GenomeNet

Database: PDB
Entry: 1ZC8
LinkDB: 1ZC8
Original site: 1ZC8 
HEADER    RNA BINDING PROTEIN/RNA                 11-APR-05   1ZC8              
TITLE     COORDINATES OF TMRNA, SMPB, EF-TU AND H44 FITTED INTO CRYO-EM MAP OF  
TITLE    2 THE 70S RIBOSOME AND TMRNA COMPLEX                                   
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: TLD 16S RIBOSOMAL RNA;                                     
COMPND   3 CHAIN: A;                                                            
COMPND   4 MOL_ID: 2;                                                           
COMPND   5 MOLECULE: H2 16S RRNA;                                               
COMPND   6 CHAIN: B;                                                            
COMPND   7 MOL_ID: 3;                                                           
COMPND   8 MOLECULE: H2B D MRNA;                                                
COMPND   9 CHAIN: C;                                                            
COMPND  10 MOL_ID: 4;                                                           
COMPND  11 MOLECULE: PROTEIN KINASE 4 MRNA;                                     
COMPND  12 CHAIN: G;                                                            
COMPND  13 OTHER_DETAILS: PK1;                                                  
COMPND  14 MOL_ID: 5;                                                           
COMPND  15 MOLECULE: H5 16S RIBOSOMAL RNA;                                      
COMPND  16 CHAIN: F;                                                            
COMPND  17 MOL_ID: 6;                                                           
COMPND  18 MOLECULE: PROTEIN KINASE 2 MRNA;                                     
COMPND  19 CHAIN: H;                                                            
COMPND  20 OTHER_DETAILS: PK2;                                                  
COMPND  21 MOL_ID: 7;                                                           
COMPND  22 MOLECULE: PROTEIN KINASE 3;                                          
COMPND  23 CHAIN: I;                                                            
COMPND  24 OTHER_DETAILS: PK3;                                                  
COMPND  25 MOL_ID: 8;                                                           
COMPND  26 MOLECULE: PROTEIN KINASE 4 MRNA;                                     
COMPND  27 CHAIN: J;                                                            
COMPND  28 OTHER_DETAILS: PK4;                                                  
COMPND  29 MOL_ID: 9;                                                           
COMPND  30 MOLECULE: H44 16S RIBOSOMAL RNA;                                     
COMPND  31 CHAIN: Z;                                                            
COMPND  32 MOL_ID: 10;                                                          
COMPND  33 MOLECULE: SSRA-BINDING PROTEIN;                                      
COMPND  34 CHAIN: K;                                                            
COMPND  35 MOL_ID: 11;                                                          
COMPND  36 MOLECULE: ELONGATION FACTOR TU;                                      
COMPND  37 CHAIN: Y;                                                            
COMPND  38 SYNONYM: EF-TU                                                       
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: THERMUS THERMOPHILUS;                           
SOURCE   3 ORGANISM_TAXID: 274;                                                 
SOURCE   4 MOL_ID: 2;                                                           
SOURCE   5 ORGANISM_SCIENTIFIC: THERMUS THERMOPHILUS;                           
SOURCE   6 ORGANISM_TAXID: 274;                                                 
SOURCE   7 MOL_ID: 3;                                                           
SOURCE   8 ORGANISM_SCIENTIFIC: THERMUS THERMOPHILUS;                           
SOURCE   9 ORGANISM_TAXID: 274;                                                 
SOURCE  10 MOL_ID: 4;                                                           
SOURCE  11 ORGANISM_SCIENTIFIC: THERMUS THERMOPHILUS;                           
SOURCE  12 ORGANISM_TAXID: 274;                                                 
SOURCE  13 MOL_ID: 5;                                                           
SOURCE  14 ORGANISM_SCIENTIFIC: THERMUS THERMOPHILUS;                           
SOURCE  15 ORGANISM_TAXID: 274;                                                 
SOURCE  16 MOL_ID: 6;                                                           
SOURCE  17 ORGANISM_SCIENTIFIC: THERMUS THERMOPHILUS;                           
SOURCE  18 ORGANISM_TAXID: 274;                                                 
SOURCE  19 MOL_ID: 7;                                                           
SOURCE  20 ORGANISM_SCIENTIFIC: THERMUS THERMOPHILUS;                           
SOURCE  21 ORGANISM_TAXID: 274;                                                 
SOURCE  22 MOL_ID: 8;                                                           
SOURCE  23 ORGANISM_SCIENTIFIC: THERMUS THERMOPHILUS;                           
SOURCE  24 ORGANISM_TAXID: 274;                                                 
SOURCE  25 MOL_ID: 9;                                                           
SOURCE  26 ORGANISM_SCIENTIFIC: THERMUS THERMOPHILUS;                           
SOURCE  27 ORGANISM_TAXID: 274;                                                 
SOURCE  28 MOL_ID: 10;                                                          
SOURCE  29 ORGANISM_SCIENTIFIC: THERMUS THERMOPHILUS;                           
SOURCE  30 ORGANISM_TAXID: 274;                                                 
SOURCE  31 MOL_ID: 11;                                                          
SOURCE  32 ORGANISM_SCIENTIFIC: THERMUS THERMOPHILUS;                           
SOURCE  33 ORGANISM_TAXID: 274                                                  
KEYWDS    SMPB, TMRNA, EF-TU, H44, 30S, 16S RRNA, CRYO-EM, TRANS-TRANSLATION,   
KEYWDS   2 70S, RNA BINDING PROTEIN-RNA COMPLEX                                 
EXPDTA    ELECTRON MICROSCOPY                                                   
MDLTYP    CA ATOMS ONLY, CHAIN K, Y; P ATOMS ONLY, CHAIN A, B, C, G, F, H, I, J,
MDLTYP   2 Z                                                                    
AUTHOR    M.VALLE,R.GILLET,S.KAUR,A.HENNE,V.RAMAKRISHNAN,J.FRANK                
REVDAT   3   11-DEC-13 1ZC8    1       ATOM   VERSN                             
REVDAT   2   24-FEB-09 1ZC8    1       VERSN                                    
REVDAT   1   19-APR-05 1ZC8    0                                                
JRNL        AUTH   M.VALLE,R.GILLET,S.KAUR,A.HENNE,V.RAMAKRISHNAN,J.FRANK       
JRNL        TITL   VISUALIZING TMRNA ENTRY INTO A STALLED RIBOSOME              
JRNL        REF    SCIENCE                       V. 300   127 2003              
JRNL        REFN                   ISSN 0036-8075                               
JRNL        PMID   12677067                                                     
JRNL        DOI    10.1126/SCIENCE.1081798                                      
REMARK   1                                                                      
REMARK   1 REFERENCE 1                                                          
REMARK   1  AUTH   G.DONG,J.NOWAKOWSKI,D.W.HOFFMAN                              
REMARK   1  TITL   STRUCTURE OF SMALL PROTEIN B: THE PROTEIN COMPONENT OF THE   
REMARK   1  TITL 2 TMRNA SMPB SYSTEM FOR RIBOSOME RESCUE                        
REMARK   1  REF    EMBO J.                       V.  21  1845 2002              
REMARK   1  REFN                   ISSN 0261-4189                               
REMARK   1 REFERENCE 2                                                          
REMARK   1  AUTH   B.KNUDSEN,J.WOWER,C.ZWIEB,J.GORODKIN                         
REMARK   1  TITL   TMRDB (TMRNA DATABASE)                                       
REMARK   1  REF    NUCLEIC ACIDS RES.            V.  29   171 2001              
REMARK   1  REFN                   ISSN 0305-1048                               
REMARK   1 REFERENCE 3                                                          
REMARK   1  AUTH   M.VALLE,J.SENGUPTA,N.K.SWAMI,R.A.GRASSUCCI,N.BURKHARDT,      
REMARK   1  AUTH 2 K.H.NIERHAUS,R.K.AGRAWAL,J.FRANK                             
REMARK   1  TITL   CRYO-EM REVEALS AN ACTIVE ROLE FOR AMINOACYL-TRNA IN THE     
REMARK   1  TITL 2 ACCOMMODATION PROCESS                                        
REMARK   1  REF    EMBO J.                       V.  21  3557 2002              
REMARK   1  REFN                   ISSN 0261-4189                               
REMARK   1 REFERENCE 4                                                          
REMARK   1  AUTH   P.NISSEN,M.KJELDGAARD,S.THIRUP,J.NYBORG                      
REMARK   1  TITL   E. COLI CYSTEINYL-TRNA AND T. AQUATICUS ELONGATION FACTOR    
REMARK   1  TITL 2 EF-TU: GTP TERNARY COMPLEX                                   
REMARK   1  REF    STRUCTURE FOLD.DES.           V.   7   143 1999              
REMARK   1  REFN                   ISSN 0969-2126                               
REMARK   1 REFERENCE 5                                                          
REMARK   1  AUTH   J.M.OGLE,F.V.MURPHY IV,M.J.TARRY,V.RAMAKRISHNAN              
REMARK   1  TITL   SELECTION OF TRNA BY THE RIBOSOME REQUIRES A TRANSITION FROM 
REMARK   1  TITL 2 AN OPEN TO A CLOSED FORM                                     
REMARK   1  REF    CELL(CAMBRIDGE,MASS.)         V. 111   721 2002              
REMARK   1  REFN                   ISSN 0092-8674                               
REMARK   2                                                                      
REMARK   2 RESOLUTION.   13.00 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   SOFTWARE PACKAGES      : NULL                                      
REMARK   3   RECONSTRUCTION SCHEMA  : 3D PROJECTION MATCHING: CONJUGATE         
REMARK   3                            GRADIENTS WITH REGULARIZATION             
REMARK   3                                                                      
REMARK   3 EM MAP-MODEL FITTING AND REFINEMENT                                  
REMARK   3   PDB ENTRY                    : 1K8H, 1B23, 1N32                    
REMARK   3   REFINEMENT SPACE             : REAL                                
REMARK   3   REFINEMENT PROTOCOL          : NULL                                
REMARK   3   REFINEMENT TARGET            : NULL                                
REMARK   3   OVERALL ANISOTROPIC B VALUE  : NULL                                
REMARK   3                                                                      
REMARK   3 FITTING PROCEDURE : MANUAL FITTING IN O                              
REMARK   3                                                                      
REMARK   3 EM IMAGE RECONSTRUCTION STATISTICS                                   
REMARK   3   NOMINAL PIXEL SIZE (ANGSTROMS)    : NULL                           
REMARK   3   ACTUAL PIXEL SIZE  (ANGSTROMS)    : 2.820                          
REMARK   3   EFFECTIVE RESOLUTION (ANGSTROMS)  : 13.000                         
REMARK   3   NUMBER OF PARTICLES               : NULL                           
REMARK   3   CTF CORRECTION METHOD             : CTF CORRECTION OF 3D-MAPS BY   
REMARK   3                                       WIENER FILTRATION              
REMARK   3                                                                      
REMARK   3 EM RECONSTRUCTION MAGNIFICATION CALIBRATION: TMV                     
REMARK   3                                                                      
REMARK   3 OTHER DETAILS: SPIDER PACKAGE, THIS ENTRY CONTAINS ONLY C ALPHA      
REMARK   3  AND P ATOMS.                                                        
REMARK   4                                                                      
REMARK   4 1ZC8 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 12-APR-05.                  
REMARK 100 THE RCSB ID CODE IS RCSB032557.                                      
REMARK 245                                                                      
REMARK 245 EXPERIMENTAL DETAILS                                                 
REMARK 245   RECONSTRUCTION METHOD          : SINGLE PARTICLE                   
REMARK 245   SPECIMEN TYPE                  : VITREOUS ICE (CRYO EM)            
REMARK 245                                                                      
REMARK 245 ELECTRON MICROSCOPE SAMPLE                                           
REMARK 245   SAMPLE TYPE                    : PARTICLE                          
REMARK 245   PARTICLE TYPE                  : ASYMMETRIC                        
REMARK 245   NAME OF SAMPLE                 : NULL                              
REMARK 245   SAMPLE CONCENTRATION (MG ML-1) : 0.03                              
REMARK 245   SAMPLE SUPPORT DETAILS         : QUANTIFOIL HOLLEY-CARBON FILM     
REMARK 245                                    GRIDS                             
REMARK 245   SAMPLE VITRIFICATION DETAILS   : RAPID-FREEZING IN LIQUID ETHANE   
REMARK 245   SAMPLE BUFFER                  : NULL                              
REMARK 245   PH                             : 7.50                              
REMARK 245   SAMPLE DETAILS                 : NULL                              
REMARK 245                                                                      
REMARK 245 DATA ACQUISITION                                                     
REMARK 245   DATE OF EXPERIMENT                : 01-AUG-03                      
REMARK 245   NUMBER OF MICROGRAPHS-IMAGES      : NULL                           
REMARK 245   TEMPERATURE (KELVIN)              : 93.00                          
REMARK 245   MICROSCOPE MODEL                  : FEI TECNAI F20                 
REMARK 245   DETECTOR TYPE                     : KODAK SO163 FILM               
REMARK 245   MINIMUM DEFOCUS (NM)              : 3700.00                        
REMARK 245   MAXIMUM DEFOCUS (NM)              : 1450.00                        
REMARK 245   MINIMUM TILT ANGLE (DEGREES)      : 0.00                           
REMARK 245   MAXIMUM TILT ANGLE (DEGREES)      : 0.00                           
REMARK 245   NOMINAL CS                        : 2.00                           
REMARK 245   IMAGING MODE                      : BRIGHT FIELD                   
REMARK 245   ELECTRON DOSE (ELECTRONS NM**-2)  : 1500.00                        
REMARK 245   ILLUMINATION MODE                 : FLOOD BEAM                     
REMARK 245   NOMINAL MAGNIFICATION             : 49000                          
REMARK 245   CALIBRATED MAGNIFICATION          : 52000                          
REMARK 245   SOURCE                            : FIELD EMISSION GUN             
REMARK 245   ACCELERATION VOLTAGE (KV)         : 200                            
REMARK 245   IMAGING DETAILS                   : NULL                           
REMARK 247                                                                      
REMARK 247 ELECTRON MICROSCOPY                                                  
REMARK 247  THE COORDINATES IN THIS ENTRY WERE GENERATED FROM ELECTRON          
REMARK 247  MICROSCOPY DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE              
REMARK 247  THAT CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES           
REMARK 247  ON THESE RECORDS ARE MEANINGLESS EXCEPT FOR THE CALCULATION         
REMARK 247  OF THE STRUCTURE FACTORS.                                           
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 1                              
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: UNDECAMERIC                       
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, C, G, F, H, I, J, Z,            
REMARK 350                    AND CHAINS: K, Y                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT                     
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.                            
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE          
REMARK 500   P      C I    15     P      U I    44              0.57            
REMARK 500   P      C G    18     P      C J    19              0.79            
REMARK 500   P      C A   348     CA   TYR K    19              2.10            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 1K8H   RELATED DB: PDB                                   
REMARK 900 STRUCTURE OF SMPB                                                    
REMARK 900 RELATED ID: 1B23   RELATED DB: PDB                                   
REMARK 900 STRUCTURE OF EF-TU                                                   
REMARK 900 RELATED ID: 1N32   RELATED DB: PDB                                   
REMARK 900 STRUCTURE OF H44                                                     
REMARK 900 RELATED ID: EMD-1122   RELATED DB: EMDB                              
REMARK 999                                                                      
REMARK 999 SEQUENCE AUTHOR STATES THAT THE MAP WAS FOR THERMUS THERMOPHILUS,    
REMARK 999 BUT THE EF-TU MODEL WAS FROM THERMUS AQUATICUS (SWS CODE Q01698).    
DBREF  1ZC8 A    1   359  PDB    1ZC8     1ZC8             1    359             
DBREF  1ZC8 B    1    16  PDB    1ZC8     1ZC8             1     16             
DBREF  1ZC8 C    1    31  PDB    1ZC8     1ZC8             1     31             
DBREF  1ZC8 G    1    30  PDB    1ZC8     1ZC8             1     30             
DBREF  1ZC8 F    1    29  PDB    1ZC8     1ZC8             1     29             
DBREF  1ZC8 H    1    59  PDB    1ZC8     1ZC8             1     59             
DBREF  1ZC8 I    1    49  PDB    1ZC8     1ZC8             1     49             
DBREF  1ZC8 J    1    52  PDB    1ZC8     1ZC8             1     52             
DBREF  1ZC8 Z 1406  1496  PDB    1ZC8     1ZC8          1406   1496             
DBREF  1ZC8 K    1   130  PDB    1ZC8     1ZC8             1    130             
DBREF  1ZC8 Y    1   405  PDB    1ZC8     1ZC8             1    405             
SEQRES   1 A   59    G   G   G   G   C   U   G   A   U   U   C   U   G          
SEQRES   2 A   59    G   A   U   U   C   G   A   C   G   G   G   A   U          
SEQRES   3 A   59    A   U   U   U   C   G   G   A   C   G   C   G   G          
SEQRES   4 A   59    G   U   U   C   A   A   C   U   C   C   C   G   C          
SEQRES   5 A   59    C   A   G   C   U   C   C                                  
SEQRES   1 B   16    U   U   G   C   G   A   A   A   C   A   U   G   U          
SEQRES   2 B   16    A   G   G                                                  
SEQRES   1 C   31    C   C   C   A   A   G   G   U   G   C   A   U   G          
SEQRES   2 C   31    C   G   C   A   U   G   U   A   G   U   A   C   C          
SEQRES   3 C   31    G   A   G   G   A                                          
SEQRES   1 G   30    C   G   A   G   G   G   G   C   G   G   U   U   G          
SEQRES   2 G   30    G   C   C   U   C   G   U   A   A   A   A   A   G          
SEQRES   3 G   30    C   C   G   C                                              
SEQRES   1 F   29    C   U   U   U   A   G   C   A   G   C   U   U   A          
SEQRES   2 F   29    A   U   A   A   C   C   U   G   C   U   U   A   G          
SEQRES   3 F   29    A   G   C                                                  
SEQRES   1 H   59    C   C   U   C   U   C   U   C   C   C   U   A   G          
SEQRES   2 H   59    C   C   U   C   C   G   C   U   C   U   U   A   G          
SEQRES   3 H   59    G   A   C   G   G   G   G   A   U   C   A   A   G          
SEQRES   4 H   59    A   G   A   G   G   U   C   A   A   A   C   C   C          
SEQRES   5 H   59    A   A   A   A   G   A   G                                  
SEQRES   1 I   49    A   U   C   G   C   G   U   G   G   A   A   G   C          
SEQRES   2 I   49    C   C   U   G   C   C   U   G   G   G   G   U   U          
SEQRES   3 I   49    G   A   A   G   C   G   U   U   A   A   A   A   C          
SEQRES   4 I   49    U   U   A   A   U   C   A   G   G   C                      
SEQRES   1 J   52    G   U   U   U   G   U   U   A   G   U   G   G   C          
SEQRES   2 J   52    G   U   G   U   C   C   G   U   C   C   G   C   A          
SEQRES   3 J   52    G   C   U   G   G   C   A   A   G   C   G   A   A          
SEQRES   4 J   52    U   G   U   A   A   A   G   A   C   U   G   A   C          
SEQRES   1 Z   91    G   C   C   C   G   U   C   A   C   G   C   C   A          
SEQRES   2 Z   91    U   G   G   G   A   G   C   G   G   G   C   U   C          
SEQRES   3 Z   91    U   A   C   C   C   G   A   A   G   U   C   G   C          
SEQRES   4 Z   91    C   G   G   G   A   G   C   C   U   A   C   G   G          
SEQRES   5 Z   91    G   C   A   G   G   C   G   C   C   G   A   G   G          
SEQRES   6 Z   91    G   U   A   G   G   G   C   C   C   G   U   G   A          
SEQRES   7 Z   91    C   U   G   G   G   G   C   G   A   A   G   U   C          
SEQRES   1 K  130  GLY LYS SER ASP LYS ILE ILE PRO ILE ALA GLU ASN LYS          
SEQRES   2 K  130  GLU ALA LYS ALA LYS TYR ASP ILE LEU GLU THR TYR GLU          
SEQRES   3 K  130  ALA GLY ILE VAL LEU LYS GLY SER GLU VAL LYS SER LEU          
SEQRES   4 K  130  ARG GLU LYS GLY THR VAL SER PHE LYS ASP SER PHE VAL          
SEQRES   5 K  130  ARG ILE GLU ASN GLY GLU ALA TRP LEU TYR ASN LEU TYR          
SEQRES   6 K  130  ILE ALA PRO TYR LYS HIS ALA THR ILE GLU ASN HIS ASP          
SEQRES   7 K  130  PRO LEU ARG LYS ARG LYS LEU LEU LEU HIS LYS ARG GLU          
SEQRES   8 K  130  ILE MET ARG LEU TYR GLY LYS VAL GLN GLU LYS GLY TYR          
SEQRES   9 K  130  THR ILE ILE PRO LEU LYS LEU TYR TRP LYS ASN ASN LYS          
SEQRES  10 K  130  VAL LYS VAL LEU ILE ALA LEU ALA LYS GLY LYS LYS LEU          
SEQRES   1 Y  405  ALA LYS GLY GLU PHE ILE ARG THR LYS PRO HIS VAL ASN          
SEQRES   2 Y  405  VAL GLY THR ILE GLY HIS VAL ASP HIS GLY LYS THR THR          
SEQRES   3 Y  405  LEU THR ALA ALA LEU THR TYR VAL ALA ALA ALA GLU ASN          
SEQRES   4 Y  405  PRO ASN VAL GLU VAL LYS ASP TYR GLY ASP ILE ASP LYS          
SEQRES   5 Y  405  ALA PRO GLU GLU ARG ALA ARG GLY ILE THR ILE ASN THR          
SEQRES   6 Y  405  ALA HIS VAL GLU TYR GLU THR ALA LYS ARG HIS TYR SER          
SEQRES   7 Y  405  HIS VAL ASP CYS PRO GLY HIS ALA ASP TYR ILE LYS ASN          
SEQRES   8 Y  405  MET ILE THR GLY ALA ALA GLN MET ASP GLY ALA ILE LEU          
SEQRES   9 Y  405  VAL VAL SER ALA ALA ASP GLY PRO MET PRO GLN THR ARG          
SEQRES  10 Y  405  GLU HIS ILE LEU LEU ALA ARG GLN VAL GLY VAL PRO TYR          
SEQRES  11 Y  405  ILE VAL VAL PHE MET ASN LYS VAL ASP MET VAL ASP ASP          
SEQRES  12 Y  405  PRO GLU LEU LEU ASP LEU VAL GLU MET GLU VAL ARG ASP          
SEQRES  13 Y  405  LEU LEU ASN GLN TYR GLU PHE PRO GLY ASP GLU VAL PRO          
SEQRES  14 Y  405  VAL ILE ARG GLY SER ALA LEU LEU ALA LEU GLU GLU MET          
SEQRES  15 Y  405  HIS LYS ASN PRO LYS THR LYS ARG GLY GLU ASN GLU TRP          
SEQRES  16 Y  405  VAL ASP LYS ILE TRP GLU LEU LEU ASP ALA ILE ASP GLU          
SEQRES  17 Y  405  TYR ILE PRO THR PRO VAL ARG ASP VAL ASP LYS PRO PHE          
SEQRES  18 Y  405  LEU MET PRO VAL GLU ASP VAL PHE THR ILE THR GLY ARG          
SEQRES  19 Y  405  GLY THR VAL ALA THR GLY ARG ILE GLU ARG GLY LYS VAL          
SEQRES  20 Y  405  LYS VAL GLY ASP GLU VAL GLU ILE VAL GLY LEU ALA PRO          
SEQRES  21 Y  405  GLU THR ARG LYS THR VAL VAL THR GLY VAL GLU MET HIS          
SEQRES  22 Y  405  ARG LYS THR LEU GLN GLU GLY ILE ALA GLY ASP ASN VAL          
SEQRES  23 Y  405  GLY LEU LEU LEU ARG GLY VAL SER ARG GLU GLU VAL GLU          
SEQRES  24 Y  405  ARG GLY GLN VAL LEU ALA LYS PRO GLY SER ILE THR PRO          
SEQRES  25 Y  405  HIS THR LYS PHE GLU ALA SER VAL TYR ILE LEU LYS LYS          
SEQRES  26 Y  405  GLU GLU GLY GLY ARG HIS THR GLY PHE PHE THR GLY TYR          
SEQRES  27 Y  405  ARG PRO GLN PHE TYR PHE ARG THR THR ASP VAL THR GLY          
SEQRES  28 Y  405  VAL VAL ARG LEU PRO GLN GLY VAL GLU MET VAL MET PRO          
SEQRES  29 Y  405  GLY ASP ASN VAL THR PHE THR VAL GLU LEU ILE LYS PRO          
SEQRES  30 Y  405  VAL ALA LEU GLU GLU GLY LEU ARG PHE ALA ILE ARG GLU          
SEQRES  31 Y  405  GLY GLY ARG THR VAL GLY ALA GLY VAL VAL THR LYS ILE          
SEQRES  32 Y  405  LEU GLU                                                      
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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