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Database: PDB
Entry: 3NH2
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Original site: 3NH2 
HEADER    HYDROLASE/DNA                           14-JUN-10   3NH2              
TITLE     CRYSTAL STRUCTURE OF RNASE T IN COMPLEX WITH A STEM DNA WITH A 3'     
TITLE    2 OVERHANG                                                             
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: RIBONUCLEASE T;                                            
COMPND   3 CHAIN: A, B, E, F;                                                   
COMPND   4 SYNONYM: RNASE T, EXORIBONUCLEASE T;                                 
COMPND   5 EC: 3.1.13.-;                                                        
COMPND   6 ENGINEERED: YES;                                                     
COMPND   7 MOL_ID: 2;                                                           
COMPND   8 MOLECULE: 5'-D(P*TP*TP*AP*CP*AP*AP*C)-3';                            
COMPND   9 CHAIN: C, D, G, H;                                                   
COMPND  10 ENGINEERED: YES;                                                     
COMPND  11 OTHER_DETAILS: SSDNA                                                 
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: ESCHERICHIA COLI;                               
SOURCE   3 ORGANISM_TAXID: 562;                                                 
SOURCE   4 STRAIN: JM109 / ATCC 53323;                                          
SOURCE   5 GENE: RNT;                                                           
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE   8 EXPRESSION_SYSTEM_STRAIN: RIPL;                                      
SOURCE   9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  10 EXPRESSION_SYSTEM_PLASMID: PET28A;                                   
SOURCE  11 MOL_ID: 2;                                                           
SOURCE  12 SYNTHETIC: YES;                                                      
SOURCE  13 ORGANISM_SCIENTIFIC: SYNTHETIC CONSTRUCT;                            
SOURCE  14 ORGANISM_TAXID: 32630                                                
KEYWDS    EXORIBONUCLEASE, RNA PROCESSING, RNA MATURATION, PROTEIN-DNA          
KEYWDS   2 INTERACTIONS, PROTEIN-DNA COMPLEX, EXO-NUCLEASE, HYDROLASE-DNA       
KEYWDS   3 COMPLEX                                                              
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    Y.-Y.HSIAO,H.S.YUAN                                                   
REVDAT   5   08-NOV-23 3NH2    1       REMARK                                   
REVDAT   4   31-MAY-23 3NH2    1       SOURCE REMARK SEQADV                     
REVDAT   3   21-DEC-11 3NH2    1       JRNL   VERSN                             
REVDAT   2   23-FEB-11 3NH2    1       JRNL                                     
REVDAT   1   16-FEB-11 3NH2    0                                                
JRNL        AUTH   Y.-Y.HSIAO,C.-C.YANG,C.L.LIN,J.L.J.LIN,Y.DUH,H.S.YUAN        
JRNL        TITL   STRUCTURAL BASIS FOR RNA TRIMMING BY RNASE T IN STABLE RNA   
JRNL        TITL 2 3'-END MATURATION                                            
JRNL        REF    NAT.CHEM.BIOL.                V.   7   236 2011              
JRNL        REFN                   ISSN 1552-4450                               
JRNL        PMID   21317904                                                     
JRNL        DOI    10.1038/NCHEMBIO.524                                         
REMARK   2                                                                      
REMARK   2 RESOLUTION.    2.30 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX (PHENIX.REFINE: 1.6_289)                      
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : ML                                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.30                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 26.76                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.970                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 96.3                           
REMARK   3   NUMBER OF REFLECTIONS             : 35456                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.190                           
REMARK   3   R VALUE            (WORKING SET) : 0.186                           
REMARK   3   FREE R VALUE                     : 0.241                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 7.600                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 2693                            
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 26.7623 -  6.1142    0.95     1677   149  0.1963 0.2285        
REMARK   3     2  6.1142 -  4.8625    0.94     1674   136  0.1745 0.1832        
REMARK   3     3  4.8625 -  4.2506    0.96     1732   154  0.1455 0.1918        
REMARK   3     4  4.2506 -  3.8632    0.96     1710   147  0.1662 0.1939        
REMARK   3     5  3.8632 -  3.5870    0.95     1723   133  0.1554 0.2112        
REMARK   3     6  3.5870 -  3.3760    0.97     1714   147  0.1656 0.2052        
REMARK   3     7  3.3760 -  3.2072    0.97     1750   147  0.1714 0.2269        
REMARK   3     8  3.2072 -  3.0678    0.97     1735   138  0.1801 0.2378        
REMARK   3     9  3.0678 -  2.9498    0.96     1716   149  0.1858 0.2462        
REMARK   3    10  2.9498 -  2.8482    0.97     1731   135  0.1922 0.2762        
REMARK   3    11  2.8482 -  2.7592    0.97     1755   142  0.2050 0.2988        
REMARK   3    12  2.7592 -  2.6804    0.97     1751   143  0.2039 0.2880        
REMARK   3    13  2.6804 -  2.6099    0.97     1704   118  0.2020 0.2648        
REMARK   3    14  2.6099 -  2.5463    0.97     1761   154  0.2053 0.2744        
REMARK   3    15  2.5463 -  2.4884    0.96     1718   137  0.2128 0.3434        
REMARK   3    16  2.4884 -  2.4355    0.96     1759   139  0.2025 0.2851        
REMARK   3    17  2.4355 -  2.3868    0.97     1723   140  0.2083 0.2640        
REMARK   3    18  2.3868 -  2.3418    0.97     1733   146  0.2059 0.3308        
REMARK   3    19  2.3418 -  2.3000    0.95     1697   139  0.1983 0.2658        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL                       
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : 0.40                                          
REMARK   3   B_SOL              : 40.97                                         
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.340            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 26.720           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 26.23                          
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : 1.15700                                              
REMARK   3    B22 (A**2) : -1.08760                                             
REMARK   3    B33 (A**2) : -0.06940                                             
REMARK   3    B12 (A**2) : -0.55410                                             
REMARK   3    B13 (A**2) : -0.58550                                             
REMARK   3    B23 (A**2) : 2.47370                                              
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.003           7023                                  
REMARK   3   ANGLE     :  0.736           9618                                  
REMARK   3   CHIRALITY :  0.050           1064                                  
REMARK   3   PLANARITY :  0.002           1176                                  
REMARK   3   DIHEDRAL  : 17.435           2514                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : NULL                                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 3NH2 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 21-JUN-10.                  
REMARK 100 THE DEPOSITION ID IS D_1000059819.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 14-NOV-09                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 5.5                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : NSRRC                              
REMARK 200  BEAMLINE                       : BL13B1                             
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.999                              
REMARK 200  MONOCHROMATOR                  : SAGITALLY FOCUSED SI(111)          
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : ADSC QUANTUM 315                   
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : HKL-2000                           
REMARK 200  DATA SCALING SOFTWARE          : HKL-2000                           
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 35579                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.300                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 30.000                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : 0.000                              
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 96.5                               
REMARK 200  DATA REDUNDANCY                : 2.600                              
REMARK 200  R MERGE                    (I) : NULL                               
REMARK 200  R SYM                      (I) : 0.10000                            
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 9.7000                             
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.30                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.38                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 96.5                               
REMARK 200  DATA REDUNDANCY IN SHELL       : 2.50                               
REMARK 200  R MERGE FOR SHELL          (I) : NULL                               
REMARK 200  R SYM FOR SHELL            (I) : 0.41000                            
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 2.400                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: AMORE                                                 
REMARK 200 STARTING MODEL: PDB ENTRY 3NGY                                       
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 36.09                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 1.92                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.1% W/V N-OCTYL-BETA-D-GLUCOSIDE,       
REMARK 280  0.1M SODIUM CITRATE TRIBASIC DIHYDRATE, PH 5.5, 22% PEG 4000,       
REMARK 280  VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K                     
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 1                              
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1, 2, 3                                                 
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TETRAMERIC                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, C, D                            
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 2                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: OCTAMERIC                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, C, D, E, F, G, H                
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 3                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TETRAMERIC                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: E, F, G, H                            
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     MET A   -19                                                      
REMARK 465     GLY A   -18                                                      
REMARK 465     SER A   -17                                                      
REMARK 465     SER A   -16                                                      
REMARK 465     HIS A   -15                                                      
REMARK 465     HIS A   -14                                                      
REMARK 465     HIS A   -13                                                      
REMARK 465     HIS A   -12                                                      
REMARK 465     HIS A   -11                                                      
REMARK 465     HIS A   -10                                                      
REMARK 465     SER A    -9                                                      
REMARK 465     SER A    -8                                                      
REMARK 465     GLY A    -7                                                      
REMARK 465     LEU A    -6                                                      
REMARK 465     VAL A    -5                                                      
REMARK 465     PRO A    -4                                                      
REMARK 465     ARG A    -3                                                      
REMARK 465     GLY A    -2                                                      
REMARK 465     SER A    -1                                                      
REMARK 465     HIS A     0                                                      
REMARK 465     MET A     1                                                      
REMARK 465     SER A     2                                                      
REMARK 465     ASP A     3                                                      
REMARK 465     ASN A     4                                                      
REMARK 465     ALA A     5                                                      
REMARK 465     GLN A     6                                                      
REMARK 465     LEU A     7                                                      
REMARK 465     THR A     8                                                      
REMARK 465     SER A   177                                                      
REMARK 465     THR A   178                                                      
REMARK 465     GLN A   179                                                      
REMARK 465     ALA A   180                                                      
REMARK 465     HIS A   181                                                      
REMARK 465     MET B   -19                                                      
REMARK 465     GLY B   -18                                                      
REMARK 465     SER B   -17                                                      
REMARK 465     SER B   -16                                                      
REMARK 465     HIS B   -15                                                      
REMARK 465     HIS B   -14                                                      
REMARK 465     HIS B   -13                                                      
REMARK 465     HIS B   -12                                                      
REMARK 465     HIS B   -11                                                      
REMARK 465     HIS B   -10                                                      
REMARK 465     SER B    -9                                                      
REMARK 465     SER B    -8                                                      
REMARK 465     GLY B    -7                                                      
REMARK 465     LEU B    -6                                                      
REMARK 465     VAL B    -5                                                      
REMARK 465     PRO B    -4                                                      
REMARK 465     ARG B    -3                                                      
REMARK 465     GLY B    -2                                                      
REMARK 465     SER B    -1                                                      
REMARK 465     HIS B     0                                                      
REMARK 465     MET B     1                                                      
REMARK 465     SER B     2                                                      
REMARK 465     ASP B     3                                                      
REMARK 465     ASN B     4                                                      
REMARK 465     ALA B     5                                                      
REMARK 465     GLN B     6                                                      
REMARK 465     LEU B     7                                                      
REMARK 465     THR B     8                                                      
REMARK 465     VAL B   215                                                      
REMARK 465      DT D     1                                                      
REMARK 465     MET E   -19                                                      
REMARK 465     GLY E   -18                                                      
REMARK 465     SER E   -17                                                      
REMARK 465     SER E   -16                                                      
REMARK 465     HIS E   -15                                                      
REMARK 465     HIS E   -14                                                      
REMARK 465     HIS E   -13                                                      
REMARK 465     HIS E   -12                                                      
REMARK 465     HIS E   -11                                                      
REMARK 465     HIS E   -10                                                      
REMARK 465     SER E    -9                                                      
REMARK 465     SER E    -8                                                      
REMARK 465     GLY E    -7                                                      
REMARK 465     LEU E    -6                                                      
REMARK 465     VAL E    -5                                                      
REMARK 465     PRO E    -4                                                      
REMARK 465     ARG E    -3                                                      
REMARK 465     GLY E    -2                                                      
REMARK 465     SER E    -1                                                      
REMARK 465     HIS E     0                                                      
REMARK 465     MET E     1                                                      
REMARK 465     SER E     2                                                      
REMARK 465     ASP E     3                                                      
REMARK 465     ASN E     4                                                      
REMARK 465     ALA E     5                                                      
REMARK 465     GLN E     6                                                      
REMARK 465     LEU E     7                                                      
REMARK 465     THR E     8                                                      
REMARK 465     VAL E   215                                                      
REMARK 465     MET F   -19                                                      
REMARK 465     GLY F   -18                                                      
REMARK 465     SER F   -17                                                      
REMARK 465     SER F   -16                                                      
REMARK 465     HIS F   -15                                                      
REMARK 465     HIS F   -14                                                      
REMARK 465     HIS F   -13                                                      
REMARK 465     HIS F   -12                                                      
REMARK 465     HIS F   -11                                                      
REMARK 465     HIS F   -10                                                      
REMARK 465     SER F    -9                                                      
REMARK 465     SER F    -8                                                      
REMARK 465     GLY F    -7                                                      
REMARK 465     LEU F    -6                                                      
REMARK 465     VAL F    -5                                                      
REMARK 465     PRO F    -4                                                      
REMARK 465     ARG F    -3                                                      
REMARK 465     GLY F    -2                                                      
REMARK 465     SER F    -1                                                      
REMARK 465     HIS F     0                                                      
REMARK 465     MET F     1                                                      
REMARK 465     SER F     2                                                      
REMARK 465     ASP F     3                                                      
REMARK 465     ASN F     4                                                      
REMARK 465     ALA F     5                                                      
REMARK 465     GLN F     6                                                      
REMARK 465     LEU F     7                                                      
REMARK 465     THR F     8                                                      
REMARK 465     SER F   177                                                      
REMARK 465     THR F   178                                                      
REMARK 465     GLN F   179                                                      
REMARK 465     ALA F   180                                                      
REMARK 465     HIS F   181                                                      
REMARK 465      DT G     1                                                      
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES                                       
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)              
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3                                     
REMARK 500     DT C   1   O4' -  C1' -  N1  ANGL. DEV. =   2.3 DEGREES          
REMARK 500     DA C   5   O4' -  C1' -  N9  ANGL. DEV. =   1.9 DEGREES          
REMARK 500     DA D   5   O4' -  C1' -  N9  ANGL. DEV. =   2.7 DEGREES          
REMARK 500     DA D   6   O4' -  C1' -  N9  ANGL. DEV. =   1.9 DEGREES          
REMARK 500     DA G   5   O4' -  C1' -  N9  ANGL. DEV. =   3.0 DEGREES          
REMARK 500     DA G   6   O4' -  C1' -  N9  ANGL. DEV. =   1.9 DEGREES          
REMARK 500     DA H   5   O4' -  C1' -  N9  ANGL. DEV. =   2.4 DEGREES          
REMARK 500     DA H   6   O4' -  C1' -  N9  ANGL. DEV. =   2.9 DEGREES          
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    ASP A  55      -69.92   -108.60                                   
REMARK 500    ARG A 114      171.35    176.03                                   
REMARK 500    PHE A 146       -7.02   -141.93                                   
REMARK 500    GLU A 213       55.22   -118.39                                   
REMARK 500    ASP B  55      -93.20   -116.01                                   
REMARK 500    PRO B  82       -6.32    -58.22                                   
REMARK 500    PHE B 146       -9.45   -151.82                                   
REMARK 500    ASP E  55      -87.29   -114.43                                   
REMARK 500    PHE E 146      -19.14   -146.04                                   
REMARK 500    ASP F  55      -82.94   -112.76                                   
REMARK 500    ASP F  86       32.15    -97.28                                   
REMARK 500    PHE F 146      -20.52   -147.08                                   
REMARK 500    PHE F 175      119.15   -160.23                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 3NGY   RELATED DB: PDB                                   
REMARK 900 RNASE T (E92G MUTANT)                                                
REMARK 900 RELATED ID: 3NGZ   RELATED DB: PDB                                   
REMARK 900 RNASE T IN COMPLEX WITH A NON-PREFERRED SSDNA (GC) WITH ONE MG IN    
REMARK 900 THE ACTIVE SITE                                                      
REMARK 900 RELATED ID: 3NH0   RELATED DB: PDB                                   
REMARK 900 RNASE T IN COMPLEX WITH A NON-PREFERRED SSDNA (AAC)                  
REMARK 900 RELATED ID: 3NH1   RELATED DB: PDB                                   
REMARK 900 RNASE T IN COMPLEX WITH A PREFERRED SSDNA (TAGG) WITH TWO MG IN THE  
REMARK 900 ACTIVE SITE                                                          
DBREF  3NH2 A    1   215  UNP    P30014   RNT_ECOLI        1    215             
DBREF  3NH2 B    1   215  UNP    P30014   RNT_ECOLI        1    215             
DBREF  3NH2 C    1     7  PDB    3NH2     3NH2             1      7             
DBREF  3NH2 D    1     7  PDB    3NH2     3NH2             1      7             
DBREF  3NH2 E    1   215  UNP    P30014   RNT_ECOLI        1    215             
DBREF  3NH2 F    1   215  UNP    P30014   RNT_ECOLI        1    215             
DBREF  3NH2 G    1     7  PDB    3NH2     3NH2             1      7             
DBREF  3NH2 H    1     7  PDB    3NH2     3NH2             1      7             
SEQADV 3NH2 MET A  -19  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 GLY A  -18  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 SER A  -17  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 SER A  -16  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 HIS A  -15  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 HIS A  -14  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 HIS A  -13  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 HIS A  -12  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 HIS A  -11  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 HIS A  -10  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 SER A   -9  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 SER A   -8  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 GLY A   -7  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 LEU A   -6  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 VAL A   -5  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 PRO A   -4  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 ARG A   -3  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 GLY A   -2  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 SER A   -1  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 HIS A    0  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 MET B  -19  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 GLY B  -18  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 SER B  -17  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 SER B  -16  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 HIS B  -15  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 HIS B  -14  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 HIS B  -13  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 HIS B  -12  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 HIS B  -11  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 HIS B  -10  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 SER B   -9  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 SER B   -8  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 GLY B   -7  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 LEU B   -6  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 VAL B   -5  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 PRO B   -4  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 ARG B   -3  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 GLY B   -2  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 SER B   -1  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 HIS B    0  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 MET E  -19  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 GLY E  -18  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 SER E  -17  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 SER E  -16  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 HIS E  -15  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 HIS E  -14  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 HIS E  -13  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 HIS E  -12  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 HIS E  -11  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 HIS E  -10  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 SER E   -9  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 SER E   -8  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 GLY E   -7  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 LEU E   -6  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 VAL E   -5  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 PRO E   -4  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 ARG E   -3  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 GLY E   -2  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 SER E   -1  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 HIS E    0  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 MET F  -19  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 GLY F  -18  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 SER F  -17  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 SER F  -16  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 HIS F  -15  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 HIS F  -14  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 HIS F  -13  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 HIS F  -12  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 HIS F  -11  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 HIS F  -10  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 SER F   -9  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 SER F   -8  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 GLY F   -7  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 LEU F   -6  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 VAL F   -5  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 PRO F   -4  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 ARG F   -3  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 GLY F   -2  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 SER F   -1  UNP  P30014              EXPRESSION TAG                 
SEQADV 3NH2 HIS F    0  UNP  P30014              EXPRESSION TAG                 
SEQRES   1 A  235  MET GLY SER SER HIS HIS HIS HIS HIS HIS SER SER GLY          
SEQRES   2 A  235  LEU VAL PRO ARG GLY SER HIS MET SER ASP ASN ALA GLN          
SEQRES   3 A  235  LEU THR GLY LEU CYS ASP ARG PHE ARG GLY PHE TYR PRO          
SEQRES   4 A  235  VAL VAL ILE ASP VAL GLU THR ALA GLY PHE ASN ALA LYS          
SEQRES   5 A  235  THR ASP ALA LEU LEU GLU ILE ALA ALA ILE THR LEU LYS          
SEQRES   6 A  235  MET ASP GLU GLN GLY TRP LEU MET PRO ASP THR THR LEU          
SEQRES   7 A  235  HIS PHE HIS VAL GLU PRO PHE VAL GLY ALA ASN LEU GLN          
SEQRES   8 A  235  PRO GLU ALA LEU ALA PHE ASN GLY ILE ASP PRO ASN ASP          
SEQRES   9 A  235  PRO ASP ARG GLY ALA VAL SER GLU TYR GLU ALA LEU HIS          
SEQRES  10 A  235  GLU ILE PHE LYS VAL VAL ARG LYS GLY ILE LYS ALA SER          
SEQRES  11 A  235  GLY CYS ASN ARG ALA ILE MET VAL ALA HIS ASN ALA ASN          
SEQRES  12 A  235  PHE ASP HIS SER PHE MET MET ALA ALA ALA GLU ARG ALA          
SEQRES  13 A  235  SER LEU LYS ARG ASN PRO PHE HIS PRO PHE ALA THR PHE          
SEQRES  14 A  235  ASP THR ALA ALA LEU ALA GLY LEU ALA LEU GLY GLN THR          
SEQRES  15 A  235  VAL LEU SER LYS ALA CYS GLN THR ALA GLY MET ASP PHE          
SEQRES  16 A  235  ASP SER THR GLN ALA HIS SER ALA LEU TYR ASP THR GLU          
SEQRES  17 A  235  ARG THR ALA VAL LEU PHE CYS GLU ILE VAL ASN ARG TRP          
SEQRES  18 A  235  LYS ARG LEU GLY GLY TRP PRO LEU SER ALA ALA GLU GLU          
SEQRES  19 A  235  VAL                                                          
SEQRES   1 B  235  MET GLY SER SER HIS HIS HIS HIS HIS HIS SER SER GLY          
SEQRES   2 B  235  LEU VAL PRO ARG GLY SER HIS MET SER ASP ASN ALA GLN          
SEQRES   3 B  235  LEU THR GLY LEU CYS ASP ARG PHE ARG GLY PHE TYR PRO          
SEQRES   4 B  235  VAL VAL ILE ASP VAL GLU THR ALA GLY PHE ASN ALA LYS          
SEQRES   5 B  235  THR ASP ALA LEU LEU GLU ILE ALA ALA ILE THR LEU LYS          
SEQRES   6 B  235  MET ASP GLU GLN GLY TRP LEU MET PRO ASP THR THR LEU          
SEQRES   7 B  235  HIS PHE HIS VAL GLU PRO PHE VAL GLY ALA ASN LEU GLN          
SEQRES   8 B  235  PRO GLU ALA LEU ALA PHE ASN GLY ILE ASP PRO ASN ASP          
SEQRES   9 B  235  PRO ASP ARG GLY ALA VAL SER GLU TYR GLU ALA LEU HIS          
SEQRES  10 B  235  GLU ILE PHE LYS VAL VAL ARG LYS GLY ILE LYS ALA SER          
SEQRES  11 B  235  GLY CYS ASN ARG ALA ILE MET VAL ALA HIS ASN ALA ASN          
SEQRES  12 B  235  PHE ASP HIS SER PHE MET MET ALA ALA ALA GLU ARG ALA          
SEQRES  13 B  235  SER LEU LYS ARG ASN PRO PHE HIS PRO PHE ALA THR PHE          
SEQRES  14 B  235  ASP THR ALA ALA LEU ALA GLY LEU ALA LEU GLY GLN THR          
SEQRES  15 B  235  VAL LEU SER LYS ALA CYS GLN THR ALA GLY MET ASP PHE          
SEQRES  16 B  235  ASP SER THR GLN ALA HIS SER ALA LEU TYR ASP THR GLU          
SEQRES  17 B  235  ARG THR ALA VAL LEU PHE CYS GLU ILE VAL ASN ARG TRP          
SEQRES  18 B  235  LYS ARG LEU GLY GLY TRP PRO LEU SER ALA ALA GLU GLU          
SEQRES  19 B  235  VAL                                                          
SEQRES   1 C    7   DT  DT  DA  DC  DA  DA  DC                                  
SEQRES   1 D    7   DT  DT  DA  DC  DA  DA  DC                                  
SEQRES   1 E  235  MET GLY SER SER HIS HIS HIS HIS HIS HIS SER SER GLY          
SEQRES   2 E  235  LEU VAL PRO ARG GLY SER HIS MET SER ASP ASN ALA GLN          
SEQRES   3 E  235  LEU THR GLY LEU CYS ASP ARG PHE ARG GLY PHE TYR PRO          
SEQRES   4 E  235  VAL VAL ILE ASP VAL GLU THR ALA GLY PHE ASN ALA LYS          
SEQRES   5 E  235  THR ASP ALA LEU LEU GLU ILE ALA ALA ILE THR LEU LYS          
SEQRES   6 E  235  MET ASP GLU GLN GLY TRP LEU MET PRO ASP THR THR LEU          
SEQRES   7 E  235  HIS PHE HIS VAL GLU PRO PHE VAL GLY ALA ASN LEU GLN          
SEQRES   8 E  235  PRO GLU ALA LEU ALA PHE ASN GLY ILE ASP PRO ASN ASP          
SEQRES   9 E  235  PRO ASP ARG GLY ALA VAL SER GLU TYR GLU ALA LEU HIS          
SEQRES  10 E  235  GLU ILE PHE LYS VAL VAL ARG LYS GLY ILE LYS ALA SER          
SEQRES  11 E  235  GLY CYS ASN ARG ALA ILE MET VAL ALA HIS ASN ALA ASN          
SEQRES  12 E  235  PHE ASP HIS SER PHE MET MET ALA ALA ALA GLU ARG ALA          
SEQRES  13 E  235  SER LEU LYS ARG ASN PRO PHE HIS PRO PHE ALA THR PHE          
SEQRES  14 E  235  ASP THR ALA ALA LEU ALA GLY LEU ALA LEU GLY GLN THR          
SEQRES  15 E  235  VAL LEU SER LYS ALA CYS GLN THR ALA GLY MET ASP PHE          
SEQRES  16 E  235  ASP SER THR GLN ALA HIS SER ALA LEU TYR ASP THR GLU          
SEQRES  17 E  235  ARG THR ALA VAL LEU PHE CYS GLU ILE VAL ASN ARG TRP          
SEQRES  18 E  235  LYS ARG LEU GLY GLY TRP PRO LEU SER ALA ALA GLU GLU          
SEQRES  19 E  235  VAL                                                          
SEQRES   1 F  235  MET GLY SER SER HIS HIS HIS HIS HIS HIS SER SER GLY          
SEQRES   2 F  235  LEU VAL PRO ARG GLY SER HIS MET SER ASP ASN ALA GLN          
SEQRES   3 F  235  LEU THR GLY LEU CYS ASP ARG PHE ARG GLY PHE TYR PRO          
SEQRES   4 F  235  VAL VAL ILE ASP VAL GLU THR ALA GLY PHE ASN ALA LYS          
SEQRES   5 F  235  THR ASP ALA LEU LEU GLU ILE ALA ALA ILE THR LEU LYS          
SEQRES   6 F  235  MET ASP GLU GLN GLY TRP LEU MET PRO ASP THR THR LEU          
SEQRES   7 F  235  HIS PHE HIS VAL GLU PRO PHE VAL GLY ALA ASN LEU GLN          
SEQRES   8 F  235  PRO GLU ALA LEU ALA PHE ASN GLY ILE ASP PRO ASN ASP          
SEQRES   9 F  235  PRO ASP ARG GLY ALA VAL SER GLU TYR GLU ALA LEU HIS          
SEQRES  10 F  235  GLU ILE PHE LYS VAL VAL ARG LYS GLY ILE LYS ALA SER          
SEQRES  11 F  235  GLY CYS ASN ARG ALA ILE MET VAL ALA HIS ASN ALA ASN          
SEQRES  12 F  235  PHE ASP HIS SER PHE MET MET ALA ALA ALA GLU ARG ALA          
SEQRES  13 F  235  SER LEU LYS ARG ASN PRO PHE HIS PRO PHE ALA THR PHE          
SEQRES  14 F  235  ASP THR ALA ALA LEU ALA GLY LEU ALA LEU GLY GLN THR          
SEQRES  15 F  235  VAL LEU SER LYS ALA CYS GLN THR ALA GLY MET ASP PHE          
SEQRES  16 F  235  ASP SER THR GLN ALA HIS SER ALA LEU TYR ASP THR GLU          
SEQRES  17 F  235  ARG THR ALA VAL LEU PHE CYS GLU ILE VAL ASN ARG TRP          
SEQRES  18 F  235  LYS ARG LEU GLY GLY TRP PRO LEU SER ALA ALA GLU GLU          
SEQRES  19 F  235  VAL                                                          
SEQRES   1 G    7   DT  DT  DA  DC  DA  DA  DC                                  
SEQRES   1 H    7   DT  DT  DA  DC  DA  DA  DC                                  
FORMUL   9  HOH   *345(H2 O)                                                    
HELIX    1   1 GLY A    9  PHE A   14  1                                   6    
HELIX    2   2 GLN A   71  GLY A   79  1                                   9    
HELIX    3   3 SER A   91  GLY A  111  1                                  21    
HELIX    4   4 ALA A  122  ALA A  136  1                                  15    
HELIX    5   5 THR A  151  LEU A  159  1                                   9    
HELIX    6   6 VAL A  163  ALA A  171  1                                   9    
HELIX    7   7 SER A  182  LEU A  204  1                                  23    
HELIX    8   8 PRO A  208  GLU A  214  5                                   7    
HELIX    9   9 GLY B    9  PHE B   14  1                                   6    
HELIX   10  10 GLN B   71  GLY B   79  1                                   9    
HELIX   11  11 SER B   91  GLY B  111  1                                  21    
HELIX   12  12 ALA B  122  ALA B  136  1                                  15    
HELIX   13  13 THR B  151  GLY B  160  1                                  10    
HELIX   14  14 VAL B  163  ALA B  171  1                                   9    
HELIX   15  15 SER B  182  LEU B  204  1                                  23    
HELIX   16  16 SER B  210  GLU B  214  5                                   5    
HELIX   17  17 GLY E    9  PHE E   14  1                                   6    
HELIX   18  18 GLN E   71  GLY E   79  1                                   9    
HELIX   19  19 SER E   91  GLY E  111  1                                  21    
HELIX   20  20 ALA E  122  ALA E  136  1                                  15    
HELIX   21  21 THR E  151  LEU E  159  1                                   9    
HELIX   22  22 VAL E  163  ALA E  171  1                                   9    
HELIX   23  23 ASP E  176  ALA E  180  5                                   5    
HELIX   24  24 SER E  182  LEU E  204  1                                  23    
HELIX   25  25 GLY F    9  PHE F   14  1                                   6    
HELIX   26  26 GLN F   71  GLY F   79  1                                   9    
HELIX   27  27 ASP F   84  GLY F   88  5                                   5    
HELIX   28  28 SER F   91  GLY F  111  1                                  21    
HELIX   29  29 ALA F  122  ALA F  136  1                                  15    
HELIX   30  30 THR F  151  LEU F  159  1                                   9    
HELIX   31  31 VAL F  163  ALA F  171  1                                   9    
HELIX   32  32 SER F  182  LEU F  204  1                                  23    
HELIX   33  33 SER F  210  GLU F  214  5                                   5    
SHEET    1   A 5 LEU A  52  HIS A  61  0                                        
SHEET    2   A 5 LEU A  36  MET A  46 -1  N  THR A  43   O  ASP A  55           
SHEET    3   A 5 PHE A  17  THR A  26 -1  N  VAL A  21   O  ILE A  42           
SHEET    4   A 5 ARG A 114  ALA A 119  1  O  ARG A 114   N  TYR A  18           
SHEET    5   A 5 PHE A 143  ASP A 150  1  O  PHE A 149   N  MET A 117           
SHEET    1   B 5 LEU B  52  HIS B  61  0                                        
SHEET    2   B 5 LEU B  36  MET B  46 -1  N  THR B  43   O  ASP B  55           
SHEET    3   B 5 PHE B  17  THR B  26 -1  N  VAL B  21   O  ILE B  42           
SHEET    4   B 5 ARG B 114  ALA B 119  1  O  ARG B 114   N  TYR B  18           
SHEET    5   B 5 PHE B 143  ASP B 150  1  O  PHE B 149   N  MET B 117           
SHEET    1   C 5 LEU E  52  HIS E  61  0                                        
SHEET    2   C 5 LEU E  36  MET E  46 -1  N  THR E  43   O  ASP E  55           
SHEET    3   C 5 PHE E  17  THR E  26 -1  N  GLU E  25   O  LEU E  37           
SHEET    4   C 5 ARG E 114  ALA E 119  1  O  ARG E 114   N  TYR E  18           
SHEET    5   C 5 PHE E 143  ASP E 150  1  O  PHE E 149   N  MET E 117           
SHEET    1   D 5 LEU F  52  HIS F  61  0                                        
SHEET    2   D 5 LEU F  36  MET F  46 -1  N  THR F  43   O  ASP F  55           
SHEET    3   D 5 PHE F  17  THR F  26 -1  N  GLU F  25   O  LEU F  37           
SHEET    4   D 5 ARG F 114  ALA F 119  1  O  ARG F 114   N  TYR F  18           
SHEET    5   D 5 PHE F 143  ASP F 150  1  O  PHE F 149   N  MET F 117           
SSBOND   1 CYS A   11    CYS A  112                          1555   1555  2.01  
SSBOND   2 CYS E   11    CYS E  112                          1555   1555  2.03  
SSBOND   3 CYS F   11    CYS F  112                          1555   1555  2.03  
CISPEP   1 TRP A  207    PRO A  208          0        -2.67                     
CISPEP   2 TRP B  207    PRO B  208          0        -4.16                     
CISPEP   3 TRP E  207    PRO E  208          0        -3.82                     
CISPEP   4 TRP F  207    PRO F  208          0        -2.95                     
CISPEP   5 GLU F  214    VAL F  215          0         1.66                     
CRYST1   60.379   62.560   62.657  82.77  82.92  66.13 P 1           4          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.016562 -0.007329 -0.001448        0.00000                         
SCALE2      0.000000  0.017480 -0.001470        0.00000                         
SCALE3      0.000000  0.000000  0.016139        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
DBGET integrated database retrieval system