HEADER TRANSFERASE 06-JUN-08 3DDW
TITLE CRYSTAL STRUCTURE OF GLYCOGEN PHOSPHORYLASE COMPLEXED WITH AN
TITLE 2 ANTHRANILIMIDE BASED INHIBITOR GSK055
COMPND MOL_ID: 1;
COMPND 2 MOLECULE: GLYCOGEN PHOSPHORYLASE, LIVER FORM;
COMPND 3 CHAIN: A, B;
COMPND 4 EC: 2.4.1.1;
COMPND 5 ENGINEERED: YES
SOURCE MOL_ID: 1;
SOURCE 2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;
SOURCE 3 ORGANISM_TAXID: 9606;
SOURCE 4 TISSUE: LIVER ISOFORM;
SOURCE 5 GENE: PYGL;
SOURCE 6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
SOURCE 7 EXPRESSION_SYSTEM_TAXID: 562;
SOURCE 8 EXPRESSION_SYSTEM_STRAIN: BL21;
SOURCE 9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;
SOURCE 10 EXPRESSION_SYSTEM_PLASMID: T7 PROMOTER
KEYWDS GLYCOGEN PHOSPHORYLASE, GP, DIABETES, ALLOSTERIC ENZYME, CARBOHYDRATE
KEYWDS 2 METABOLISM, DISEASE MUTATION, GLYCOGEN METABOLISM, GLYCOGEN STORAGE
KEYWDS 3 DISEASE, GLYCOSYLTRANSFERASE, NUCLEOTIDE-BINDING, PHOSPHOPROTEIN,
KEYWDS 4 PYRIDOXAL PHOSPHATE, TRANSFERASE
EXPDTA X-RAY DIFFRACTION
AUTHOR R.T.NOLTE
REVDAT 3 13-JUL-11 3DDW 1 VERSN
REVDAT 2 03-MAR-09 3DDW 1 JRNL
REVDAT 1 27-JAN-09 3DDW 0
JRNL AUTH S.A.THOMSON,P.BANKER,D.M.BICKETT,J.A.BOUCHERON,H.L.CARTER,
JRNL AUTH 2 D.C.CLANCY,J.P.COOPER,S.H.DICKERSON,D.M.GARRIDO,R.T.NOLTE,
JRNL AUTH 3 A.J.PEAT,L.R.SHECKLER,S.M.SPARKS,F.X.TAVARES,L.WANG,
JRNL AUTH 4 T.Y.WANG,J.E.WEIEL
JRNL TITL ANTHRANILIMIDE BASED GLYCOGEN PHOSPHORYLASE INHIBITORS FOR
JRNL TITL 2 THE TREATMENT OF TYPE 2 DIABETES. PART 3: X-RAY
JRNL TITL 3 CRYSTALLOGRAPHIC CHARACTERIZATION, CORE AND UREA
JRNL TITL 4 OPTIMIZATION AND IN VIVO EFFICACY.
JRNL REF BIOORG.MED.CHEM.LETT. V. 19 1177 2009
JRNL REFN ISSN 0960-894X
JRNL PMID 19138846
JRNL DOI 10.1016/J.BMCL.2008.12.085
REMARK 2
REMARK 2 RESOLUTION. 1.90 ANGSTROMS.
REMARK 3
REMARK 3 REFINEMENT.
REMARK 3 PROGRAM : REFMAC 5.2.0019
REMARK 3 AUTHORS : MURSHUDOV,VAGIN,DODSON
REMARK 3
REMARK 3 REFINEMENT TARGET : MAXIMUM LIKELIHOOD
REMARK 3
REMARK 3 DATA USED IN REFINEMENT.
REMARK 3 RESOLUTION RANGE HIGH (ANGSTROMS) : 1.90
REMARK 3 RESOLUTION RANGE LOW (ANGSTROMS) : 38.63
REMARK 3 DATA CUTOFF (SIGMA(F)) : NULL
REMARK 3 COMPLETENESS FOR RANGE (%) : 99.9
REMARK 3 NUMBER OF REFLECTIONS : 163245
REMARK 3
REMARK 3 FIT TO DATA USED IN REFINEMENT.
REMARK 3 CROSS-VALIDATION METHOD : THROUGHOUT
REMARK 3 FREE R VALUE TEST SET SELECTION : RANDOM
REMARK 3 R VALUE (WORKING + TEST SET) : 0.154
REMARK 3 R VALUE (WORKING SET) : 0.153
REMARK 3 FREE R VALUE : 0.186
REMARK 3 FREE R VALUE TEST SET SIZE (%) : 3.000
REMARK 3 FREE R VALUE TEST SET COUNT : 5094
REMARK 3
REMARK 3 FIT IN THE HIGHEST RESOLUTION BIN.
REMARK 3 TOTAL NUMBER OF BINS USED : 20
REMARK 3 BIN RESOLUTION RANGE HIGH (A) : 1.90
REMARK 3 BIN RESOLUTION RANGE LOW (A) : 1.95
REMARK 3 REFLECTION IN BIN (WORKING SET) : 11989
REMARK 3 BIN COMPLETENESS (WORKING+TEST) (%) : 99.73
REMARK 3 BIN R VALUE (WORKING SET) : 0.2070
REMARK 3 BIN FREE R VALUE SET COUNT : 406
REMARK 3 BIN FREE R VALUE : 0.2440
REMARK 3
REMARK 3 NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.
REMARK 3 PROTEIN ATOMS : 13016
REMARK 3 NUCLEIC ACID ATOMS : 0
REMARK 3 HETEROGEN ATOMS : 198
REMARK 3 SOLVENT ATOMS : 1612
REMARK 3
REMARK 3 B VALUES.
REMARK 3 FROM WILSON PLOT (A**2) : 16.30
REMARK 3 MEAN B VALUE (OVERALL, A**2) : 19.61
REMARK 3 OVERALL ANISOTROPIC B VALUE.
REMARK 3 B11 (A**2) : -0.05000
REMARK 3 B22 (A**2) : -0.05000
REMARK 3 B33 (A**2) : 0.08000
REMARK 3 B12 (A**2) : -0.03000
REMARK 3 B13 (A**2) : 0.00000
REMARK 3 B23 (A**2) : 0.00000
REMARK 3
REMARK 3 ESTIMATED OVERALL COORDINATE ERROR.
REMARK 3 ESU BASED ON R VALUE (A): 0.112
REMARK 3 ESU BASED ON FREE R VALUE (A): 0.108
REMARK 3 ESU BASED ON MAXIMUM LIKELIHOOD (A): 0.073
REMARK 3 ESU FOR B VALUES BASED ON MAXIMUM LIKELIHOOD (A**2): 2.425
REMARK 3
REMARK 3 CORRELATION COEFFICIENTS.
REMARK 3 CORRELATION COEFFICIENT FO-FC : 0.966
REMARK 3 CORRELATION COEFFICIENT FO-FC FREE : 0.952
REMARK 3
REMARK 3 RMS DEVIATIONS FROM IDEAL VALUES COUNT RMS WEIGHT
REMARK 3 BOND LENGTHS REFINED ATOMS (A): 13578 ; 0.010 ; 0.022
REMARK 3 BOND LENGTHS OTHERS (A): 9252 ; 0.002 ; 0.020
REMARK 3 BOND ANGLES REFINED ATOMS (DEGREES): 18394 ; 1.246 ; 1.974
REMARK 3 BOND ANGLES OTHERS (DEGREES): 22530 ; 0.921 ; 3.000
REMARK 3 TORSION ANGLES, PERIOD 1 (DEGREES): 1620 ; 5.732 ; 5.000
REMARK 3 TORSION ANGLES, PERIOD 2 (DEGREES): 651 ;35.630 ;24.332
REMARK 3 TORSION ANGLES, PERIOD 3 (DEGREES): 2385 ;12.788 ;15.013
REMARK 3 TORSION ANGLES, PERIOD 4 (DEGREES): 80 ;17.475 ;15.000
REMARK 3 CHIRAL-CENTER RESTRAINTS (A**3): 1997 ; 0.071 ; 0.200
REMARK 3 GENERAL PLANES REFINED ATOMS (A): 14974 ; 0.005 ; 0.020
REMARK 3 GENERAL PLANES OTHERS (A): 2780 ; 0.005 ; 0.020
REMARK 3 NON-BONDED CONTACTS REFINED ATOMS (A): 2884 ; 0.209 ; 0.200
REMARK 3 NON-BONDED CONTACTS OTHERS (A): 9887 ; 0.189 ; 0.200
REMARK 3 NON-BONDED TORSION REFINED ATOMS (A): 6700 ; 0.180 ; 0.200
REMARK 3 NON-BONDED TORSION OTHERS (A): 6618 ; 0.084 ; 0.200
REMARK 3 H-BOND (X...Y) REFINED ATOMS (A): 1324 ; 0.126 ; 0.200
REMARK 3 H-BOND (X...Y) OTHERS (A): NULL ; NULL ; NULL
REMARK 3 POTENTIAL METAL-ION REFINED ATOMS (A): NULL ; NULL ; NULL
REMARK 3 POTENTIAL METAL-ION OTHERS (A): NULL ; NULL ; NULL
REMARK 3 SYMMETRY VDW REFINED ATOMS (A): 25 ; 0.205 ; 0.200
REMARK 3 SYMMETRY VDW OTHERS (A): 48 ; 0.263 ; 0.200
REMARK 3 SYMMETRY H-BOND REFINED ATOMS (A): 39 ; 0.250 ; 0.200
REMARK 3 SYMMETRY H-BOND OTHERS (A): NULL ; NULL ; NULL
REMARK 3 SYMMETRY METAL-ION REFINED ATOMS (A): NULL ; NULL ; NULL
REMARK 3 SYMMETRY METAL-ION OTHERS (A): NULL ; NULL ; NULL
REMARK 3
REMARK 3 ISOTROPIC THERMAL FACTOR RESTRAINTS. COUNT RMS WEIGHT
REMARK 3 MAIN-CHAIN BOND REFINED ATOMS (A**2): 8436 ; 0.788 ; 1.500
REMARK 3 MAIN-CHAIN BOND OTHER ATOMS (A**2): 3263 ; 0.169 ; 1.500
REMARK 3 MAIN-CHAIN ANGLE REFINED ATOMS (A**2): 13034 ; 1.195 ; 2.000
REMARK 3 SIDE-CHAIN BOND REFINED ATOMS (A**2): 6131 ; 1.837 ; 3.000
REMARK 3 SIDE-CHAIN ANGLE REFINED ATOMS (A**2): 5360 ; 2.794 ; 4.500
REMARK 3
REMARK 3 ANISOTROPIC THERMAL FACTOR RESTRAINTS. COUNT RMS WEIGHT
REMARK 3 RIGID-BOND RESTRAINTS (A**2): NULL ; NULL ; NULL
REMARK 3 SPHERICITY; FREE ATOMS (A**2): NULL ; NULL ; NULL
REMARK 3 SPHERICITY; BONDED ATOMS (A**2): NULL ; NULL ; NULL
REMARK 3
REMARK 3 NCS RESTRAINTS STATISTICS
REMARK 3 NUMBER OF DIFFERENT NCS GROUPS : 1
REMARK 3
REMARK 3 NCS GROUP NUMBER : 1
REMARK 3 CHAIN NAMES : A B
REMARK 3 NUMBER OF COMPONENTS NCS GROUP : 1
REMARK 3 COMPONENT C SSSEQI TO C SSSEQI CODE
REMARK 3 1 A 12 A 830 5
REMARK 3 1 B 23 B 830 5
REMARK 3 GROUP CHAIN COUNT RMS WEIGHT
REMARK 3 MEDIUM POSITIONAL 1 A (A): 4658 ; 0.27 ; 0.50
REMARK 3 LOOSE POSITIONAL 1 A (A): 6185 ; 0.45 ; 5.00
REMARK 3 MEDIUM THERMAL 1 A (A**2): 4658 ; 0.68 ; 2.00
REMARK 3 LOOSE THERMAL 1 A (A**2): 6185 ; 0.99 ; 10.00
REMARK 3
REMARK 3 TLS DETAILS
REMARK 3 NUMBER OF TLS GROUPS : NULL
REMARK 3
REMARK 3 BULK SOLVENT MODELLING.
REMARK 3 METHOD USED : BABINET MODEL WITH MASK
REMARK 3 PARAMETERS FOR MASK CALCULATION
REMARK 3 VDW PROBE RADIUS : 1.20
REMARK 3 ION PROBE RADIUS : 0.80
REMARK 3 SHRINKAGE RADIUS : 0.80
REMARK 3
REMARK 3 OTHER REFINEMENT REMARKS: HYDROGENS HAVE BEEN ADDED IN THE RIDING
REMARK 3 POSITIONS
REMARK 4
REMARK 4 3DDW COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 10-JUN-08.
REMARK 100 THE RCSB ID CODE IS RCSB047916.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200 EXPERIMENT TYPE : X-RAY DIFFRACTION
REMARK 200 DATE OF DATA COLLECTION : 25-AUG-05
REMARK 200 TEMPERATURE (KELVIN) : 100
REMARK 200 PH : 6.0
REMARK 200 NUMBER OF CRYSTALS USED : 1
REMARK 200
REMARK 200 SYNCHROTRON (Y/N) : Y
REMARK 200 RADIATION SOURCE : APS
REMARK 200 BEAMLINE : 17-ID
REMARK 200 X-RAY GENERATOR MODEL : NULL
REMARK 200 MONOCHROMATIC OR LAUE (M/L) : M
REMARK 200 WAVELENGTH OR RANGE (A) : 1.00000
REMARK 200 MONOCHROMATOR : SI(111)
REMARK 200 OPTICS : NULL
REMARK 200
REMARK 200 DETECTOR TYPE : CCD
REMARK 200 DETECTOR MANUFACTURER : ADSC QUANTUM 210
REMARK 200 INTENSITY-INTEGRATION SOFTWARE : HKL-2000
REMARK 200 DATA SCALING SOFTWARE : HKL-2000
REMARK 200
REMARK 200 NUMBER OF UNIQUE REFLECTIONS : 168259
REMARK 200 RESOLUTION RANGE HIGH (A) : 1.900
REMARK 200 RESOLUTION RANGE LOW (A) : 50.000
REMARK 200 REJECTION CRITERIA (SIGMA(I)) : -3.000
REMARK 200
REMARK 200 OVERALL.
REMARK 200 COMPLETENESS FOR RANGE (%) : 99.9
REMARK 200 DATA REDUNDANCY : 5.800
REMARK 200 R MERGE (I) : 0.07800
REMARK 200 R SYM (I) : NULL
REMARK 200 <I/SIGMA(I)> FOR THE DATA SET : 21.5800
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200 HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.90
REMARK 200 HIGHEST RESOLUTION SHELL, RANGE LOW (A) : 1.97
REMARK 200 COMPLETENESS FOR SHELL (%) : 99.7
REMARK 200 DATA REDUNDANCY IN SHELL : 5.60
REMARK 200 R MERGE FOR SHELL (I) : 0.53400
REMARK 200 R SYM FOR SHELL (I) : NULL
REMARK 200 <I/SIGMA(I)> FOR SHELL : 2.700
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: AMORE
REMARK 200 STARTING MODEL: PDB ENTRY 1EM6
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS (%): 56.62
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.84
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: 12-25% MPD, 0.1M NA MES BUFFER PH 6.0,
REMARK 280 5MM CAFFEINE, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 290K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 31
REMARK 290
REMARK 290 SYMOP SYMMETRY
REMARK 290 NNNMMM OPERATOR
REMARK 290 1555 X,Y,Z
REMARK 290 2555 -Y,X-Y,Z+1/3
REMARK 290 3555 -X+Y,-X,Z+2/3
REMARK 290
REMARK 290 WHERE NNN -> OPERATOR NUMBER
REMARK 290 MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290 SMTRY1 1 1.000000 0.000000 0.000000 0.00000
REMARK 290 SMTRY2 1 0.000000 1.000000 0.000000 0.00000
REMARK 290 SMTRY3 1 0.000000 0.000000 1.000000 0.00000
REMARK 290 SMTRY1 2 -0.500000 -0.866025 0.000000 0.00000
REMARK 290 SMTRY2 2 0.866025 -0.500000 0.000000 0.00000
REMARK 290 SMTRY3 2 0.000000 0.000000 1.000000 41.20700
REMARK 290 SMTRY1 3 -0.500000 0.866025 0.000000 0.00000
REMARK 290 SMTRY2 3 -0.866025 -0.500000 0.000000 0.00000
REMARK 290 SMTRY3 3 0.000000 0.000000 1.000000 82.41400
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 300 REMARK: THE ACTIVE FORM IS A DIMER, AS SEEN IN THE ASYMMETRIC UNIT
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW. BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC
REMARK 350 SOFTWARE USED: PISA
REMARK 350 TOTAL BURIED SURFACE AREA: 5050 ANGSTROM**2
REMARK 350 SURFACE AREA OF THE COMPLEX: 57590 ANGSTROM**2
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -25.6 KCAL/MOL
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B
REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000
REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000
REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465 M RES C SSSEQI
REMARK 465 GLY A -1
REMARK 465 GLY A 0
REMARK 465 ALA A 1
REMARK 465 LYS A 2
REMARK 465 PRO A 3
REMARK 465 LEU A 4
REMARK 465 THR A 5
REMARK 465 ASP A 6
REMARK 465 GLN A 7
REMARK 465 GLU A 8
REMARK 465 LYS A 9
REMARK 465 ARG A 10
REMARK 465 ARG A 11
REMARK 465 PHE A 252
REMARK 465 ASN A 253
REMARK 465 LEU A 254
REMARK 465 ARG A 255
REMARK 465 ASP A 256
REMARK 465 PHE A 257
REMARK 465 ASN A 258
REMARK 465 VAL A 259
REMARK 465 ASP A 831
REMARK 465 LEU A 832
REMARK 465 LYS A 833
REMARK 465 ILE A 834
REMARK 465 SER A 835
REMARK 465 LEU A 836
REMARK 465 SER A 837
REMARK 465 ASN A 838
REMARK 465 GLU A 839
REMARK 465 SER A 840
REMARK 465 ASN A 841
REMARK 465 LYS A 842
REMARK 465 VAL A 843
REMARK 465 ASN A 844
REMARK 465 GLY A 845
REMARK 465 ASN A 846
REMARK 465 GLY B -1
REMARK 465 GLY B 0
REMARK 465 ALA B 1
REMARK 465 LYS B 2
REMARK 465 PRO B 3
REMARK 465 LEU B 4
REMARK 465 THR B 5
REMARK 465 ASP B 6
REMARK 465 GLN B 7
REMARK 465 GLU B 8
REMARK 465 LYS B 9
REMARK 465 ARG B 10
REMARK 465 ARG B 11
REMARK 465 GLN B 12
REMARK 465 ILE B 13
REMARK 465 SEP B 14
REMARK 465 ILE B 15
REMARK 465 ARG B 16
REMARK 465 GLY B 17
REMARK 465 ILE B 18
REMARK 465 VAL B 19
REMARK 465 GLY B 20
REMARK 465 VAL B 21
REMARK 465 GLU B 22
REMARK 465 PHE B 257
REMARK 465 ASN B 258
REMARK 465 VAL B 259
REMARK 465 SER B 318
REMARK 465 THR B 319
REMARK 465 ARG B 320
REMARK 465 GLY B 321
REMARK 465 ALA B 322
REMARK 465 ASP B 831
REMARK 465 LEU B 832
REMARK 465 LYS B 833
REMARK 465 ILE B 834
REMARK 465 SER B 835
REMARK 465 LEU B 836
REMARK 465 SER B 837
REMARK 465 ASN B 838
REMARK 465 GLU B 839
REMARK 465 SER B 840
REMARK 465 ASN B 841
REMARK 465 LYS B 842
REMARK 465 VAL B 843
REMARK 465 ASN B 844
REMARK 465 GLY B 845
REMARK 465 ASN B 846
REMARK 470
REMARK 470 MISSING ATOM
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;
REMARK 470 I=INSERTION CODE):
REMARK 470 M RES CSSEQI ATOMS
REMARK 470 ILE A 18 CG1 CG2 CD1
REMARK 470 LYS A 420 CG CD CE NZ
REMARK 470 GLU A 434 CG CD OE1 OE2
REMARK 470 LYS A 786 CD CE NZ
REMARK 470 ARG B 255 CG CD NE CZ NH1 NH2
REMARK 470 LYS B 394 CE NZ
REMARK 470 LYS B 420 CG CD CE NZ
REMARK 470 GLU B 434 CG CD OE1 OE2
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT
REMARK 500
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.
REMARK 500
REMARK 500 ATM1 RES C SSEQI ATM2 RES C SSEQI DISTANCE
REMARK 500 O HOH A 1628 O HOH A 1698 2.18
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS
REMARK 500
REMARK 500 THE FOLLOWING ATOMS THAT ARE RELATED BY CRYSTALLOGRAPHIC
REMARK 500 SYMMETRY ARE IN CLOSE CONTACT. AN ATOM LOCATED WITHIN 0.15
REMARK 500 ANGSTROMS OF A SYMMETRY RELATED ATOM IS ASSUMED TO BE ON A
REMARK 500 SPECIAL POSITION AND IS, THEREFORE, LISTED IN REMARK 375
REMARK 500 INSTEAD OF REMARK 500. ATOMS WITH NON-BLANK ALTERNATE
REMARK 500 LOCATION INDICATORS ARE NOT INCLUDED IN THE CALCULATIONS.
REMARK 500
REMARK 500 DISTANCE CUTOFF:
REMARK 500 2.2 ANGSTROMS FOR CONTACTS NOT INVOLVING HYDROGEN ATOMS
REMARK 500 1.6 ANGSTROMS FOR CONTACTS INVOLVING HYDROGEN ATOMS
REMARK 500
REMARK 500 ATM1 RES C SSEQI ATM2 RES C SSEQI SSYMOP DISTANCE
REMARK 500 NZ LYS A 312 OE2 GLU B 505 2435 2.08
REMARK 500 OE2 GLU B 501 NZ LYS A 312 3644 2.09
REMARK 500 O GLY A 321 O HOH B 1713 2435 2.11
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES
REMARK 500
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)
REMARK 500
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996
REMARK 500
REMARK 500 M RES CSSEQI ATM1 ATM2 ATM3
REMARK 500 ARG A 770 NE - CZ - NH1 ANGL. DEV. = 3.1 DEGREES
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500 M RES CSSEQI PSI PHI
REMARK 500 CYS A 78 61.10 32.23
REMARK 500 LEU A 131 37.97 -90.56
REMARK 500 TYR A 203 -132.03 63.35
REMARK 500 PRO A 281 30.96 -87.16
REMARK 500 ASP A 339 -173.43 67.03
REMARK 500 ARG A 489 -74.24 -72.22
REMARK 500 LEU A 492 -62.29 -146.48
REMARK 500 LYS A 568 164.38 170.75
REMARK 500 SER A 674 -59.07 -137.95
REMARK 500 HIS A 768 34.68 -142.98
REMARK 500 ILE A 824 -54.52 -125.43
REMARK 500 LEU B 131 43.02 -92.61
REMARK 500 TYR B 203 -134.34 62.24
REMARK 500 PRO B 281 31.99 -87.08
REMARK 500 ASP B 339 -171.87 68.25
REMARK 500 PRO B 342 30.02 -96.12
REMARK 500 GLU B 434 79.51 -69.30
REMARK 500 LYS B 466 -70.63 -116.44
REMARK 500 ARG B 489 -72.22 -72.73
REMARK 500 LEU B 492 -62.12 -148.87
REMARK 500 LYS B 568 167.90 173.66
REMARK 500 SER B 674 -60.93 -137.60
REMARK 500 SER B 751 60.58 -150.02
REMARK 500 HIS B 768 35.37 -142.29
REMARK 500 ILE B 824 -56.56 -125.12
REMARK 500
REMARK 500 REMARK: NULL
REMARK 525
REMARK 525 SOLVENT
REMARK 525
REMARK 525 THE SOLVENT MOLECULES HAVE CHAIN IDENTIFIERS THAT
REMARK 525 INDICATE THE POLYMER CHAIN WITH WHICH THEY ARE MOST
REMARK 525 CLOSELY ASSOCIATED. THE REMARK LISTS ALL THE SOLVENT
REMARK 525 MOLECULES WHICH ARE MORE THAN 5A AWAY FROM THE
REMARK 525 NEAREST POLYMER CHAIN (M = MODEL NUMBER;
REMARK 525 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE
REMARK 525 NUMBER; I=INSERTION CODE):
REMARK 525
REMARK 525 M RES CSSEQI
REMARK 525 HOH A1208 DISTANCE = 5.04 ANGSTROMS
REMARK 525 HOH A1359 DISTANCE = 5.53 ANGSTROMS
REMARK 525 HOH A1699 DISTANCE = 5.01 ANGSTROMS
REMARK 525 HOH B1891 DISTANCE = 5.04 ANGSTROMS
REMARK 525 HOH B1899 DISTANCE = 5.72 ANGSTROMS
REMARK 525 HOH B1974 DISTANCE = 5.51 ANGSTROMS
REMARK 525 HOH B2022 DISTANCE = 5.86 ANGSTROMS
REMARK 525 HOH B2056 DISTANCE = 5.03 ANGSTROMS
REMARK 525 HOH B2066 DISTANCE = 5.67 ANGSTROMS
REMARK 525 HOH B2123 DISTANCE = 5.01 ANGSTROMS
REMARK 525 HOH B2172 DISTANCE = 6.86 ANGSTROMS
REMARK 600
REMARK 600 HETEROGEN
REMARK 600 THE PLANARITY OF THE ARRANGEMENT OF ATOM C13 WITH RESPECT
REMARK 600 TO THE PHENYL RING C46-C51 IN BOTH MOLECULES OF LIGAND [055]
REMARK 600 IS DISTORTED.
REMARK 800
REMARK 800 SITE
REMARK 800 SITE_IDENTIFIER: AC1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE NBG A 901
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC2
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE NBG B 901
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC3
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE PLP A 902
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC4
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE PLP B 902
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC5
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE CFF A 903
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC6
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE CFF B 903
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC7
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE MES B 904
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC8
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE MES B 905
REMARK 800
REMARK 800 SITE_IDENTIFIER: AC9
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE 055 A 905
REMARK 800
REMARK 800 SITE_IDENTIFIER: BC1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE 055 B 906
REMARK 800
REMARK 800 SITE_IDENTIFIER: BC2
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE MPD B 907
REMARK 800
REMARK 800 SITE_IDENTIFIER: BC3
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE MPD A 906
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: 3DD1 RELATED DB: PDB
REMARK 900 SAME PROTEIN IN COMPLEX WITH RELATED LIGAND
REMARK 900 RELATED ID: 3DDS RELATED DB: PDB
REMARK 900 SAME PROTEIN IN COMPLEX WITH RELATED LIGAND
DBREF 3DDW A 1 846 UNP P06737 PYGL_HUMAN 2 847
DBREF 3DDW B 1 846 UNP P06737 PYGL_HUMAN 2 847
SEQADV 3DDW GLY A -1 UNP P06737 EXPRESSION TAG
SEQADV 3DDW GLY A 0 UNP P06737 EXPRESSION TAG
SEQADV 3DDW GLY B -1 UNP P06737 EXPRESSION TAG
SEQADV 3DDW GLY B 0 UNP P06737 EXPRESSION TAG
SEQRES 1 A 848 GLY GLY ALA LYS PRO LEU THR ASP GLN GLU LYS ARG ARG
SEQRES 2 A 848 GLN ILE SEP ILE ARG GLY ILE VAL GLY VAL GLU ASN VAL
SEQRES 3 A 848 ALA GLU LEU LYS LYS SER PHE ASN ARG HIS LEU HIS PHE
SEQRES 4 A 848 THR LEU VAL LYS ASP ARG ASN VAL ALA THR THR ARG ASP
SEQRES 5 A 848 TYR TYR PHE ALA LEU ALA HIS THR VAL ARG ASP HIS LEU
SEQRES 6 A 848 VAL GLY ARG TRP ILE ARG THR GLN GLN HIS TYR TYR ASP
SEQRES 7 A 848 LYS CYS PRO LYS ARG VAL TYR TYR LEU SER LEU GLU PHE
SEQRES 8 A 848 TYR MET GLY ARG THR LEU GLN ASN THR MET ILE ASN LEU
SEQRES 9 A 848 GLY LEU GLN ASN ALA CYS ASP GLU ALA ILE TYR GLN LEU
SEQRES 10 A 848 GLY LEU ASP ILE GLU GLU LEU GLU GLU ILE GLU GLU ASP
SEQRES 11 A 848 ALA GLY LEU GLY ASN GLY GLY LEU GLY ARG LEU ALA ALA
SEQRES 12 A 848 CYS PHE LEU ASP SER MET ALA THR LEU GLY LEU ALA ALA
SEQRES 13 A 848 TYR GLY TYR GLY ILE ARG TYR GLU TYR GLY ILE PHE ASN
SEQRES 14 A 848 GLN LYS ILE ARG ASP GLY TRP GLN VAL GLU GLU ALA ASP
SEQRES 15 A 848 ASP TRP LEU ARG TYR GLY ASN PRO TRP GLU LYS SER ARG
SEQRES 16 A 848 PRO GLU PHE MET LEU PRO VAL HIS PHE TYR GLY LYS VAL
SEQRES 17 A 848 GLU HIS THR ASN THR GLY THR LYS TRP ILE ASP THR GLN
SEQRES 18 A 848 VAL VAL LEU ALA LEU PRO TYR ASP THR PRO VAL PRO GLY
SEQRES 19 A 848 TYR MET ASN ASN THR VAL ASN THR MET ARG LEU TRP SER
SEQRES 20 A 848 ALA ARG ALA PRO ASN ASP PHE ASN LEU ARG ASP PHE ASN
SEQRES 21 A 848 VAL GLY ASP TYR ILE GLN ALA VAL LEU ASP ARG ASN LEU
SEQRES 22 A 848 ALA GLU ASN ILE SER ARG VAL LEU TYR PRO ASN ASP ASN
SEQRES 23 A 848 PHE PHE GLU GLY LYS GLU LEU ARG LEU LYS GLN GLU TYR
SEQRES 24 A 848 PHE VAL VAL ALA ALA THR LEU GLN ASP ILE ILE ARG ARG
SEQRES 25 A 848 PHE LYS ALA SER LYS PHE GLY SER THR ARG GLY ALA GLY
SEQRES 26 A 848 THR VAL PHE ASP ALA PHE PRO ASP GLN VAL ALA ILE GLN
SEQRES 27 A 848 LEU ASN ASP THR HIS PRO ALA LEU ALA ILE PRO GLU LEU
SEQRES 28 A 848 MET ARG ILE PHE VAL ASP ILE GLU LYS LEU PRO TRP SER
SEQRES 29 A 848 LYS ALA TRP GLU LEU THR GLN LYS THR PHE ALA TYR THR
SEQRES 30 A 848 ASN HIS THR VAL LEU PRO GLU ALA LEU GLU ARG TRP PRO
SEQRES 31 A 848 VAL ASP LEU VAL GLU LYS LEU LEU PRO ARG HIS LEU GLU
SEQRES 32 A 848 ILE ILE TYR GLU ILE ASN GLN LYS HIS LEU ASP ARG ILE
SEQRES 33 A 848 VAL ALA LEU PHE PRO LYS ASP VAL ASP ARG LEU ARG ARG
SEQRES 34 A 848 MET SER LEU ILE GLU GLU GLU GLY SER LYS ARG ILE ASN
SEQRES 35 A 848 MET ALA HIS LEU CYS ILE VAL GLY SER HIS ALA VAL ASN
SEQRES 36 A 848 GLY VAL ALA LYS ILE HIS SER ASP ILE VAL LYS THR LYS
SEQRES 37 A 848 VAL PHE LYS ASP PHE SER GLU LEU GLU PRO ASP LYS PHE
SEQRES 38 A 848 GLN ASN LYS THR ASN GLY ILE THR PRO ARG ARG TRP LEU
SEQRES 39 A 848 LEU LEU CYS ASN PRO GLY LEU ALA GLU LEU ILE ALA GLU
SEQRES 40 A 848 LYS ILE GLY GLU ASP TYR VAL LYS ASP LEU SER GLN LEU
SEQRES 41 A 848 THR LYS LEU HIS SER PHE LEU GLY ASP ASP VAL PHE LEU
SEQRES 42 A 848 ARG GLU LEU ALA LYS VAL LYS GLN GLU ASN LYS LEU LYS
SEQRES 43 A 848 PHE SER GLN PHE LEU GLU THR GLU TYR LYS VAL LYS ILE
SEQRES 44 A 848 ASN PRO SER SER MET PHE ASP VAL GLN VAL LYS ARG ILE
SEQRES 45 A 848 HIS GLU TYR LYS ARG GLN LEU LEU ASN CYS LEU HIS VAL
SEQRES 46 A 848 ILE THR MET TYR ASN ARG ILE LYS LYS ASP PRO LYS LYS
SEQRES 47 A 848 LEU PHE VAL PRO ARG THR VAL ILE ILE GLY GLY LYS ALA
SEQRES 48 A 848 ALA PRO GLY TYR HIS MET ALA LYS MET ILE ILE LYS LEU
SEQRES 49 A 848 ILE THR SER VAL ALA ASP VAL VAL ASN ASN ASP PRO MET
SEQRES 50 A 848 VAL GLY SER LYS LEU LYS VAL ILE PHE LEU GLU ASN TYR
SEQRES 51 A 848 ARG VAL SER LEU ALA GLU LYS VAL ILE PRO ALA THR ASP
SEQRES 52 A 848 LEU SER GLU GLN ILE SER THR ALA GLY THR GLU ALA SER
SEQRES 53 A 848 GLY THR GLY ASN MET LYS PHE MET LEU ASN GLY ALA LEU
SEQRES 54 A 848 THR ILE GLY THR MET ASP GLY ALA ASN VAL GLU MET ALA
SEQRES 55 A 848 GLU GLU ALA GLY GLU GLU ASN LEU PHE ILE PHE GLY MET
SEQRES 56 A 848 ARG ILE ASP ASP VAL ALA ALA LEU ASP LYS LYS GLY TYR
SEQRES 57 A 848 GLU ALA LYS GLU TYR TYR GLU ALA LEU PRO GLU LEU LYS
SEQRES 58 A 848 LEU VAL ILE ASP GLN ILE ASP ASN GLY PHE PHE SER PRO
SEQRES 59 A 848 LYS GLN PRO ASP LEU PHE LYS ASP ILE ILE ASN MET LEU
SEQRES 60 A 848 PHE TYR HIS ASP ARG PHE LYS VAL PHE ALA ASP TYR GLU
SEQRES 61 A 848 ALA TYR VAL LYS CYS GLN ASP LYS VAL SER GLN LEU TYR
SEQRES 62 A 848 MET ASN PRO LYS ALA TRP ASN THR MET VAL LEU LYS ASN
SEQRES 63 A 848 ILE ALA ALA SER GLY LYS PHE SER SER ASP ARG THR ILE
SEQRES 64 A 848 LYS GLU TYR ALA GLN ASN ILE TRP ASN VAL GLU PRO SER
SEQRES 65 A 848 ASP LEU LYS ILE SER LEU SER ASN GLU SER ASN LYS VAL
SEQRES 66 A 848 ASN GLY ASN
SEQRES 1 B 848 GLY GLY ALA LYS PRO LEU THR ASP GLN GLU LYS ARG ARG
SEQRES 2 B 848 GLN ILE SEP ILE ARG GLY ILE VAL GLY VAL GLU ASN VAL
SEQRES 3 B 848 ALA GLU LEU LYS LYS SER PHE ASN ARG HIS LEU HIS PHE
SEQRES 4 B 848 THR LEU VAL LYS ASP ARG ASN VAL ALA THR THR ARG ASP
SEQRES 5 B 848 TYR TYR PHE ALA LEU ALA HIS THR VAL ARG ASP HIS LEU
SEQRES 6 B 848 VAL GLY ARG TRP ILE ARG THR GLN GLN HIS TYR TYR ASP
SEQRES 7 B 848 LYS CYS PRO LYS ARG VAL TYR TYR LEU SER LEU GLU PHE
SEQRES 8 B 848 TYR MET GLY ARG THR LEU GLN ASN THR MET ILE ASN LEU
SEQRES 9 B 848 GLY LEU GLN ASN ALA CYS ASP GLU ALA ILE TYR GLN LEU
SEQRES 10 B 848 GLY LEU ASP ILE GLU GLU LEU GLU GLU ILE GLU GLU ASP
SEQRES 11 B 848 ALA GLY LEU GLY ASN GLY GLY LEU GLY ARG LEU ALA ALA
SEQRES 12 B 848 CYS PHE LEU ASP SER MET ALA THR LEU GLY LEU ALA ALA
SEQRES 13 B 848 TYR GLY TYR GLY ILE ARG TYR GLU TYR GLY ILE PHE ASN
SEQRES 14 B 848 GLN LYS ILE ARG ASP GLY TRP GLN VAL GLU GLU ALA ASP
SEQRES 15 B 848 ASP TRP LEU ARG TYR GLY ASN PRO TRP GLU LYS SER ARG
SEQRES 16 B 848 PRO GLU PHE MET LEU PRO VAL HIS PHE TYR GLY LYS VAL
SEQRES 17 B 848 GLU HIS THR ASN THR GLY THR LYS TRP ILE ASP THR GLN
SEQRES 18 B 848 VAL VAL LEU ALA LEU PRO TYR ASP THR PRO VAL PRO GLY
SEQRES 19 B 848 TYR MET ASN ASN THR VAL ASN THR MET ARG LEU TRP SER
SEQRES 20 B 848 ALA ARG ALA PRO ASN ASP PHE ASN LEU ARG ASP PHE ASN
SEQRES 21 B 848 VAL GLY ASP TYR ILE GLN ALA VAL LEU ASP ARG ASN LEU
SEQRES 22 B 848 ALA GLU ASN ILE SER ARG VAL LEU TYR PRO ASN ASP ASN
SEQRES 23 B 848 PHE PHE GLU GLY LYS GLU LEU ARG LEU LYS GLN GLU TYR
SEQRES 24 B 848 PHE VAL VAL ALA ALA THR LEU GLN ASP ILE ILE ARG ARG
SEQRES 25 B 848 PHE LYS ALA SER LYS PHE GLY SER THR ARG GLY ALA GLY
SEQRES 26 B 848 THR VAL PHE ASP ALA PHE PRO ASP GLN VAL ALA ILE GLN
SEQRES 27 B 848 LEU ASN ASP THR HIS PRO ALA LEU ALA ILE PRO GLU LEU
SEQRES 28 B 848 MET ARG ILE PHE VAL ASP ILE GLU LYS LEU PRO TRP SER
SEQRES 29 B 848 LYS ALA TRP GLU LEU THR GLN LYS THR PHE ALA TYR THR
SEQRES 30 B 848 ASN HIS THR VAL LEU PRO GLU ALA LEU GLU ARG TRP PRO
SEQRES 31 B 848 VAL ASP LEU VAL GLU LYS LEU LEU PRO ARG HIS LEU GLU
SEQRES 32 B 848 ILE ILE TYR GLU ILE ASN GLN LYS HIS LEU ASP ARG ILE
SEQRES 33 B 848 VAL ALA LEU PHE PRO LYS ASP VAL ASP ARG LEU ARG ARG
SEQRES 34 B 848 MET SER LEU ILE GLU GLU GLU GLY SER LYS ARG ILE ASN
SEQRES 35 B 848 MET ALA HIS LEU CYS ILE VAL GLY SER HIS ALA VAL ASN
SEQRES 36 B 848 GLY VAL ALA LYS ILE HIS SER ASP ILE VAL LYS THR LYS
SEQRES 37 B 848 VAL PHE LYS ASP PHE SER GLU LEU GLU PRO ASP LYS PHE
SEQRES 38 B 848 GLN ASN LYS THR ASN GLY ILE THR PRO ARG ARG TRP LEU
SEQRES 39 B 848 LEU LEU CYS ASN PRO GLY LEU ALA GLU LEU ILE ALA GLU
SEQRES 40 B 848 LYS ILE GLY GLU ASP TYR VAL LYS ASP LEU SER GLN LEU
SEQRES 41 B 848 THR LYS LEU HIS SER PHE LEU GLY ASP ASP VAL PHE LEU
SEQRES 42 B 848 ARG GLU LEU ALA LYS VAL LYS GLN GLU ASN LYS LEU LYS
SEQRES 43 B 848 PHE SER GLN PHE LEU GLU THR GLU TYR LYS VAL LYS ILE
SEQRES 44 B 848 ASN PRO SER SER MET PHE ASP VAL GLN VAL LYS ARG ILE
SEQRES 45 B 848 HIS GLU TYR LYS ARG GLN LEU LEU ASN CYS LEU HIS VAL
SEQRES 46 B 848 ILE THR MET TYR ASN ARG ILE LYS LYS ASP PRO LYS LYS
SEQRES 47 B 848 LEU PHE VAL PRO ARG THR VAL ILE ILE GLY GLY LYS ALA
SEQRES 48 B 848 ALA PRO GLY TYR HIS MET ALA LYS MET ILE ILE LYS LEU
SEQRES 49 B 848 ILE THR SER VAL ALA ASP VAL VAL ASN ASN ASP PRO MET
SEQRES 50 B 848 VAL GLY SER LYS LEU LYS VAL ILE PHE LEU GLU ASN TYR
SEQRES 51 B 848 ARG VAL SER LEU ALA GLU LYS VAL ILE PRO ALA THR ASP
SEQRES 52 B 848 LEU SER GLU GLN ILE SER THR ALA GLY THR GLU ALA SER
SEQRES 53 B 848 GLY THR GLY ASN MET LYS PHE MET LEU ASN GLY ALA LEU
SEQRES 54 B 848 THR ILE GLY THR MET ASP GLY ALA ASN VAL GLU MET ALA
SEQRES 55 B 848 GLU GLU ALA GLY GLU GLU ASN LEU PHE ILE PHE GLY MET
SEQRES 56 B 848 ARG ILE ASP ASP VAL ALA ALA LEU ASP LYS LYS GLY TYR
SEQRES 57 B 848 GLU ALA LYS GLU TYR TYR GLU ALA LEU PRO GLU LEU LYS
SEQRES 58 B 848 LEU VAL ILE ASP GLN ILE ASP ASN GLY PHE PHE SER PRO
SEQRES 59 B 848 LYS GLN PRO ASP LEU PHE LYS ASP ILE ILE ASN MET LEU
SEQRES 60 B 848 PHE TYR HIS ASP ARG PHE LYS VAL PHE ALA ASP TYR GLU
SEQRES 61 B 848 ALA TYR VAL LYS CYS GLN ASP LYS VAL SER GLN LEU TYR
SEQRES 62 B 848 MET ASN PRO LYS ALA TRP ASN THR MET VAL LEU LYS ASN
SEQRES 63 B 848 ILE ALA ALA SER GLY LYS PHE SER SER ASP ARG THR ILE
SEQRES 64 B 848 LYS GLU TYR ALA GLN ASN ILE TRP ASN VAL GLU PRO SER
SEQRES 65 B 848 ASP LEU LYS ILE SER LEU SER ASN GLU SER ASN LYS VAL
SEQRES 66 B 848 ASN GLY ASN
MODRES 3DDW SEP A 14 SER PHOSPHOSERINE
HET SEP A 14 20
HET NBG A 901 15
HET NBG B 901 15
HET PLP A 902 15
HET CFF A 903 14
HET 055 A 905 35
HET PLP B 902 15
HET CFF B 903 14
HET MES B 904 12
HET MES B 905 12
HET 055 B 906 35
HET MPD A 906 8
HET MPD B 907 8
HETNAM SEP PHOSPHOSERINE
HETNAM NBG 1-N-ACETYL-BETA-D-GLUCOSAMINE
HETNAM PLP PYRIDOXAL-5'-PHOSPHATE
HETNAM CFF CAFFEINE
HETNAM 055 (2S)-{[(3-{[(2-CHLORO-6-METHYLPHENYL)
HETNAM 2 055 CARBAMOYL]AMINO}NAPHTHALEN-2-YL)
HETNAM 3 055 CARBONYL]AMINO}(PHENYL)ETHANOIC ACID
HETNAM MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID
HETNAM MPD (4S)-2-METHYL-2,4-PENTANEDIOL
HETSYN SEP PHOSPHONOSERINE
HETSYN PLP VITAMIN B6 PHOSPHATE
HETSYN CFF 3,7-DIHYDRO-1,3,7-TRIMETHYL-1H-PURINE-2,6-DIONE
FORMUL 1 SEP C3 H8 N O6 P
FORMUL 3 NBG 2(C8 H15 N O6)
FORMUL 5 PLP 2(C8 H10 N O6 P)
FORMUL 6 CFF 2(C8 H10 N4 O2)
FORMUL 7 055 2(C27 H22 CL N3 O4)
FORMUL 10 MES 2(C6 H13 N O4 S)
FORMUL 13 MPD 2(C6 H14 O2)
FORMUL 15 HOH *1612(H2 O)
HELIX 1 1 ARG A 16 THR A 38 1 23
HELIX 2 2 ASP A 42 ALA A 46 5 5
HELIX 3 3 THR A 47 HIS A 62 1 16
HELIX 4 4 LEU A 63 CYS A 78 1 16
HELIX 5 5 THR A 94 LEU A 102 1 9
HELIX 6 6 LEU A 104 LEU A 115 1 12
HELIX 7 7 ASP A 118 GLU A 126 1 9
HELIX 8 8 GLY A 134 LEU A 150 1 17
HELIX 9 9 PRO A 194 MET A 197 5 4
HELIX 10 10 ASP A 261 ASP A 268 1 8
HELIX 11 11 ARG A 269 ASN A 274 1 6
HELIX 12 12 ILE A 275 ARG A 277 5 3
HELIX 13 13 LYS A 289 SER A 314 1 26
HELIX 14 14 ALA A 328 GLN A 332 1 5
HELIX 15 15 LEU A 344 ILE A 356 1 13
HELIX 16 16 PRO A 360 THR A 371 1 12
HELIX 17 17 LEU A 380 LEU A 384 5 5
HELIX 18 18 VAL A 389 LEU A 396 1 8
HELIX 19 19 LEU A 396 PHE A 418 1 23
HELIX 20 20 ASP A 421 SER A 429 1 9
HELIX 21 21 MET A 441 GLY A 448 1 8
HELIX 22 22 ALA A 456 LYS A 466 1 11
HELIX 23 23 PHE A 468 GLU A 475 1 8
HELIX 24 24 ASN A 496 GLY A 508 1 13
HELIX 25 25 ASP A 514 LEU A 525 5 12
HELIX 26 26 ASP A 527 TYR A 553 1 27
HELIX 27 27 ARG A 575 ASP A 593 1 19
HELIX 28 28 TYR A 613 ASP A 633 1 21
HELIX 29 29 VAL A 636 SER A 638 5 3
HELIX 30 30 ARG A 649 ILE A 657 1 9
HELIX 31 31 PRO A 658 THR A 660 5 3
HELIX 32 32 THR A 676 ASN A 684 1 9
HELIX 33 33 ALA A 695 GLY A 704 1 10
HELIX 34 34 GLU A 705 LEU A 708 5 4
HELIX 35 35 ARG A 714 GLY A 725 1 12
HELIX 36 36 GLU A 727 LEU A 735 1 9
HELIX 37 37 LEU A 735 GLY A 748 1 14
HELIX 38 38 PHE A 758 HIS A 768 1 11
HELIX 39 39 LYS A 772 MET A 792 1 21
HELIX 40 40 ASN A 793 ALA A 807 1 15
HELIX 41 41 SER A 808 PHE A 811 5 4
HELIX 42 42 SER A 812 ILE A 824 1 13
HELIX 43 43 ASN B 23 THR B 38 1 16
HELIX 44 44 ASP B 42 ALA B 46 5 5
HELIX 45 45 THR B 47 HIS B 62 1 16
HELIX 46 46 LEU B 63 CYS B 78 1 16
HELIX 47 47 THR B 94 LEU B 102 1 9
HELIX 48 48 LEU B 104 LEU B 115 1 12
HELIX 49 49 ASP B 118 GLU B 124 1 7
HELIX 50 50 GLY B 134 LEU B 150 1 17
HELIX 51 51 PRO B 194 MET B 197 5 4
HELIX 52 52 ASP B 261 ASP B 268 1 8
HELIX 53 53 ASP B 268 ASN B 274 1 7
HELIX 54 54 ILE B 275 ARG B 277 5 3
HELIX 55 55 LYS B 289 SER B 314 1 26
HELIX 56 56 ALA B 328 GLN B 332 1 5
HELIX 57 57 LEU B 344 ILE B 356 1 13
HELIX 58 58 PRO B 360 THR B 371 1 12
HELIX 59 59 LEU B 380 LEU B 384 5 5
HELIX 60 60 VAL B 389 LEU B 396 1 8
HELIX 61 61 LEU B 396 PHE B 418 1 23
HELIX 62 62 ASP B 421 SER B 429 1 9
HELIX 63 63 MET B 441 SER B 449 1 9
HELIX 64 64 ALA B 456 LYS B 466 1 11
HELIX 65 65 PHE B 468 GLU B 475 1 8
HELIX 66 66 ASN B 496 GLY B 508 1 13
HELIX 67 67 GLU B 509 LYS B 513 5 5
HELIX 68 68 ASP B 514 LEU B 525 5 12
HELIX 69 69 ASP B 527 LYS B 554 1 28
HELIX 70 70 ARG B 575 ASP B 593 1 19
HELIX 71 71 TYR B 613 ASP B 633 1 21
HELIX 72 72 VAL B 636 SER B 638 5 3
HELIX 73 73 ARG B 649 ILE B 657 1 9
HELIX 74 74 PRO B 658 THR B 660 5 3
HELIX 75 75 THR B 676 ASN B 684 1 9
HELIX 76 76 ALA B 695 GLY B 704 1 10
HELIX 77 77 GLU B 705 LEU B 708 5 4
HELIX 78 78 ARG B 714 GLY B 725 1 12
HELIX 79 79 GLU B 727 LEU B 735 1 9
HELIX 80 80 LEU B 735 ASN B 747 1 13
HELIX 81 81 PHE B 758 HIS B 768 1 11
HELIX 82 82 LYS B 772 MET B 792 1 21
HELIX 83 83 ASN B 793 ALA B 807 1 15
HELIX 84 84 SER B 808 PHE B 811 5 4
HELIX 85 85 SER B 812 ILE B 824 1 13
SHEET 1 A 3 LYS A 191 SER A 192 0
SHEET 2 A 3 GLN A 219 PRO A 231 -1 O ASP A 227 N LYS A 191
SHEET 3 A 3 LEU A 198 PHE A 202 -1 N PHE A 202 O GLN A 219
SHEET 1 B 9 LYS A 191 SER A 192 0
SHEET 2 B 9 GLN A 219 PRO A 231 -1 O ASP A 227 N LYS A 191
SHEET 3 B 9 VAL A 238 ARG A 247 -1 O ARG A 247 N LEU A 222
SHEET 4 B 9 ALA A 154 ILE A 159 1 N GLY A 156 O ARG A 242
SHEET 5 B 9 ARG A 81 LEU A 85 1 N TYR A 84 O TYR A 155
SHEET 6 B 9 VAL A 333 ASN A 338 1 O ALA A 334 N TYR A 83
SHEET 7 B 9 PHE A 372 THR A 375 1 O ALA A 373 N LEU A 337
SHEET 8 B 9 ALA A 451 GLY A 454 1 O ALA A 451 N TYR A 374
SHEET 9 B 9 PHE A 479 ASN A 481 1 O GLN A 480 N VAL A 452
SHEET 1 C 2 PHE A 89 GLY A 92 0
SHEET 2 C 2 ALA A 129 LEU A 131 -1 O ALA A 129 N GLY A 92
SHEET 1 D 2 ASN A 167 ARG A 171 0
SHEET 2 D 2 TRP A 174 GLU A 178 -1 O TRP A 174 N ARG A 171
SHEET 1 E 2 LYS A 205 THR A 209 0
SHEET 2 E 2 GLY A 212 ILE A 216 -1 O ILE A 216 N LYS A 205
SHEET 1 F 3 ARG A 386 PRO A 388 0
SHEET 2 F 3 ARG A 438 ASN A 440 -1 O ILE A 439 N TRP A 387
SHEET 3 F 3 ILE A 431 GLU A 432 -1 N GLU A 432 O ARG A 438
SHEET 1 G 6 LEU A 640 LEU A 645 0
SHEET 2 G 6 ARG A 601 GLY A 606 1 N VAL A 603 O LYS A 641
SHEET 3 G 6 MET A 562 VAL A 567 1 N ASP A 564 O ILE A 604
SHEET 4 G 6 LEU A 662 GLN A 665 1 O LEU A 662 N VAL A 565
SHEET 5 G 6 LEU A 687 GLY A 690 1 O LEU A 687 N SER A 663
SHEET 6 G 6 PHE A 709 ILE A 710 1 O PHE A 709 N THR A 688
SHEET 1 H 3 LYS B 191 SER B 192 0
SHEET 2 H 3 GLN B 219 PRO B 231 -1 O ASP B 227 N LYS B 191
SHEET 3 H 3 LEU B 198 PHE B 202 -1 N LEU B 198 O ALA B 223
SHEET 1 I 9 LYS B 191 SER B 192 0
SHEET 2 I 9 GLN B 219 PRO B 231 -1 O ASP B 227 N LYS B 191
SHEET 3 I 9 VAL B 238 ARG B 247 -1 O SER B 245 N LEU B 224
SHEET 4 I 9 ALA B 154 ILE B 159 1 N GLY B 156 O ARG B 242
SHEET 5 I 9 ARG B 81 LEU B 85 1 N TYR B 84 O TYR B 157
SHEET 6 I 9 VAL B 333 ASN B 338 1 O ALA B 334 N TYR B 83
SHEET 7 I 9 PHE B 372 THR B 375 1 O ALA B 373 N LEU B 337
SHEET 8 I 9 ALA B 451 GLY B 454 1 O ALA B 451 N TYR B 374
SHEET 9 I 9 PHE B 479 ASN B 481 1 O GLN B 480 N VAL B 452
SHEET 1 J 2 PHE B 89 GLY B 92 0
SHEET 2 J 2 ALA B 129 LEU B 131 -1 O ALA B 129 N GLY B 92
SHEET 1 K 2 ASN B 167 ARG B 171 0
SHEET 2 K 2 TRP B 174 GLU B 178 -1 O TRP B 174 N ARG B 171
SHEET 1 L 2 LYS B 205 THR B 209 0
SHEET 2 L 2 GLY B 212 ILE B 216 -1 O LYS B 214 N GLU B 207
SHEET 1 M 3 ARG B 386 PRO B 388 0
SHEET 2 M 3 ARG B 438 ASN B 440 -1 O ILE B 439 N TRP B 387
SHEET 3 M 3 ILE B 431 GLU B 432 -1 N GLU B 432 O ARG B 438
SHEET 1 N 6 LEU B 640 LEU B 645 0
SHEET 2 N 6 ARG B 601 GLY B 606 1 N VAL B 603 O LYS B 641
SHEET 3 N 6 MET B 562 VAL B 567 1 N ASP B 564 O ILE B 604
SHEET 4 N 6 LEU B 662 GLN B 665 1 O LEU B 662 N VAL B 565
SHEET 5 N 6 LEU B 687 GLY B 690 1 O LEU B 687 N SER B 663
SHEET 6 N 6 PHE B 709 ILE B 710 1 O PHE B 709 N THR B 688
LINK C ILE A 13 N ASEP A 14 1555 1555 1.33
LINK C ILE A 13 N BSEP A 14 1555 1555 1.33
LINK C ASEP A 14 N ILE A 15 1555 1555 1.33
LINK C BSEP A 14 N ILE A 15 1555 1555 1.33
LINK C4A PLP A 902 NZ LYS A 680 1555 1555 1.27
LINK C4A PLP B 902 NZ LYS B 680 1555 1555 1.27
SITE 1 AC1 11 LEU A 136 ASN A 284 ASP A 339 HIS A 377
SITE 2 AC1 11 THR A 378 ASN A 484 TYR A 573 GLU A 672
SITE 3 AC1 11 ALA A 673 SER A 674 GLY A 675
SITE 1 AC2 10 LEU B 136 ASN B 284 ASP B 339 HIS B 377
SITE 2 AC2 10 ASN B 484 TYR B 573 GLU B 672 ALA B 673
SITE 3 AC2 10 SER B 674 GLY B 675
SITE 1 AC3 11 TYR A 90 GLY A 134 TRP A 491 LYS A 568
SITE 2 AC3 11 LYS A 574 TYR A 648 ARG A 649 GLY A 675
SITE 3 AC3 11 THR A 676 GLY A 677 LYS A 680
SITE 1 AC4 11 TYR B 90 GLY B 134 TRP B 491 LYS B 568
SITE 2 AC4 11 LYS B 574 TYR B 648 ARG B 649 GLY B 675
SITE 3 AC4 11 THR B 676 GLY B 677 LYS B 680
SITE 1 AC5 6 ASN A 282 PHE A 285 HIS A 571 ALA A 610
SITE 2 AC5 6 GLY A 612 TYR A 613
SITE 1 AC6 6 ASN B 282 PHE B 285 HIS B 571 ALA B 610
SITE 2 AC6 6 GLY B 612 TYR B 613
SITE 1 AC7 6 ILE B 170 ASP B 172 GLY B 173 GLU B 552
SITE 2 AC7 6 TYR B 553 GLU B 646
SITE 1 AC8 7 ARG B 49 ARG B 93 GLU B 124 ILE B 125
SITE 2 AC8 7 GLU B 126 GLU B 127 GLY B 526
SITE 1 AC9 8 TRP A 67 GLN A 71 GLN A 72 ARG A 193
SITE 2 AC9 8 ASP A 227 ARG A 310 LYS B 41 ASP B 42
SITE 1 BC1 7 LYS A 41 ASP A 42 GLN B 71 GLN B 72
SITE 2 BC1 7 ARG B 193 ASP B 227 ARG B 310
SITE 1 BC2 5 ALA A 322 ILE A 356 LYS A 358 GLU B 509
SITE 2 BC2 5 TYR B 511
SITE 1 BC3 2 HIS A 201 ARG A 351
CRYST1 124.368 124.368 123.621 90.00 90.00 120.00 P 31 6
ORIGX1 1.000000 0.000000 0.000000 0.00000
ORIGX2 0.000000 1.000000 0.000000 0.00000
ORIGX3 0.000000 0.000000 1.000000 0.00000
SCALE1 0.008041 0.004642 0.000000 0.00000
SCALE2 0.000000 0.009285 0.000000 0.00000
SCALE3 0.000000 0.000000 0.008089 0.00000
(ATOM LINES ARE NOT SHOWN.)
END
