HEADER TRANSCRIPTION 19-MAY-05 2CQC
TITLE SOLUTION STRUCTURE OF THE RNA RECOGNITION MOTIF IN ARGININE/SERINE-
TITLE 2 RICH SPLICING FACTOR 10
COMPND MOL_ID: 1;
COMPND 2 MOLECULE: ARGININE/SERINE-RICH SPLICING FACTOR 10;
COMPND 3 CHAIN: A;
COMPND 4 FRAGMENT: RNA RECOGNITION MOTIF;
COMPND 5 SYNONYM: TRANSFORMER-2-BETA, HTRA2- BETA, TRANSFORMER 2 PROTEIN
COMPND 6 HOMOLOG;
COMPND 7 ENGINEERED: YES
SOURCE MOL_ID: 1;
SOURCE 2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;
SOURCE 3 ORGANISM_COMMON: HUMAN;
SOURCE 4 ORGANISM_TAXID: 9606;
SOURCE 5 GENE: SFRS10;
SOURCE 6 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;
SOURCE 7 EXPRESSION_SYSTEM_PLASMID: P040517-05;
SOURCE 8 OTHER_DETAILS: CELL-FREE PROTEIN SYNTHESIS
KEYWDS RNA RECOGNITION MOTIF, STRUCTURAL GENOMICS, NPPSFA, NATIONAL PROJECT
KEYWDS 2 ON PROTEIN STRUCTURAL AND FUNCTIONAL ANALYSES, RIKEN STRUCTURAL
KEYWDS 3 GENOMICS/PROTEOMICS INITIATIVE, RSGI, TRANSCRIPTION
EXPDTA SOLUTION NMR
NUMMDL 20
AUTHOR T.SOMEYA,Y.MUTO,M.INOUE,T.KIGAWA,T.TERADA,M.SHIROUZU,S.YOKOYAMA,RIKEN
AUTHOR 2 STRUCTURAL GENOMICS/PROTEOMICS INITIATIVE (RSGI)
REVDAT 3 09-MAR-22 2CQC 1 REMARK SEQADV
REVDAT 2 24-FEB-09 2CQC 1 VERSN
REVDAT 1 19-NOV-05 2CQC 0
JRNL AUTH T.SOMEYA,Y.MUTO,M.INOUE,T.KIGAWA,T.TERADA,M.SHIROUZU,
JRNL AUTH 2 S.YOKOYAMA
JRNL TITL SOLUTION STRUCTURE OF THE RNA RECOGNITION MOTIF IN
JRNL TITL 2 ARGININE/SERINE-RICH SPLICING FACTOR 10
JRNL REF TO BE PUBLISHED
JRNL REFN
REMARK 2
REMARK 2 RESOLUTION. NOT APPLICABLE.
REMARK 3
REMARK 3 REFINEMENT.
REMARK 3 PROGRAM : XWINNMR 3.5, CYANA 2.0.17
REMARK 3 AUTHORS : BRUKER (XWINNMR), GUNTERT, P. (CYANA)
REMARK 3
REMARK 3 OTHER REFINEMENT REMARKS: NULL
REMARK 4
REMARK 4 2CQC COMPLIES WITH FORMAT V. 3.15, 01-DEC-08
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 24-MAY-05.
REMARK 100 THE DEPOSITION ID IS D_1000024503.
REMARK 210
REMARK 210 EXPERIMENTAL DETAILS
REMARK 210 EXPERIMENT TYPE : NMR
REMARK 210 TEMPERATURE (KELVIN) : 298
REMARK 210 PH : 7.0
REMARK 210 IONIC STRENGTH : 120MM
REMARK 210 PRESSURE : AMBIENT
REMARK 210 SAMPLE CONTENTS : 1.14MM PROTEIN U-15N,13C; 20MM D
REMARK 210 -TRIS-HCL(PH7.0); 100MM NACL;
REMARK 210 1MM D-DTT; 0.02% NAN3; 10% D2O
REMARK 210
REMARK 210 NMR EXPERIMENTS CONDUCTED : 3D_15N-SEPARATED_NOESY; 3D_13C
REMARK 210 -SEPARATED_NOESY
REMARK 210 SPECTROMETER FIELD STRENGTH : 800 MHZ
REMARK 210 SPECTROMETER MODEL : AVANCE
REMARK 210 SPECTROMETER MANUFACTURER : BRUKER
REMARK 210
REMARK 210 STRUCTURE DETERMINATION.
REMARK 210 SOFTWARE USED : NMRPIPE 20030801, NMRVIEW 5.0.4,
REMARK 210 KUJIRA 0.9295, CYANA 2.0.17
REMARK 210 METHOD USED : TORSION ANGLE DYNAMICS,
REMARK 210 RESTRAINTED MOLECULAR DYNAMICS
REMARK 210
REMARK 210 CONFORMERS, NUMBER CALCULATED : 100
REMARK 210 CONFORMERS, NUMBER SUBMITTED : 20
REMARK 210 CONFORMERS, SELECTION CRITERIA : TARGET FUNCTION, STRUCTURES WITH
REMARK 210 THE LOWEST ENERGY, STRUCTURES
REMARK 210 WITH THE LEAST RESTRAINT
REMARK 210 VIOLATIONS
REMARK 210
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1
REMARK 210
REMARK 210 REMARK: NULL
REMARK 215
REMARK 215 NMR STUDY
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION
REMARK 215 NMR DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE THAT
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON
REMARK 215 THESE RECORDS ARE MEANINGLESS.
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW. BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000
REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000
REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500 M RES CSSEQI PSI PHI
REMARK 500 1 ALA A 112 -54.28 -124.34
REMARK 500 1 PRO A 114 94.36 -69.79
REMARK 500 1 LEU A 127 -70.77 -41.92
REMARK 500 1 GLN A 154 -71.49 -90.90
REMARK 500 1 ARG A 156 41.71 -87.08
REMARK 500 1 PRO A 194 -166.03 -69.79
REMARK 500 2 SER A 107 39.60 34.39
REMARK 500 2 ASP A 115 72.86 -107.19
REMARK 500 2 PHE A 161 145.56 -171.04
REMARK 500 2 GLU A 167 -73.14 -51.52
REMARK 500 2 LYS A 176 -36.20 -35.30
REMARK 500 2 ARG A 178 31.19 -91.44
REMARK 500 2 ALA A 179 -47.71 -132.32
REMARK 500 2 PRO A 194 -175.06 -69.74
REMARK 500 2 SER A 196 40.22 36.77
REMARK 500 3 ASN A 110 76.17 -113.99
REMARK 500 3 ASN A 117 110.88 -175.09
REMARK 500 3 SER A 126 154.15 -44.92
REMARK 500 3 ALA A 145 -65.22 -93.95
REMARK 500 3 GLN A 154 -62.42 -103.50
REMARK 500 3 SER A 155 177.76 -59.69
REMARK 500 3 PRO A 194 84.99 -69.78
REMARK 500 4 ASN A 110 41.08 -100.30
REMARK 500 4 PRO A 116 2.38 -69.76
REMARK 500 4 LYS A 176 -33.69 -34.38
REMARK 500 4 ARG A 178 34.34 -94.82
REMARK 500 4 ALA A 179 -57.20 -133.29
REMARK 500 4 ASN A 180 129.63 -37.08
REMARK 500 4 PRO A 194 0.39 -69.77
REMARK 500 5 ALA A 112 42.74 -97.08
REMARK 500 5 PRO A 116 2.52 -69.68
REMARK 500 5 SER A 126 150.24 -47.82
REMARK 500 5 LYS A 140 -36.54 -39.06
REMARK 500 5 LYS A 176 -30.17 -36.99
REMARK 500 5 ASN A 180 121.49 -34.89
REMARK 500 5 PRO A 194 87.50 -69.81
REMARK 500 6 ASP A 115 98.96 -36.75
REMARK 500 6 PRO A 116 0.09 -69.76
REMARK 500 6 CYS A 118 43.02 -108.16
REMARK 500 6 GLN A 153 -63.98 -91.85
REMARK 500 6 SER A 158 136.49 -34.56
REMARK 500 6 GLU A 167 -72.22 -37.49
REMARK 500 6 LYS A 176 -30.11 -38.91
REMARK 500 6 ARG A 178 32.35 -87.54
REMARK 500 6 ALA A 179 -54.79 -130.18
REMARK 500 7 CYS A 118 43.57 -83.94
REMARK 500 7 ARG A 156 49.30 -94.33
REMARK 500 7 SER A 158 141.44 -35.38
REMARK 500 7 LYS A 176 -34.36 -36.74
REMARK 500 7 ALA A 179 -57.59 -125.21
REMARK 500
REMARK 500 THIS ENTRY HAS 151 RAMACHANDRAN OUTLIERS.
REMARK 500
REMARK 500 REMARK: NULL
REMARK 650
REMARK 650 HELIX
REMARK 650 DETERMINATION METHOD: AUTHOR DETERMINED
REMARK 700
REMARK 700 SHEET
REMARK 700 DETERMINATION METHOD: AUTHOR DETERMINED
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: HSS001003317 RELATED DB: TARGETDB
DBREF 2CQC A 110 191 UNP P62995 TRA2B_HUMAN 110 191
SEQADV 2CQC GLY A 103 UNP P62995 CLONING ARTIFACT
SEQADV 2CQC SER A 104 UNP P62995 CLONING ARTIFACT
SEQADV 2CQC SER A 105 UNP P62995 CLONING ARTIFACT
SEQADV 2CQC GLY A 106 UNP P62995 CLONING ARTIFACT
SEQADV 2CQC SER A 107 UNP P62995 CLONING ARTIFACT
SEQADV 2CQC SER A 108 UNP P62995 CLONING ARTIFACT
SEQADV 2CQC GLY A 109 UNP P62995 CLONING ARTIFACT
SEQADV 2CQC SER A 192 UNP P62995 CLONING ARTIFACT
SEQADV 2CQC GLY A 193 UNP P62995 CLONING ARTIFACT
SEQADV 2CQC PRO A 194 UNP P62995 CLONING ARTIFACT
SEQADV 2CQC SER A 195 UNP P62995 CLONING ARTIFACT
SEQADV 2CQC SER A 196 UNP P62995 CLONING ARTIFACT
SEQADV 2CQC GLY A 197 UNP P62995 CLONING ARTIFACT
SEQRES 1 A 95 GLY SER SER GLY SER SER GLY ASN ARG ALA ASN PRO ASP
SEQRES 2 A 95 PRO ASN CYS CYS LEU GLY VAL PHE GLY LEU SER LEU TYR
SEQRES 3 A 95 THR THR GLU ARG ASP LEU ARG GLU VAL PHE SER LYS TYR
SEQRES 4 A 95 GLY PRO ILE ALA ASP VAL SER ILE VAL TYR ASP GLN GLN
SEQRES 5 A 95 SER ARG ARG SER ARG GLY PHE ALA PHE VAL TYR PHE GLU
SEQRES 6 A 95 ASN VAL ASP ASP ALA LYS GLU ALA LYS GLU ARG ALA ASN
SEQRES 7 A 95 GLY MET GLU LEU ASP GLY ARG ARG ILE ARG VAL SER GLY
SEQRES 8 A 95 PRO SER SER GLY
HELIX 1 1 GLU A 131 PHE A 138 1 8
HELIX 2 2 VAL A 169 ARG A 178 1 10
SHEET 1 A 5 LEU A 120 PHE A 123 0
SHEET 2 A 5 VAL A 147 ILE A 149 -1
SHEET 3 A 5 PHE A 161 VAL A 164 -1
SHEET 4 A 5 MET A 182 LEU A 184 -1
SHEET 5 A 5 ARG A 187 SER A 192 -1
CRYST1 1.000 1.000 1.000 90.00 90.00 90.00 P 1 1
ORIGX1 1.000000 0.000000 0.000000 0.00000
ORIGX2 0.000000 1.000000 0.000000 0.00000
ORIGX3 0.000000 0.000000 1.000000 0.00000
SCALE1 1.000000 0.000000 0.000000 0.00000
SCALE2 0.000000 1.000000 0.000000 0.00000
SCALE3 0.000000 0.000000 1.000000 0.00000
MODEL 1
(ATOM LINES ARE NOT SHOWN.)
END
