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Database: PDB
Entry: 2IF1
LinkDB: 2IF1
Original site: 2IF1 
HEADER    TRANSLATION INITIATION FACTOR           04-AUG-98   2IF1              
TITLE     HUMAN TRANSLATION INITIATION FACTOR EIF1, NMR, 29 STRUCTURES          
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: EIF1;                                                      
COMPND   3 CHAIN: A;                                                            
COMPND   4 SYNONYM: SUI1;                                                       
COMPND   5 ENGINEERED: YES;                                                     
COMPND   6 OTHER_DETAILS: N-TERMINAL POLY-HISTIDINE TAG                         
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 CELL_LINE: BL21;                                                     
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3);                       
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE   8 EXPRESSION_SYSTEM_STRAIN: BL21 (DE3);                                
SOURCE   9 EXPRESSION_SYSTEM_PLASMID: PQE31(HIS6-EIF1)                          
KEYWDS    TRANSLATION INITIATION FACTOR                                         
EXPDTA    SOLUTION NMR                                                          
NUMMDL    29                                                                    
AUTHOR    C.M.FLETCHER,C.U.T.HELLEN,T.V.PESTOVA,G.WAGNER                        
REVDAT   2   24-FEB-09 2IF1    1       VERSN                                    
REVDAT   1   18-MAY-99 2IF1    0                                                
JRNL        AUTH   C.M.FLETCHER,T.V.PESTOVA,C.U.HELLEN,G.WAGNER                 
JRNL        TITL   STRUCTURE AND INTERACTIONS OF THE TRANSLATION                
JRNL        TITL 2 INITIATION FACTOR EIF1.                                      
JRNL        REF    EMBO J.                       V.  18  2631 1999              
JRNL        REFN                   ISSN 0261-4189                               
JRNL        PMID   10228174                                                     
JRNL        DOI    10.1093/EMBOJ/18.9.2631                                      
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : X-PLOR 3.851                                         
REMARK   3   AUTHORS     : BRUNGER                                              
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 2IF1 COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY BNL.                                
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 300                                
REMARK 210  PH                             : 6.0                                
REMARK 210  IONIC STRENGTH                 : 400 MM                             
REMARK 210  PRESSURE                       : 1 ATM                              
REMARK 210  SAMPLE CONTENTS                : NULL                               
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : NULL                               
REMARK 210  SPECTROMETER FIELD STRENGTH    : 400 MHZ, 750 MHZ                   
REMARK 210  SPECTROMETER MODEL             : UNITY-PLUS, INOVA                  
REMARK 210  SPECTROMETER MANUFACTURER      : VARIAN                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : X-PLOR 3.851                       
REMARK 210   METHOD USED                   : SIMULATED ANNEALING                
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 30                                 
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 29                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : LOW RESTRAINT VIOLATIONS           
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: NULL                                                         
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
DBREF  2IF1 A   14   126  UNP    P41567   EIF1_HUMAN       1    113             
SEQRES   1 A  126  MET ARG GLY SER HIS HIS HIS HIS HIS HIS THR ASP PRO          
SEQRES   2 A  126  MET SER ALA ILE GLN ASN LEU HIS SER PHE ASP PRO PHE          
SEQRES   3 A  126  ALA ASP ALA SER LYS GLY ASP ASP LEU LEU PRO ALA GLY          
SEQRES   4 A  126  THR GLU ASP TYR ILE HIS ILE ARG ILE GLN GLN ARG ASN          
SEQRES   5 A  126  GLY ARG LYS THR LEU THR THR VAL GLN GLY ILE ALA ASP          
SEQRES   6 A  126  ASP TYR ASP LYS LYS LYS LEU VAL LYS ALA PHE LYS LYS          
SEQRES   7 A  126  LYS PHE ALA CYS ASN GLY THR VAL ILE GLU HIS PRO GLU          
SEQRES   8 A  126  TYR GLY GLU VAL ILE GLN LEU GLN GLY ASP GLN ARG LYS          
SEQRES   9 A  126  ASN ILE CYS GLN PHE LEU VAL GLU ILE GLY LEU ALA LYS          
SEQRES  10 A  126  ASP ASP GLN LEU LYS VAL HIS GLY PHE                          
HELIX    1  A1 LYS A   69  PHE A   80  1                                  12    
HELIX    2  A2 ILE A  106  ILE A  113  1                                   8    
SHEET    1  B1 5 GLN A 120  PHE A 126  0                                        
SHEET    2  B1 5 ILE A  44  GLN A  50  1                                        
SHEET    3  B1 5 THR A  56  GLN A  61 -1                                        
SHEET    4  B1 5 GLU A  94  GLN A  99 -1                                        
SHEET    5  B1 5 ASN A  83  GLU A  88 -1                                        
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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