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Entry: 2I6L
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HEADER    TRANSFERASE                             29-AUG-06   2I6L              
TITLE     CRYSTAL STRUCTURE OF HUMAN MITOGEN ACTIVATED PROTEIN KINASE           
TITLE    2 6 (MAPK6)                                                            
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: MITOGEN-ACTIVATED PROTEIN KINASE 6;                        
COMPND   3 CHAIN: A, B;                                                         
COMPND   4 SYNONYM: EXTRACELLULAR SIGNAL-REGULATED KINASE 3, ERK-3,             
COMPND   5 MAP KINASE ISOFORM P97, P97- MAPK;                                   
COMPND   6 EC: 2.7.11.24;                                                       
COMPND   7 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: MAPK6, ERK3, PRKM6;                                            
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE   8 EXPRESSION_SYSTEM_STRAIN: BL21 (DE3)-R3;                             
SOURCE   9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  10 EXPRESSION_SYSTEM_PLASMID: PNIC28-BSA4                               
KEYWDS    MAPK6, ERK3, MITOGEN ACTIVATED PROTEIN KINASE 6,                      
KEYWDS   2 EXTRACELLULAR SIGNAL REGULATED KINASE 3, SERINE                      
KEYWDS   3 PHOSPHORYLATION, THREONINE PHOSPHORYLATION, STRUCTURAL               
KEYWDS   4 GENOMICS, STRUCTURAL GENOMICS CONSORTIUM, SGC, TRANSFERASE           
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    P.FILIPPAKOPOULOS,A.P.TURNBULL,P.SAVITSKY,O.FEDOROV,                  
AUTHOR   2 G.BERRIDGE,S.DAS,J.ELKINS,M.SOUNDARARAJAN,X.W.YANG,D.DOYLE,          
AUTHOR   3 E.PAPAGRIGORIOU,A.C.W.PIKE,G.BUNKONCZI,E.UGOCHUKWU,                  
AUTHOR   4 J.DEBRECZENI,F.VON DELFT,A.EDWARDS,C.ARROWSMITH,M.SUNDSTROM,         
AUTHOR   5 S.KNAPP,STRUCTURAL GENOMICS CONSORTIUM (SGC)                         
REVDAT   2   24-FEB-09 2I6L    1       VERSN                                    
REVDAT   1   12-SEP-06 2I6L    0                                                
JRNL        AUTH   P.FILIPPAKOPOULOS,A.P.TURNBULL,P.SAVITSKY,                   
JRNL        AUTH 2 O.FEDOROV,G.BERRIDGE,S.DAS,J.ELKINS,                         
JRNL        AUTH 3 M.SOUNDARARAJAN,X.W.YANG,D.DOYLE,E.PAPAGRIGORIOU,            
JRNL        AUTH 4 A.C.W.PIKE,G.BUNKONCZI,E.UGOCHUKWU,J.DEBRECZENI,             
JRNL        AUTH 5 F.VON DELFT,A.EDWARDS,C.ARROWSMITH,M.SUNDSTROM,              
JRNL        AUTH 6 S.KNAPP                                                      
JRNL        TITL   CRYSTAL STRUCTURE OF THE HUMAN MITOGEN ACTIVATED             
JRNL        TITL 2 PROTEIN KINASE 6                                             
JRNL        REF    TO BE PUBLISHED                                              
JRNL        REFN                                                                
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION.    2.25 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : REFMAC 5.2.0019                                      
REMARK   3   AUTHORS     : MURSHUDOV,VAGIN,DODSON                               
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : MAXIMUM LIKELIHOOD                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.25                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 49.30                          
REMARK   3   DATA CUTOFF            (SIGMA(F)) : 0.000                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.7                           
REMARK   3   NUMBER OF REFLECTIONS             : 30965                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   CROSS-VALIDATION METHOD          : THROUGHOUT                      
REMARK   3   FREE R VALUE TEST SET SELECTION  : RANDOM                          
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.224                           
REMARK   3   R VALUE            (WORKING SET) : 0.222                           
REMARK   3   FREE R VALUE                     : 0.272                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.000                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 1561                            
REMARK   3                                                                      
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.                                  
REMARK   3   TOTAL NUMBER OF BINS USED           : 20                           
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : 2.25                         
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : 2.31                         
REMARK   3   REFLECTION IN BIN     (WORKING SET) : 2076                         
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : 98.34                        
REMARK   3   BIN R VALUE           (WORKING SET) : 0.2160                       
REMARK   3   BIN FREE R VALUE SET COUNT          : 121                          
REMARK   3   BIN FREE R VALUE                    : 0.3030                       
REMARK   3                                                                      
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.                    
REMARK   3   PROTEIN ATOMS            : 4085                                    
REMARK   3   NUCLEIC ACID ATOMS       : 0                                       
REMARK   3   HETEROGEN ATOMS          : 0                                       
REMARK   3   SOLVENT ATOMS            : 79                                      
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 30.74                          
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : 1.14000                                              
REMARK   3    B22 (A**2) : 1.14000                                              
REMARK   3    B33 (A**2) : -2.29000                                             
REMARK   3    B12 (A**2) : 0.00000                                              
REMARK   3    B13 (A**2) : 0.00000                                              
REMARK   3    B23 (A**2) : 0.00000                                              
REMARK   3                                                                      
REMARK   3  ESTIMATED OVERALL COORDINATE ERROR.                                 
REMARK   3   ESU BASED ON R VALUE                            (A): 0.290         
REMARK   3   ESU BASED ON FREE R VALUE                       (A): 0.234         
REMARK   3   ESU BASED ON MAXIMUM LIKELIHOOD                 (A): 0.167         
REMARK   3   ESU FOR B VALUES BASED ON MAXIMUM LIKELIHOOD (A**2): 13.030        
REMARK   3                                                                      
REMARK   3 CORRELATION COEFFICIENTS.                                            
REMARK   3   CORRELATION COEFFICIENT FO-FC      : 0.938                         
REMARK   3   CORRELATION COEFFICIENT FO-FC FREE : 0.905                         
REMARK   3                                                                      
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES        COUNT    RMS    WEIGHT      
REMARK   3   BOND LENGTHS REFINED ATOMS        (A):  4161 ; 0.008 ; 0.022       
REMARK   3   BOND LENGTHS OTHERS               (A):  2624 ; 0.001 ; 0.020       
REMARK   3   BOND ANGLES REFINED ATOMS   (DEGREES):  5673 ; 1.114 ; 1.981       
REMARK   3   BOND ANGLES OTHERS          (DEGREES):  6494 ; 0.996 ; 3.000       
REMARK   3   TORSION ANGLES, PERIOD 1    (DEGREES):   552 ; 5.516 ; 5.000       
REMARK   3   TORSION ANGLES, PERIOD 2    (DEGREES):   131 ;35.114 ;24.733       
REMARK   3   TORSION ANGLES, PERIOD 3    (DEGREES):   641 ;12.665 ;15.000       
REMARK   3   TORSION ANGLES, PERIOD 4    (DEGREES):     9 ;26.312 ;15.000       
REMARK   3   CHIRAL-CENTER RESTRAINTS       (A**3):   701 ; 0.090 ; 0.200       
REMARK   3   GENERAL PLANES REFINED ATOMS      (A):  4596 ; 0.003 ; 0.020       
REMARK   3   GENERAL PLANES OTHERS             (A):   760 ; 0.001 ; 0.020       
REMARK   3   NON-BONDED CONTACTS REFINED ATOMS (A):   824 ; 0.195 ; 0.200       
REMARK   3   NON-BONDED CONTACTS OTHERS        (A):  2593 ; 0.178 ; 0.200       
REMARK   3   NON-BONDED TORSION REFINED ATOMS  (A):  2120 ; 0.169 ; 0.200       
REMARK   3   NON-BONDED TORSION OTHERS         (A):  2039 ; 0.086 ; 0.200       
REMARK   3   H-BOND (X...Y) REFINED ATOMS      (A):   137 ; 0.137 ; 0.200       
REMARK   3   H-BOND (X...Y) OTHERS             (A):     1 ; 0.009 ; 0.200       
REMARK   3   POTENTIAL METAL-ION REFINED ATOMS (A):  NULL ;  NULL ;  NULL       
REMARK   3   POTENTIAL METAL-ION OTHERS        (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY VDW REFINED ATOMS        (A):     4 ; 0.105 ; 0.200       
REMARK   3   SYMMETRY VDW OTHERS               (A):    41 ; 0.164 ; 0.200       
REMARK   3   SYMMETRY H-BOND REFINED ATOMS     (A):     4 ; 0.134 ; 0.200       
REMARK   3   SYMMETRY H-BOND OTHERS            (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY METAL-ION REFINED ATOMS  (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY METAL-ION OTHERS         (A):  NULL ;  NULL ;  NULL       
REMARK   3                                                                      
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.     COUNT   RMS    WEIGHT      
REMARK   3   MAIN-CHAIN BOND REFINED ATOMS  (A**2):  2855 ; 1.206 ; 3.000       
REMARK   3   MAIN-CHAIN BOND OTHER ATOMS    (A**2):  1118 ; 0.262 ; 3.000       
REMARK   3   MAIN-CHAIN ANGLE REFINED ATOMS (A**2):  4453 ; 2.007 ; 5.000       
REMARK   3   SIDE-CHAIN BOND REFINED ATOMS  (A**2):  1460 ; 3.516 ; 7.000       
REMARK   3   SIDE-CHAIN ANGLE REFINED ATOMS (A**2):  1220 ; 4.779 ;11.000       
REMARK   3                                                                      
REMARK   3 ANISOTROPIC THERMAL FACTOR RESTRAINTS.    COUNT   RMS   WEIGHT       
REMARK   3   RIGID-BOND RESTRAINTS          (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   SPHERICITY; FREE ATOMS         (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   SPHERICITY; BONDED ATOMS       (A**2):  NULL ;  NULL ;  NULL       
REMARK   3                                                                      
REMARK   3  NCS RESTRAINTS STATISTICS                                           
REMARK   3   NUMBER OF DIFFERENT NCS GROUPS : NULL                              
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : 20                                         
REMARK   3                                                                      
REMARK   3   TLS GROUP : 1                                                      
REMARK   3    NUMBER OF COMPONENTS GROUP : 1                                    
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI                         
REMARK   3    RESIDUE RANGE :   A    13        A    48                          
REMARK   3    ORIGIN FOR THE GROUP (A):  48.7980  18.1580  35.4080              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.0977 T22:   0.2494                                     
REMARK   3      T33:   0.0108 T12:  -0.1568                                     
REMARK   3      T13:  -0.0152 T23:  -0.0075                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   8.6241 L22:  11.3744                                     
REMARK   3      L33:   7.8195 L12:   0.9267                                     
REMARK   3      L13:   1.4581 L23:   0.8084                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.2784 S12:  -0.2377 S13:  -0.2010                       
REMARK   3      S21:   0.5499 S22:   0.0010 S23:  -0.7420                       
REMARK   3      S31:  -0.5739 S32:   1.3427 S33:  -0.2794                       
REMARK   3                                                                      
REMARK   3   TLS GROUP : 2                                                      
REMARK   3    NUMBER OF COMPONENTS GROUP : 1                                    
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI                         
REMARK   3    RESIDUE RANGE :   A    49        A    62                          
REMARK   3    ORIGIN FOR THE GROUP (A):  35.2240  21.7710  20.9250              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2477 T22:   0.2907                                     
REMARK   3      T33:   0.0336 T12:   0.0372                                     
REMARK   3      T13:   0.0237 T23:   0.1014                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:  16.4995 L22:   1.3609                                     
REMARK   3      L33:   7.9029 L12:   0.7409                                     
REMARK   3      L13:   3.9476 L23:  -2.8622                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.5378 S12:   1.5136 S13:   0.5229                       
REMARK   3      S21:  -0.2523 S22:   0.0788 S23:   0.5509                       
REMARK   3      S31:  -0.2387 S32:   0.1790 S33:  -0.6166                       
REMARK   3                                                                      
REMARK   3   TLS GROUP : 3                                                      
REMARK   3    NUMBER OF COMPONENTS GROUP : 1                                    
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI                         
REMARK   3    RESIDUE RANGE :   A    63        A    86                          
REMARK   3    ORIGIN FOR THE GROUP (A):  32.6110  14.1230  33.4390              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.1806 T22:   0.0720                                     
REMARK   3      T33:   0.1694 T12:  -0.0137                                     
REMARK   3      T13:   0.0703 T23:  -0.0009                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   3.2057 L22:   8.9830                                     
REMARK   3      L33:   1.4326 L12:   4.0614                                     
REMARK   3      L13:   0.6625 L23:   3.0692                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0366 S12:   0.0904 S13:   0.6843                       
REMARK   3      S21:   0.2710 S22:  -0.1080 S23:   1.1381                       
REMARK   3      S31:  -0.1543 S32:  -0.0804 S33:   0.0714                       
REMARK   3                                                                      
REMARK   3   TLS GROUP : 4                                                      
REMARK   3    NUMBER OF COMPONENTS GROUP : 1                                    
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI                         
REMARK   3    RESIDUE RANGE :   A    87        A   113                          
REMARK   3    ORIGIN FOR THE GROUP (A):  39.9450  19.6580  31.5650              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2564 T22:   0.1763                                     
REMARK   3      T33:   0.0964 T12:  -0.0700                                     
REMARK   3      T13:   0.0615 T23:  -0.0400                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.8565 L22:  13.7974                                     
REMARK   3      L33:   3.0296 L12:   0.3508                                     
REMARK   3      L13:  -1.0577 L23:  -1.2619                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.2010 S12:   0.3012 S13:   0.3250                       
REMARK   3      S21:   0.6091 S22:   0.0944 S23:   0.5811                       
REMARK   3      S31:  -0.7580 S32:   0.0434 S33:  -0.2954                       
REMARK   3                                                                      
REMARK   3   TLS GROUP : 5                                                      
REMARK   3    NUMBER OF COMPONENTS GROUP : 1                                    
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI                         
REMARK   3    RESIDUE RANGE :   A   114        A   152                          
REMARK   3    ORIGIN FOR THE GROUP (A):  34.0530  -3.7100  29.8860              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2075 T22:   0.1352                                     
REMARK   3      T33:   0.0945 T12:  -0.0401                                     
REMARK   3      T13:  -0.0074 T23:  -0.0367                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   3.9575 L22:   3.3692                                     
REMARK   3      L33:   2.9020 L12:   0.2103                                     
REMARK   3      L13:   0.4158 L23:   0.8864                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.1119 S12:  -0.0511 S13:   0.0898                       
REMARK   3      S21:   0.3423 S22:   0.0572 S23:   0.0153                       
REMARK   3      S31:   0.1083 S32:   0.0199 S33:  -0.1691                       
REMARK   3                                                                      
REMARK   3   TLS GROUP : 6                                                      
REMARK   3    NUMBER OF COMPONENTS GROUP : 1                                    
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI                         
REMARK   3    RESIDUE RANGE :   A   153        A   193                          
REMARK   3    ORIGIN FOR THE GROUP (A):  35.5440   4.4610  25.9330              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.1393 T22:   0.1668                                     
REMARK   3      T33:   0.1575 T12:  -0.0643                                     
REMARK   3      T13:   0.0214 T23:  -0.0009                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.1849 L22:   2.1372                                     
REMARK   3      L33:   5.4804 L12:   0.4296                                     
REMARK   3      L13:  -0.4787 L23:   0.5854                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.1505 S12:   0.1762 S13:   0.2274                       
REMARK   3      S21:   0.0349 S22:   0.2834 S23:  -0.0688                       
REMARK   3      S31:  -0.2151 S32:  -0.0600 S33:  -0.1329                       
REMARK   3                                                                      
REMARK   3   TLS GROUP : 7                                                      
REMARK   3    NUMBER OF COMPONENTS GROUP : 1                                    
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI                         
REMARK   3    RESIDUE RANGE :   A   194        A   212                          
REMARK   3    ORIGIN FOR THE GROUP (A):  26.1050   0.9270  14.4570              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2412 T22:   0.1005                                     
REMARK   3      T33:   0.1774 T12:   0.0020                                     
REMARK   3      T13:  -0.0094 T23:   0.0323                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.0148 L22:   1.8632                                     
REMARK   3      L33:   7.7334 L12:   1.3751                                     
REMARK   3      L13:  -1.3705 L23:  -1.8487                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.1053 S12:   0.0129 S13:   0.9227                       
REMARK   3      S21:  -0.0624 S22:   0.2486 S23:   0.3201                       
REMARK   3      S31:  -1.2395 S32:  -0.5107 S33:  -0.3539                       
REMARK   3                                                                      
REMARK   3   TLS GROUP : 8                                                      
REMARK   3    NUMBER OF COMPONENTS GROUP : 1                                    
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI                         
REMARK   3    RESIDUE RANGE :   A   213        A   258                          
REMARK   3    ORIGIN FOR THE GROUP (A):  28.5630  -8.0380  13.7360              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.1506 T22:   0.1320                                     
REMARK   3      T33:   0.0998 T12:  -0.0697                                     
REMARK   3      T13:  -0.0197 T23:   0.0161                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   3.5106 L22:   1.0399                                     
REMARK   3      L33:   4.6740 L12:   0.3431                                     
REMARK   3      L13:   1.0255 L23:   0.7853                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0870 S12:   0.3257 S13:  -0.0044                       
REMARK   3      S21:  -0.0195 S22:   0.1030 S23:   0.0924                       
REMARK   3      S31:   0.0571 S32:  -0.1169 S33:  -0.1899                       
REMARK   3                                                                      
REMARK   3   TLS GROUP : 9                                                      
REMARK   3    NUMBER OF COMPONENTS GROUP : 1                                    
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI                         
REMARK   3    RESIDUE RANGE :   A   259        A   287                          
REMARK   3    ORIGIN FOR THE GROUP (A):  28.3270 -14.7080   7.7630              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2021 T22:   0.0993                                     
REMARK   3      T33:   0.0943 T12:  -0.0272                                     
REMARK   3      T13:  -0.0123 T23:  -0.0466                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.1058 L22:   1.8672                                     
REMARK   3      L33:   4.2675 L12:   0.3560                                     
REMARK   3      L13:  -0.1213 L23:  -0.1996                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.2281 S12:   0.1064 S13:  -0.0930                       
REMARK   3      S21:  -0.0748 S22:   0.1612 S23:   0.1641                       
REMARK   3      S31:   0.3361 S32:  -0.3080 S33:  -0.3893                       
REMARK   3                                                                      
REMARK   3   TLS GROUP : 10                                                     
REMARK   3    NUMBER OF COMPONENTS GROUP : 1                                    
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI                         
REMARK   3    RESIDUE RANGE :   A   288        A   321                          
REMARK   3    ORIGIN FOR THE GROUP (A):  24.1380 -11.9180  28.0290              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.1869 T22:   0.1586                                     
REMARK   3      T33:   0.1151 T12:  -0.1365                                     
REMARK   3      T13:   0.0011 T23:  -0.0037                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   3.1933 L22:   3.3569                                     
REMARK   3      L33:   3.9888 L12:  -0.4608                                     
REMARK   3      L13:   0.7688 L23:  -0.1757                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.3359 S12:  -0.3358 S13:  -0.1234                       
REMARK   3      S21:   0.3772 S22:  -0.1514 S23:   0.1772                       
REMARK   3      S31:   0.4389 S32:  -0.4924 S33:  -0.1845                       
REMARK   3                                                                      
REMARK   3   TLS GROUP : 11                                                     
REMARK   3    NUMBER OF COMPONENTS GROUP : 1                                    
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI                         
REMARK   3    RESIDUE RANGE :   B    21        B    53                          
REMARK   3    ORIGIN FOR THE GROUP (A):  12.9180  48.0760  38.0910              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2685 T22:   0.0945                                     
REMARK   3      T33:   0.0434 T12:   0.0213                                     
REMARK   3      T13:   0.1324 T23:   0.0526                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:  12.8005 L22:   7.1371                                     
REMARK   3      L33:  20.1155 L12:  -1.8734                                     
REMARK   3      L13:   1.4792 L23:   1.3744                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.4879 S12:  -0.2622 S13:   1.1716                       
REMARK   3      S21:  -1.1458 S22:   0.0784 S23:  -0.3554                       
REMARK   3      S31:  -0.7206 S32:  -0.1283 S33:  -0.5663                       
REMARK   3                                                                      
REMARK   3   TLS GROUP : 12                                                     
REMARK   3    NUMBER OF COMPONENTS GROUP : 1                                    
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI                         
REMARK   3    RESIDUE RANGE :   B    54        B    72                          
REMARK   3    ORIGIN FOR THE GROUP (A):  24.8410  37.8590  46.8370              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2430 T22:   0.3371                                     
REMARK   3      T33:   0.8717 T12:  -0.1964                                     
REMARK   3      T13:   0.3555 T23:   0.0120                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:  16.3455 L22:  15.7102                                     
REMARK   3      L33:  20.6103 L12: -12.6631                                     
REMARK   3      L13: -16.9329 L23:   8.8630                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.2652 S12:  -1.9086 S13:   1.6729                       
REMARK   3      S21:   1.6421 S22:   1.0862 S23:  -0.4120                       
REMARK   3      S31:  -0.5100 S32:   1.0065 S33:  -1.3514                       
REMARK   3                                                                      
REMARK   3   TLS GROUP : 13                                                     
REMARK   3    NUMBER OF COMPONENTS GROUP : 1                                    
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI                         
REMARK   3    RESIDUE RANGE :   B    73        B    91                          
REMARK   3    ORIGIN FOR THE GROUP (A):  20.7290  40.8150  36.0290              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2567 T22:   0.2544                                     
REMARK   3      T33:   0.1702 T12:  -0.0257                                     
REMARK   3      T13:   0.1073 T23:   0.0747                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   5.9950 L22:   9.7844                                     
REMARK   3      L33:   4.5142 L12:   5.0947                                     
REMARK   3      L13:  -1.8187 L23:   1.7612                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.4230 S12:  -0.0990 S13:   0.7713                       
REMARK   3      S21:   0.0228 S22:  -0.1037 S23:  -0.7697                       
REMARK   3      S31:  -0.6065 S32:   1.2220 S33:  -0.3194                       
REMARK   3                                                                      
REMARK   3   TLS GROUP : 14                                                     
REMARK   3    NUMBER OF COMPONENTS GROUP : 1                                    
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI                         
REMARK   3    RESIDUE RANGE :   B    92        B   110                          
REMARK   3    ORIGIN FOR THE GROUP (A):  18.4720  43.5620  38.5180              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.1695 T22:   0.3723                                     
REMARK   3      T33:   0.1177 T12:   0.0280                                     
REMARK   3      T13:   0.1216 T23:   0.0874                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:  21.6612 L22:   6.1984                                     
REMARK   3      L33:  24.9139 L12:   5.6632                                     
REMARK   3      L13:  11.5865 L23:   6.0756                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.6139 S12:  -0.5229 S13:   0.4159                       
REMARK   3      S21:  -0.6418 S22:  -0.0488 S23:  -0.8135                       
REMARK   3      S31:   0.6937 S32:   1.7866 S33:  -0.5651                       
REMARK   3                                                                      
REMARK   3   TLS GROUP : 15                                                     
REMARK   3    NUMBER OF COMPONENTS GROUP : 1                                    
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI                         
REMARK   3    RESIDUE RANGE :   B   111        B   170                          
REMARK   3    ORIGIN FOR THE GROUP (A):   6.6480  28.0110  37.6810              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2417 T22:   0.1640                                     
REMARK   3      T33:   0.0436 T12:   0.1133                                     
REMARK   3      T13:   0.0039 T23:   0.0362                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   4.8220 L22:   3.0167                                     
REMARK   3      L33:   1.8483 L12:  -1.0897                                     
REMARK   3      L13:  -0.2981 L23:  -0.7941                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.3417 S12:   0.4401 S13:   0.2479                       
REMARK   3      S21:  -0.4151 S22:  -0.3043 S23:  -0.1027                       
REMARK   3      S31:  -0.1928 S32:  -0.0889 S33:  -0.0373                       
REMARK   3                                                                      
REMARK   3   TLS GROUP : 16                                                     
REMARK   3    NUMBER OF COMPONENTS GROUP : 1                                    
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI                         
REMARK   3    RESIDUE RANGE :   B   171        B   197                          
REMARK   3    ORIGIN FOR THE GROUP (A):   9.6990  30.9020  54.7950              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3184 T22:   0.1272                                     
REMARK   3      T33:   0.1158 T12:   0.0527                                     
REMARK   3      T13:  -0.0382 T23:  -0.0818                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.7136 L22:   4.1743                                     
REMARK   3      L33:   2.4250 L12:  -0.6505                                     
REMARK   3      L13:  -0.3462 L23:   3.1761                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0624 S12:  -0.2816 S13:   0.5512                       
REMARK   3      S21:   0.2051 S22:   0.2630 S23:  -0.5853                       
REMARK   3      S31:   0.3512 S32:   0.0261 S33:  -0.3254                       
REMARK   3                                                                      
REMARK   3   TLS GROUP : 17                                                     
REMARK   3    NUMBER OF COMPONENTS GROUP : 1                                    
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI                         
REMARK   3    RESIDUE RANGE :   B   198        B   236                          
REMARK   3    ORIGIN FOR THE GROUP (A):   8.0210  20.4850  50.2880              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2107 T22:   0.1171                                     
REMARK   3      T33:   0.1137 T12:  -0.0064                                     
REMARK   3      T13:  -0.0535 T23:  -0.0156                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.9032 L22:   2.5757                                     
REMARK   3      L33:   3.4772 L12:  -1.7559                                     
REMARK   3      L13:   0.7000 L23:  -1.7733                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0641 S12:  -0.1382 S13:   0.0831                       
REMARK   3      S21:   0.1724 S22:  -0.0386 S23:  -0.0705                       
REMARK   3      S31:  -0.3455 S32:   0.0344 S33:  -0.0255                       
REMARK   3                                                                      
REMARK   3   TLS GROUP : 18                                                     
REMARK   3    NUMBER OF COMPONENTS GROUP : 1                                    
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI                         
REMARK   3    RESIDUE RANGE :   B   237        B   261                          
REMARK   3    ORIGIN FOR THE GROUP (A):   7.2120  10.6520  59.0020              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.1236 T22:   0.1744                                     
REMARK   3      T33:   0.1310 T12:  -0.0270                                     
REMARK   3      T13:  -0.0216 T23:   0.0618                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   5.9504 L22:   4.9257                                     
REMARK   3      L33:   7.2514 L12:  -2.7901                                     
REMARK   3      L13:   2.5604 L23:  -0.5964                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0932 S12:  -0.5087 S13:  -0.5354                       
REMARK   3      S21:   0.1985 S22:   0.3034 S23:   0.0639                       
REMARK   3      S31:  -0.0345 S32:  -0.1472 S33:  -0.2102                       
REMARK   3                                                                      
REMARK   3   TLS GROUP : 19                                                     
REMARK   3    NUMBER OF COMPONENTS GROUP : 1                                    
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI                         
REMARK   3    RESIDUE RANGE :   B   262        B   293                          
REMARK   3    ORIGIN FOR THE GROUP (A):  -1.1310  10.9690  49.0690              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.0991 T22:   0.0096                                     
REMARK   3      T33:   0.1570 T12:  -0.1002                                     
REMARK   3      T13:  -0.0848 T23:   0.0086                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   4.6941 L22:   2.0267                                     
REMARK   3      L33:  10.7516 L12:  -2.0169                                     
REMARK   3      L13:   1.8304 L23:  -2.5141                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.1027 S12:  -0.2330 S13:  -0.4905                       
REMARK   3      S21:  -0.0058 S22:   0.0647 S23:   0.4880                       
REMARK   3      S31:   0.6205 S32:  -0.7502 S33:  -0.1675                       
REMARK   3                                                                      
REMARK   3   TLS GROUP : 20                                                     
REMARK   3    NUMBER OF COMPONENTS GROUP : 1                                    
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI                         
REMARK   3    RESIDUE RANGE :   B   294        B   320                          
REMARK   3    ORIGIN FOR THE GROUP (A):   9.1350  12.3710  38.7520              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2089 T22:   0.0900                                     
REMARK   3      T33:   0.0974 T12:   0.0944                                     
REMARK   3      T13:  -0.1048 T23:  -0.0803                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   4.4571 L22:   4.7745                                     
REMARK   3      L33:   8.3280 L12:  -3.0738                                     
REMARK   3      L13:   1.0409 L23:  -4.5065                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.6175 S12:   0.5337 S13:  -0.5355                       
REMARK   3      S21:  -0.8408 S22:  -0.4472 S23:   0.1052                       
REMARK   3      S31:   0.8436 S32:   0.1145 S33:  -0.1703                       
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED : MASK                                                 
REMARK   3   PARAMETERS FOR MASK CALCULATION                                    
REMARK   3   VDW PROBE RADIUS   : 1.40                                          
REMARK   3   ION PROBE RADIUS   : 0.80                                          
REMARK   3   SHRINKAGE RADIUS   : 0.80                                          
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: HYDROGENS HAVE BEEN ADDED IN THE          
REMARK   3  RIDING POSITIONS                                                    
REMARK   4                                                                      
REMARK   4 2I6L COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 30-AUG-06.                  
REMARK 100 THE RCSB ID CODE IS RCSB039201.                                      
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 12-JUL-06                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 6.7                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : SLS                                
REMARK 200  BEAMLINE                       : X10SA                              
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.9791                             
REMARK 200  MONOCHROMATOR                  : SI(111)                            
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : MARMOSAIC 225 MM CCD               
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : HKL-2000                           
REMARK 200  DATA SCALING SOFTWARE          : HKL-2000                           
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 31022                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.250                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 50.000                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.7                               
REMARK 200  DATA REDUNDANCY                : 8.000                              
REMARK 200  R MERGE                    (I) : 0.10100                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 8.2000                             
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.25                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.33                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 99.6                               
REMARK 200  DATA REDUNDANCY IN SHELL       : 7.80                               
REMARK 200  R MERGE FOR SHELL          (I) : NULL                               
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : NULL                               
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: PHASER                                                
REMARK 200 STARTING MODEL: 1ERK                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 40.40                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.02                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 1.8M MGSO4; 0.1M MES, PH 6.7, VAPOR      
REMARK 280  DIFFUSION, SITTING DROP, TEMPERATURE 277K                           
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 43 21 2                        
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,-Y,Z+1/2                                             
REMARK 290       3555   -Y+1/2,X+1/2,Z+3/4                                      
REMARK 290       4555   Y+1/2,-X+1/2,Z+1/4                                      
REMARK 290       5555   -X+1/2,Y+1/2,-Z+3/4                                     
REMARK 290       6555   X+1/2,-Y+1/2,-Z+1/4                                     
REMARK 290       7555   Y,X,-Z                                                  
REMARK 290       8555   -Y,-X,-Z+1/2                                            
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       90.58750            
REMARK 290   SMTRY1   3  0.000000 -1.000000  0.000000       41.56000            
REMARK 290   SMTRY2   3  1.000000  0.000000  0.000000       41.56000            
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000      135.88125            
REMARK 290   SMTRY1   4  0.000000  1.000000  0.000000       41.56000            
REMARK 290   SMTRY2   4 -1.000000  0.000000  0.000000       41.56000            
REMARK 290   SMTRY3   4  0.000000  0.000000  1.000000       45.29375            
REMARK 290   SMTRY1   5 -1.000000  0.000000  0.000000       41.56000            
REMARK 290   SMTRY2   5  0.000000  1.000000  0.000000       41.56000            
REMARK 290   SMTRY3   5  0.000000  0.000000 -1.000000      135.88125            
REMARK 290   SMTRY1   6  1.000000  0.000000  0.000000       41.56000            
REMARK 290   SMTRY2   6  0.000000 -1.000000  0.000000       41.56000            
REMARK 290   SMTRY3   6  0.000000  0.000000 -1.000000       45.29375            
REMARK 290   SMTRY1   7  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY2   7  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY3   7  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   8  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY2   8 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY3   8  0.000000  0.000000 -1.000000       90.58750            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC                    
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 1500 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 26320 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -11.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     SER A     8                                                      
REMARK 465     MET A     9                                                      
REMARK 465     ASN A    10                                                      
REMARK 465     ILE A    11                                                      
REMARK 465     HIS A    12                                                      
REMARK 465     GLY A    27                                                      
REMARK 465     CYS A    28                                                      
REMARK 465     GLY A    29                                                      
REMARK 465     GLY A    30                                                      
REMARK 465     ASN A    31                                                      
REMARK 465     ASP A    94                                                      
REMARK 465     VAL A    95                                                      
REMARK 465     GLY A    96                                                      
REMARK 465     SER A    97                                                      
REMARK 465     ASP A   179                                                      
REMARK 465     PRO A   180                                                      
REMARK 465     HIS A   181                                                      
REMARK 465     TYR A   182                                                      
REMARK 465     SER A   183                                                      
REMARK 465     HIS A   184                                                      
REMARK 465     LYS A   185                                                      
REMARK 465     GLY A   186                                                      
REMARK 465     PRO A   322                                                      
REMARK 465     MET A   323                                                      
REMARK 465     ASP A   324                                                      
REMARK 465     GLU A   325                                                      
REMARK 465     PRO A   326                                                      
REMARK 465     ILE A   327                                                      
REMARK 465     SER B     8                                                      
REMARK 465     MET B     9                                                      
REMARK 465     ASN B    10                                                      
REMARK 465     ILE B    11                                                      
REMARK 465     HIS B    12                                                      
REMARK 465     GLY B    13                                                      
REMARK 465     PHE B    14                                                      
REMARK 465     ASP B    15                                                      
REMARK 465     LEU B    16                                                      
REMARK 465     GLY B    17                                                      
REMARK 465     SER B    18                                                      
REMARK 465     ARG B    19                                                      
REMARK 465     TYR B    20                                                      
REMARK 465     GLY B    27                                                      
REMARK 465     CYS B    28                                                      
REMARK 465     GLY B    29                                                      
REMARK 465     ASP B    94                                                      
REMARK 465     VAL B    95                                                      
REMARK 465     GLY B    96                                                      
REMARK 465     SER B    97                                                      
REMARK 465     LEU B    98                                                      
REMARK 465     THR B    99                                                      
REMARK 465     GLU B   100                                                      
REMARK 465     LEU B   101                                                      
REMARK 465     ASN B   102                                                      
REMARK 465     GLY B   173                                                      
REMARK 465     LEU B   174                                                      
REMARK 465     ALA B   175                                                      
REMARK 465     ARG B   176                                                      
REMARK 465     ILE B   177                                                      
REMARK 465     MET B   178                                                      
REMARK 465     ASP B   179                                                      
REMARK 465     PRO B   180                                                      
REMARK 465     HIS B   181                                                      
REMARK 465     TYR B   182                                                      
REMARK 465     SER B   183                                                      
REMARK 465     HIS B   184                                                      
REMARK 465     LYS B   185                                                      
REMARK 465     GLY B   186                                                      
REMARK 465     TYR B   266                                                      
REMARK 465     ILE B   267                                                      
REMARK 465     ARG B   268                                                      
REMARK 465     ASN B   269                                                      
REMARK 465     ASP B   270                                                      
REMARK 465     PHE B   321                                                      
REMARK 465     PRO B   322                                                      
REMARK 465     MET B   323                                                      
REMARK 465     ASP B   324                                                      
REMARK 465     GLU B   325                                                      
REMARK 465     PRO B   326                                                      
REMARK 465     ILE B   327                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS(M=MODEL NUMBER;            
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     PHE A  14    CG   CD1  CD2  CE1  CE2  CZ                         
REMARK 470     ASP A  15    CG   OD1  OD2                                       
REMARK 470     MET A  21    SD   CE                                             
REMARK 470     LEU A  23    CD1  CD2                                            
REMARK 470     LYS A  24    CD   CE   NZ                                        
REMARK 470     LEU A  26    CG   CD1  CD2                                       
REMARK 470     LEU A  33    CG   CD1  CD2                                       
REMARK 470     ASP A  41    CG   OD1  OD2                                       
REMARK 470     ASP A  43    CG   OD1  OD2                                       
REMARK 470     LYS A  44    CG   CD   CE   NZ                                   
REMARK 470     ARG A  45    NE   CZ   NH1  NH2                                  
REMARK 470     THR A  54    OG1  CG2                                            
REMARK 470     ASP A  55    CG   OD1  OD2                                       
REMARK 470     GLN A  57    CG   CD   OE1  NE2                                  
REMARK 470     LYS A  60    CG   CD   CE   NZ                                   
REMARK 470     ARG A  70    CD   NE   CZ   NH1  NH2                             
REMARK 470     ARG A  71    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ASP A  73    CG   OD1  OD2                                       
REMARK 470     GLU A  82    CD   OE1  OE2                                       
REMARK 470     GLN A  90    CG   CD   OE1  NE2                                  
REMARK 470     THR A  92    OG1  CG2                                            
REMARK 470     ASP A  93    CG   OD1  OD2                                       
REMARK 470     LEU A  98    CG   CD1  CD2                                       
REMARK 470     THR A  99    OG1  CG2                                            
REMARK 470     GLU A 100    CG   CD   OE1  OE2                                  
REMARK 470     GLU A 120    CD   OE1  OE2                                       
REMARK 470     GLN A 121    CD   OE1  NE2                                       
REMARK 470     GLU A 127    CD   OE1  OE2                                       
REMARK 470     ARG A 138    CD   NE   CZ   NH1  NH2                             
REMARK 470     ASN A 147    OD1  ND2                                            
REMARK 470     ARG A 151    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     LYS A 154    NZ                                                  
REMARK 470     GLU A 163    CG   CD   OE1  OE2                                  
REMARK 470     ASP A 164    CG   OD1  OD2                                       
REMARK 470     ARG A 176    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ILE A 177    CG1  CD1                                            
REMARK 470     HIS A 187    CG   ND1  CD2  CE1  NE2                             
REMARK 470     LEU A 188    CG   CD1  CD2                                       
REMARK 470     GLU A 190    CG   CD   OE1  OE2                                  
REMARK 470     ARG A 198    NH1  NH2                                            
REMARK 470     ARG A 201    CD   NE   CZ   NH1  NH2                             
REMARK 470     TYR A 209    CG   CD1  CD2  CE1  CE2  CZ   OH                    
REMARK 470     LYS A 211    CG   CD   CE   NZ                                   
REMARK 470     LYS A 229    CE   NZ                                             
REMARK 470     GLU A 253    CG   CD   OE1  OE2                                  
REMARK 470     GLU A 254    OE1  OE2                                            
REMARK 470     ARG A 256    NE   CZ   NH1  NH2                                  
REMARK 470     GLN A 257    CD   OE1  NE2                                       
REMARK 470     GLU A 258    CD   OE1  OE2                                       
REMARK 470     LEU A 260    CG   CD1  CD2                                       
REMARK 470     ASN A 269    OD1  ND2                                            
REMARK 470     GLU A 273    OE1  OE2                                            
REMARK 470     ARG A 287    NE   CZ   NH1  NH2                                  
REMARK 470     GLU A 288    CG   CD   OE1  OE2                                  
REMARK 470     ASP A 291    OD1  OD2                                            
REMARK 470     GLU A 294    CD   OE1  OE2                                       
REMARK 470     ILE A 296    CD1                                                 
REMARK 470     MET A 302    SD   CE                                             
REMARK 470     GLU A 308    CG   CD   OE1  OE2                                  
REMARK 470     PHE A 321    CG   CD1  CD2  CE1  CE2  CZ                         
REMARK 470     MET B  21    CG   SD   CE                                        
REMARK 470     ASP B  22    CG   OD1  OD2                                       
REMARK 470     LYS B  24    CG   CD   CE   NZ                                   
REMARK 470     LEU B  26    CG   CD1  CD2                                       
REMARK 470     ASN B  40    CG   OD1  ND2                                       
REMARK 470     ASP B  41    CG   OD1  OD2                                       
REMARK 470     CYS B  42    SG                                                  
REMARK 470     ASP B  43    CG   OD1  OD2                                       
REMARK 470     LYS B  44    CG   CD   CE   NZ                                   
REMARK 470     ARG B  45    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     LYS B  49    CE   NZ                                             
REMARK 470     LYS B  50    CE   NZ                                             
REMARK 470     ASP B  55    CG   OD1  OD2                                       
REMARK 470     GLN B  57    CG   CD   OE1  NE2                                  
REMARK 470     VAL B  59    CG1  CG2                                            
REMARK 470     LYS B  60    CG   CD   CE   NZ                                   
REMARK 470     HIS B  61    CG   ND1  CD2  CE1  NE2                             
REMARK 470     LEU B  63    CG   CD1  CD2                                       
REMARK 470     ARG B  64    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     GLU B  65    CG   CD   OE1  OE2                                  
REMARK 470     ILE B  66    CG1  CG2  CD1                                       
REMARK 470     LYS B  67    CG   CD   CE   NZ                                   
REMARK 470     ILE B  68    CG1  CG2  CD1                                       
REMARK 470     ILE B  69    CG1  CG2  CD1                                       
REMARK 470     ARG B  70    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ARG B  71    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ASP B  75    OD1  OD2                                            
REMARK 470     LYS B  79    CG   CD   CE   NZ                                   
REMARK 470     GLU B  82    CG   CD   OE1  OE2                                  
REMARK 470     LEU B  91    CG   CD1  CD2                                       
REMARK 470     THR B  92    OG1  CG2                                            
REMARK 470     ASP B  93    CG   OD1  OD2                                       
REMARK 470     SER B 103    OG                                                  
REMARK 470     TYR B 105    CG   CD1  CD2  CE1  CE2  CZ   OH                    
REMARK 470     ARG B 138    CD   NE   CZ   NH1  NH2                             
REMARK 470     LYS B 141    CD   CE   NZ                                        
REMARK 470     ARG B 151    CZ   NH1  NH2                                       
REMARK 470     GLU B 163    CG   CD   OE1  OE2                                  
REMARK 470     LYS B 168    CD   CE   NZ                                        
REMARK 470     HIS B 187    CG   ND1  CD2  CE1  NE2                             
REMARK 470     ARG B 198    CZ   NH1  NH2                                       
REMARK 470     ASN B 207    OD1  ND2                                            
REMARK 470     LYS B 211    CE   NZ                                             
REMARK 470     LYS B 229    CE   NZ                                             
REMARK 470     HIS B 236    ND1  CD2  CE1  NE2                                  
REMARK 470     MET B 241    CE                                                  
REMARK 470     GLU B 246    CD   OE1  OE2                                       
REMARK 470     ARG B 256    CD   NE   CZ   NH1  NH2                             
REMARK 470     GLN B 257    CD   OE1  NE2                                       
REMARK 470     GLU B 258    CD   OE1  OE2                                       
REMARK 470     VAL B 265    CG1  CG2                                            
REMARK 470     MET B 271    SD   CE                                             
REMARK 470     GLU B 273    CG   CD   OE1  OE2                                  
REMARK 470     HIS B 275    CG   ND1  CD2  CE1  NE2                             
REMARK 470     LYS B 276    CE   NZ                                             
REMARK 470     GLN B 280    CG   CD   OE1  NE2                                  
REMARK 470     ARG B 287    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     GLU B 288    CG   CD   OE1  OE2                                  
REMARK 470     GLU B 308    CD   OE1  OE2                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    ARG A 151       -9.76     77.86                                   
REMARK 500    ASP A 171       73.61     50.75                                   
REMARK 500    ILE A 177       75.08   -102.86                                   
REMARK 500    LEU A 282       57.29   -115.78                                   
REMARK 500    ASN B  31      175.61    -58.75                                   
REMARK 500    THR B  54      -32.73   -134.50                                   
REMARK 500    GLU B  65      -71.59    -60.22                                   
REMARK 500    ILE B  66      -70.68    -66.32                                   
REMARK 500    ARG B 151      -13.04     76.97                                   
REMARK 500    ASP B 171       70.35     43.53                                   
REMARK 500    ALA B 233       66.31   -108.60                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
DBREF  2I6L A    9   327  UNP    Q16659   MK06_HUMAN       9    327             
DBREF  2I6L B    9   327  UNP    Q16659   MK06_HUMAN       9    327             
SEQADV 2I6L SER A    8  UNP  Q16659              CLONING ARTIFACT               
SEQADV 2I6L VAL A  290  UNP  Q16659    LEU   290 CONFLICT                       
SEQADV 2I6L SER B    8  UNP  Q16659              CLONING ARTIFACT               
SEQADV 2I6L VAL B  290  UNP  Q16659    LEU   290 CONFLICT                       
SEQRES   1 A  320  SER MET ASN ILE HIS GLY PHE ASP LEU GLY SER ARG TYR          
SEQRES   2 A  320  MET ASP LEU LYS PRO LEU GLY CYS GLY GLY ASN GLY LEU          
SEQRES   3 A  320  VAL PHE SER ALA VAL ASP ASN ASP CYS ASP LYS ARG VAL          
SEQRES   4 A  320  ALA ILE LYS LYS ILE VAL LEU THR ASP PRO GLN SER VAL          
SEQRES   5 A  320  LYS HIS ALA LEU ARG GLU ILE LYS ILE ILE ARG ARG LEU          
SEQRES   6 A  320  ASP HIS ASP ASN ILE VAL LYS VAL PHE GLU ILE LEU GLY          
SEQRES   7 A  320  PRO SER GLY SER GLN LEU THR ASP ASP VAL GLY SER LEU          
SEQRES   8 A  320  THR GLU LEU ASN SER VAL TYR ILE VAL GLN GLU TYR MET          
SEQRES   9 A  320  GLU THR ASP LEU ALA ASN VAL LEU GLU GLN GLY PRO LEU          
SEQRES  10 A  320  LEU GLU GLU HIS ALA ARG LEU PHE MET TYR GLN LEU LEU          
SEQRES  11 A  320  ARG GLY LEU LYS TYR ILE HIS SER ALA ASN VAL LEU HIS          
SEQRES  12 A  320  ARG ASP LEU LYS PRO ALA ASN LEU PHE ILE ASN THR GLU          
SEQRES  13 A  320  ASP LEU VAL LEU LYS ILE GLY ASP PHE GLY LEU ALA ARG          
SEQRES  14 A  320  ILE MET ASP PRO HIS TYR SER HIS LYS GLY HIS LEU SER          
SEQRES  15 A  320  GLU GLY LEU VAL THR LYS TRP TYR ARG SER PRO ARG LEU          
SEQRES  16 A  320  LEU LEU SER PRO ASN ASN TYR THR LYS ALA ILE ASP MET          
SEQRES  17 A  320  TRP ALA ALA GLY CYS ILE PHE ALA GLU MET LEU THR GLY          
SEQRES  18 A  320  LYS THR LEU PHE ALA GLY ALA HIS GLU LEU GLU GLN MET          
SEQRES  19 A  320  GLN LEU ILE LEU GLU SER ILE PRO VAL VAL HIS GLU GLU          
SEQRES  20 A  320  ASP ARG GLN GLU LEU LEU SER VAL ILE PRO VAL TYR ILE          
SEQRES  21 A  320  ARG ASN ASP MET THR GLU PRO HIS LYS PRO LEU THR GLN          
SEQRES  22 A  320  LEU LEU PRO GLY ILE SER ARG GLU ALA VAL ASP PHE LEU          
SEQRES  23 A  320  GLU GLN ILE LEU THR PHE SER PRO MET ASP ARG LEU THR          
SEQRES  24 A  320  ALA GLU GLU ALA LEU SER HIS PRO TYR MET SER ILE TYR          
SEQRES  25 A  320  SER PHE PRO MET ASP GLU PRO ILE                              
SEQRES   1 B  320  SER MET ASN ILE HIS GLY PHE ASP LEU GLY SER ARG TYR          
SEQRES   2 B  320  MET ASP LEU LYS PRO LEU GLY CYS GLY GLY ASN GLY LEU          
SEQRES   3 B  320  VAL PHE SER ALA VAL ASP ASN ASP CYS ASP LYS ARG VAL          
SEQRES   4 B  320  ALA ILE LYS LYS ILE VAL LEU THR ASP PRO GLN SER VAL          
SEQRES   5 B  320  LYS HIS ALA LEU ARG GLU ILE LYS ILE ILE ARG ARG LEU          
SEQRES   6 B  320  ASP HIS ASP ASN ILE VAL LYS VAL PHE GLU ILE LEU GLY          
SEQRES   7 B  320  PRO SER GLY SER GLN LEU THR ASP ASP VAL GLY SER LEU          
SEQRES   8 B  320  THR GLU LEU ASN SER VAL TYR ILE VAL GLN GLU TYR MET          
SEQRES   9 B  320  GLU THR ASP LEU ALA ASN VAL LEU GLU GLN GLY PRO LEU          
SEQRES  10 B  320  LEU GLU GLU HIS ALA ARG LEU PHE MET TYR GLN LEU LEU          
SEQRES  11 B  320  ARG GLY LEU LYS TYR ILE HIS SER ALA ASN VAL LEU HIS          
SEQRES  12 B  320  ARG ASP LEU LYS PRO ALA ASN LEU PHE ILE ASN THR GLU          
SEQRES  13 B  320  ASP LEU VAL LEU LYS ILE GLY ASP PHE GLY LEU ALA ARG          
SEQRES  14 B  320  ILE MET ASP PRO HIS TYR SER HIS LYS GLY HIS LEU SER          
SEQRES  15 B  320  GLU GLY LEU VAL THR LYS TRP TYR ARG SER PRO ARG LEU          
SEQRES  16 B  320  LEU LEU SER PRO ASN ASN TYR THR LYS ALA ILE ASP MET          
SEQRES  17 B  320  TRP ALA ALA GLY CYS ILE PHE ALA GLU MET LEU THR GLY          
SEQRES  18 B  320  LYS THR LEU PHE ALA GLY ALA HIS GLU LEU GLU GLN MET          
SEQRES  19 B  320  GLN LEU ILE LEU GLU SER ILE PRO VAL VAL HIS GLU GLU          
SEQRES  20 B  320  ASP ARG GLN GLU LEU LEU SER VAL ILE PRO VAL TYR ILE          
SEQRES  21 B  320  ARG ASN ASP MET THR GLU PRO HIS LYS PRO LEU THR GLN          
SEQRES  22 B  320  LEU LEU PRO GLY ILE SER ARG GLU ALA VAL ASP PHE LEU          
SEQRES  23 B  320  GLU GLN ILE LEU THR PHE SER PRO MET ASP ARG LEU THR          
SEQRES  24 B  320  ALA GLU GLU ALA LEU SER HIS PRO TYR MET SER ILE TYR          
SEQRES  25 B  320  SER PHE PRO MET ASP GLU PRO ILE                              
FORMUL   3  HOH   *79(H2 O)                                                     
HELIX    1   1 ASP A   55  ARG A   71  1                                  17    
HELIX    2   2 LEU A  115  GLU A  120  1                                   6    
HELIX    3   3 LEU A  125  ALA A  146  1                                  22    
HELIX    4   4 LYS A  154  ALA A  156  5                                   3    
HELIX    5   5 SER A  189  VAL A  193  5                                   5    
HELIX    6   6 SER A  199  SER A  205  1                                   7    
HELIX    7   7 THR A  210  GLY A  228  1                                  19    
HELIX    8   8 HIS A  236  ILE A  248  1                                  13    
HELIX    9   9 HIS A  252  SER A  261  1                                  10    
HELIX   10  10 PRO A  264  MET A  271  1                                   8    
HELIX   11  11 PRO A  277  LEU A  282  1                                   6    
HELIX   12  12 SER A  286  GLN A  295  1                                  10    
HELIX   13  13 SER A  300  ARG A  304  5                                   5    
HELIX   14  14 THR A  306  SER A  312  1                                   7    
HELIX   15  15 HIS A  313  ILE A  318  1                                   6    
HELIX   16  16 ASP B   55  LYS B   60  1                                   6    
HELIX   17  17 HIS B   61  LEU B   72  1                                  12    
HELIX   18  18 LEU B  115  GLU B  120  1                                   6    
HELIX   19  19 LEU B  125  ALA B  146  1                                  22    
HELIX   20  20 LYS B  154  ALA B  156  5                                   3    
HELIX   21  21 SER B  189  VAL B  193  5                                   5    
HELIX   22  22 SER B  199  SER B  205  1                                   7    
HELIX   23  23 THR B  210  GLY B  228  1                                  19    
HELIX   24  24 HIS B  236  GLU B  246  1                                  11    
HELIX   25  25 HIS B  252  ILE B  263  1                                  12    
HELIX   26  26 PRO B  277  LEU B  282  1                                   6    
HELIX   27  27 SER B  286  GLN B  295  1                                  10    
HELIX   28  28 SER B  300  ARG B  304  5                                   5    
HELIX   29  29 THR B  306  HIS B  313  1                                   8    
HELIX   30  30 HIS B  313  ILE B  318  1                                   6    
SHEET    1   A 6 PHE A  14  LEU A  16  0                                        
SHEET    2   A 6 TYR A  20  PRO A  25 -1  O  TYR A  20   N  LEU A  16           
SHEET    3   A 6 VAL A  34  ASP A  39 -1  O  SER A  36   N  LYS A  24           
SHEET    4   A 6 ARG A  45  VAL A  52 -1  O  VAL A  46   N  ALA A  37           
SHEET    5   A 6 SER A 103  GLU A 109 -1  O  VAL A 104   N  ILE A  51           
SHEET    6   A 6 VAL A  80  LEU A  84 -1  N  GLU A  82   O  VAL A 107           
SHEET    1   B 3 THR A 113  ASP A 114  0                                        
SHEET    2   B 3 LEU A 158  ASN A 161 -1  O  ILE A 160   N  THR A 113           
SHEET    3   B 3 VAL A 166  ILE A 169 -1  O  LYS A 168   N  PHE A 159           
SHEET    1   C 5 LYS B  24  PRO B  25  0                                        
SHEET    2   C 5 VAL B  34  ASP B  39 -1  O  SER B  36   N  LYS B  24           
SHEET    3   C 5 LYS B  44  ILE B  51 -1  O  LYS B  44   N  ASP B  39           
SHEET    4   C 5 VAL B 104  GLU B 109 -1  O  VAL B 104   N  ILE B  51           
SHEET    5   C 5 VAL B  80  LEU B  84 -1  N  LEU B  84   O  TYR B 105           
SHEET    1   D 3 THR B 113  ASP B 114  0                                        
SHEET    2   D 3 LEU B 158  ASN B 161 -1  O  ILE B 160   N  THR B 113           
SHEET    3   D 3 VAL B 166  ILE B 169 -1  O  LYS B 168   N  PHE B 159           
CRYST1   83.120   83.120  181.175  90.00  90.00  90.00 P 43 21 2    16          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.012031  0.000000  0.000000        0.00000                         
SCALE2      0.000000  0.012031  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.005520        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
DBGET integrated database retrieval system