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Database: PDB
Entry: 2RMK
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Original site: 2RMK 
HEADER    MEMBRANE PROTEIN/TRANSFERASE            25-OCT-07   2RMK              
TITLE     RAC1/PRK1 COMPLEX                                                     
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: RAS-RELATED C3 BOTULINUM TOXIN SUBSTRATE 1;                
COMPND   3 CHAIN: A;                                                            
COMPND   4 SYNONYM: P21-RAC1, RAS-LIKE PROTEIN TC25, CELL MIGRATION-            
COMPND   5 INDUCING GENE 5 PROTEIN;                                             
COMPND   6 ENGINEERED: YES;                                                     
COMPND   7 MUTATION: YES;                                                       
COMPND   8 MOL_ID: 2;                                                           
COMPND   9 MOLECULE: SERINE/THREONINE-PROTEIN KINASE N1;                        
COMPND  10 CHAIN: B;                                                            
COMPND  11 FRAGMENT: HR1B DOMAIN, REM 2, UNP RESIDUES 122-199;                  
COMPND  12 SYNONYM: PRK1_HR1B, PROTEIN KINASE C-LIKE 1, PROTEIN-                
COMPND  13 KINASE C-RELATED KINASE 1, PROTEIN KINASE C-LIKE PKN,                
COMPND  14 SERINE-THREONINE PROTEIN KINASE N, PROTEIN KINASE PKN-               
COMPND  15 ALPHA;                                                               
COMPND  16 EC: 2.7.11.13;                                                       
COMPND  17 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: RAC1;                                                          
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE   8 EXPRESSION_SYSTEM_VECTOR_TYPE: VECTOR;                               
SOURCE   9 EXPRESSION_SYSTEM_VECTOR: PGEX-2T;                                   
SOURCE  10 MOL_ID: 2;                                                           
SOURCE  11 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  12 ORGANISM_COMMON: HUMAN;                                              
SOURCE  13 ORGANISM_TAXID: 9606;                                                
SOURCE  14 GENE: PKN1, PKN, PRK1, PRKCL1;                                       
SOURCE  15 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  16 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE  17 EXPRESSION_SYSTEM_VECTOR_TYPE: VECTOR;                               
SOURCE  18 EXPRESSION_SYSTEM_VECTOR: PGEX-3X                                    
KEYWDS    G PROTEIN, EFFECTOR, ADP-RIBOSYLATION, ALTERNATIVE SPLICING,          
KEYWDS   2 GTP-BINDING, LIPOPROTEIN, MEMBRANE, METHYLATION, NUCLEOTIDE-         
KEYWDS   3 BINDING, POLYMORPHISM, PRENYLATION, ATP-BINDING, CYTOPLASM,          
KEYWDS   4 KINASE, PHOSPHORYLATION, SERINE/THREONINE-PROTEIN KINASE,            
KEYWDS   5 TRANSFERASE, MEMBRANE PROTEIN/TRANSFERASE COMPLEX                    
EXPDTA    SOLUTION NMR                                                          
NUMMDL    25                                                                    
AUTHOR    R.MODHA,L.J.CAMPBELL,D.NIETLISPACH,H.R.BUHECHA,D.OWEN,                
AUTHOR   2 H.R.MOTT                                                             
REVDAT   3   24-FEB-09 2RMK    1       VERSN                                    
REVDAT   2   29-JAN-08 2RMK    1       JRNL                                     
REVDAT   1   13-NOV-07 2RMK    0                                                
JRNL        AUTH   R.MODHA,L.J.CAMPBELL,D.NIETLISPACH,H.R.BUHECHA,              
JRNL        AUTH 2 D.OWEN,H.R.MOTT                                              
JRNL        TITL   THE RAC1 POLYBASIC REGION IS REQUIRED FOR                    
JRNL        TITL 2 INTERACTION WITH ITS EFFECTOR PRK1                           
JRNL        REF    J.BIOL.CHEM.                  V. 283  1492 2008              
JRNL        REFN                   ISSN 0021-9258                               
JRNL        PMID   18006505                                                     
JRNL        DOI    10.1074/JBC.M706760200                                       
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : CNS 1.1                                              
REMARK   3   AUTHORS     : BRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE-              
REMARK   3                 KUNSTLEVE,JIANG,KUSZEWSKI,NILGES, PANNU,READ,        
REMARK   3                 RICE,SIMONSON,WARREN                                 
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 2RMK COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 31-OCT-07.                  
REMARK 100 THE RCSB ID CODE IS RCSB150036.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 298                                
REMARK 210  PH                             : 7.4                                
REMARK 210  IONIC STRENGTH                 : 0.225                              
REMARK 210  PRESSURE                       : AMBIENT                            
REMARK 210  SAMPLE CONTENTS                : 0.6MM [U-13C; U-15N; U-2H]         
REMARK 210                                   RAC1; 0.6MM PRK1 HR1B; 0.7MM       
REMARK 210                                   PHOSPHOMETHYLPHOSPHONIC ACID       
REMARK 210                                   GUANYLATE ESTER; 90% H2O, 10%      
REMARK 210                                   D2O; 0.6MM [U-100% 13C; U-100%     
REMARK 210                                   15N] RAC1; 0.6MM PRK1 HR1B;        
REMARK 210                                   0.7MM PHOSPHOMETHYLPHOSPHONIC      
REMARK 210                                   ACID GUANYLATE ESTER; 90% H2O,     
REMARK 210                                   10% D2O; 0.6MM [U-100% 15N]        
REMARK 210                                   RAC1; 0.6MM PRK1 HR1B; 0.7MM       
REMARK 210                                   PHOSPHOMETHYLPHOSPHONIC ACID       
REMARK 210                                   GUANYLATE ESTER; 90% H2O, 10%      
REMARK 210                                   D2O; 0.6MM [U-100% 13C; U-100%     
REMARK 210                                   15N] RAC1; 0.6MM PRK1 RAC1;        
REMARK 210                                   0.7MM PHOSPHOMETHYLPHOSPHONIC      
REMARK 210                                   ACID GUANYLATE ESTER; 90% H2O,     
REMARK 210                                   10% D2O; 0.6MM [U-100% 15N]        
REMARK 210                                   HR1B; 0.6MM PRK1 RAC1; 0.7MM       
REMARK 210                                   PHOSPHOMETHYLPHOSPHONIC ACID       
REMARK 210                                   GUANYLATE ESTER; 90% H2O, 10%      
REMARK 210                                   D2O                                
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 3D 1H-15N NOESY, 3D 1H-13C         
REMARK 210                                   NOESY, 3D 13C-EDITED, 13C-         
REMARK 210                                   SEPARATED NOESY                    
REMARK 210  SPECTROMETER FIELD STRENGTH    : 600 MHZ, 800 MHZ                   
REMARK 210  SPECTROMETER MODEL             : DRX                                
REMARK 210  SPECTROMETER MANUFACTURER      : BRUKER                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : AZARA 2.7, ANSIG, ARIA 1.2,        
REMARK 210                                   CNS 1.1                            
REMARK 210   METHOD USED                   : SIMULATED ANNEALING                
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 100                                
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 25                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : STRUCTURES WITH THE LOWEST         
REMARK 210                                   ENERGY                             
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: NULL                                                         
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 GLN A   2       97.45    -56.96                                   
REMARK 500  1 ALA A  27      118.87   -170.74                                   
REMARK 500  1 GLU A  31      127.23     64.41                                   
REMARK 500  1 VAL A  36      -41.51   -131.74                                   
REMARK 500  1 ASP A  38      -45.33   -145.27                                   
REMARK 500  1 TYR A  40       91.54   -171.23                                   
REMARK 500  1 THR A  75      116.13    -36.13                                   
REMARK 500  1 LYS A 116       70.08     56.64                                   
REMARK 500  1 LYS A 153      148.48   -171.79                                   
REMARK 500  1 CYS A 178      -76.13    -80.43                                   
REMARK 500  1 PRO A 179      177.78    -48.44                                   
REMARK 500  1 PRO A 181     -171.67    -61.63                                   
REMARK 500  1 VAL A 182     -101.68   -120.98                                   
REMARK 500  1 LYS A 183       96.87     52.03                                   
REMARK 500  1 LYS A 186      118.96   -161.28                                   
REMARK 500  1 PRO B 121      -98.58    -75.46                                   
REMARK 500  1 THR B 123       38.68    -79.46                                   
REMARK 500  1 LYS B 158      -91.96     58.67                                   
REMARK 500  2 GLN A   2       97.87    -55.24                                   
REMARK 500  2 ALA A  27      120.69   -172.25                                   
REMARK 500  2 THR A  35      -11.85   -171.15                                   
REMARK 500  2 VAL A  36      -88.04    -74.44                                   
REMARK 500  2 ASP A  38      -71.79   -172.70                                   
REMARK 500  2 ASN A  39      106.03   -170.54                                   
REMARK 500  2 ALA A  59     -174.08     61.43                                   
REMARK 500  2 ASP A  65      -84.47     54.82                                   
REMARK 500  2 LEU A  67       35.83    -83.65                                   
REMARK 500  2 THR A  75      123.22    -36.26                                   
REMARK 500  2 THR A 115      175.38    -55.54                                   
REMARK 500  2 LYS A 153      149.01   -171.51                                   
REMARK 500  2 ARG A 163       49.35    -83.40                                   
REMARK 500  2 PRO A 179      178.01    -58.13                                   
REMARK 500  2 LYS A 183      -85.13   -141.85                                   
REMARK 500  2 LYS A 184       95.77     56.74                                   
REMARK 500  2 CYS A 189        7.92   -158.73                                   
REMARK 500  2 PRO B 121     -101.89    -68.31                                   
REMARK 500  2 ASN B 154      172.92     59.98                                   
REMARK 500  2 THR B 157      122.62     64.66                                   
REMARK 500  2 GLU B 194       23.74   -157.86                                   
REMARK 500  2 ASN B 195      -51.09   -126.70                                   
REMARK 500  2 GLN B 196      -70.70    -55.47                                   
REMARK 500  2 ALA B 197       15.58     58.06                                   
REMARK 500  3 GLN A   2       97.18    -63.75                                   
REMARK 500  3 ALA A  27      110.80   -168.53                                   
REMARK 500  3 PRO A  34      173.16    -58.07                                   
REMARK 500  3 ASN A  39       89.35     57.84                                   
REMARK 500  3 THR A  58     -162.72   -102.10                                   
REMARK 500  3 ALA A  59     -164.50     64.37                                   
REMARK 500  3 ASP A  65      -80.54     63.93                                   
REMARK 500  3 ARG A  68      -51.81   -176.21                                   
REMARK 500  3 TYR A  72      -56.45    -29.68                                   
REMARK 500  3 THR A  75      113.09    -34.13                                   
REMARK 500  3 CYS A 105       96.14    179.27                                   
REMARK 500  3 LYS A 153      144.51   -171.40                                   
REMARK 500  3 ARG A 163      -74.16    -39.97                                   
REMARK 500  3 PRO A 179      175.73    -56.91                                   
REMARK 500  3 PRO A 181     -168.51    -57.56                                   
REMARK 500  3 VAL A 182      -76.68   -130.01                                   
REMARK 500  3 LYS A 183     -175.94     56.86                                   
REMARK 500  3 ARG A 185      -63.81   -148.37                                   
REMARK 500  3 PRO B 121     -145.02    -73.81                                   
REMARK 500  3 SER B 156       99.70    -59.76                                   
REMARK 500  3 GLU B 194      -26.34   -144.83                                   
REMARK 500  4 GLN A   2      100.05    -57.78                                   
REMARK 500  4 ALA A  27      116.60   -168.68                                   
REMARK 500  4 PRO A  34     -163.77    -68.42                                   
REMARK 500  4 PHE A  37     -171.53   -174.14                                   
REMARK 500  4 ASP A  38     -167.73   -114.96                                   
REMARK 500  4 ASN A  39       90.38     59.43                                   
REMARK 500  4 TYR A  40       54.30   -146.37                                   
REMARK 500  4 ALA A  59     -165.82   -173.81                                   
REMARK 500  4 ARG A  68      -50.55    171.30                                   
REMARK 500  4 PRO A  69       49.81    -79.82                                   
REMARK 500  4 TYR A  72      -56.75    -26.23                                   
REMARK 500  4 THR A  75      113.45    -27.96                                   
REMARK 500  4 LYS A 116       71.86     56.31                                   
REMARK 500  4 LYS A 153      145.33   -171.78                                   
REMARK 500  4 CYS A 178      -72.91    -89.10                                   
REMARK 500  4 PRO A 179      173.27    -40.90                                   
REMARK 500  4 PRO A 181     -169.65    -58.79                                   
REMARK 500  4 VAL A 182      -89.24   -119.47                                   
REMARK 500  4 LYS A 183      152.99     61.03                                   
REMARK 500  4 LYS A 186       94.46   -163.63                                   
REMARK 500  4 PRO B 121     -148.76    -70.36                                   
REMARK 500  4 SER B 153      -77.77    -63.40                                   
REMARK 500  4 LYS B 158      -70.44    -91.66                                   
REMARK 500  4 GLU B 194       13.05   -168.35                                   
REMARK 500  5 GLN A   2       97.36    -55.08                                   
REMARK 500  5 VAL A  14      140.45    171.00                                   
REMARK 500  5 ALA A  27      119.40   -173.10                                   
REMARK 500  5 GLU A  31      -83.12    -97.02                                   
REMARK 500  5 TYR A  32       -6.53   -177.52                                   
REMARK 500  5 PHE A  37       89.51   -174.96                                   
REMARK 500  5 ASP A  38     -167.32     56.47                                   
REMARK 500  5 ALA A  59      178.47     60.70                                   
REMARK 500  5 ASP A  65      -89.88     57.41                                   
REMARK 500  5 LEU A  67       90.67    -59.32                                   
REMARK 500  5 PRO A  69      104.65    -44.05                                   
REMARK 500  5 THR A  75      118.75    -39.04                                   
REMARK 500  5 CYS A 105      100.24    175.49                                   
REMARK 500  5 LYS A 116       71.23     55.00                                   
REMARK 500  5 LYS A 153      147.39   -171.55                                   
REMARK 500  5 PRO A 179     -177.76    -62.19                                   
REMARK 500  5 PRO A 181     -171.62    -56.19                                   
REMARK 500  5 VAL A 182      -92.39   -121.90                                   
REMARK 500  5 LYS A 183     -140.86     56.31                                   
REMARK 500  5 LYS A 184      110.50     66.24                                   
REMARK 500  5 PRO B 121      -78.70    -74.42                                   
REMARK 500  5 THR B 123     -168.32   -161.65                                   
REMARK 500  5 ASP B 159       92.69     59.13                                   
REMARK 500  5 GLU B 194        4.30   -152.52                                   
REMARK 500  5 ASN B 195      -80.08   -141.18                                   
REMARK 500  5 GLN B 196       91.71     53.14                                   
REMARK 500  6 GLN A   2       97.20    -57.46                                   
REMARK 500  6 ASP A  38      113.31     63.17                                   
REMARK 500  6 TYR A  64       39.43    -83.57                                   
REMARK 500  6 THR A  75      118.76    -38.87                                   
REMARK 500  6 LYS A 116       70.99     58.30                                   
REMARK 500  6 LYS A 153      147.97   -171.42                                   
REMARK 500  6 CYS A 178      -74.07    -81.49                                   
REMARK 500  6 PRO A 179      170.87    -48.09                                   
REMARK 500  6 PRO A 181     -170.46    -57.07                                   
REMARK 500  6 VAL A 182     -104.48   -120.87                                   
REMARK 500  6 LYS A 183       97.87     53.40                                   
REMARK 500  6 CYS A 189      -71.98   -154.18                                   
REMARK 500  6 PRO B 121      -96.17    -76.28                                   
REMARK 500  6 ASP B 159       94.97    -66.37                                   
REMARK 500  6 GLU B 194       -4.66   -162.73                                   
REMARK 500  7 GLN A   2       97.80    -60.20                                   
REMARK 500  7 VAL A  14      144.55     65.73                                   
REMARK 500  7 ALA A  27      104.12   -162.06                                   
REMARK 500  7 PRO A  29     -166.28    -75.82                                   
REMARK 500  7 THR A  35       92.64     60.25                                   
REMARK 500  7 ASN A  39      100.07   -164.45                                   
REMARK 500  7 ALA A  59     -167.19   -169.05                                   
REMARK 500  7 TYR A  64       31.48    -85.48                                   
REMARK 500  7 ARG A  68      -50.92   -178.64                                   
REMARK 500  7 PRO A  69       45.55    -90.81                                   
REMARK 500  7 THR A  75      134.64    -38.45                                   
REMARK 500  7 LYS A 153      147.41   -171.15                                   
REMARK 500  7 ARG A 163      -72.34    -30.94                                   
REMARK 500  7 PRO A 179      174.91    -57.04                                   
REMARK 500  7 PRO A 181     -172.98    -56.80                                   
REMARK 500  7 VAL A 182      -98.35   -119.61                                   
REMARK 500  7 LYS A 183      159.00     60.11                                   
REMARK 500  7 LYS A 186      101.67   -172.04                                   
REMARK 500  7 PRO B 121      -97.74    -74.15                                   
REMARK 500  7 THR B 123       38.29    -81.63                                   
REMARK 500  7 ASN B 154      158.28     64.14                                   
REMARK 500  7 LYS B 158      -70.81    -92.28                                   
REMARK 500  7 GLU B 194      -83.03     53.06                                   
REMARK 500  7 ASN B 195      -74.31    -97.30                                   
REMARK 500  7 GLN B 196       88.95     57.85                                   
REMARK 500  8 ALA A  27      122.92   -173.65                                   
REMARK 500  8 GLU A  31     -179.58     59.65                                   
REMARK 500  8 TYR A  32      -59.74   -149.96                                   
REMARK 500  8 ILE A  33      137.08     62.15                                   
REMARK 500  8 ASP A  38      118.98   -163.62                                   
REMARK 500  8 ASN A  39       89.46   -166.67                                   
REMARK 500  8 THR A  58     -164.75   -120.71                                   
REMARK 500  8 ALA A  59     -171.48     63.10                                   
REMARK 500  8 ASP A  65      -79.79     61.65                                   
REMARK 500  8 ARG A  68      -50.89    178.98                                   
REMARK 500  8 TYR A  72      -57.04    -28.72                                   
REMARK 500  8 LYS A 116       71.98     58.48                                   
REMARK 500  8 LYS A 153      147.52   -172.02                                   
REMARK 500  8 PRO A 179     -179.23    -57.45                                   
REMARK 500  8 PRO A 181     -173.80    -59.26                                   
REMARK 500  8 VAL A 182     -119.41   -120.26                                   
REMARK 500  8 LYS A 183       98.29     54.15                                   
REMARK 500  8 ARG A 185      146.42     65.39                                   
REMARK 500  8 PRO B 121     -138.32    -72.06                                   
REMARK 500  8 ASP B 159       96.43    -63.59                                   
REMARK 500  8 GLU B 194      -81.00     61.93                                   
REMARK 500  8 GLN B 196       95.23     63.06                                   
REMARK 500  9 ILE A  33      109.30    -50.99                                   
REMARK 500  9 ASP A  65      -79.85     62.61                                   
REMARK 500  9 LEU A  67       91.65    -69.41                                   
REMARK 500  9 PRO A  69      106.22    -48.63                                   
REMARK 500  9 CYS A 105       95.54   -177.96                                   
REMARK 500  9 LYS A 153      148.11   -172.10                                   
REMARK 500  9 CYS A 178      -74.60    -80.02                                   
REMARK 500  9 PRO A 179      177.08    -46.88                                   
REMARK 500  9 PRO A 181     -172.66    -58.58                                   
REMARK 500  9 VAL A 182      -96.61   -121.24                                   
REMARK 500  9 LYS A 183       97.44     49.57                                   
REMARK 500  9 LYS A 188     -168.75   -124.91                                   
REMARK 500  9 PRO B 121      -93.51    -79.32                                   
REMARK 500  9 THR B 123       38.24    -80.83                                   
REMARK 500  9 ASN B 154     -176.46     60.31                                   
REMARK 500  9 LYS B 158       75.46   -105.72                                   
REMARK 500  9 GLU B 194       20.06   -162.28                                   
REMARK 500  9 ASN B 195      -48.20   -130.77                                   
REMARK 500 10 GLN A   2       97.88    -61.86                                   
REMARK 500 10 ALA A  27      107.71   -171.39                                   
REMARK 500 10 TYR A  64       30.38    -82.43                                   
REMARK 500 10 LEU A  67      -86.41    -63.47                                   
REMARK 500 10 ARG A  68      159.76     58.67                                   
REMARK 500 10 THR A  75      123.12    -38.90                                   
REMARK 500 10 THR A 115      171.92    -58.69                                   
REMARK 500 10 LYS A 116       71.61     54.71                                   
REMARK 500 10 LYS A 153      148.76   -172.25                                   
REMARK 500 10 CYS A 178      -75.07    -81.17                                   
REMARK 500 10 PRO A 179      173.59    -47.71                                   
REMARK 500 10 PRO A 181     -179.12    -60.32                                   
REMARK 500 10 LYS A 183      135.60   -177.87                                   
REMARK 500 10 LYS A 186      -85.77   -155.06                                   
REMARK 500 10 ARG A 187     -169.58     54.22                                   
REMARK 500 10 LEU A 190      103.84     61.63                                   
REMARK 500 10 PRO B 121     -152.66    -72.49                                   
REMARK 500 10 THR B 123      -77.54    -52.57                                   
REMARK 500 10 GLU B 194      -25.64   -158.84                                   
REMARK 500 11 GLN A   2       97.12    -59.51                                   
REMARK 500 11 VAL A  14      151.68     68.42                                   
REMARK 500 11 ALA A  27      113.74   -169.28                                   
REMARK 500 11 GLU A  31      177.14     55.15                                   
REMARK 500 11 ASP A  38      133.93     64.80                                   
REMARK 500 11 TYR A  64       37.79    -80.94                                   
REMARK 500 11 THR A  75       91.45     15.82                                   
REMARK 500 11 THR A 115      171.78    -58.42                                   
REMARK 500 11 LYS A 153      148.29   -171.44                                   
REMARK 500 11 PRO A 179      173.72    -53.62                                   
REMARK 500 11 PRO A 181     -170.61    -57.32                                   
REMARK 500 11 VAL A 182      -87.51   -121.81                                   
REMARK 500 11 LYS A 183     -146.72     52.31                                   
REMARK 500 11 LYS A 184      105.21     67.42                                   
REMARK 500 11 LEU A 190       99.01     59.83                                   
REMARK 500 11 PRO B 121     -149.63    -65.77                                   
REMARK 500 11 SER B 153      -81.89    -62.73                                   
REMARK 500 11 ASP B 159       88.81    -68.93                                   
REMARK 500 11 GLU B 194       -0.26   -164.28                                   
REMARK 500 12 GLN A   2       98.00    -58.89                                   
REMARK 500 12 ALA A  27      111.92   -169.52                                   
REMARK 500 12 PHE A  37     -175.81   -175.22                                   
REMARK 500 12 ASN A  39       85.55     58.18                                   
REMARK 500 12 ASP A  65      -82.99     61.91                                   
REMARK 500 12 ARG A  68      -52.83   -172.45                                   
REMARK 500 12 PRO A  69       49.40    -81.26                                   
REMARK 500 12 TYR A  72      -57.83    -29.18                                   
REMARK 500 12 LYS A 116       73.45     57.40                                   
REMARK 500 12 LYS A 153      148.69   -171.39                                   
REMARK 500 12 CYS A 178      -74.31    -78.42                                   
REMARK 500 12 PRO A 179      173.40    -50.64                                   
REMARK 500 12 PRO A 181     -178.48    -56.62                                   
REMARK 500 12 VAL A 182      -88.42   -119.25                                   
REMARK 500 12 LYS A 183       96.01     46.78                                   
REMARK 500 12 LYS A 188      -62.18   -141.01                                   
REMARK 500 12 PRO B 121     -144.07    -75.68                                   
REMARK 500 12 LYS B 158      -12.43   -152.40                                   
REMARK 500 12 GLU B 194      -21.97   -156.26                                   
REMARK 500 13 GLN A   2       97.51    -61.44                                   
REMARK 500 13 ALA A  27      108.94   -166.81                                   
REMARK 500 13 PRO A  29     -172.03    -62.50                                   
REMARK 500 13 GLU A  31      153.22     64.23                                   
REMARK 500 13 TYR A  32       -1.84   -149.19                                   
REMARK 500 13 ASN A  39       90.14     58.85                                   
REMARK 500 13 THR A  58     -166.76   -119.69                                   
REMARK 500 13 ALA A  59     -172.08     59.92                                   
REMARK 500 13 TYR A  64       42.09    -84.29                                   
REMARK 500 13 ARG A  68      -55.99   -175.45                                   
REMARK 500 13 PRO A  69       46.17    -88.16                                   
REMARK 500 13 THR A  75      122.02    -38.07                                   
REMARK 500 13 LYS A 153      146.61   -172.06                                   
REMARK 500 13 CYS A 178      -74.78    -79.39                                   
REMARK 500 13 PRO A 179      174.33    -49.58                                   
REMARK 500 13 PRO A 181     -176.00    -57.17                                   
REMARK 500 13 VAL A 182      -96.57   -117.37                                   
REMARK 500 13 LYS A 183      145.49     61.60                                   
REMARK 500 13 LYS A 186      -72.88   -153.55                                   
REMARK 500 13 ARG A 187      175.79     62.22                                   
REMARK 500 13 PRO B 121     -148.75    -71.27                                   
REMARK 500 13 GLU B 194      -79.34     67.62                                   
REMARK 500 13 ASN B 195      -81.28   -120.77                                   
REMARK 500 14 ALA A  27      118.74   -169.77                                   
REMARK 500 14 TYR A  32     -103.26    -91.23                                   
REMARK 500 14 ILE A  33      115.08     62.56                                   
REMARK 500 14 PRO A  34        3.34    -67.14                                   
REMARK 500 14 THR A  35       91.94     61.48                                   
REMARK 500 14 VAL A  36       93.52   -176.16                                   
REMARK 500 14 PHE A  37      -63.51   -175.95                                   
REMARK 500 14 ARG A  68      -52.04    179.87                                   
REMARK 500 14 PRO A  69       44.41    -81.72                                   
REMARK 500 14 THR A  75      115.27    -38.74                                   
REMARK 500 14 LYS A 116       80.23     61.10                                   
REMARK 500 14 LYS A 153      146.55   -172.30                                   
REMARK 500 14 PRO A 179      172.86    -58.54                                   
REMARK 500 14 PRO A 181     -179.33    -54.01                                   
REMARK 500 14 VAL A 182     -102.89   -112.07                                   
REMARK 500 14 LYS A 183       98.63     46.01                                   
REMARK 500 14 LYS A 186      112.95   -170.17                                   
REMARK 500 14 LEU A 190      101.75     61.31                                   
REMARK 500 14 THR B 123       99.13     60.89                                   
REMARK 500 14 SER B 156       98.50    -65.89                                   
REMARK 500 14 GLU B 194       -1.77   -160.29                                   
REMARK 500 14 ALA B 197      -73.22    -93.58                                   
REMARK 500 15 GLN A   2       96.92    -60.53                                   
REMARK 500 15 VAL A  14      143.82    171.07                                   
REMARK 500 15 ALA A  27      117.16   -169.88                                   
REMARK 500 15 TYR A  32       -2.82   -148.70                                   
REMARK 500 15 THR A  35        1.68   -167.70                                   
REMARK 500 15 ASP A  38       98.52     62.45                                   
REMARK 500 15 THR A  58     -168.36   -120.40                                   
REMARK 500 15 ALA A  59     -166.10     61.58                                   
REMARK 500 15 ASP A  65      -77.28     64.28                                   
REMARK 500 15 ARG A  68      -52.71    179.97                                   
REMARK 500 15 PRO A  69       50.12    -98.94                                   
REMARK 500 15 THR A  75      132.06    -37.59                                   
REMARK 500 15 LYS A 116       70.10     59.74                                   
REMARK 500 15 LYS A 153      147.33   -171.85                                   
REMARK 500 15 CYS A 178      -74.54    -82.67                                   
REMARK 500 15 PRO A 179      176.95    -46.18                                   
REMARK 500 15 PRO A 181     -174.59    -59.71                                   
REMARK 500 15 VAL A 182     -138.41   -115.37                                   
REMARK 500 15 LYS A 183       98.69     56.85                                   
REMARK 500 15 LYS A 184       82.16   -171.85                                   
REMARK 500 15 PRO B 121     -151.23    -75.07                                   
REMARK 500 15 THR B 123      -71.67    -52.49                                   
REMARK 500 15 ASN B 124       17.98     56.38                                   
REMARK 500 15 ASP B 159       96.19    -64.11                                   
REMARK 500 15 GLU B 194      -10.84   -150.30                                   
REMARK 500 15 ALA B 197      156.21     65.15                                   
REMARK 500 16 GLN A   2       95.59    -61.63                                   
REMARK 500 16 TYR A  32       11.07   -163.07                                   
REMARK 500 16 THR A  35        1.08   -166.15                                   
REMARK 500 16 ASN A  39       28.87   -168.88                                   
REMARK 500 16 THR A  75      126.05    -34.71                                   
REMARK 500 16 VAL A  85        6.42    -69.63                                   
REMARK 500 16 LYS A 153      147.21   -172.15                                   
REMARK 500 16 CYS A 178      -74.10    -77.47                                   
REMARK 500 16 PRO A 179      174.11    -49.62                                   
REMARK 500 16 PRO A 181      174.89    -55.59                                   
REMARK 500 16 LYS A 184      108.99   -168.74                                   
REMARK 500 16 LYS A 186      103.78   -166.84                                   
REMARK 500 16 LEU A 190      -87.41     59.07                                   
REMARK 500 16 PRO B 121     -153.99    -71.07                                   
REMARK 500 16 THR B 123      -72.10    -53.50                                   
REMARK 500 16 ASN B 124       16.03     58.69                                   
REMARK 500 16 GLU B 194      -75.90     66.53                                   
REMARK 500 16 GLN B 196      121.04   -170.94                                   
REMARK 500 17 GLN A   2       96.04    -56.96                                   
REMARK 500 17 ALA A  27      113.77   -169.58                                   
REMARK 500 17 PHE A  37      -63.24   -177.77                                   
REMARK 500 17 ARG A  68      -51.84    178.78                                   
REMARK 500 17 TYR A  72      -58.68    -27.03                                   
REMARK 500 17 LYS A 116       70.35     59.67                                   
REMARK 500 17 LYS A 153      148.49   -171.49                                   
REMARK 500 17 CYS A 178      -75.67    -79.90                                   
REMARK 500 17 PRO A 179      176.97    -48.32                                   
REMARK 500 17 PRO A 181     -176.52    -57.75                                   
REMARK 500 17 VAL A 182      -89.42   -118.28                                   
REMARK 500 17 LYS A 183      156.64     62.47                                   
REMARK 500 17 ARG A 185      101.87   -161.43                                   
REMARK 500 17 LYS A 186      119.97   -171.08                                   
REMARK 500 17 PRO B 121     -149.65    -74.94                                   
REMARK 500 17 ASN B 154      -88.53     57.59                                   
REMARK 500 17 LYS B 158        9.09   -164.26                                   
REMARK 500 17 GLU B 194      -76.58     68.02                                   
REMARK 500 17 ASN B 195      -81.90   -127.33                                   
REMARK 500 17 GLN B 196       89.77     45.63                                   
REMARK 500 18 GLN A   2       95.71    -66.23                                   
REMARK 500 18 ALA A  27      123.27   -172.43                                   
REMARK 500 18 GLU A  31     -167.60     57.41                                   
REMARK 500 18 PRO A  34     -175.50    -68.96                                   
REMARK 500 18 ASN A  39       27.01   -168.01                                   
REMARK 500 18 ALA A  59      114.04     63.28                                   
REMARK 500 18 ASP A  65      -79.12     63.41                                   
REMARK 500 18 ARG A  68      -53.49    175.38                                   
REMARK 500 18 PRO A  69       45.73    -78.38                                   
REMARK 500 18 TYR A  72      -56.26    -26.51                                   
REMARK 500 18 THR A  75      126.54    -30.46                                   
REMARK 500 18 LYS A 153      149.02   -171.27                                   
REMARK 500 18 PRO A 179      174.18    -58.23                                   
REMARK 500 18 PRO A 181     -176.17    -54.18                                   
REMARK 500 18 VAL A 182      -91.25   -117.86                                   
REMARK 500 18 LYS A 183       88.69     49.52                                   
REMARK 500 18 PRO B 121     -154.47    -70.97                                   
REMARK 500 18 THR B 123      -72.94    -52.56                                   
REMARK 500 18 ASP B 159       99.01    -60.54                                   
REMARK 500 18 GLU B 194      -81.94     60.25                                   
REMARK 500 18 ASN B 195      -72.14   -111.61                                   
REMARK 500 18 GLN B 196       99.60     59.97                                   
REMARK 500 19 GLN A   2       96.32    -62.51                                   
REMARK 500 19 VAL A  14      151.55    169.98                                   
REMARK 500 19 ALA A  27      114.80   -163.78                                   
REMARK 500 19 GLU A  31      116.97     62.62                                   
REMARK 500 19 TYR A  64       35.78    -79.44                                   
REMARK 500 19 TYR A  72      -60.35    -26.75                                   
REMARK 500 19 LYS A 153      146.80   -172.05                                   
REMARK 500 19 PRO A 179      174.56    -53.03                                   
REMARK 500 19 PRO A 181     -169.90    -55.68                                   
REMARK 500 19 VAL A 182      -90.86   -118.79                                   
REMARK 500 19 LYS A 183     -168.92     52.66                                   
REMARK 500 19 ARG A 185      -88.19    -59.29                                   
REMARK 500 19 CYS A 189      -42.59   -142.41                                   
REMARK 500 19 PRO B 121      -94.91    -75.25                                   
REMARK 500 19 ASN B 154       92.57     56.56                                   
REMARK 500 19 SER B 156       95.08     58.09                                   
REMARK 500 19 GLU B 194       -3.03   -159.66                                   
REMARK 500 19 ALA B 197      -64.63   -129.11                                   
REMARK 500 20 GLN A   2       95.88    -59.66                                   
REMARK 500 20 ALA A  27      124.94   -170.13                                   
REMARK 500 20 THR A  35        5.13   -155.32                                   
REMARK 500 20 ASN A  39       88.79     63.60                                   
REMARK 500 20 TYR A  40       62.84   -150.01                                   
REMARK 500 20 ARG A  68      -49.17   -179.11                                   
REMARK 500 20 PRO A  69       33.52    -73.87                                   
REMARK 500 20 TYR A  72      -58.02    -25.69                                   
REMARK 500 20 THR A  75      124.70    -38.74                                   
REMARK 500 20 ASN A 107       87.40   -161.30                                   
REMARK 500 20 LYS A 153      148.51   -172.10                                   
REMARK 500 20 CYS A 178      -74.97    -77.33                                   
REMARK 500 20 PRO A 179      178.29    -49.89                                   
REMARK 500 20 PRO A 181     -173.75    -55.51                                   
REMARK 500 20 VAL A 182      -97.74   -114.10                                   
REMARK 500 20 LYS A 183     -148.36     47.35                                   
REMARK 500 20 LYS A 184       91.64     63.21                                   
REMARK 500 20 ARG A 187     -179.67    -65.64                                   
REMARK 500 20 LEU A 191       94.72    -65.65                                   
REMARK 500 20 PRO B 121     -147.70    -74.60                                   
REMARK 500 20 SER B 153      -74.77    -68.00                                   
REMARK 500 20 LYS B 158      -16.13   -161.07                                   
REMARK 500 20 GLU B 194      -73.52     65.26                                   
REMARK 500 20 ASN B 195      -59.11   -137.03                                   
REMARK 500 20 GLN B 196       92.43     61.47                                   
REMARK 500 21 ALA A  27      105.32   -164.73                                   
REMARK 500 21 THR A  35      -10.37   -162.50                                   
REMARK 500 21 PHE A  37     -167.23   -168.84                                   
REMARK 500 21 ASN A  39       95.05     62.79                                   
REMARK 500 21 TYR A  40       15.25   -151.35                                   
REMARK 500 21 ASP A  65      -77.81     60.82                                   
REMARK 500 21 ARG A  68      -60.96    105.16                                   
REMARK 500 21 TYR A  72      -54.11    -28.91                                   
REMARK 500 21 THR A  75      135.56    -38.44                                   
REMARK 500 21 LYS A 116       70.63     55.09                                   
REMARK 500 21 LYS A 153      146.80   -172.00                                   
REMARK 500 21 CYS A 178      -73.63    -81.54                                   
REMARK 500 21 PRO A 179      172.15    -49.63                                   
REMARK 500 21 PRO A 181      177.45    -54.49                                   
REMARK 500 21 LYS A 184       85.01   -173.74                                   
REMARK 500 21 CYS A 189       86.97     56.33                                   
REMARK 500 21 LEU A 190      141.58   -170.96                                   
REMARK 500 21 GLU B 194      -79.91     61.42                                   
REMARK 500 21 ASN B 195      -70.95   -118.97                                   
REMARK 500 21 GLN B 196       12.74     58.48                                   
REMARK 500 21 ALA B 197       93.56     56.24                                   
REMARK 500 22 GLN A   2       98.25    -59.97                                   
REMARK 500 22 VAL A  14      149.29     64.65                                   
REMARK 500 22 ALA A  27      116.29   -168.87                                   
REMARK 500 22 ASP A  38      107.69   -161.31                                   
REMARK 500 22 ASN A  39      101.13   -175.05                                   
REMARK 500 22 ALA A  59     -171.14     61.54                                   
REMARK 500 22 TYR A  64       37.94    -78.79                                   
REMARK 500 22 ARG A  68      -52.65   -178.33                                   
REMARK 500 22 PRO A  69       48.75    -86.45                                   
REMARK 500 22 TYR A  72      -57.64    -27.86                                   
REMARK 500 22 LYS A 116       73.74     57.42                                   
REMARK 500 22 LYS A 153      146.27   -172.16                                   
REMARK 500 22 CYS A 178      -75.44    -82.60                                   
REMARK 500 22 PRO A 179      173.65    -47.28                                   
REMARK 500 22 PRO A 181     -174.39    -59.26                                   
REMARK 500 22 VAL A 182     -102.58   -118.44                                   
REMARK 500 22 LYS A 183       94.58     48.00                                   
REMARK 500 22 PRO B 121      -95.32    -75.40                                   
REMARK 500 22 THR B 123       33.28    -81.06                                   
REMARK 500 22 LYS B 158      -63.02    -92.41                                   
REMARK 500 22 GLU B 194      -83.24     60.52                                   
REMARK 500 22 ALA B 197       90.20     57.13                                   
REMARK 500 23 GLN A   2      101.39    -57.48                                   
REMARK 500 23 ALA A  27      115.97   -173.40                                   
REMARK 500 23 VAL A  36      105.33    -56.26                                   
REMARK 500 23 PHE A  37      -73.99   -176.94                                   
REMARK 500 23 TYR A  64       39.01   -143.68                                   
REMARK 500 23 ARG A  68      -53.71    174.08                                   
REMARK 500 23 TYR A  72      -57.64    -20.73                                   
REMARK 500 23 THR A  75       99.16    -14.41                                   
REMARK 500 23 ASN A 107       99.30   -166.11                                   
REMARK 500 23 LYS A 153      148.23   -171.74                                   
REMARK 500 23 PRO B 121      -95.90    -72.02                                   
REMARK 500 23 ASN B 124       97.64     59.03                                   
REMARK 500 23 ASN B 154     -172.22     57.11                                   
REMARK 500 23 SER B 156      130.96   -170.27                                   
REMARK 500 23 THR B 157      -21.84   -156.57                                   
REMARK 500 23 ASP B 159       71.27   -115.71                                   
REMARK 500 23 GLU B 194       -1.35   -154.91                                   
REMARK 500 24 VAL A  14      153.14     64.77                                   
REMARK 500 24 ALA A  27      124.84   -170.67                                   
REMARK 500 24 TYR A  32        7.20   -150.16                                   
REMARK 500 24 THR A  35       19.72   -170.92                                   
REMARK 500 24 VAL A  36      -72.47   -115.17                                   
REMARK 500 24 ALA A  59      105.53     60.81                                   
REMARK 500 24 TYR A  64       34.89    -83.22                                   
REMARK 500 24 LEU A  67       30.47    -90.22                                   
REMARK 500 24 THR A  75      116.86    -37.97                                   
REMARK 500 24 CYS A 105       98.32    177.32                                   
REMARK 500 24 LYS A 153      140.96   -170.45                                   
REMARK 500 24 CYS A 178      -72.83    -71.92                                   
REMARK 500 24 PRO A 179      177.62    -55.85                                   
REMARK 500 24 PRO A 181     -179.90    -57.92                                   
REMARK 500 24 VAL A 182      -96.15   -117.94                                   
REMARK 500 24 LYS A 183      113.98     55.27                                   
REMARK 500 24 ARG A 185      120.40   -171.22                                   
REMARK 500 24 LYS A 188      -75.98    -62.47                                   
REMARK 500 24 CYS A 189      -48.75   -161.88                                   
REMARK 500 24 PRO B 121      -91.32    -78.51                                   
REMARK 500 24 ASN B 154       96.26    -65.49                                   
REMARK 500 24 LYS B 158      -74.64   -130.95                                   
REMARK 500 24 GLU B 194       -4.86   -146.18                                   
REMARK 500 25 GLN A   2       95.29    -62.35                                   
REMARK 500 25 VAL A  14       76.69     58.77                                   
REMARK 500 25 ALA A  27      110.07   -170.08                                   
REMARK 500 25 PRO A  34     -178.40    -55.71                                   
REMARK 500 25 ASP A  38      -98.38   -133.32                                   
REMARK 500 25 ASN A  39       92.40     53.95                                   
REMARK 500 25 THR A  58       25.45   -143.45                                   
REMARK 500 25 TYR A  64       53.03   -143.61                                   
REMARK 500 25 ARG A  68      -50.97    177.61                                   
REMARK 500 25 PRO A  69       42.83    -79.87                                   
REMARK 500 25 THR A  75      127.03    -39.40                                   
REMARK 500 25 LYS A 153      147.66   -171.43                                   
REMARK 500 25 CYS A 178      -75.06    -80.39                                   
REMARK 500 25 PRO A 179      172.46    -47.53                                   
REMARK 500 25 LYS A 183       97.15   -177.17                                   
REMARK 500 25 LYS A 184      108.84   -176.53                                   
REMARK 500 25 LYS A 188      107.38    -52.59                                   
REMARK 500 25 PRO B 121     -151.51    -61.69                                   
REMARK 500 25 GLU B 194        5.13   -160.00                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 620                                                                      
REMARK 620 METAL COORDINATION                                                   
REMARK 620  (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;              
REMARK 620  SSEQ=SEQUENCE NUMBER; I=INSERTION CODE):                            
REMARK 620                                                                      
REMARK 620 COORDINATION ANGLES FOR:  M RES CSSEQI METAL                         
REMARK 620                           1  MG A 194  MG                            
REMARK 620 N RES CSSEQI ATOM                                                    
REMARK 620 1 THR A  17   OG1                                                    
REMARK 620 2 THR A  35   OG1  91.6                                              
REMARK 620 3 GCP A 193   O2B  83.1 173.3                                        
REMARK 620 4 GCP A 193   O2G 171.9  94.1  91.6                                  
REMARK 620 5 HOH A 195   O    95.7  86.5  90.0  90.3                            
REMARK 620 6 HOH A 196   O    84.1  93.4  90.1  89.8 179.8                      
REMARK 620 N                    1     2     3     4     5                       
REMARK 800                                                                      
REMARK 800 SITE                                                                 
REMARK 800 SITE_IDENTIFIER: AC1                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE MG A 194                  
REMARK 800 SITE_IDENTIFIER: AC2                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE GCP A 193                 
DBREF  2RMK A    1   192  UNP    P63000   RAC1_HUMAN       1    192             
DBREF  2RMK B  122   199  UNP    Q16512   PKN1_HUMAN     122    199             
SEQADV 2RMK LEU A   61  UNP  P63000    GLN    61 ENGINEERED                     
SEQADV 2RMK GLY B  119  UNP  Q16512              EXPRESSION TAG                 
SEQADV 2RMK ILE B  120  UNP  Q16512              EXPRESSION TAG                 
SEQADV 2RMK PRO B  121  UNP  Q16512              EXPRESSION TAG                 
SEQRES   1 A  192  MET GLN ALA ILE LYS CYS VAL VAL VAL GLY ASP GLY ALA          
SEQRES   2 A  192  VAL GLY LYS THR CYS LEU LEU ILE SER TYR THR THR ASN          
SEQRES   3 A  192  ALA PHE PRO GLY GLU TYR ILE PRO THR VAL PHE ASP ASN          
SEQRES   4 A  192  TYR SER ALA ASN VAL MET VAL ASP GLY LYS PRO VAL ASN          
SEQRES   5 A  192  LEU GLY LEU TRP ASP THR ALA GLY LEU GLU ASP TYR ASP          
SEQRES   6 A  192  ARG LEU ARG PRO LEU SER TYR PRO GLN THR ASP VAL PHE          
SEQRES   7 A  192  LEU ILE CYS PHE SER LEU VAL SER PRO ALA SER PHE GLU          
SEQRES   8 A  192  ASN VAL ARG ALA LYS TRP TYR PRO GLU VAL ARG HIS HIS          
SEQRES   9 A  192  CYS PRO ASN THR PRO ILE ILE LEU VAL GLY THR LYS LEU          
SEQRES  10 A  192  ASP LEU ARG ASP ASP LYS ASP THR ILE GLU LYS LEU LYS          
SEQRES  11 A  192  GLU LYS LYS LEU THR PRO ILE THR TYR PRO GLN GLY LEU          
SEQRES  12 A  192  ALA MET ALA LYS GLU ILE GLY ALA VAL LYS TYR LEU GLU          
SEQRES  13 A  192  CYS SER ALA LEU THR GLN ARG GLY LEU LYS THR VAL PHE          
SEQRES  14 A  192  ASP GLU ALA ILE ARG ALA VAL LEU CYS PRO PRO PRO VAL          
SEQRES  15 A  192  LYS LYS ARG LYS ARG LYS CYS LEU LEU LEU                      
SEQRES   1 B   81  GLY ILE PRO ALA THR ASN LEU SER ARG VAL ALA GLY LEU          
SEQRES   2 B   81  GLU LYS GLN LEU ALA ILE GLU LEU LYS VAL LYS GLN GLY          
SEQRES   3 B   81  ALA GLU ASN MET ILE GLN THR TYR SER ASN GLY SER THR          
SEQRES   4 B   81  LYS ASP ARG LYS LEU LEU LEU THR ALA GLN GLN MET LEU          
SEQRES   5 B   81  GLN ASP SER LYS THR LYS ILE ASP ILE ILE ARG MET GLN          
SEQRES   6 B   81  LEU ARG ARG ALA LEU GLN ALA ASP GLN LEU GLU ASN GLN          
SEQRES   7 B   81  ALA ALA PRO                                                  
HET     MG  A 194       1                                                       
HET    GCP  A 193      46                                                       
HETNAM      MG MAGNESIUM ION                                                    
HETNAM     GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER                     
FORMUL   3   MG    MG 2+                                                        
FORMUL   4  GCP    C11 H18 N5 O13 P3                                            
FORMUL   5  HOH   *2(H2 O)                                                      
HELIX    1   1 GLY A   15  ASN A   26  1                                  12    
HELIX    2   2 GLU A   62  ARG A   66  5                                   5    
HELIX    3   3 LEU A   70  THR A   75  1                                   6    
HELIX    4   4 SER A   86  CYS A  105  1                                  20    
HELIX    5   5 ASP A  122  LYS A  132  1                                  11    
HELIX    6   6 THR A  138  ILE A  149  1                                  12    
HELIX    7   7 GLY A  164  CYS A  178  1                                  15    
HELIX    8   8 THR B  123  ASN B  154  1                                  32    
HELIX    9   9 ASP B  159  LEU B  193  1                                  35    
SHEET    1   A 6 SER A  41  VAL A  46  0                                        
SHEET    2   A 6 LYS A  49  ASP A  57 -1  O  VAL A  51   N  VAL A  44           
SHEET    3   A 6 GLN A   2  GLY A  10  1  N  ILE A   4   O  ASN A  52           
SHEET    4   A 6 VAL A  77  CYS A  81  1  O  CYS A  81   N  VAL A   9           
SHEET    5   A 6 ILE A 111  GLY A 114  1  O  VAL A 113   N  ILE A  80           
SHEET    6   A 6 LYS A 153  GLU A 156  1  O  LEU A 155   N  LEU A 112           
LINK         OG1 THR A  17                MG    MG A 194     1555   1555  2.41  
LINK         OG1 THR A  35                MG    MG A 194     1555   1555  2.44  
LINK        MG    MG A 194                 O2B GCP A 193     1555   1555  2.30  
LINK        MG    MG A 194                 O2G GCP A 193     1555   1555  2.30  
LINK        MG    MG A 194                 O   HOH A 195     1555   1555  2.30  
LINK        MG    MG A 194                 O   HOH A 196     1555   1555  2.30  
SITE     1 AC1  5 THR A  17  THR A  35  GCP A 193  HOH A 195                    
SITE     2 AC1  5 HOH A 196                                                     
SITE     1 AC2 15 VAL A  14  GLY A  15  LYS A  16  THR A  17                    
SITE     2 AC2 15 CYS A  18  PHE A  28  THR A  35  ALA A  59                    
SITE     3 AC2 15 GLY A  60  LYS A 116  ASP A 118  SER A 158                    
SITE     4 AC2 15 ALA A 159  LEU A 160   MG A 194                               
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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