK 500    ILE C 704      -76.83    -82.45                                   
REMARK 500    THR C 707     -152.95     51.11                                   
REMARK 500    GLU C 710       73.91   -118.59                                   
REMARK 500    LEU C 711      -70.53     25.08                                   
REMARK 500    HIS C 718      -40.93   -148.48                                   
REMARK 500    TYR C 724      148.73    167.83                                   
REMARK 500    LEU C 728      115.43    -18.26                                   
REMARK 500    HIS C 731      119.11    -14.30                                   
REMARK 500    SER C 732       -0.76   -145.42                                   
REMARK 500    ILE C 733       21.50    -77.51                                   
REMARK 500    ASP C 735       79.44   -109.12                                   
REMARK 500    VAL C 736       57.74   -108.65                                   
REMARK 500    THR C 744      -81.90   -102.77                                   
REMARK 500    THR C 745     -120.38     17.11                                   
REMARK 500    GLU C 746      -36.06    -28.04                                   
REMARK 500    LYS C 748      -74.80    -11.49                                   
REMARK 500    ALA C 749        4.34    -38.06                                   
REMARK 500    ASP C 752       30.50    -82.54                                   
REMARK 500    ARG C 753       32.00   -154.32                                   
REMARK 500    HIS C 759      163.38    171.47                                   
REMARK 500    PRO C 760      -98.81    -84.03                                   
REMARK 500    ASN C 761      -65.54     49.94                                   
REMARK 500    TYR C 769     -117.75     77.02                                   
REMARK 500    LEU C 772       33.18   -162.31                                   
REMARK 500    ILE C 773       92.64    -53.53                                   
REMARK 500    LEU C 774       42.93    -54.60                                   
REMARK 500    PRO C 777      101.66    -32.94                                   
REMARK 500    ASN C 778       79.34   -178.01                                   
REMARK 500    CYS C 780       10.25   -141.59                                   
REMARK 500    ASN C 782      107.64     64.51                                   
REMARK 500    SER C 784      -86.01    -40.89                                   
REMARK 500    MET C 787      -46.28   -144.08                                   
REMARK 500    ASP C 796     -166.29   -112.17                                   
REMARK 500    SER C 801     -173.90    -58.70                                   
REMARK 500    GLU C 803     -116.12    -52.13                                   
REMARK 500    ASP C 805      -99.45     20.66                                   
REMARK 500    SER C 813      -82.13    -56.22                                   
REMARK 500    TYR C 815      -54.53   -172.35                                   
REMARK 500    LYS C 816       25.14    -73.96                                   
REMARK 500    LEU C 819      -50.35   -161.74                                   
REMARK 500    LEU C 821      103.43   -164.86                                   
REMARK 500    PRO C 823     -151.75    -48.14                                   
REMARK 500    MET C 824     -164.72    -72.40                                   
REMARK 500    ASP C 830     -120.20   -107.55                                   
REMARK 500    SER C 831     -126.30   -170.30                                   
REMARK 500    LYS C 869      105.11     64.69                                   
REMARK 500    ILE C 870     -126.81   -162.77                                   
REMARK 500    THR C 871      -70.84    156.43                                   
REMARK 500    ASP C 872     -122.24     85.11                                   
REMARK 500    GLN C 873      112.27     95.43                                   
REMARK 500    GLN C 874       38.21    -89.92                                   
REMARK 500    LEU C 876       93.40    -14.75                                   
REMARK 500    ASN C 878      -47.56     21.11                                   
REMARK 500    TYR C 884      173.23    -46.93                                   
REMARK 500    THR C 885      -68.47    121.34                                   
REMARK 500    ASP C 886     -137.25   -120.31                                   
REMARK 500    MET C 894        2.19    -61.68                                   
REMARK 500    ASN C 895      140.43    -25.19                                   
REMARK 500    GLU C 896      -27.73     13.00                                   
REMARK 500    LYS C 900       10.46    -63.26                                   
REMARK 500    LYS C 902       48.43    -84.27                                   
REMARK 500    ALA C 907     -113.60    -76.79                                   
REMARK 500    SER C 908      131.32    -24.10                                   
REMARK 500    ALA C 919      141.45    -35.61                                   
REMARK 500    GLU C 921      -42.99    -14.63                                   
REMARK 500    TRP C 922      -73.21    -59.28                                   
REMARK 500    VAL C 923      -57.94    -26.94                                   
REMARK 500    HIS C 925        3.74    -62.74                                   
REMARK 500    ILE C 927      -21.65    -36.60                                   
REMARK 500    GLU C 929      -69.09   -174.63                                   
REMARK 500    ASN C 930     -138.64   -161.40                                   
REMARK 500    GLN C 932     -166.13   -115.43                                   
REMARK 500    VAL C 933      -44.40   -160.25                                   
REMARK 500    GLU C 935       81.81    -58.76                                   
REMARK 500    GLU C 936       18.91    177.64                                   
REMARK 500    ASP C 937     -138.04    -70.50                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: NON-CIS, NON-TRANS                                         
REMARK 500                                                                      
REMARK 500 THE FOLLOWING PEPTIDE BONDS DEVIATE SIGNIFICANTLY FROM BOTH          
REMARK 500 CIS AND TRANS CONFORMATION.  CIS BONDS, IF ANY, ARE LISTED           
REMARK 500 ON CISPEP RECORDS.  TRANS IS DEFINED AS 180 +/- 30 AND               
REMARK 500 CIS IS DEFINED AS 0 +/- 30 DEGREES.                                  
REMARK 500                                 MODEL     OMEGA                      
REMARK 500 TYR C  724     ASN C  725                 -146.46                    
REMARK 500 ARG C  771     LEU C  772                  148.17                    
REMARK 500 ASP C  805     ILE C  806                 -133.57                    
REMARK 500 TYR C  884     THR C  885                  141.30                    
REMARK 500 ASN C  895     GLU C  896                  118.46                    
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: MAIN CHAIN PLANARITY                                       
REMARK 500                                                                      
REMARK 500 THE FOLLOWING RESIDUES HAVE A PSEUDO PLANARITY                       
REMARK 500 TORSION, C(I) - CA(I) - N(I+1) - O(I), GREATER                       
REMARK 500 10.0 DEGREES. (M=MODEL NUMBER; RES=RESIDUE NAME;                     
REMARK 500 C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;                            
REMARK 500 I=INSERTION CODE).                                                   
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        ANGLE                                           
REMARK 500    UNK J3001         63.77                                           
REMARK 500    UNK J3002        106.68                                           
REMARK 500    UNK J3003        137.60                                           
REMARK 500    UNK J3004        111.41                                           
REMARK 500    UNK J3005        144.53                                           
REMARK 500    UNK J3006        111.88                                           
REMARK 500    UNK J3007       -136.15                                           
REMARK 500    UNK J3008        150.97                                           
REMARK 500    UNK J3009         92.75                                           
REMARK 500    UNK J3010       -142.00                                           
REMARK 500    UNK J3011         72.36                                           
REMARK 500    UNK J3012        159.80                                           
REMARK 500    UNK J3013        172.45                                           
REMARK 500    UNK J3014        150.43                                           
REMARK 500    UNK J3015        158.64                                           
REMARK 500    UNK J3016        100.12                                           
REMARK 500    UNK J3017         64.75                                           
REMARK 500    UNK J3018        134.92                                           
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 650                                                                      
REMARK 650  HELIX                                                               
REMARK 650 DETERMINATION METHOD: AUTHOR DEFINED                                 
REMARK 700                                                                      
REMARK 700  SHEET                                                               
REMARK 700 DETERMINATION METHOD: AUTHOR DEFINED                                 
REMARK 999                                                                      
REMARK 999  SEQUENCE                                                            
REMARK 999 AS STATED BY THE AUTHORS, THE RESIDUES IN CHAINS D,E,                
REMARK 999 F,G,H,I,J,K COULD NOT BE ALIGNED WITH ANY REGION OF                  
REMARK 999 THE TWO CHAINS A AND B DUE TO LOW ELECTRON DENSITY                   
REMARK 999 AND THEREFORE HAVE BEEN LABELED UNK.                                 
DBREF  1Z56 A    1   246  UNP    P53150   LIF1_YEAST       1    246             
DBREF  1Z56 B    1   246  UNP    P53150   LIF1_YEAST       1    246             
DBREF  1Z56 C  681   942  UNP    Q08387   DNL4_YEAST     681    944             
DBREF  1Z56 D 1000  1007  PDB    1Z56     1Z56          1000   1007             
DBREF  1Z56 E 2000  2006  PDB    1Z56     1Z56          2000   2006             
DBREF  1Z56 F 3000  3044  PDB    1Z56     1Z56          3000   3044             
DBREF  1Z56 G 4000  4036  PDB    1Z56     1Z56          4000   4036             
DBREF  1Z56 H 1000  1006  PDB    1Z56     1Z56          1000   1006             
DBREF  1Z56 I 2000  2029  PDB    1Z56     1Z56          2000   2029             
DBREF  1Z56 J 3000  3019  PDB    1Z56     1Z56          3000   3019             
DBREF  1Z56 K 4000  4012  PDB    1Z56     1Z56          4000   4012             
SEQRES   1 A  246  MET SER GLN LEU THR GLU PHE ILE SER CYS ILE PRO VAL          
SEQRES   2 A  246  VAL ASN GLU GLU GLN ASN GLU GLU ASP GLU ARG GLY LEU          
SEQRES   3 A  246  CYS LYS ILE GLN ILE GLU ASP GLY ALA MET LEU GLU THR          
SEQRES   4 A  246  LEU ASP GLU ASN SER LEU SER GLY LEU ARG ILE GLU LYS          
SEQRES   5 A  246  MET LEU VAL SER GLU GLY THR GLY ILE PHE SER LYS SER          
SEQRES   6 A  246  SER PHE GLY ILE ASN ASP LEU ARG ILE PHE THR GLY GLU          
SEQRES   7 A  246  ASN ILE ASP GLU GLU SER LYS LYS TYR VAL TRP TYR GLU          
SEQRES   8 A  246  LEU LEU LYS MET LEU THR GLY HIS LYS VAL TYR ILE ALA          
SEQRES   9 A  246  SER LEU ASP GLU LYS VAL VAL PHE THR LYS TRP THR CYS          
SEQRES  10 A  246  ARG MET GLN ASP ASP GLU VAL TRP LYS VAL VAL MET GLU          
SEQRES  11 A  246  LEU GLU SER SER ALA ILE ILE ARG LYS ILE ALA GLU LEU          
SEQRES  12 A  246  THR LEU HIS PRO VAL LYS LYS GLY GLU ILE ASP LEU PHE          
SEQRES  13 A  246  GLU MET ALA ASP LYS LEU TYR LYS ASP ILE CYS CYS VAL          
SEQRES  14 A  246  ASN ASP SER TYR ARG ASN ILE LYS GLU SER ASP SER SER          
SEQRES  15 A  246  ASN ARG ASN ARG VAL GLU GLN LEU ALA ARG GLU ARG GLU          
SEQRES  16 A  246  LEU LEU ASP LYS LEU LEU GLU THR ARG ASP GLU ARG THR          
SEQRES  17 A  246  ARG ALA MET MET VAL THR LEU LEU ASN GLU LYS LYS LYS          
SEQRES  18 A  246  LYS ILE ARG GLU LEU HIS GLU ILE LEU ARG GLN ASN ASN          
SEQRES  19 A  246  ILE LYS LEU SER ASP ASP ASP VAL LEU ASP SER ALA              
SEQRES   1 B  246  MET SER GLN LEU THR GLU PHE ILE SER CYS ILE PRO VAL          
SEQRES   2 B  246  VAL ASN GLU GLU GLN ASN GLU GLU ASP GLU ARG GLY LEU          
SEQRES   3 B  246  CYS LYS ILE GLN ILE GLU ASP GLY ALA MET LEU GLU THR          
SEQRES   4 B  246  LEU ASP GLU ASN SER LEU SER GLY LEU ARG ILE GLU LYS          
SEQRES   5 B  246  MET LEU VAL SER GLU GLY THR GLY ILE PHE SER LYS SER          
SEQRES   6 B  246  SER PHE GLY ILE ASN ASP LEU ARG ILE PHE THR GLY GLU          
SEQRES   7 B  246  ASN ILE ASP GLU GLU SER LYS LYS TYR VAL TRP TYR GLU          
SEQRES   8 B  246  LEU LEU LYS MET LEU THR GLY HIS LYS VAL TYR ILE ALA          
SEQRES   9 B  246  SER LEU ASP GLU LYS VAL VAL PHE THR LYS TRP THR CYS          
SEQRES  10 B  246  ARG MET GLN ASP ASP GLU VAL TRP LYS VAL VAL MET GLU          
SEQRES  11 B  246  LEU GLU SER SER ALA ILE ILE ARG LYS ILE ALA GLU LEU          
SEQRES  12 B  246  THR LEU HIS PRO VAL LYS LYS GLY GLU ILE ASP LEU PHE          
SEQRES  13 B  246  GLU MET ALA ASP LYS LEU TYR LYS ASP ILE CYS CYS VAL          
SEQRES  14 B  246  ASN ASP SER TYR ARG ASN ILE LYS GLU SER ASP SER SER          
SEQRES  15 B  246  ASN ARG ASN ARG VAL GLU GLN LEU ALA ARG GLU ARG GLU          
SEQRES  16 B  246  LEU LEU ASP LYS LEU LEU GLU THR ARG ASP GLU ARG THR          
SEQRES  17 B  246  ARG ALA MET MET VAL THR LEU LEU ASN GLU LYS LYS LYS          
SEQRES  18 B  246  LYS ILE ARG GLU LEU HIS GLU ILE LEU ARG GLN ASN ASN          
SEQRES  19 B  246  ILE LYS LEU SER ASP ASP ASP VAL LEU ASP SER ALA              
SEQRES   1 C  264  PRO ILE SER ASN ILE PHE ALA GLY LEU LEU PHE TYR VAL          
SEQRES   2 C  264  LEU SER ASP TYR VAL THR GLU ASP THR GLY ILE ARG ILE          
SEQRES   3 C  264  THR ARG ALA GLU LEU GLU LYS THR ILE VAL GLU HIS GLY          
SEQRES   4 C  264  GLY LYS LEU ILE TYR ASN VAL ILE LEU LYS ARG HIS SER          
SEQRES   5 C  264  ILE GLY ASP VAL ARG LEU ILE SER CYS LYS THR THR THR          
SEQRES   6 C  264  GLU CYS LYS ALA LEU ILE ASP ARG GLY TYR ASP ILE LEU          
SEQRES   7 C  264  HIS PRO ASN TRP VAL LEU ASP CYS ILE ALA TYR LYS ARG          
SEQRES   8 C  264  LEU ILE LEU ILE GLU PRO ASN TYR CYS PHE ASN VAL SER          
SEQRES   9 C  264  GLN LYS MET ARG ALA VAL ALA GLU LYS ARG VAL ASP CYS          
SEQRES  10 C  264  LEU GLY ASP SER PHE GLU ASN ASP ILE SER GLU THR LYS          
SEQRES  11 C  264  LEU SER SER LEU TYR LYS SER GLN LEU SER LEU PRO PRO          
SEQRES  12 C  264  MET GLY GLU LEU GLU ILE ASP SER GLU VAL ARG ARG PHE          
SEQRES  13 C  264  PRO LEU PHE LEU PHE SER ASN ARG ILE ALA TYR VAL PRO          
SEQRES  14 C  264  ARG ARG LYS ILE SER THR GLU ASP ASP ILE ILE GLU MET          
SEQRES  15 C  264  LYS ILE LYS LEU PHE GLY GLY LYS ILE THR ASP GLN GLN          
SEQRES  16 C  264  SER LEU CYS ASN LEU ILE ILE ILE PRO TYR THR ASP PRO          
SEQRES  17 C  264  ILE LEU ARG LYS ASP CYS MET ASN GLU VAL HIS GLU LYS          
SEQRES  18 C  264  ILE LYS GLU GLN ILE LYS ALA SER ASP THR ILE PRO LYS          
SEQRES  19 C  264  ILE ALA ARG VAL VAL ALA PRO GLU TRP VAL ASP HIS SER          
SEQRES  20 C  264  ILE ASN GLU ASN CYS GLN VAL PRO GLU GLU ASP PHE PRO          
SEQRES  21 C  264  VAL VAL ASN TYR                                              
SEQRES   1 D    8  UNK UNK UNK UNK UNK UNK UNK UNK                              
SEQRES   1 E    7  UNK UNK UNK UNK UNK UNK UNK                                  
SEQRES   1 F   45  UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK          
SEQRES   2 F   45  UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK          
SEQRES   3 F   45  UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK          
SEQRES   4 F   45  UNK UNK UNK UNK UNK UNK                                      
SEQRES   1 G   37  UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK          
SEQRES   2 G   37  UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK          
SEQRES   3 G   37  UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK                  
SEQRES   1 H    7  UNK UNK UNK UNK UNK UNK UNK                                  
SEQRES   1 I   30  UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK          
SEQRES   2 I   30  UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK          
SEQRES   3 I   30  UNK UNK UNK UNK                                              
SEQRES   1 J   20  UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK          
SEQRES   2 J   20  UNK UNK UNK UNK UNK UNK UNK                                  
SEQRES   1 K   13  UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK UNK          
HELIX    1   1 ALA A  159  ARG A  231  1                                  73    
HELIX    2   2 PHE B  156  ILE B  229  1                                  74    
HELIX    3   3 GLU C  710  GLY C  720  1                                  11    
HELIX    4   4 GLU C  746  ILE C  751  1                                   6    
HELIX    5   5 ASN C  761  ALA C  768  1                                   8    
HELIX    6   6 LYS C  786  ASP C  796  1                                  11    
HELIX    7   7 SER C  807  LYS C  816  1                                  10    
HELIX    8   8 LYS C  862  GLY C  868  1                                   7    
HELIX    9   9 LEU C  889  SER C  908  1                                  20    
HELIX   10  10 PRO C  920  ILE C  927  1                                   8    
SHEET    1   A 4 SER C 695  THR C 699  0                                        
SHEET    2   A 4 TYR C 724  VAL C 726  1  O  ASN C 725   N  SER C 695           
SHEET    3   A 4 ARG C 737  CYS C 741  1  O  LEU C 738   N  TYR C 724           
SHEET    4   A 4 ASP C 756  HIS C 759  1  O  ILE C 757   N  ARG C 737           
SHEET    1   B 6 VAL C 795  CYS C 797  0                                        
SHEET    2   B 6 GLY C 799  SER C 801  1  O  ASP C 800   N  VAL C 795           
SHEET    1   C10 PRO C 837  PHE C 839  0                                        
SHEET    2   C10 THR C 871  GLN C 874  1  O  ASP C 872   N  PRO C 837           
SHEET    3   C10 ASN C 878  ILE C 881  1  O  LEU C 879   N  THR C 871           
SHEET    4   C10 ALA C 915  VAL C 918  1  O  ARG C 916   N  ASN C 878           
CISPEP   1 HIS C  759    PRO C  760          0       -27.11                     
CRYST1  247.617  247.617   98.416  90.00  90.00 120.00 P 64 2 2     24          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.004038  0.002332  0.000000        0.00000                         
SCALE2      0.000000  0.004663  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.010161        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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