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Database: PDB
Entry: 2K7V
LinkDB: 2K7V
Original site: 2K7V 
HEADER    TRANSFERASE                             27-AUG-08   2K7V              
TITLE     DELETIONS IN A SURFACE LOOP DIVERT THE FOLDING OF A PROTEIN           
TITLE    2 DOMAIN INTO A METASTABLE DIMERIC FORM                                
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: DIHYDROLIPOYLLYSINE-RESIDUE ACETYLTRANSFERASE              
COMPND   3 COMPONENT OF PYRUVATE DEHYDROGENASE COMPLEX;                         
COMPND   4 CHAIN: A, B;                                                         
COMPND   5 FRAGMENT: UNP RESIDUES 206-293;                                      
COMPND   6 SYNONYM: E2, DIHYDROLIPOAMIDE ACETYLTRANSFERASE COMPONENT            
COMPND   7 OF PYRUVATE DEHYDROGENASE COMPLEX;                                   
COMPND   8 EC: 2.3.1.12;                                                        
COMPND   9 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: ESCHERICHIA COLI;                               
SOURCE   3 ORGANISM_TAXID: 83333;                                               
SOURCE   4 STRAIN: K12;                                                         
SOURCE   5 GENE: ACEF;                                                          
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE   8 EXPRESSION_SYSTEM_VECTOR: PET11C                                     
KEYWDS    MISFOLDED DIMER, ACYLTRANSFERASE, GLYCOLYSIS, LIPOYL,                 
KEYWDS   2 TRANSFERASE                                                          
EXPDTA    SOLUTION NMR                                                          
NUMMDL    20                                                                    
AUTHOR    K.M.STOTT,A.M.YUSOF,R.N.PERHAM,D.D.JONES                              
REVDAT   2   22-SEP-09 2K7V    1       JRNL                                     
REVDAT   1   15-SEP-09 2K7V    0                                                
JRNL        AUTH   K.M.STOTT,A.M.YUSOF,R.N.PERHAM,D.D.JONES                     
JRNL        TITL   A SURFACE LOOP DIRECTS CONFORMATIONAL SWITCHING OF           
JRNL        TITL 2 A LIPOYL DOMAIN BETWEEN A FOLDED AND A NOVEL                 
JRNL        TITL 3 MISFOLDED STRUCTURE.                                         
JRNL        REF    STRUCTURE                     V.  17  1117 2009              
JRNL        REFN                   ISSN 0969-2126                               
JRNL        PMID   19679089                                                     
JRNL        DOI    10.1016/J.STR.2009.07.001                                    
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : ARIA                                                 
REMARK   3   AUTHORS     : LINGE, O'DONOGHUE AND NILGES                         
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 2K7V COMPLIES WITH FORMAT V. 3.20, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 03-SEP-08.                  
REMARK 100 THE RCSB ID CODE IS RCSB100786.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 298                                
REMARK 210  PH                             : 6.0                                
REMARK 210  IONIC STRENGTH                 : 20                                 
REMARK 210  PRESSURE                       : AMBIENT                            
REMARK 210  SAMPLE CONTENTS                : 1 MM [U-15N] E2PLIPD4, 20 MM       
REMARK 210                                   SODIUM PHOSPHATE, 40 UM TSP,       
REMARK 210                                   90% H2O/10% D2O                    
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 2D 1H-15N HSQC; 2D 1H-1H           
REMARK 210                                   NOESY; 3D 1H-15N NOESY; 3D 1H-     
REMARK 210                                   15N TOCSY; 2D 1H-1H TOCSY; 2D      
REMARK 210                                   DQF-COSY                           
REMARK 210  SPECTROMETER FIELD STRENGTH    : 500 MHZ                            
REMARK 210  SPECTROMETER MODEL             : DRX                                
REMARK 210  SPECTROMETER MANUFACTURER      : BRUKER                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : ARIA                               
REMARK 210   METHOD USED                   : DGSA-DISTANCE GEOMETRY             
REMARK 210                                   SIMULATED ANNEALING                
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 100                                
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 20                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : STRUCTURES WITH THE LOWEST         
REMARK 210                                   ENERGY                             
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: NULL                                                         
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 LYS A   3      117.52     64.44                                   
REMARK 500  1 VAL A  15      -18.56   -150.99                                   
REMARK 500  1 MET A  21     -171.15     58.17                                   
REMARK 500  1 ALA A  30      149.92   -170.21                                   
REMARK 500  1 GLU A  31     -160.94     57.63                                   
REMARK 500  1 SER A  33      -75.19   -156.49                                   
REMARK 500  1 LEU A  34      162.96     61.32                                   
REMARK 500  1 ILE A  35      101.50     58.99                                   
REMARK 500  1 VAL A  37      -79.84   -107.44                                   
REMARK 500  1 GLU A  38      -71.56   -139.29                                   
REMARK 500  1 LYS A  41      -84.69    -55.47                                   
REMARK 500  1 ALA A  42      -98.59   -164.19                                   
REMARK 500  1 ILE A  71      -50.35   -133.30                                   
REMARK 500  1 ALA A  83      -55.85   -140.40                                   
REMARK 500  1 ALA A  84      -50.28   -167.29                                   
REMARK 500  1 ALA A  86      -41.04   -148.17                                   
REMARK 500  1 ILE B  10      -24.54   -166.38                                   
REMARK 500  1 VAL B  17       95.13     60.95                                   
REMARK 500  1 GLU B  19       15.43     59.87                                   
REMARK 500  1 MET B  21      -40.89   -165.36                                   
REMARK 500  1 LYS B  23       94.26     56.54                                   
REMARK 500  1 VAL B  24      -78.45    -96.08                                   
REMARK 500  1 VAL B  28      -72.46    -81.65                                   
REMARK 500  1 ALA B  29      173.54     60.98                                   
REMARK 500  1 ILE B  35       92.94     61.03                                   
REMARK 500  1 THR B  36      -91.67     52.82                                   
REMARK 500  1 LYS B  41       89.94   -170.12                                   
REMARK 500  1 ALA B  42      -92.43   -113.61                                   
REMARK 500  1 ILE B  71      -50.52   -133.37                                   
REMARK 500  1 ALA B  83      -83.77     62.77                                   
REMARK 500  1 ALA B  84       85.91     56.22                                   
REMARK 500  1 GLN B  88      -33.20   -145.02                                   
REMARK 500  2 VAL A   2      -27.70   -164.80                                   
REMARK 500  2 GLU A   4      -55.96   -126.66                                   
REMARK 500  2 GLU A  19       19.14     59.45                                   
REMARK 500  2 VAL A  20     -179.58    -69.58                                   
REMARK 500  2 VAL A  22      100.18     60.88                                   
REMARK 500  2 LYS A  27      -26.88   -141.51                                   
REMARK 500  2 ALA A  30      -63.70   -103.37                                   
REMARK 500  2 GLN A  32      -76.73    -79.77                                   
REMARK 500  2 SER A  33       95.39     56.96                                   
REMARK 500  2 ILE A  71      -44.70   -132.30                                   
REMARK 500  2 ALA A  86     -171.45     58.61                                   
REMARK 500  2 LYS A  87     -171.77     58.24                                   
REMARK 500  2 VAL B   2      -29.27   -140.70                                   
REMARK 500  2 LYS B   3      -72.94    -77.61                                   
REMARK 500  2 ILE B  10      112.08     62.70                                   
REMARK 500  2 VAL B  15      133.37     65.66                                   
REMARK 500  2 GLU B  16       -0.65   -161.27                                   
REMARK 500  2 THR B  18      -49.56   -171.85                                   
REMARK 500  2 GLU B  19      -62.74   -172.72                                   
REMARK 500  2 LYS B  23      -25.49   -167.37                                   
REMARK 500  2 ALA B  30       95.12     59.22                                   
REMARK 500  2 GLU B  38       99.96     60.04                                   
REMARK 500  2 LYS B  41       33.91   -159.44                                   
REMARK 500  2 ILE B  71      -43.52   -132.12                                   
REMARK 500  2 ALA B  82      -68.95   -161.04                                   
REMARK 500  2 ALA B  83       16.01     59.04                                   
REMARK 500  2 ALA B  86      -61.06   -159.98                                   
REMARK 500  2 LYS B  87       98.13     59.22                                   
REMARK 500  3 GLU A   4       98.12     59.67                                   
REMARK 500  3 VAL A   5      -10.65   -163.12                                   
REMARK 500  3 LYS A  23       94.54     59.07                                   
REMARK 500  3 VAL A  28      -82.32     63.45                                   
REMARK 500  3 GLN A  32      -61.90   -132.54                                   
REMARK 500  3 LYS A  41      -78.68     63.93                                   
REMARK 500  3 ILE A  71      -55.99   -126.20                                   
REMARK 500  3 ALA A  82      -51.59   -148.19                                   
REMARK 500  3 ASP B   9       -0.36   -160.09                                   
REMARK 500  3 VAL B  20      -31.26   -136.78                                   
REMARK 500  3 VAL B  22      114.68     64.12                                   
REMARK 500  3 VAL B  24      -65.19    -92.23                                   
REMARK 500  3 VAL B  28      -70.44   -119.81                                   
REMARK 500  3 ALA B  29       90.06     53.76                                   
REMARK 500  3 ALA B  30      -65.90   -159.11                                   
REMARK 500  3 SER B  33      -49.49   -146.19                                   
REMARK 500  3 LYS B  41      -28.49   -164.46                                   
REMARK 500  3 LYS B  64      170.07    -56.92                                   
REMARK 500  3 ILE B  71      -55.34   -127.01                                   
REMARK 500  3 ALA B  82     -171.25     57.33                                   
REMARK 500  3 LYS B  87       84.91     35.59                                   
REMARK 500  4 LYS A   3     -170.30     59.56                                   
REMARK 500  4 ASN A   6     -177.61     59.44                                   
REMARK 500  4 ILE A  10      -85.67     61.83                                   
REMARK 500  4 VAL A  15       96.84     57.07                                   
REMARK 500  4 GLU A  16      -69.64   -153.91                                   
REMARK 500  4 LYS A  27      -75.88    -85.65                                   
REMARK 500  4 VAL A  28      -37.85   -144.51                                   
REMARK 500  4 ALA A  29      119.47   -168.63                                   
REMARK 500  4 ALA A  30      -54.26   -149.12                                   
REMARK 500  4 ALA A  42      -87.24    -91.53                                   
REMARK 500  4 ALA A  82      -81.05     64.12                                   
REMARK 500  4 ALA A  84       84.95     54.41                                   
REMARK 500  4 ALA A  86      -50.32   -145.39                                   
REMARK 500  4 LYS B   3      -46.84   -142.47                                   
REMARK 500  4 ASP B   9      -54.75   -157.97                                   
REMARK 500  4 VAL B  20      116.17     63.44                                   
REMARK 500  4 LYS B  23      -25.05   -159.58                                   
REMARK 500  4 ALA B  29       13.83   -140.20                                   
REMARK 500  4 ALA B  30      112.35     62.51                                   
REMARK 500  4 GLU B  31      -74.54    -56.12                                   
REMARK 500  4 SER B  33      102.41   -169.23                                   
REMARK 500  4 LEU B  34       97.25     56.92                                   
REMARK 500  4 ILE B  35      -24.74   -140.16                                   
REMARK 500  4 LYS B  41       85.22   -162.96                                   
REMARK 500  4 ALA B  42      -79.77   -171.24                                   
REMARK 500  4 PRO B  81      171.84    -55.46                                   
REMARK 500  4 ALA B  82       97.04     59.80                                   
REMARK 500  4 ALA B  83      -24.33   -159.89                                   
REMARK 500  4 LYS B  87      117.09     63.44                                   
REMARK 500  4 GLN B  88      145.65     65.38                                   
REMARK 500  5 ILE A  10      127.15     65.62                                   
REMARK 500  5 THR A  18      -31.49   -131.47                                   
REMARK 500  5 GLU A  19      120.37     63.05                                   
REMARK 500  5 VAL A  24     -159.12     41.10                                   
REMARK 500  5 GLU A  31     -161.00     43.09                                   
REMARK 500  5 THR A  36       -4.82   -156.90                                   
REMARK 500  5 GLU A  38      -52.84   -147.13                                   
REMARK 500  5 LYS A  41       89.67     57.73                                   
REMARK 500  5 LYS A  64      169.66    -47.82                                   
REMARK 500  5 ALA A  83      -88.27     62.59                                   
REMARK 500  5 ASN B   6      100.18     60.05                                   
REMARK 500  5 ASP B   9     -177.28     58.38                                   
REMARK 500  5 LYS B  23      -87.72     57.55                                   
REMARK 500  5 LYS B  27      106.21     62.36                                   
REMARK 500  5 ALA B  30       18.23     59.10                                   
REMARK 500  5 LYS B  55      -49.12   -131.77                                   
REMARK 500  5 LYS B  58      -38.43   -130.39                                   
REMARK 500  5 LYS B  64      174.62    -55.93                                   
REMARK 500  5 ALA B  83       17.30     58.29                                   
REMARK 500  5 GLN B  88      -34.54   -156.47                                   
REMARK 500  6 VAL A   2      -27.97   -167.46                                   
REMARK 500  6 ASP A   9      -34.78   -138.21                                   
REMARK 500  6 GLU A  16      -58.68   -151.67                                   
REMARK 500  6 THR A  18      -51.93   -139.01                                   
REMARK 500  6 GLU A  19       18.82     58.64                                   
REMARK 500  6 VAL A  20      100.41     59.42                                   
REMARK 500  6 VAL A  24      -32.36   -144.32                                   
REMARK 500  6 VAL A  28      137.72     65.29                                   
REMARK 500  6 ALA A  29      -54.75   -130.10                                   
REMARK 500  6 ALA A  30       97.45     58.19                                   
REMARK 500  6 SER A  33      104.48     61.49                                   
REMARK 500  6 GLU A  38     -176.73     60.39                                   
REMARK 500  6 ILE A  71      -51.23   -136.11                                   
REMARK 500  6 ALA A  83      100.30     60.24                                   
REMARK 500  6 LYS A  87      -41.91   -130.40                                   
REMARK 500  6 GLU B   4      113.60     62.68                                   
REMARK 500  6 ASP B   9      -27.92   -148.81                                   
REMARK 500  6 VAL B  22      -28.81   -164.52                                   
REMARK 500  6 VAL B  28      -30.47   -168.25                                   
REMARK 500  6 ALA B  29      174.42     61.33                                   
REMARK 500  6 ALA B  30        4.55   -165.47                                   
REMARK 500  6 ILE B  35       97.63     59.18                                   
REMARK 500  6 LYS B  41       91.64   -176.95                                   
REMARK 500  6 ILE B  71      -51.47   -136.40                                   
REMARK 500  6 ALA B  86      108.85     61.99                                   
REMARK 500  7 ASP A   9      -76.57   -112.82                                   
REMARK 500  7 ILE A  10      132.99     64.87                                   
REMARK 500  7 MET A  21     -176.85     60.81                                   
REMARK 500  7 VAL A  24       98.72     58.85                                   
REMARK 500  7 ALA A  30       17.86     59.93                                   
REMARK 500  7 GLN A  32      106.83     61.47                                   
REMARK 500  7 LYS A  41       93.86     57.91                                   
REMARK 500  7 LYS A  64      150.99    -48.92                                   
REMARK 500  7 ALA A  82      104.59   -167.56                                   
REMARK 500  7 ALA A  86     -176.05     59.71                                   
REMARK 500  7 GLN A  88      111.28     63.43                                   
REMARK 500  7 ILE B  10      -25.47   -141.76                                   
REMARK 500  7 MET B  21      102.15     61.54                                   
REMARK 500  7 LYS B  23      -77.63    -59.04                                   
REMARK 500  7 VAL B  24      -75.61   -129.52                                   
REMARK 500  7 ILE B  35      -79.19     64.58                                   
REMARK 500  7 GLU B  38      172.24     62.55                                   
REMARK 500  7 ALA B  42      -17.52     69.93                                   
REMARK 500  7 LYS B  87      -71.66   -135.29                                   
REMARK 500  7 GLN B  88      -54.89   -140.19                                   
REMARK 500  8 ASN A   6       93.96     57.43                                   
REMARK 500  8 ASP A   9      -66.02    -95.16                                   
REMARK 500  8 VAL A  15      101.25     61.04                                   
REMARK 500  8 VAL A  20      -33.04   -165.62                                   
REMARK 500  8 LYS A  41       94.53     58.85                                   
REMARK 500  8 ALA A  82      177.31     59.42                                   
REMARK 500  8 ALA A  83       16.91     58.94                                   
REMARK 500  8 ALA A  86      -71.19   -131.69                                   
REMARK 500  8 LYS A  87      -31.09   -163.29                                   
REMARK 500  8 ASN B   6      -63.23   -102.92                                   
REMARK 500  8 ASP B   9      -20.75   -165.24                                   
REMARK 500  8 ILE B  10      106.34     61.83                                   
REMARK 500  8 MET B  21      105.34     60.79                                   
REMARK 500  8 ALA B  29       13.90     58.99                                   
REMARK 500  8 ILE B  35       98.27     59.42                                   
REMARK 500  8 LYS B  41      -71.49   -139.29                                   
REMARK 500  8 ALA B  82      -78.49   -162.36                                   
REMARK 500  8 ALA B  83       89.60     56.14                                   
REMARK 500  8 ALA B  84      -51.41   -169.87                                   
REMARK 500  8 ALA B  86     -178.75     58.48                                   
REMARK 500  9 LYS A   3      103.80     61.04                                   
REMARK 500  9 ASN A   6     -170.27     60.85                                   
REMARK 500  9 GLU A  16      -67.79   -156.27                                   
REMARK 500  9 GLU A  19      -55.37   -128.06                                   
REMARK 500  9 VAL A  20      102.37     61.03                                   
REMARK 500  9 LYS A  27      -40.32   -164.25                                   
REMARK 500  9 ALA A  29      -81.93     62.13                                   
REMARK 500  9 ALA A  30     -177.94     61.06                                   
REMARK 500  9 LEU A  34      -75.12    -96.35                                   
REMARK 500  9 THR A  36     -168.19     51.17                                   
REMARK 500  9 LYS A  64      153.07    -48.15                                   
REMARK 500  9 ILE A  71      -52.13   -122.34                                   
REMARK 500  9 PRO A  81     -172.04    -66.53                                   
REMARK 500  9 GLN A  88      101.33     61.03                                   
REMARK 500  9 VAL B   2      101.61     61.80                                   
REMARK 500  9 VAL B  20      144.31     65.06                                   
REMARK 500  9 LYS B  23      -66.55   -169.92                                   
REMARK 500  9 VAL B  24      -24.74   -154.47                                   
REMARK 500  9 LYS B  27       -8.05   -141.30                                   
REMARK 500  9 ALA B  29      -66.94   -166.45                                   
REMARK 500  9 GLU B  31      -27.47   -161.65                                   
REMARK 500  9 LEU B  34       99.26     60.49                                   
REMARK 500  9 THR B  36      -51.67   -141.73                                   
REMARK 500  9 LYS B  41       89.43   -169.65                                   
REMARK 500  9 ILE B  71      -52.41   -122.78                                   
REMARK 500  9 ALA B  82      -17.90   -157.49                                   
REMARK 500  9 GLN B  88     -177.57     59.73                                   
REMARK 500 10 LYS A   3       96.28     58.97                                   
REMARK 500 10 ASN A   6      -24.83   -153.50                                   
REMARK 500 10 ASP A   9      -34.06   -137.20                                   
REMARK 500 10 THR A  18      -58.84   -147.27                                   
REMARK 500 10 MET A  21       98.61     58.35                                   
REMARK 500 10 ALA A  29      -15.83   -149.98                                   
REMARK 500 10 ALA A  30      -85.51     60.52                                   
REMARK 500 10 ALA A  42      -87.07   -129.08                                   
REMARK 500 10 ALA A  86       11.98   -159.69                                   
REMARK 500 10 GLN A  88     -169.38     57.75                                   
REMARK 500 10 ASN B   6       98.40     58.99                                   
REMARK 500 10 LYS B  23       96.23     59.87                                   
REMARK 500 10 LYS B  27      106.01     59.76                                   
REMARK 500 10 GLU B  31       -7.13   -146.06                                   
REMARK 500 10 LEU B  34      102.78     60.89                                   
REMARK 500 10 VAL B  37       97.69     59.00                                   
REMARK 500 10 ALA B  42      -79.51     65.41                                   
REMARK 500 10 ALA B  86      -68.27   -170.20                                   
REMARK 500 10 GLN B  88      -11.85   -146.11                                   
REMARK 500 11 LYS A   3       99.29     60.04                                   
REMARK 500 11 VAL A   5      144.41     64.15                                   
REMARK 500 11 VAL A  15      -72.20   -120.97                                   
REMARK 500 11 VAL A  17      -70.54    -67.91                                   
REMARK 500 11 VAL A  20     -162.39     42.92                                   
REMARK 500 11 MET A  21       -1.13   -156.40                                   
REMARK 500 11 LYS A  23      -72.64    -71.53                                   
REMARK 500 11 VAL A  28      -64.89   -127.65                                   
REMARK 500 11 ALA A  30      -60.54   -156.78                                   
REMARK 500 11 GLU A  31     -172.78     59.13                                   
REMARK 500 11 GLN A  32       15.95     58.58                                   
REMARK 500 11 LEU A  34      -78.73   -102.54                                   
REMARK 500 11 ILE A  35      -29.68   -170.31                                   
REMARK 500 11 LYS A  41     -169.87    -74.33                                   
REMARK 500 11 LYS A  55      -39.33   -143.39                                   
REMARK 500 11 ALA A  84       78.01     56.06                                   
REMARK 500 11 LYS A  87      115.61     64.07                                   
REMARK 500 11 GLU B   4       94.12     57.75                                   
REMARK 500 11 VAL B   5       86.98     34.34                                   
REMARK 500 11 ASP B   9      -17.74   -165.25                                   
REMARK 500 11 VAL B  17      -72.07    -73.84                                   
REMARK 500 11 THR B  18      103.78     61.73                                   
REMARK 500 11 MET B  21      -42.64   -162.50                                   
REMARK 500 11 GLN B  32      -32.72   -130.61                                   
REMARK 500 11 THR B  36      100.29     59.99                                   
REMARK 500 11 VAL B  37      -36.38   -134.92                                   
REMARK 500 11 GLU B  38      102.06   -166.88                                   
REMARK 500 11 ALA B  42      119.81   -174.03                                   
REMARK 500 11 LYS B  55      -38.56   -144.63                                   
REMARK 500 11 ALA B  82      -55.59   -143.45                                   
REMARK 500 11 ALA B  83     -179.27     60.70                                   
REMARK 500 11 GLN B  88      -74.98   -108.29                                   
REMARK 500 12 VAL A   2      -84.26     61.93                                   
REMARK 500 12 LYS A   3      106.28     59.62                                   
REMARK 500 12 VAL A  15       26.49     44.70                                   
REMARK 500 12 GLU A  16      -64.56   -100.64                                   
REMARK 500 12 VAL A  22      -76.78   -101.22                                   
REMARK 500 12 LYS A  23       95.31     56.79                                   
REMARK 500 12 VAL A  28      -30.61   -135.22                                   
REMARK 500 12 ALA A  30      107.37     61.23                                   
REMARK 500 12 LEU A  34      101.33     61.28                                   
REMARK 500 12 GLU A  38      -67.15   -134.24                                   
REMARK 500 12 LYS A  64      173.28    -54.37                                   
REMARK 500 12 ILE A  71      -33.29   -136.29                                   
REMARK 500 12 ASN B   6      107.38     61.43                                   
REMARK 500 12 ASP B   9      100.86     58.54                                   
REMARK 500 12 MET B  21      -64.25   -104.73                                   
REMARK 500 12 ILE B  35      -78.20    -60.53                                   
REMARK 500 12 THR B  36      -64.38   -159.46                                   
REMARK 500 12 ALA B  42      121.09   -170.08                                   
REMARK 500 12 LYS B  64      175.73    -57.26                                   
REMARK 500 12 ILE B  71      -34.70   -134.50                                   
REMARK 500 12 LYS B  87      148.50     65.00                                   
REMARK 500 12 GLN B  88      104.51     62.30                                   
REMARK 500 13 ASN A   6     -178.82     58.65                                   
REMARK 500 13 GLU A  19      105.22     60.86                                   
REMARK 500 13 MET A  21      -50.40   -151.22                                   
REMARK 500 13 VAL A  22      123.16     64.82                                   
REMARK 500 13 LYS A  23      101.50     60.85                                   
REMARK 500 13 LYS A  27      -80.48   -107.66                                   
REMARK 500 13 VAL A  28      -10.61   -141.01                                   
REMARK 500 13 GLU A  38      113.88   -162.28                                   
REMARK 500 13 ALA A  42      -85.33    -75.02                                   
REMARK 500 13 GLU A  56      119.03   -163.84                                   
REMARK 500 13 LYS A  64      151.18    -48.97                                   
REMARK 500 13 ALA A  84      -51.97   -167.83                                   
REMARK 500 13 ALA A  86      -18.60   -165.40                                   
REMARK 500 13 VAL B   2       93.03     58.99                                   
REMARK 500 13 MET B  21      -76.60   -105.39                                   
REMARK 500 13 VAL B  22     -158.92     39.84                                   
REMARK 500 13 LYS B  27      -70.35   -138.98                                   
REMARK 500 13 SER B  33       85.01     48.99                                   
REMARK 500 13 LYS B  41       76.71     54.03                                   
REMARK 500 13 ALA B  42      -79.77    -89.02                                   
REMARK 500 13 GLU B  56      119.78   -162.87                                   
REMARK 500 13 ALA B  82      -57.99   -164.21                                   
REMARK 500 13 GLN B  88      -27.26   -149.81                                   
REMARK 500 14 VAL A  15       -7.33   -140.84                                   
REMARK 500 14 THR A  18      133.13     65.50                                   
REMARK 500 14 VAL A  28      114.83     63.89                                   
REMARK 500 14 ALA A  29      -22.77   -141.97                                   
REMARK 500 14 SER A  33      -96.02     47.42                                   
REMARK 500 14 LEU A  34       -8.05   -170.45                                   
REMARK 500 14 LYS A  41      116.52   -165.83                                   
REMARK 500 14 ALA A  42      -81.64   -147.41                                   
REMARK 500 14 MET A  44      119.56   -161.72                                   
REMARK 500 14 LYS A  64      174.64    -55.90                                   
REMARK 500 14 ALA A  82      -34.97   -153.88                                   
REMARK 500 14 ALA A  83      -68.45   -138.62                                   
REMARK 500 14 ALA A  84       92.27     55.97                                   
REMARK 500 14 ALA A  86      -46.83   -159.35                                   
REMARK 500 14 GLN A  88      118.40     63.96                                   
REMARK 500 14 LYS B   3       99.41     59.44                                   
REMARK 500 14 THR B  18       87.51     48.33                                   
REMARK 500 14 MET B  21       98.40     59.52                                   
REMARK 500 14 VAL B  28     -163.63     42.86                                   
REMARK 500 14 GLN B  32      104.48     59.60                                   
REMARK 500 14 THR B  36       94.05     58.94                                   
REMARK 500 14 GLU B  38      103.37     61.15                                   
REMARK 500 14 ALA B  42      -85.66     62.61                                   
REMARK 500 15 LYS A   3       43.16    -97.76                                   
REMARK 500 15 ASN A   6       -8.73   -165.26                                   
REMARK 500 15 ASP A   9      -49.65   -136.06                                   
REMARK 500 15 VAL A  22      -64.14   -105.82                                   
REMARK 500 15 ALA A  29       87.53     56.25                                   
REMARK 500 15 SER A  33      125.45     64.35                                   
REMARK 500 15 LYS A  41      -58.28   -137.86                                   
REMARK 500 15 ALA A  42      -83.33   -163.60                                   
REMARK 500 15 ALA A  83      -84.52     62.36                                   
REMARK 500 15 ALA A  86      -21.35   -154.50                                   
REMARK 500 15 VAL B   2       93.05     58.24                                   
REMARK 500 15 VAL B   5      109.44     62.14                                   
REMARK 500 15 VAL B  15      -73.96   -110.03                                   
REMARK 500 15 ALA B  29       -3.55   -142.67                                   
REMARK 500 15 THR B  36      -51.26   -134.89                                   
REMARK 500 15 LYS B  41       91.64     61.88                                   
REMARK 500 15 ALA B  42      -77.84    -53.27                                   
REMARK 500 15 GLN B  88      -45.96   -144.14                                   
REMARK 500 16 GLU A   4       79.57     58.62                                   
REMARK 500 16 ILE A  10      104.35     61.57                                   
REMARK 500 16 GLU A  16      -21.32   -146.78                                   
REMARK 500 16 VAL A  22      -32.04   -165.93                                   
REMARK 500 16 LYS A  23      104.30     59.91                                   
REMARK 500 16 ALA A  29      -64.13   -167.73                                   
REMARK 500 16 GLN A  32      102.73     59.55                                   
REMARK 500 16 LYS A  41       28.85   -167.49                                   
REMARK 500 16 GLU A  56      110.18   -163.33                                   
REMARK 500 16 ILE A  71      -55.58   -132.10                                   
REMARK 500 16 ALA A  82       -8.79   -154.20                                   
REMARK 500 16 ALA A  83       12.05   -142.95                                   
REMARK 500 16 ALA A  86       -1.79   -162.03                                   
REMARK 500 16 GLN A  88     -173.06     59.21                                   
REMARK 500 16 GLU B   4     -167.82     59.23                                   
REMARK 500 16 ASN B   6     -175.51   -172.33                                   
REMARK 500 16 VAL B  22      101.04     60.15                                   
REMARK 500 16 VAL B  28      -29.73   -159.50                                   
REMARK 500 16 GLN B  32     -170.87     57.10                                   
REMARK 500 16 ALA B  42      123.43     67.40                                   
REMARK 500 16 GLU B  56      111.74   -162.68                                   
REMARK 500 16 ILE B  71      -54.34   -131.36                                   
REMARK 500 16 ALA B  82      -14.69   -140.00                                   
REMARK 500 16 ALA B  86      -60.82   -102.52                                   
REMARK 500 16 LYS B  87      165.63     63.04                                   
REMARK 500 17 ASP A   9      -14.85   -154.57                                   
REMARK 500 17 ILE A  10      102.08     60.84                                   
REMARK 500 17 THR A  18      -88.78   -148.81                                   
REMARK 500 17 LYS A  23      -66.79   -138.50                                   
REMARK 500 17 VAL A  24      -31.07   -142.56                                   
REMARK 500 17 ILE A  35      102.96     60.88                                   
REMARK 500 17 GLU A  38      -88.47     60.67                                   
REMARK 500 17 ALA A  42      -90.80    -69.55                                   
REMARK 500 17 ALA A  82     -166.27     57.60                                   
REMARK 500 17 ALA A  84       86.27     56.56                                   
REMARK 500 17 ALA A  86      -10.03   -165.74                                   
REMARK 500 17 LYS A  87       98.90   -160.17                                   
REMARK 500 17 GLN A  88      108.53     59.12                                   
REMARK 500 17 VAL B   2       99.56     60.18                                   
REMARK 500 17 ILE B  10      100.76     58.18                                   
REMARK 500 17 VAL B  15      -25.89   -165.74                                   
REMARK 500 17 THR B  18      -38.05   -162.63                                   
REMARK 500 17 GLU B  19       95.89     58.86                                   
REMARK 500 17 MET B  21      -83.50     61.30                                   
REMARK 500 17 LYS B  23      -61.77   -149.25                                   
REMARK 500 17 VAL B  24       92.89     59.57                                   
REMARK 500 17 ALA B  30      -34.86   -154.58                                   
REMARK 500 17 ILE B  35      -65.70    -92.60                                   
REMARK 500 17 LYS B  41      -53.44   -140.25                                   
REMARK 500 17 ALA B  42      -85.31     62.89                                   
REMARK 500 17 GLN B  88       95.00     58.02                                   
REMARK 500 18 VAL A   5      138.87     65.49                                   
REMARK 500 18 ILE A  10       99.62     60.06                                   
REMARK 500 18 GLN A  32      -65.71   -100.42                                   
REMARK 500 18 LYS A  41     -165.76    -78.81                                   
REMARK 500 18 ALA A  42      -64.46    -94.52                                   
REMARK 500 18 ILE A  71      -51.32   -122.76                                   
REMARK 500 18 ALA A  82     -172.69     58.66                                   
REMARK 500 18 ALA A  83       -3.07   -142.48                                   
REMARK 500 18 ALA A  86      -80.95     62.67                                   
REMARK 500 18 LYS A  87     -177.12     61.75                                   
REMARK 500 18 LYS B   3      109.52     62.34                                   
REMARK 500 18 ASN B   6       90.60   -168.24                                   
REMARK 500 18 VAL B  24      -67.95   -127.43                                   
REMARK 500 18 ALA B  29      103.22     62.40                                   
REMARK 500 18 GLN B  32      -67.90   -133.80                                   
REMARK 500 18 SER B  33      117.68     63.01                                   
REMARK 500 18 THR B  36      115.25     63.65                                   
REMARK 500 18 ALA B  42      -69.99    -92.05                                   
REMARK 500 18 ILE B  71      -51.40   -121.48                                   
REMARK 500 18 ALA B  82     -174.22     57.50                                   
REMARK 500 18 ALA B  84       87.11     57.32                                   
REMARK 500 19 ASN A   6        1.66   -159.49                                   
REMARK 500 19 GLU A  16      121.57     64.64                                   
REMARK 500 19 GLU A  19       89.90     57.76                                   
REMARK 500 19 LYS A  23      -70.87    -83.91                                   
REMARK 500 19 VAL A  24      146.72     64.62                                   
REMARK 500 19 GLN A  32       31.69   -167.27                                   
REMARK 500 19 SER A  33       90.77     50.68                                   
REMARK 500 19 VAL A  37      -74.44    -99.41                                   
REMARK 500 19 GLU A  38      -62.50   -136.00                                   
REMARK 500 19 LYS A  41      112.95     64.32                                   
REMARK 500 19 ALA A  83      -68.79   -143.54                                   
REMARK 500 19 ALA A  84       99.60   -165.91                                   
REMARK 500 19 GLN A  88      -34.31   -134.38                                   
REMARK 500 19 VAL B   2      -31.62   -153.69                                   
REMARK 500 19 GLU B   4      -36.42   -147.19                                   
REMARK 500 19 THR B  18      -77.36   -143.75                                   
REMARK 500 19 GLU B  19      -38.77   -171.50                                   
REMARK 500 19 ALA B  29     -172.93     58.07                                   
REMARK 500 19 ALA B  30     -169.52     57.05                                   
REMARK 500 19 GLU B  31      110.81   -164.52                                   
REMARK 500 19 SER B  33       19.31   -171.73                                   
REMARK 500 19 THR B  36       -9.58   -147.66                                   
REMARK 500 19 LYS B  41       19.75   -161.64                                   
REMARK 500 20 GLU A   4     -174.59     58.70                                   
REMARK 500 20 ASN A   6       13.69   -153.90                                   
REMARK 500 20 ASP A   9     -168.92     57.55                                   
REMARK 500 20 GLU A  16       -1.27   -155.52                                   
REMARK 500 20 VAL A  17      102.42     61.45                                   
REMARK 500 20 ALA A  29       98.69     59.91                                   
REMARK 500 20 SER A  33      -46.65   -141.27                                   
REMARK 500 20 THR A  36       99.92     59.25                                   
REMARK 500 20 GLU A  38      -56.43   -157.68                                   
REMARK 500 20 LYS A  58      -41.89     74.26                                   
REMARK 500 20 LYS A  64      158.50    -48.01                                   
REMARK 500 20 ILE A  71      -41.07   -134.12                                   
REMARK 500 20 ALA A  84       82.02   -155.32                                   
REMARK 500 20 VAL B   2      -57.54   -132.47                                   
REMARK 500 20 ASP B   9      164.05     63.59                                   
REMARK 500 20 GLU B  19       99.50     59.56                                   
REMARK 500 20 LYS B  23      -43.35   -170.72                                   
REMARK 500 20 LYS B  27      -82.92     63.13                                   
REMARK 500 20 VAL B  28      106.32     61.64                                   
REMARK 500 20 ALA B  30     -169.02     57.14                                   
REMARK 500 20 GLN B  32      -90.72     57.31                                   
REMARK 500 20 SER B  33      -83.22     61.08                                   
REMARK 500 20 LEU B  34     -164.67   -124.30                                   
REMARK 500 20 GLU B  38      -52.95   -137.53                                   
REMARK 500 20 LYS B  41       83.68   -161.01                                   
REMARK 500 20 ALA B  42      121.60   -175.43                                   
REMARK 500 20 LYS B  58      -41.60     74.02                                   
REMARK 500 20 ILE B  71      -39.75   -134.52                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 15931   RELATED DB: BMRB                                 
DBREF  2K7V A    2    89  UNP    P06959   ODP2_ECOLI     206    293             
DBREF  2K7V B    2    89  UNP    P06959   ODP2_ECOLI     206    293             
SEQADV 2K7V MET A    1  UNP  P06959              EXPRESSION TAG                 
SEQADV 2K7V     A       UNP  P06959    GLY   215 DELETION                       
SEQADV 2K7V     A       UNP  P06959    GLY   216 DELETION                       
SEQADV 2K7V     A       UNP  P06959    ASP   217 DELETION                       
SEQADV 2K7V     A       UNP  P06959    GLU   218 DELETION                       
SEQADV 2K7V MET B    1  UNP  P06959              EXPRESSION TAG                 
SEQADV 2K7V     B       UNP  P06959    GLY   215 DELETION                       
SEQADV 2K7V     B       UNP  P06959    GLY   216 DELETION                       
SEQADV 2K7V     B       UNP  P06959    ASP   217 DELETION                       
SEQADV 2K7V     B       UNP  P06959    GLU   218 DELETION                       
SEQRES   1 A   85  MET VAL LYS GLU VAL ASN VAL PRO ASP ILE VAL GLU VAL          
SEQRES   2 A   85  THR GLU VAL MET VAL LYS VAL GLY ASP LYS VAL ALA ALA          
SEQRES   3 A   85  GLU GLN SER LEU ILE THR VAL GLU GLY ASP LYS ALA SER          
SEQRES   4 A   85  MET GLU VAL PRO ALA PRO PHE ALA GLY VAL VAL LYS GLU          
SEQRES   5 A   85  LEU LYS VAL ASN VAL GLY ASP LYS VAL LYS THR GLY SER          
SEQRES   6 A   85  LEU ILE MET ILE PHE GLU VAL GLU GLY ALA ALA PRO ALA          
SEQRES   7 A   85  ALA ALA PRO ALA LYS GLN GLU                                  
SEQRES   1 B   85  MET VAL LYS GLU VAL ASN VAL PRO ASP ILE VAL GLU VAL          
SEQRES   2 B   85  THR GLU VAL MET VAL LYS VAL GLY ASP LYS VAL ALA ALA          
SEQRES   3 B   85  GLU GLN SER LEU ILE THR VAL GLU GLY ASP LYS ALA SER          
SEQRES   4 B   85  MET GLU VAL PRO ALA PRO PHE ALA GLY VAL VAL LYS GLU          
SEQRES   5 B   85  LEU LYS VAL ASN VAL GLY ASP LYS VAL LYS THR GLY SER          
SEQRES   6 B   85  LEU ILE MET ILE PHE GLU VAL GLU GLY ALA ALA PRO ALA          
SEQRES   7 B   85  ALA ALA PRO ALA LYS GLN GLU                                  
SHEET    1   A 3 SER A  43  PRO A  47  0                                        
SHEET    2   A 3 LEU B  70  GLU B  75 -1  O  PHE B  74   N  MET A  44           
SHEET    3   A 3 VAL B  54  LEU B  57 -1  O  LYS B  55   N  ILE B  73           
SHEET    1   B 3 GLU A  56  LEU A  57  0                                        
SHEET    2   B 3 LEU A  70  GLU A  75 -1  O  ILE A  73   N  GLU A  56           
SHEET    3   B 3 SER B  43  PRO B  47 -1  O  VAL B  46   N  MET A  72           
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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