HEADER VIRAL PROTEIN 27-SEP-10 3P0A
TITLE X-RAY STRUCTURE OF PENTAMERIC HIV-1 CA
COMPND MOL_ID: 1;
COMPND 2 MOLECULE: HIV-1 CA;
COMPND 3 CHAIN: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T;
COMPND 4 FRAGMENT: UNP RESIDUES 133-363;
COMPND 5 ENGINEERED: YES;
COMPND 6 MUTATION: YES
SOURCE MOL_ID: 1;
SOURCE 2 ORGANISM_SCIENTIFIC: HUMAN IMMUNODEFICIENCY VIRUS 1;
SOURCE 3 ORGANISM_TAXID: 11676;
SOURCE 4 STRAIN: NL4-3;
SOURCE 5 GENE: CA, GAG;
SOURCE 6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
SOURCE 7 EXPRESSION_SYSTEM_TAXID: 562;
SOURCE 8 EXPRESSION_SYSTEM_STRAIN: BL21(DE3);
SOURCE 9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;
SOURCE 10 EXPRESSION_SYSTEM_PLASMID: PET11A
KEYWDS CAPSID PROTEIN, VIRAL PROTEIN
EXPDTA X-RAY DIFFRACTION
MDLTYP CA ATOMS ONLY, CHAIN A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P,
MDLTYP 2Q, R, S, T
AUTHOR O.PORNILLOS
REVDAT 3 06-SEP-23 3P0A 1 SEQADV
REVDAT 2 16-MAR-11 3P0A 1 JRNL
REVDAT 1 12-JAN-11 3P0A 0
JRNL AUTH O.PORNILLOS,B.K.GANSER-PORNILLOS,M.YEAGER
JRNL TITL ATOMIC-LEVEL MODELLING OF THE HIV CAPSID.
JRNL REF NATURE V. 469 424 2011
JRNL REFN ISSN 0028-0836
JRNL PMID 21248851
JRNL DOI 10.1038/NATURE09640
REMARK 2
REMARK 2 RESOLUTION. 5.95 ANGSTROMS.
REMARK 3
REMARK 3 REFINEMENT.
REMARK 3 PROGRAM : PHENIX (PHENIX.REFINE: 1.6.1_357)
REMARK 3 AUTHORS : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN
REMARK 3 : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,
REMARK 3 : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,
REMARK 3 : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,
REMARK 3 : REETAL PAI,RANDY READ,JANE RICHARDSON,
REMARK 3 : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,
REMARK 3 : NICHOLAS SAUTER,JACOB SMITH,LAURENT
REMARK 3 : STORONI,TOM TERWILLIGER,PETER ZWART
REMARK 3
REMARK 3 REFINEMENT TARGET : ML
REMARK 3
REMARK 3 DATA USED IN REFINEMENT.
REMARK 3 RESOLUTION RANGE HIGH (ANGSTROMS) : 5.95
REMARK 3 RESOLUTION RANGE LOW (ANGSTROMS) : 45.56
REMARK 3 MIN(FOBS/SIGMA_FOBS) : 1.970
REMARK 3 COMPLETENESS FOR RANGE (%) : 97.9
REMARK 3 NUMBER OF REFLECTIONS : 14169
REMARK 3
REMARK 3 FIT TO DATA USED IN REFINEMENT.
REMARK 3 R VALUE (WORKING + TEST SET) : 0.299
REMARK 3 R VALUE (WORKING SET) : 0.297
REMARK 3 FREE R VALUE : 0.316
REMARK 3 FREE R VALUE TEST SET SIZE (%) : 9.990
REMARK 3 FREE R VALUE TEST SET COUNT : 1416
REMARK 3
REMARK 3 FIT TO DATA USED IN REFINEMENT (IN BINS).
REMARK 3 BIN RESOLUTION RANGE COMPL. NWORK NFREE RWORK RFREE
REMARK 3 1 45.5637 - 12.7436 0.98 1273 139 0.3201 0.3565
REMARK 3 2 12.7436 - 10.1518 0.98 1292 141 0.2333 0.2615
REMARK 3 3 10.1518 - 8.8793 1.00 1287 142 0.2329 0.2740
REMARK 3 4 8.8793 - 8.0724 1.00 1307 142 0.2805 0.2777
REMARK 3 5 8.0724 - 7.4965 1.00 1306 142 0.3182 0.3045
REMARK 3 6 7.4965 - 7.0562 1.00 1303 142 0.3223 0.3361
REMARK 3 7 7.0562 - 6.7040 1.00 1311 142 0.3613 0.3696
REMARK 3 8 6.7040 - 6.4130 1.00 1289 142 0.4007 0.3467
REMARK 3 9 6.4130 - 6.1667 0.99 1265 142 0.3904 0.4135
REMARK 3 10 6.1667 - 5.9540 0.86 1120 142 0.3934 0.3806
REMARK 3
REMARK 3 BULK SOLVENT MODELLING.
REMARK 3 METHOD USED : FLAT BULK SOLVENT MODEL
REMARK 3 SOLVENT RADIUS : 1.11
REMARK 3 SHRINKAGE RADIUS : 0.90
REMARK 3 K_SOL : 0.31
REMARK 3 B_SOL : 20.00
REMARK 3
REMARK 3 ERROR ESTIMATES.
REMARK 3 COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED) : 1.080
REMARK 3 PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 36.410
REMARK 3
REMARK 3 B VALUES.
REMARK 3 FROM WILSON PLOT (A**2) : NULL
REMARK 3 MEAN B VALUE (OVERALL, A**2) : NULL
REMARK 3 OVERALL ANISOTROPIC B VALUE.
REMARK 3 B11 (A**2) : 279.73810
REMARK 3 B22 (A**2) : 159.85560
REMARK 3 B33 (A**2) : 125.01040
REMARK 3 B12 (A**2) : -17.68020
REMARK 3 B13 (A**2) : 17.34710
REMARK 3 B23 (A**2) : -9.22810
REMARK 3
REMARK 3 TWINNING INFORMATION.
REMARK 3 FRACTION: NULL
REMARK 3 OPERATOR: NULL
REMARK 3
REMARK 3 DEVIATIONS FROM IDEAL VALUES.
REMARK 3 RMSD COUNT
REMARK 3 BOND : 0.029 30704
REMARK 3 ANGLE : 1.827 41808
REMARK 3 CHIRALITY : 0.099 4856
REMARK 3 PLANARITY : 0.010 5368
REMARK 3 DIHEDRAL : 15.564 11256
REMARK 3
REMARK 3 TLS DETAILS
REMARK 3 NUMBER OF TLS GROUPS : NULL
REMARK 3
REMARK 3 NCS DETAILS
REMARK 3 NUMBER OF NCS GROUPS : NULL
REMARK 3
REMARK 3 OTHER REFINEMENT REMARKS: THE COORDINATES CONTAIN ONLY CALPHA
REMARK 3 ATOMS
REMARK 4
REMARK 4 3P0A COMPLIES WITH FORMAT V. 3.30, 13-JUL-11
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 04-OCT-10.
REMARK 100 THE DEPOSITION ID IS D_1000061795.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200 EXPERIMENT TYPE : X-RAY DIFFRACTION
REMARK 200 DATE OF DATA COLLECTION : 08-MAR-10
REMARK 200 TEMPERATURE (KELVIN) : 100
REMARK 200 PH : 7-8
REMARK 200 NUMBER OF CRYSTALS USED : NULL
REMARK 200
REMARK 200 SYNCHROTRON (Y/N) : Y
REMARK 200 RADIATION SOURCE : APS
REMARK 200 BEAMLINE : 22-ID
REMARK 200 X-RAY GENERATOR MODEL : NULL
REMARK 200 MONOCHROMATIC OR LAUE (M/L) : M
REMARK 200 WAVELENGTH OR RANGE (A) : 1.000
REMARK 200 MONOCHROMATOR : NULL
REMARK 200 OPTICS : NULL
REMARK 200
REMARK 200 DETECTOR TYPE : CCD
REMARK 200 DETECTOR MANUFACTURER : MARMOSAIC 300 MM CCD
REMARK 200 INTENSITY-INTEGRATION SOFTWARE : HKL-2000
REMARK 200 DATA SCALING SOFTWARE : HKL-2000
REMARK 200
REMARK 200 NUMBER OF UNIQUE REFLECTIONS : 14213
REMARK 200 RESOLUTION RANGE HIGH (A) : 5.950
REMARK 200 RESOLUTION RANGE LOW (A) : 50.000
REMARK 200 REJECTION CRITERIA (SIGMA(I)) : 2.000
REMARK 200
REMARK 200 OVERALL.
REMARK 200 COMPLETENESS FOR RANGE (%) : 99.4
REMARK 200 DATA REDUNDANCY : NULL
REMARK 200 R MERGE (I) : NULL
REMARK 200 R SYM (I) : NULL
REMARK 200 <I/SIGMA(I)> FOR THE DATA SET : NULL
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200 HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 5.95
REMARK 200 HIGHEST RESOLUTION SHELL, RANGE LOW (A) : 6.32
REMARK 200 COMPLETENESS FOR SHELL (%) : 98.6
REMARK 200 DATA REDUNDANCY IN SHELL : 3.90
REMARK 200 R MERGE FOR SHELL (I) : NULL
REMARK 200 R SYM FOR SHELL (I) : 0.57900
REMARK 200 <I/SIGMA(I)> FOR SHELL : 2.300
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: MOLREP
REMARK 200 STARTING MODEL: PDB ENTRY 3P05
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS (%): 57.26
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.88
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: 30-32% PEG 2000 MME, 100 MM TRIS, 0.4
REMARK 280 M SODIUM IODIDE, PH 7-8, VAPOR DIFFUSION, SITTING DROP,
REMARK 280 TEMPERATURE 293K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 1
REMARK 290
REMARK 290 SYMOP SYMMETRY
REMARK 290 NNNMMM OPERATOR
REMARK 290 1555 X,Y,Z
REMARK 290
REMARK 290 WHERE NNN -> OPERATOR NUMBER
REMARK 290 MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290 SMTRY1 1 1.000000 0.000000 0.000000 0.00000
REMARK 290 SMTRY2 1 0.000000 1.000000 0.000000 0.00000
REMARK 290 SMTRY3 1 0.000000 0.000000 1.000000 0.00000
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1, 2, 3, 4
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW. BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: PENTAMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, C, D, E
REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000
REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000
REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 2
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: PENTAMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: F, G, H, I, J
REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000
REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000
REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 3
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: PENTAMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: K, L, M, N, O
REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000
REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000
REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000
REMARK 350
REMARK 350 BIOMOLECULE: 4
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: PENTAMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: P, Q, R, S, T
REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000
REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000
REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000
REMARK 400
REMARK 400 COMPOUND
REMARK 400 THE REFINEMENT WAS LIMITED TO RIGID-BODY PLACEMENT OF THE MONOMERS.
REMARK 400 THE PROTEIN IS COMPOSED OF TWO DOMAINS, WHICH WERE TREATED AS
REMARK 400 SEPARATE RIGID BODIES DURING REFINEMENT. THE TWO DOMAINS WERE
REMARK 400 ALLOWED TO MOVE INDEPENDENTLY AND THAT IS WHY THE CONNECTIVITY
REMARK 400 BETWEEN THEM IS NOT WELL-PRESERVED.
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465 M RES C SSSEQI
REMARK 465 VAL A 3
REMARK 465 GLN A 4
REMARK 465 ASN A 5
REMARK 465 LEU A 6
REMARK 465 GLN A 7
REMARK 465 GLY A 8
REMARK 465 GLN A 9
REMARK 465 MET A 10
REMARK 465 VAL A 11
REMARK 465 VAL A 86
REMARK 465 HIS A 87
REMARK 465 ALA A 88
REMARK 465 GLY A 89
REMARK 465 PRO A 90
REMARK 465 ILE A 91
REMARK 465 ALA A 92
REMARK 465 PRO A 93
REMARK 465 GLY A 94
REMARK 465 GLN A 95
REMARK 465 MET A 96
REMARK 465 ARG A 97
REMARK 465 GLU A 98
REMARK 465 GLY A 220
REMARK 465 VAL A 221
REMARK 465 GLY A 222
REMARK 465 GLY A 223
REMARK 465 PRO A 224
REMARK 465 GLY A 225
REMARK 465 HIS A 226
REMARK 465 LYS A 227
REMARK 465 ALA A 228
REMARK 465 ARG A 229
REMARK 465 VAL A 230
REMARK 465 LEU A 231
REMARK 465 VAL B 3
REMARK 465 GLN B 4
REMARK 465 ASN B 5
REMARK 465 LEU B 6
REMARK 465 GLN B 7
REMARK 465 GLY B 8
REMARK 465 GLN B 9
REMARK 465 MET B 10
REMARK 465 VAL B 86
REMARK 465 HIS B 87
REMARK 465 ALA B 88
REMARK 465 GLY B 89
REMARK 465 PRO B 90
REMARK 465 ILE B 91
REMARK 465 ALA B 92
REMARK 465 PRO B 93
REMARK 465 GLY B 94
REMARK 465 GLN B 95
REMARK 465 MET B 96
REMARK 465 ARG B 97
REMARK 465 GLU B 98
REMARK 465 GLY B 220
REMARK 465 VAL B 221
REMARK 465 GLY B 222
REMARK 465 GLY B 223
REMARK 465 PRO B 224
REMARK 465 GLY B 225
REMARK 465 HIS B 226
REMARK 465 LYS B 227
REMARK 465 ALA B 228
REMARK 465 ARG B 229
REMARK 465 VAL B 230
REMARK 465 LEU B 231
REMARK 465 VAL C 3
REMARK 465 GLN C 4
REMARK 465 ASN C 5
REMARK 465 LEU C 6
REMARK 465 GLN C 7
REMARK 465 GLY C 8
REMARK 465 GLN C 9
REMARK 465 MET C 10
REMARK 465 VAL C 86
REMARK 465 HIS C 87
REMARK 465 ALA C 88
REMARK 465 GLY C 89
REMARK 465 PRO C 90
REMARK 465 ILE C 91
REMARK 465 ALA C 92
REMARK 465 PRO C 93
REMARK 465 GLY C 94
REMARK 465 GLN C 95
REMARK 465 MET C 96
REMARK 465 ARG C 97
REMARK 465 GLU C 98
REMARK 465 GLY C 220
REMARK 465 VAL C 221
REMARK 465 GLY C 222
REMARK 465 GLY C 223
REMARK 465 PRO C 224
REMARK 465 GLY C 225
REMARK 465 HIS C 226
REMARK 465 LYS C 227
REMARK 465 ALA C 228
REMARK 465 ARG C 229
REMARK 465 VAL C 230
REMARK 465 LEU C 231
REMARK 465 VAL D 3
REMARK 465 GLN D 4
REMARK 465 ASN D 5
REMARK 465 LEU D 6
REMARK 465 GLN D 7
REMARK 465 GLY D 8
REMARK 465 GLN D 9
REMARK 465 MET D 10
REMARK 465 VAL D 86
REMARK 465 HIS D 87
REMARK 465 ALA D 88
REMARK 465 GLY D 89
REMARK 465 PRO D 90
REMARK 465 ILE D 91
REMARK 465 ALA D 92
REMARK 465 PRO D 93
REMARK 465 GLY D 94
REMARK 465 GLN D 95
REMARK 465 MET D 96
REMARK 465 ARG D 97
REMARK 465 GLU D 98
REMARK 465 GLY D 220
REMARK 465 VAL D 221
REMARK 465 GLY D 222
REMARK 465 GLY D 223
REMARK 465 PRO D 224
REMARK 465 GLY D 225
REMARK 465 HIS D 226
REMARK 465 LYS D 227
REMARK 465 ALA D 228
REMARK 465 ARG D 229
REMARK 465 VAL D 230
REMARK 465 LEU D 231
REMARK 465 VAL E 3
REMARK 465 GLN E 4
REMARK 465 ASN E 5
REMARK 465 LEU E 6
REMARK 465 GLN E 7
REMARK 465 GLY E 8
REMARK 465 GLN E 9
REMARK 465 MET E 10
REMARK 465 VAL E 11
REMARK 465 VAL E 86
REMARK 465 HIS E 87
REMARK 465 ALA E 88
REMARK 465 GLY E 89
REMARK 465 PRO E 90
REMARK 465 ILE E 91
REMARK 465 ALA E 92
REMARK 465 PRO E 93
REMARK 465 GLY E 94
REMARK 465 GLN E 95
REMARK 465 MET E 96
REMARK 465 ARG E 97
REMARK 465 GLU E 98
REMARK 465 GLY E 220
REMARK 465 VAL E 221
REMARK 465 GLY E 222
REMARK 465 GLY E 223
REMARK 465 PRO E 224
REMARK 465 GLY E 225
REMARK 465 HIS E 226
REMARK 465 LYS E 227
REMARK 465 ALA E 228
REMARK 465 ARG E 229
REMARK 465 VAL E 230
REMARK 465 LEU E 231
REMARK 465 VAL F 3
REMARK 465 GLN F 4
REMARK 465 ASN F 5
REMARK 465 LEU F 6
REMARK 465 GLN F 7
REMARK 465 GLY F 8
REMARK 465 GLN F 9
REMARK 465 MET F 10
REMARK 465 VAL F 11
REMARK 465 VAL F 86
REMARK 465 HIS F 87
REMARK 465 ALA F 88
REMARK 465 GLY F 89
REMARK 465 PRO F 90
REMARK 465 ILE F 91
REMARK 465 ALA F 92
REMARK 465 PRO F 93
REMARK 465 GLY F 94
REMARK 465 GLN F 95
REMARK 465 MET F 96
REMARK 465 ARG F 97
REMARK 465 GLU F 98
REMARK 465 GLY F 220
REMARK 465 VAL F 221
REMARK 465 GLY F 222
REMARK 465 GLY F 223
REMARK 465 PRO F 224
REMARK 465 GLY F 225
REMARK 465 HIS F 226
REMARK 465 LYS F 227
REMARK 465 ALA F 228
REMARK 465 ARG F 229
REMARK 465 VAL F 230
REMARK 465 LEU F 231
REMARK 465 VAL G 3
REMARK 465 GLN G 4
REMARK 465 ASN G 5
REMARK 465 LEU G 6
REMARK 465 GLN G 7
REMARK 465 GLY G 8
REMARK 465 GLN G 9
REMARK 465 MET G 10
REMARK 465 VAL G 86
REMARK 465 HIS G 87
REMARK 465 ALA G 88
REMARK 465 GLY G 89
REMARK 465 PRO G 90
REMARK 465 ILE G 91
REMARK 465 ALA G 92
REMARK 465 PRO G 93
REMARK 465 GLY G 94
REMARK 465 GLN G 95
REMARK 465 MET G 96
REMARK 465 ARG G 97
REMARK 465 GLU G 98
REMARK 465 GLY G 220
REMARK 465 VAL G 221
REMARK 465 GLY G 222
REMARK 465 GLY G 223
REMARK 465 PRO G 224
REMARK 465 GLY G 225
REMARK 465 HIS G 226
REMARK 465 LYS G 227
REMARK 465 ALA G 228
REMARK 465 ARG G 229
REMARK 465 VAL G 230
REMARK 465 LEU G 231
REMARK 465 VAL H 3
REMARK 465 GLN H 4
REMARK 465 ASN H 5
REMARK 465 LEU H 6
REMARK 465 GLN H 7
REMARK 465 GLY H 8
REMARK 465 GLN H 9
REMARK 465 MET H 10
REMARK 465 VAL H 86
REMARK 465 HIS H 87
REMARK 465 ALA H 88
REMARK 465 GLY H 89
REMARK 465 PRO H 90
REMARK 465 ILE H 91
REMARK 465 ALA H 92
REMARK 465 PRO H 93
REMARK 465 GLY H 94
REMARK 465 GLN H 95
REMARK 465 MET H 96
REMARK 465 ARG H 97
REMARK 465 GLU H 98
REMARK 465 GLY H 220
REMARK 465 VAL H 221
REMARK 465 GLY H 222
REMARK 465 GLY H 223
REMARK 465 PRO H 224
REMARK 465 GLY H 225
REMARK 465 HIS H 226
REMARK 465 LYS H 227
REMARK 465 ALA H 228
REMARK 465 ARG H 229
REMARK 465 VAL H 230
REMARK 465 LEU H 231
REMARK 465 VAL I 3
REMARK 465 GLN I 4
REMARK 465 ASN I 5
REMARK 465 LEU I 6
REMARK 465 GLN I 7
REMARK 465 GLY I 8
REMARK 465 GLN I 9
REMARK 465 MET I 10
REMARK 465 VAL I 86
REMARK 465 HIS I 87
REMARK 465 ALA I 88
REMARK 465 GLY I 89
REMARK 465 PRO I 90
REMARK 465 ILE I 91
REMARK 465 ALA I 92
REMARK 465 PRO I 93
REMARK 465 GLY I 94
REMARK 465 GLN I 95
REMARK 465 MET I 96
REMARK 465 ARG I 97
REMARK 465 GLU I 98
REMARK 465 GLY I 220
REMARK 465 VAL I 221
REMARK 465 GLY I 222
REMARK 465 GLY I 223
REMARK 465 PRO I 224
REMARK 465 GLY I 225
REMARK 465 HIS I 226
REMARK 465 LYS I 227
REMARK 465 ALA I 228
REMARK 465 ARG I 229
REMARK 465 VAL I 230
REMARK 465 LEU I 231
REMARK 465 VAL J 3
REMARK 465 GLN J 4
REMARK 465 ASN J 5
REMARK 465 LEU J 6
REMARK 465 GLN J 7
REMARK 465 GLY J 8
REMARK 465 GLN J 9
REMARK 465 MET J 10
REMARK 465 VAL J 11
REMARK 465 VAL J 86
REMARK 465 HIS J 87
REMARK 465 ALA J 88
REMARK 465 GLY J 89
REMARK 465 PRO J 90
REMARK 465 ILE J 91
REMARK 465 ALA J 92
REMARK 465 PRO J 93
REMARK 465 GLY J 94
REMARK 465 GLN J 95
REMARK 465 MET J 96
REMARK 465 ARG J 97
REMARK 465 GLU J 98
REMARK 465 GLY J 220
REMARK 465 VAL J 221
REMARK 465 GLY J 222
REMARK 465 GLY J 223
REMARK 465 PRO J 224
REMARK 465 GLY J 225
REMARK 465 HIS J 226
REMARK 465 LYS J 227
REMARK 465 ALA J 228
REMARK 465 ARG J 229
REMARK 465 VAL J 230
REMARK 465 LEU J 231
REMARK 465 VAL K 3
REMARK 465 GLN K 4
REMARK 465 ASN K 5
REMARK 465 LEU K 6
REMARK 465 GLN K 7
REMARK 465 GLY K 8
REMARK 465 GLN K 9
REMARK 465 MET K 10
REMARK 465 VAL K 11
REMARK 465 VAL K 86
REMARK 465 HIS K 87
REMARK 465 ALA K 88
REMARK 465 GLY K 89
REMARK 465 PRO K 90
REMARK 465 ILE K 91
REMARK 465 ALA K 92
REMARK 465 PRO K 93
REMARK 465 GLY K 94
REMARK 465 GLN K 95
REMARK 465 MET K 96
REMARK 465 ARG K 97
REMARK 465 GLU K 98
REMARK 465 GLY K 220
REMARK 465 VAL K 221
REMARK 465 GLY K 222
REMARK 465 GLY K 223
REMARK 465 PRO K 224
REMARK 465 GLY K 225
REMARK 465 HIS K 226
REMARK 465 LYS K 227
REMARK 465 ALA K 228
REMARK 465 ARG K 229
REMARK 465 VAL K 230
REMARK 465 LEU K 231
REMARK 465 VAL L 3
REMARK 465 GLN L 4
REMARK 465 ASN L 5
REMARK 465 LEU L 6
REMARK 465 GLN L 7
REMARK 465 GLY L 8
REMARK 465 GLN L 9
REMARK 465 MET L 10
REMARK 465 VAL L 86
REMARK 465 HIS L 87
REMARK 465 ALA L 88
REMARK 465 GLY L 89
REMARK 465 PRO L 90
REMARK 465 ILE L 91
REMARK 465 ALA L 92
REMARK 465 PRO L 93
REMARK 465 GLY L 94
REMARK 465 GLN L 95
REMARK 465 MET L 96
REMARK 465 ARG L 97
REMARK 465 GLU L 98
REMARK 465 GLY L 220
REMARK 465 VAL L 221
REMARK 465 GLY L 222
REMARK 465 GLY L 223
REMARK 465 PRO L 224
REMARK 465 GLY L 225
REMARK 465 HIS L 226
REMARK 465 LYS L 227
REMARK 465 ALA L 228
REMARK 465 ARG L 229
REMARK 465 VAL L 230
REMARK 465 LEU L 231
REMARK 465 VAL M 3
REMARK 465 GLN M 4
REMARK 465 ASN M 5
REMARK 465 LEU M 6
REMARK 465 GLN M 7
REMARK 465 GLY M 8
REMARK 465 GLN M 9
REMARK 465 MET M 10
REMARK 465 VAL M 86
REMARK 465 HIS M 87
REMARK 465 ALA M 88
REMARK 465 GLY M 89
REMARK 465 PRO M 90
REMARK 465 ILE M 91
REMARK 465 ALA M 92
REMARK 465 PRO M 93
REMARK 465 GLY M 94
REMARK 465 GLN M 95
REMARK 465 MET M 96
REMARK 465 ARG M 97
REMARK 465 GLU M 98
REMARK 465 GLY M 220
REMARK 465 VAL M 221
REMARK 465 GLY M 222
REMARK 465 GLY M 223
REMARK 465 PRO M 224
REMARK 465 GLY M 225
REMARK 465 HIS M 226
REMARK 465 LYS M 227
REMARK 465 ALA M 228
REMARK 465 ARG M 229
REMARK 465 VAL M 230
REMARK 465 LEU M 231
REMARK 465 VAL N 3
REMARK 465 GLN N 4
REMARK 465 ASN N 5
REMARK 465 LEU N 6
REMARK 465 GLN N 7
REMARK 465 GLY N 8
REMARK 465 GLN N 9
REMARK 465 MET N 10
REMARK 465 VAL N 86
REMARK 465 HIS N 87
REMARK 465 ALA N 88
REMARK 465 GLY N 89
REMARK 465 PRO N 90
REMARK 465 ILE N 91
REMARK 465 ALA N 92
REMARK 465 PRO N 93
REMARK 465 GLY N 94
REMARK 465 GLN N 95
REMARK 465 MET N 96
REMARK 465 ARG N 97
REMARK 465 GLU N 98
REMARK 465 GLY N 220
REMARK 465 VAL N 221
REMARK 465 GLY N 222
REMARK 465 GLY N 223
REMARK 465 PRO N 224
REMARK 465 GLY N 225
REMARK 465 HIS N 226
REMARK 465 LYS N 227
REMARK 465 ALA N 228
REMARK 465 ARG N 229
REMARK 465 VAL N 230
REMARK 465 LEU N 231
REMARK 465 VAL O 3
REMARK 465 GLN O 4
REMARK 465 ASN O 5
REMARK 465 LEU O 6
REMARK 465 GLN O 7
REMARK 465 GLY O 8
REMARK 465 GLN O 9
REMARK 465 MET O 10
REMARK 465 VAL O 11
REMARK 465 VAL O 86
REMARK 465 HIS O 87
REMARK 465 ALA O 88
REMARK 465 GLY O 89
REMARK 465 PRO O 90
REMARK 465 ILE O 91
REMARK 465 ALA O 92
REMARK 465 PRO O 93
REMARK 465 GLY O 94
REMARK 465 GLN O 95
REMARK 465 MET O 96
REMARK 465 ARG O 97
REMARK 465 GLU O 98
REMARK 465 GLY O 220
REMARK 465 VAL O 221
REMARK 465 GLY O 222
REMARK 465 GLY O 223
REMARK 465 PRO O 224
REMARK 465 GLY O 225
REMARK 465 HIS O 226
REMARK 465 LYS O 227
REMARK 465 ALA O 228
REMARK 465 ARG O 229
REMARK 465 VAL O 230
REMARK 465 LEU O 231
REMARK 465 VAL P 3
REMARK 465 GLN P 4
REMARK 465 ASN P 5
REMARK 465 LEU P 6
REMARK 465 GLN P 7
REMARK 465 GLY P 8
REMARK 465 GLN P 9
REMARK 465 MET P 10
REMARK 465 VAL P 11
REMARK 465 VAL P 86
REMARK 465 HIS P 87
REMARK 465 ALA P 88
REMARK 465 GLY P 89
REMARK 465 PRO P 90
REMARK 465 ILE P 91
REMARK 465 ALA P 92
REMARK 465 PRO P 93
REMARK 465 GLY P 94
REMARK 465 GLN P 95
REMARK 465 MET P 96
REMARK 465 ARG P 97
REMARK 465 GLU P 98
REMARK 465 GLY P 220
REMARK 465 VAL P 221
REMARK 465 GLY P 222
REMARK 465 GLY P 223
REMARK 465 PRO P 224
REMARK 465 GLY P 225
REMARK 465 HIS P 226
REMARK 465 LYS P 227
REMARK 465 ALA P 228
REMARK 465 ARG P 229
REMARK 465 VAL P 230
REMARK 465 LEU P 231
REMARK 465 VAL Q 3
REMARK 465 GLN Q 4
REMARK 465 ASN Q 5
REMARK 465 LEU Q 6
REMARK 465 GLN Q 7
REMARK 465 GLY Q 8
REMARK 465 GLN Q 9
REMARK 465 MET Q 10
REMARK 465 VAL Q 86
REMARK 465 HIS Q 87
REMARK 465 ALA Q 88
REMARK 465 GLY Q 89
REMARK 465 PRO Q 90
REMARK 465 ILE Q 91
REMARK 465 ALA Q 92
REMARK 465 PRO Q 93
REMARK 465 GLY Q 94
REMARK 465 GLN Q 95
REMARK 465 MET Q 96
REMARK 465 ARG Q 97
REMARK 465 GLU Q 98
REMARK 465 GLY Q 220
REMARK 465 VAL Q 221
REMARK 465 GLY Q 222
REMARK 465 GLY Q 223
REMARK 465 PRO Q 224
REMARK 465 GLY Q 225
REMARK 465 HIS Q 226
REMARK 465 LYS Q 227
REMARK 465 ALA Q 228
REMARK 465 ARG Q 229
REMARK 465 VAL Q 230
REMARK 465 LEU Q 231
REMARK 465 VAL R 3
REMARK 465 GLN R 4
REMARK 465 ASN R 5
REMARK 465 LEU R 6
REMARK 465 GLN R 7
REMARK 465 GLY R 8
REMARK 465 GLN R 9
REMARK 465 MET R 10
REMARK 465 VAL R 86
REMARK 465 HIS R 87
REMARK 465 ALA R 88
REMARK 465 GLY R 89
REMARK 465 PRO R 90
REMARK 465 ILE R 91
REMARK 465 ALA R 92
REMARK 465 PRO R 93
REMARK 465 GLY R 94
REMARK 465 GLN R 95
REMARK 465 MET R 96
REMARK 465 ARG R 97
REMARK 465 GLU R 98
REMARK 465 GLY R 220
REMARK 465 VAL R 221
REMARK 465 GLY R 222
REMARK 465 GLY R 223
REMARK 465 PRO R 224
REMARK 465 GLY R 225
REMARK 465 HIS R 226
REMARK 465 LYS R 227
REMARK 465 ALA R 228
REMARK 465 ARG R 229
REMARK 465 VAL R 230
REMARK 465 LEU R 231
REMARK 465 VAL S 3
REMARK 465 GLN S 4
REMARK 465 ASN S 5
REMARK 465 LEU S 6
REMARK 465 GLN S 7
REMARK 465 GLY S 8
REMARK 465 GLN S 9
REMARK 465 MET S 10
REMARK 465 VAL S 86
REMARK 465 HIS S 87
REMARK 465 ALA S 88
REMARK 465 GLY S 89
REMARK 465 PRO S 90
REMARK 465 ILE S 91
REMARK 465 ALA S 92
REMARK 465 PRO S 93
REMARK 465 GLY S 94
REMARK 465 GLN S 95
REMARK 465 MET S 96
REMARK 465 ARG S 97
REMARK 465 GLU S 98
REMARK 465 GLY S 220
REMARK 465 VAL S 221
REMARK 465 GLY S 222
REMARK 465 GLY S 223
REMARK 465 PRO S 224
REMARK 465 GLY S 225
REMARK 465 HIS S 226
REMARK 465 LYS S 227
REMARK 465 ALA S 228
REMARK 465 ARG S 229
REMARK 465 VAL S 230
REMARK 465 LEU S 231
REMARK 465 VAL T 3
REMARK 465 GLN T 4
REMARK 465 ASN T 5
REMARK 465 LEU T 6
REMARK 465 GLN T 7
REMARK 465 GLY T 8
REMARK 465 GLN T 9
REMARK 465 MET T 10
REMARK 465 VAL T 11
REMARK 465 VAL T 86
REMARK 465 HIS T 87
REMARK 465 ALA T 88
REMARK 465 GLY T 89
REMARK 465 PRO T 90
REMARK 465 ILE T 91
REMARK 465 ALA T 92
REMARK 465 PRO T 93
REMARK 465 GLY T 94
REMARK 465 GLN T 95
REMARK 465 MET T 96
REMARK 465 ARG T 97
REMARK 465 GLU T 98
REMARK 465 GLY T 220
REMARK 465 VAL T 221
REMARK 465 GLY T 222
REMARK 465 GLY T 223
REMARK 465 PRO T 224
REMARK 465 GLY T 225
REMARK 465 HIS T 226
REMARK 465 LYS T 227
REMARK 465 ALA T 228
REMARK 465 ARG T 229
REMARK 465 VAL T 230
REMARK 465 LEU T 231
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT
REMARK 500
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.
REMARK 500
REMARK 500 ATM1 RES C SSEQI ATM2 RES C SSEQI DISTANCE
REMARK 500 CA GLU C 180 CA GLU R 180 1.22
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS
REMARK 500
REMARK 500 THE FOLLOWING ATOMS THAT ARE RELATED BY CRYSTALLOGRAPHIC
REMARK 500 SYMMETRY ARE IN CLOSE CONTACT. AN ATOM LOCATED WITHIN 0.15
REMARK 500 ANGSTROMS OF A SYMMETRY RELATED ATOM IS ASSUMED TO BE ON A
REMARK 500 SPECIAL POSITION AND IS, THEREFORE, LISTED IN REMARK 375
REMARK 500 INSTEAD OF REMARK 500. ATOMS WITH NON-BLANK ALTERNATE
REMARK 500 LOCATION INDICATORS ARE NOT INCLUDED IN THE CALCULATIONS.
REMARK 500
REMARK 500 DISTANCE CUTOFF:
REMARK 500 2.2 ANGSTROMS FOR CONTACTS NOT INVOLVING HYDROGEN ATOMS
REMARK 500 1.6 ANGSTROMS FOR CONTACTS INVOLVING HYDROGEN ATOMS
REMARK 500
REMARK 500 ATM1 RES C SSEQI ATM2 RES C SSEQI SSYMOP DISTANCE
REMARK 500 CA ALA E 184 CA ALA T 184 1556 2.06
REMARK 500
REMARK 500 REMARK: NULL
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: 3P05 RELATED DB: PDB
REMARK 900 X-RAY STRUCTURE OF PENTAMERIC HIV-1 CA
DBREF 3P0A A 1 231 UNP Q72497 Q72497_9HIV1 133 363
DBREF 3P0A B 1 231 UNP Q72497 Q72497_9HIV1 133 363
DBREF 3P0A C 1 231 UNP Q72497 Q72497_9HIV1 133 363
DBREF 3P0A D 1 231 UNP Q72497 Q72497_9HIV1 133 363
DBREF 3P0A E 1 231 UNP Q72497 Q72497_9HIV1 133 363
DBREF 3P0A F 1 231 UNP Q72497 Q72497_9HIV1 133 363
DBREF 3P0A G 1 231 UNP Q72497 Q72497_9HIV1 133 363
DBREF 3P0A H 1 231 UNP Q72497 Q72497_9HIV1 133 363
DBREF 3P0A I 1 231 UNP Q72497 Q72497_9HIV1 133 363
DBREF 3P0A J 1 231 UNP Q72497 Q72497_9HIV1 133 363
DBREF 3P0A K 1 231 UNP Q72497 Q72497_9HIV1 133 363
DBREF 3P0A L 1 231 UNP Q72497 Q72497_9HIV1 133 363
DBREF 3P0A M 1 231 UNP Q72497 Q72497_9HIV1 133 363
DBREF 3P0A N 1 231 UNP Q72497 Q72497_9HIV1 133 363
DBREF 3P0A O 1 231 UNP Q72497 Q72497_9HIV1 133 363
DBREF 3P0A P 1 231 UNP Q72497 Q72497_9HIV1 133 363
DBREF 3P0A Q 1 231 UNP Q72497 Q72497_9HIV1 133 363
DBREF 3P0A R 1 231 UNP Q72497 Q72497_9HIV1 133 363
DBREF 3P0A S 1 231 UNP Q72497 Q72497_9HIV1 133 363
DBREF 3P0A T 1 231 UNP Q72497 Q72497_9HIV1 133 363
SEQADV 3P0A CYS A 17 UNP Q72497 PRO 149 ENGINEERED MUTATION
SEQADV 3P0A LEU A 18 UNP Q72497 ARG 150 ENGINEERED MUTATION
SEQADV 3P0A CYS A 19 UNP Q72497 THR 151 ENGINEERED MUTATION
SEQADV 3P0A ALA A 184 UNP Q72497 TRP 316 ENGINEERED MUTATION
SEQADV 3P0A ALA A 185 UNP Q72497 MET 317 ENGINEERED MUTATION
SEQADV 3P0A CYS B 17 UNP Q72497 PRO 149 ENGINEERED MUTATION
SEQADV 3P0A LEU B 18 UNP Q72497 ARG 150 ENGINEERED MUTATION
SEQADV 3P0A CYS B 19 UNP Q72497 THR 151 ENGINEERED MUTATION
SEQADV 3P0A ALA B 184 UNP Q72497 TRP 316 ENGINEERED MUTATION
SEQADV 3P0A ALA B 185 UNP Q72497 MET 317 ENGINEERED MUTATION
SEQADV 3P0A CYS C 17 UNP Q72497 PRO 149 ENGINEERED MUTATION
SEQADV 3P0A LEU C 18 UNP Q72497 ARG 150 ENGINEERED MUTATION
SEQADV 3P0A CYS C 19 UNP Q72497 THR 151 ENGINEERED MUTATION
SEQADV 3P0A ALA C 184 UNP Q72497 TRP 316 ENGINEERED MUTATION
SEQADV 3P0A ALA C 185 UNP Q72497 MET 317 ENGINEERED MUTATION
SEQADV 3P0A CYS D 17 UNP Q72497 PRO 149 ENGINEERED MUTATION
SEQADV 3P0A LEU D 18 UNP Q72497 ARG 150 ENGINEERED MUTATION
SEQADV 3P0A CYS D 19 UNP Q72497 THR 151 ENGINEERED MUTATION
SEQADV 3P0A ALA D 184 UNP Q72497 TRP 316 ENGINEERED MUTATION
SEQADV 3P0A ALA D 185 UNP Q72497 MET 317 ENGINEERED MUTATION
SEQADV 3P0A CYS E 17 UNP Q72497 PRO 149 ENGINEERED MUTATION
SEQADV 3P0A LEU E 18 UNP Q72497 ARG 150 ENGINEERED MUTATION
SEQADV 3P0A CYS E 19 UNP Q72497 THR 151 ENGINEERED MUTATION
SEQADV 3P0A ALA E 184 UNP Q72497 TRP 316 ENGINEERED MUTATION
SEQADV 3P0A ALA E 185 UNP Q72497 MET 317 ENGINEERED MUTATION
SEQADV 3P0A CYS F 17 UNP Q72497 PRO 149 ENGINEERED MUTATION
SEQADV 3P0A LEU F 18 UNP Q72497 ARG 150 ENGINEERED MUTATION
SEQADV 3P0A CYS F 19 UNP Q72497 THR 151 ENGINEERED MUTATION
SEQADV 3P0A ALA F 184 UNP Q72497 TRP 316 ENGINEERED MUTATION
SEQADV 3P0A ALA F 185 UNP Q72497 MET 317 ENGINEERED MUTATION
SEQADV 3P0A CYS G 17 UNP Q72497 PRO 149 ENGINEERED MUTATION
SEQADV 3P0A LEU G 18 UNP Q72497 ARG 150 ENGINEERED MUTATION
SEQADV 3P0A CYS G 19 UNP Q72497 THR 151 ENGINEERED MUTATION
SEQADV 3P0A ALA G 184 UNP Q72497 TRP 316 ENGINEERED MUTATION
SEQADV 3P0A ALA G 185 UNP Q72497 MET 317 ENGINEERED MUTATION
SEQADV 3P0A CYS H 17 UNP Q72497 PRO 149 ENGINEERED MUTATION
SEQADV 3P0A LEU H 18 UNP Q72497 ARG 150 ENGINEERED MUTATION
SEQADV 3P0A CYS H 19 UNP Q72497 THR 151 ENGINEERED MUTATION
SEQADV 3P0A ALA H 184 UNP Q72497 TRP 316 ENGINEERED MUTATION
SEQADV 3P0A ALA H 185 UNP Q72497 MET 317 ENGINEERED MUTATION
SEQADV 3P0A CYS I 17 UNP Q72497 PRO 149 ENGINEERED MUTATION
SEQADV 3P0A LEU I 18 UNP Q72497 ARG 150 ENGINEERED MUTATION
SEQADV 3P0A CYS I 19 UNP Q72497 THR 151 ENGINEERED MUTATION
SEQADV 3P0A ALA I 184 UNP Q72497 TRP 316 ENGINEERED MUTATION
SEQADV 3P0A ALA I 185 UNP Q72497 MET 317 ENGINEERED MUTATION
SEQADV 3P0A CYS J 17 UNP Q72497 PRO 149 ENGINEERED MUTATION
SEQADV 3P0A LEU J 18 UNP Q72497 ARG 150 ENGINEERED MUTATION
SEQADV 3P0A CYS J 19 UNP Q72497 THR 151 ENGINEERED MUTATION
SEQADV 3P0A ALA J 184 UNP Q72497 TRP 316 ENGINEERED MUTATION
SEQADV 3P0A ALA J 185 UNP Q72497 MET 317 ENGINEERED MUTATION
SEQADV 3P0A CYS K 17 UNP Q72497 PRO 149 ENGINEERED MUTATION
SEQADV 3P0A LEU K 18 UNP Q72497 ARG 150 ENGINEERED MUTATION
SEQADV 3P0A CYS K 19 UNP Q72497 THR 151 ENGINEERED MUTATION
SEQADV 3P0A ALA K 184 UNP Q72497 TRP 316 ENGINEERED MUTATION
SEQADV 3P0A ALA K 185 UNP Q72497 MET 317 ENGINEERED MUTATION
SEQADV 3P0A CYS L 17 UNP Q72497 PRO 149 ENGINEERED MUTATION
SEQADV 3P0A LEU L 18 UNP Q72497 ARG 150 ENGINEERED MUTATION
SEQADV 3P0A CYS L 19 UNP Q72497 THR 151 ENGINEERED MUTATION
SEQADV 3P0A ALA L 184 UNP Q72497 TRP 316 ENGINEERED MUTATION
SEQADV 3P0A ALA L 185 UNP Q72497 MET 317 ENGINEERED MUTATION
SEQADV 3P0A CYS M 17 UNP Q72497 PRO 149 ENGINEERED MUTATION
SEQADV 3P0A LEU M 18 UNP Q72497 ARG 150 ENGINEERED MUTATION
SEQADV 3P0A CYS M 19 UNP Q72497 THR 151 ENGINEERED MUTATION
SEQADV 3P0A ALA M 184 UNP Q72497 TRP 316 ENGINEERED MUTATION
SEQADV 3P0A ALA M 185 UNP Q72497 MET 317 ENGINEERED MUTATION
SEQADV 3P0A CYS N 17 UNP Q72497 PRO 149 ENGINEERED MUTATION
SEQADV 3P0A LEU N 18 UNP Q72497 ARG 150 ENGINEERED MUTATION
SEQADV 3P0A CYS N 19 UNP Q72497 THR 151 ENGINEERED MUTATION
SEQADV 3P0A ALA N 184 UNP Q72497 TRP 316 ENGINEERED MUTATION
SEQADV 3P0A ALA N 185 UNP Q72497 MET 317 ENGINEERED MUTATION
SEQADV 3P0A CYS O 17 UNP Q72497 PRO 149 ENGINEERED MUTATION
SEQADV 3P0A LEU O 18 UNP Q72497 ARG 150 ENGINEERED MUTATION
SEQADV 3P0A CYS O 19 UNP Q72497 THR 151 ENGINEERED MUTATION
SEQADV 3P0A ALA O 184 UNP Q72497 TRP 316 ENGINEERED MUTATION
SEQADV 3P0A ALA O 185 UNP Q72497 MET 317 ENGINEERED MUTATION
SEQADV 3P0A CYS P 17 UNP Q72497 PRO 149 ENGINEERED MUTATION
SEQADV 3P0A LEU P 18 UNP Q72497 ARG 150 ENGINEERED MUTATION
SEQADV 3P0A CYS P 19 UNP Q72497 THR 151 ENGINEERED MUTATION
SEQADV 3P0A ALA P 184 UNP Q72497 TRP 316 ENGINEERED MUTATION
SEQADV 3P0A ALA P 185 UNP Q72497 MET 317 ENGINEERED MUTATION
SEQADV 3P0A CYS Q 17 UNP Q72497 PRO 149 ENGINEERED MUTATION
SEQADV 3P0A LEU Q 18 UNP Q72497 ARG 150 ENGINEERED MUTATION
SEQADV 3P0A CYS Q 19 UNP Q72497 THR 151 ENGINEERED MUTATION
SEQADV 3P0A ALA Q 184 UNP Q72497 TRP 316 ENGINEERED MUTATION
SEQADV 3P0A ALA Q 185 UNP Q72497 MET 317 ENGINEERED MUTATION
SEQADV 3P0A CYS R 17 UNP Q72497 PRO 149 ENGINEERED MUTATION
SEQADV 3P0A LEU R 18 UNP Q72497 ARG 150 ENGINEERED MUTATION
SEQADV 3P0A CYS R 19 UNP Q72497 THR 151 ENGINEERED MUTATION
SEQADV 3P0A ALA R 184 UNP Q72497 TRP 316 ENGINEERED MUTATION
SEQADV 3P0A ALA R 185 UNP Q72497 MET 317 ENGINEERED MUTATION
SEQADV 3P0A CYS S 17 UNP Q72497 PRO 149 ENGINEERED MUTATION
SEQADV 3P0A LEU S 18 UNP Q72497 ARG 150 ENGINEERED MUTATION
SEQADV 3P0A CYS S 19 UNP Q72497 THR 151 ENGINEERED MUTATION
SEQADV 3P0A ALA S 184 UNP Q72497 TRP 316 ENGINEERED MUTATION
SEQADV 3P0A ALA S 185 UNP Q72497 MET 317 ENGINEERED MUTATION
SEQADV 3P0A CYS T 17 UNP Q72497 PRO 149 ENGINEERED MUTATION
SEQADV 3P0A LEU T 18 UNP Q72497 ARG 150 ENGINEERED MUTATION
SEQADV 3P0A CYS T 19 UNP Q72497 THR 151 ENGINEERED MUTATION
SEQADV 3P0A ALA T 184 UNP Q72497 TRP 316 ENGINEERED MUTATION
SEQADV 3P0A ALA T 185 UNP Q72497 MET 317 ENGINEERED MUTATION
SEQRES 1 A 231 PRO ILE VAL GLN ASN LEU GLN GLY GLN MET VAL HIS GLN
SEQRES 2 A 231 ALA ILE SER CYS LEU CYS LEU ASN ALA TRP VAL LYS VAL
SEQRES 3 A 231 VAL GLU GLU LYS ALA PHE SER PRO GLU VAL ILE PRO MET
SEQRES 4 A 231 PHE SER ALA LEU SER GLU GLY ALA THR PRO GLN ASP LEU
SEQRES 5 A 231 ASN THR MET LEU ASN THR VAL GLY GLY HIS GLN ALA ALA
SEQRES 6 A 231 MET GLN MET LEU LYS GLU THR ILE ASN GLU GLU ALA ALA
SEQRES 7 A 231 GLU TRP ASP ARG LEU HIS PRO VAL HIS ALA GLY PRO ILE
SEQRES 8 A 231 ALA PRO GLY GLN MET ARG GLU PRO ARG GLY SER ASP ILE
SEQRES 9 A 231 ALA GLY THR THR SER THR LEU GLN GLU GLN ILE GLY TRP
SEQRES 10 A 231 MET THR HIS ASN PRO PRO ILE PRO VAL GLY GLU ILE TYR
SEQRES 11 A 231 LYS ARG TRP ILE ILE LEU GLY LEU ASN LYS ILE VAL ARG
SEQRES 12 A 231 MET TYR SER PRO THR SER ILE LEU ASP ILE ARG GLN GLY
SEQRES 13 A 231 PRO LYS GLU PRO PHE ARG ASP TYR VAL ASP ARG PHE TYR
SEQRES 14 A 231 LYS THR LEU ARG ALA GLU GLN ALA SER GLN GLU VAL LYS
SEQRES 15 A 231 ASN ALA ALA THR GLU THR LEU LEU VAL GLN ASN ALA ASN
SEQRES 16 A 231 PRO ASP CYS LYS THR ILE LEU LYS ALA LEU GLY PRO GLY
SEQRES 17 A 231 ALA THR LEU GLU GLU MET MET THR ALA CYS GLN GLY VAL
SEQRES 18 A 231 GLY GLY PRO GLY HIS LYS ALA ARG VAL LEU
SEQRES 1 B 231 PRO ILE VAL GLN ASN LEU GLN GLY GLN MET VAL HIS GLN
SEQRES 2 B 231 ALA ILE SER CYS LEU CYS LEU ASN ALA TRP VAL LYS VAL
SEQRES 3 B 231 VAL GLU GLU LYS ALA PHE SER PRO GLU VAL ILE PRO MET
SEQRES 4 B 231 PHE SER ALA LEU SER GLU GLY ALA THR PRO GLN ASP LEU
SEQRES 5 B 231 ASN THR MET LEU ASN THR VAL GLY GLY HIS GLN ALA ALA
SEQRES 6 B 231 MET GLN MET LEU LYS GLU THR ILE ASN GLU GLU ALA ALA
SEQRES 7 B 231 GLU TRP ASP ARG LEU HIS PRO VAL HIS ALA GLY PRO ILE
SEQRES 8 B 231 ALA PRO GLY GLN MET ARG GLU PRO ARG GLY SER ASP ILE
SEQRES 9 B 231 ALA GLY THR THR SER THR LEU GLN GLU GLN ILE GLY TRP
SEQRES 10 B 231 MET THR HIS ASN PRO PRO ILE PRO VAL GLY GLU ILE TYR
SEQRES 11 B 231 LYS ARG TRP ILE ILE LEU GLY LEU ASN LYS ILE VAL ARG
SEQRES 12 B 231 MET TYR SER PRO THR SER ILE LEU ASP ILE ARG GLN GLY
SEQRES 13 B 231 PRO LYS GLU PRO PHE ARG ASP TYR VAL ASP ARG PHE TYR
SEQRES 14 B 231 LYS THR LEU ARG ALA GLU GLN ALA SER GLN GLU VAL LYS
SEQRES 15 B 231 ASN ALA ALA THR GLU THR LEU LEU VAL GLN ASN ALA ASN
SEQRES 16 B 231 PRO ASP CYS LYS THR ILE LEU LYS ALA LEU GLY PRO GLY
SEQRES 17 B 231 ALA THR LEU GLU GLU MET MET THR ALA CYS GLN GLY VAL
SEQRES 18 B 231 GLY GLY PRO GLY HIS LYS ALA ARG VAL LEU
SEQRES 1 C 231 PRO ILE VAL GLN ASN LEU GLN GLY GLN MET VAL HIS GLN
SEQRES 2 C 231 ALA ILE SER CYS LEU CYS LEU ASN ALA TRP VAL LYS VAL
SEQRES 3 C 231 VAL GLU GLU LYS ALA PHE SER PRO GLU VAL ILE PRO MET
SEQRES 4 C 231 PHE SER ALA LEU SER GLU GLY ALA THR PRO GLN ASP LEU
SEQRES 5 C 231 ASN THR MET LEU ASN THR VAL GLY GLY HIS GLN ALA ALA
SEQRES 6 C 231 MET GLN MET LEU LYS GLU THR ILE ASN GLU GLU ALA ALA
SEQRES 7 C 231 GLU TRP ASP ARG LEU HIS PRO VAL HIS ALA GLY PRO ILE
SEQRES 8 C 231 ALA PRO GLY GLN MET ARG GLU PRO ARG GLY SER ASP ILE
SEQRES 9 C 231 ALA GLY THR THR SER THR LEU GLN GLU GLN ILE GLY TRP
SEQRES 10 C 231 MET THR HIS ASN PRO PRO ILE PRO VAL GLY GLU ILE TYR
SEQRES 11 C 231 LYS ARG TRP ILE ILE LEU GLY LEU ASN LYS ILE VAL ARG
SEQRES 12 C 231 MET TYR SER PRO THR SER ILE LEU ASP ILE ARG GLN GLY
SEQRES 13 C 231 PRO LYS GLU PRO PHE ARG ASP TYR VAL ASP ARG PHE TYR
SEQRES 14 C 231 LYS THR LEU ARG ALA GLU GLN ALA SER GLN GLU VAL LYS
SEQRES 15 C 231 ASN ALA ALA THR GLU THR LEU LEU VAL GLN ASN ALA ASN
SEQRES 16 C 231 PRO ASP CYS LYS THR ILE LEU LYS ALA LEU GLY PRO GLY
SEQRES 17 C 231 ALA THR LEU GLU GLU MET MET THR ALA CYS GLN GLY VAL
SEQRES 18 C 231 GLY GLY PRO GLY HIS LYS ALA ARG VAL LEU
SEQRES 1 D 231 PRO ILE VAL GLN ASN LEU GLN GLY GLN MET VAL HIS GLN
SEQRES 2 D 231 ALA ILE SER CYS LEU CYS LEU ASN ALA TRP VAL LYS VAL
SEQRES 3 D 231 VAL GLU GLU LYS ALA PHE SER PRO GLU VAL ILE PRO MET
SEQRES 4 D 231 PHE SER ALA LEU SER GLU GLY ALA THR PRO GLN ASP LEU
SEQRES 5 D 231 ASN THR MET LEU ASN THR VAL GLY GLY HIS GLN ALA ALA
SEQRES 6 D 231 MET GLN MET LEU LYS GLU THR ILE ASN GLU GLU ALA ALA
SEQRES 7 D 231 GLU TRP ASP ARG LEU HIS PRO VAL HIS ALA GLY PRO ILE
SEQRES 8 D 231 ALA PRO GLY GLN MET ARG GLU PRO ARG GLY SER ASP ILE
SEQRES 9 D 231 ALA GLY THR THR SER THR LEU GLN GLU GLN ILE GLY TRP
SEQRES 10 D 231 MET THR HIS ASN PRO PRO ILE PRO VAL GLY GLU ILE TYR
SEQRES 11 D 231 LYS ARG TRP ILE ILE LEU GLY LEU ASN LYS ILE VAL ARG
SEQRES 12 D 231 MET TYR SER PRO THR SER ILE LEU ASP ILE ARG GLN GLY
SEQRES 13 D 231 PRO LYS GLU PRO PHE ARG ASP TYR VAL ASP ARG PHE TYR
SEQRES 14 D 231 LYS THR LEU ARG ALA GLU GLN ALA SER GLN GLU VAL LYS
SEQRES 15 D 231 ASN ALA ALA THR GLU THR LEU LEU VAL GLN ASN ALA ASN
SEQRES 16 D 231 PRO ASP CYS LYS THR ILE LEU LYS ALA LEU GLY PRO GLY
SEQRES 17 D 231 ALA THR LEU GLU GLU MET MET THR ALA CYS GLN GLY VAL
SEQRES 18 D 231 GLY GLY PRO GLY HIS LYS ALA ARG VAL LEU
SEQRES 1 E 231 PRO ILE VAL GLN ASN LEU GLN GLY GLN MET VAL HIS GLN
SEQRES 2 E 231 ALA ILE SER CYS LEU CYS LEU ASN ALA TRP VAL LYS VAL
SEQRES 3 E 231 VAL GLU GLU LYS ALA PHE SER PRO GLU VAL ILE PRO MET
SEQRES 4 E 231 PHE SER ALA LEU SER GLU GLY ALA THR PRO GLN ASP LEU
SEQRES 5 E 231 ASN THR MET LEU ASN THR VAL GLY GLY HIS GLN ALA ALA
SEQRES 6 E 231 MET GLN MET LEU LYS GLU THR ILE ASN GLU GLU ALA ALA
SEQRES 7 E 231 GLU TRP ASP ARG LEU HIS PRO VAL HIS ALA GLY PRO ILE
SEQRES 8 E 231 ALA PRO GLY GLN MET ARG GLU PRO ARG GLY SER ASP ILE
SEQRES 9 E 231 ALA GLY THR THR SER THR LEU GLN GLU GLN ILE GLY TRP
SEQRES 10 E 231 MET THR HIS ASN PRO PRO ILE PRO VAL GLY GLU ILE TYR
SEQRES 11 E 231 LYS ARG TRP ILE ILE LEU GLY LEU ASN LYS ILE VAL ARG
SEQRES 12 E 231 MET TYR SER PRO THR SER ILE LEU ASP ILE ARG GLN GLY
SEQRES 13 E 231 PRO LYS GLU PRO PHE ARG ASP TYR VAL ASP ARG PHE TYR
SEQRES 14 E 231 LYS THR LEU ARG ALA GLU GLN ALA SER GLN GLU VAL LYS
SEQRES 15 E 231 ASN ALA ALA THR GLU THR LEU LEU VAL GLN ASN ALA ASN
SEQRES 16 E 231 PRO ASP CYS LYS THR ILE LEU LYS ALA LEU GLY PRO GLY
SEQRES 17 E 231 ALA THR LEU GLU GLU MET MET THR ALA CYS GLN GLY VAL
SEQRES 18 E 231 GLY GLY PRO GLY HIS LYS ALA ARG VAL LEU
SEQRES 1 F 231 PRO ILE VAL GLN ASN LEU GLN GLY GLN MET VAL HIS GLN
SEQRES 2 F 231 ALA ILE SER CYS LEU CYS LEU ASN ALA TRP VAL LYS VAL
SEQRES 3 F 231 VAL GLU GLU LYS ALA PHE SER PRO GLU VAL ILE PRO MET
SEQRES 4 F 231 PHE SER ALA LEU SER GLU GLY ALA THR PRO GLN ASP LEU
SEQRES 5 F 231 ASN THR MET LEU ASN THR VAL GLY GLY HIS GLN ALA ALA
SEQRES 6 F 231 MET GLN MET LEU LYS GLU THR ILE ASN GLU GLU ALA ALA
SEQRES 7 F 231 GLU TRP ASP ARG LEU HIS PRO VAL HIS ALA GLY PRO ILE
SEQRES 8 F 231 ALA PRO GLY GLN MET ARG GLU PRO ARG GLY SER ASP ILE
SEQRES 9 F 231 ALA GLY THR THR SER THR LEU GLN GLU GLN ILE GLY TRP
SEQRES 10 F 231 MET THR HIS ASN PRO PRO ILE PRO VAL GLY GLU ILE TYR
SEQRES 11 F 231 LYS ARG TRP ILE ILE LEU GLY LEU ASN LYS ILE VAL ARG
SEQRES 12 F 231 MET TYR SER PRO THR SER ILE LEU ASP ILE ARG GLN GLY
SEQRES 13 F 231 PRO LYS GLU PRO PHE ARG ASP TYR VAL ASP ARG PHE TYR
SEQRES 14 F 231 LYS THR LEU ARG ALA GLU GLN ALA SER GLN GLU VAL LYS
SEQRES 15 F 231 ASN ALA ALA THR GLU THR LEU LEU VAL GLN ASN ALA ASN
SEQRES 16 F 231 PRO ASP CYS LYS THR ILE LEU LYS ALA LEU GLY PRO GLY
SEQRES 17 F 231 ALA THR LEU GLU GLU MET MET THR ALA CYS GLN GLY VAL
SEQRES 18 F 231 GLY GLY PRO GLY HIS LYS ALA ARG VAL LEU
SEQRES 1 G 231 PRO ILE VAL GLN ASN LEU GLN GLY GLN MET VAL HIS GLN
SEQRES 2 G 231 ALA ILE SER CYS LEU CYS LEU ASN ALA TRP VAL LYS VAL
SEQRES 3 G 231 VAL GLU GLU LYS ALA PHE SER PRO GLU VAL ILE PRO MET
SEQRES 4 G 231 PHE SER ALA LEU SER GLU GLY ALA THR PRO GLN ASP LEU
SEQRES 5 G 231 ASN THR MET LEU ASN THR VAL GLY GLY HIS GLN ALA ALA
SEQRES 6 G 231 MET GLN MET LEU LYS GLU THR ILE ASN GLU GLU ALA ALA
SEQRES 7 G 231 GLU TRP ASP ARG LEU HIS PRO VAL HIS ALA GLY PRO ILE
SEQRES 8 G 231 ALA PRO GLY GLN MET ARG GLU PRO ARG GLY SER ASP ILE
SEQRES 9 G 231 ALA GLY THR THR SER THR LEU GLN GLU GLN ILE GLY TRP
SEQRES 10 G 231 MET THR HIS ASN PRO PRO ILE PRO VAL GLY GLU ILE TYR
SEQRES 11 G 231 LYS ARG TRP ILE ILE LEU GLY LEU ASN LYS ILE VAL ARG
SEQRES 12 G 231 MET TYR SER PRO THR SER ILE LEU ASP ILE ARG GLN GLY
SEQRES 13 G 231 PRO LYS GLU PRO PHE ARG ASP TYR VAL ASP ARG PHE TYR
SEQRES 14 G 231 LYS THR LEU ARG ALA GLU GLN ALA SER GLN GLU VAL LYS
SEQRES 15 G 231 ASN ALA ALA THR GLU THR LEU LEU VAL GLN ASN ALA ASN
SEQRES 16 G 231 PRO ASP CYS LYS THR ILE LEU LYS ALA LEU GLY PRO GLY
SEQRES 17 G 231 ALA THR LEU GLU GLU MET MET THR ALA CYS GLN GLY VAL
SEQRES 18 G 231 GLY GLY PRO GLY HIS LYS ALA ARG VAL LEU
SEQRES 1 H 231 PRO ILE VAL GLN ASN LEU GLN GLY GLN MET VAL HIS GLN
SEQRES 2 H 231 ALA ILE SER CYS LEU CYS LEU ASN ALA TRP VAL LYS VAL
SEQRES 3 H 231 VAL GLU GLU LYS ALA PHE SER PRO GLU VAL ILE PRO MET
SEQRES 4 H 231 PHE SER ALA LEU SER GLU GLY ALA THR PRO GLN ASP LEU
SEQRES 5 H 231 ASN THR MET LEU ASN THR VAL GLY GLY HIS GLN ALA ALA
SEQRES 6 H 231 MET GLN MET LEU LYS GLU THR ILE ASN GLU GLU ALA ALA
SEQRES 7 H 231 GLU TRP ASP ARG LEU HIS PRO VAL HIS ALA GLY PRO ILE
SEQRES 8 H 231 ALA PRO GLY GLN MET ARG GLU PRO ARG GLY SER ASP ILE
SEQRES 9 H 231 ALA GLY THR THR SER THR LEU GLN GLU GLN ILE GLY TRP
SEQRES 10 H 231 MET THR HIS ASN PRO PRO ILE PRO VAL GLY GLU ILE TYR
SEQRES 11 H 231 LYS ARG TRP ILE ILE LEU GLY LEU ASN LYS ILE VAL ARG
SEQRES 12 H 231 MET TYR SER PRO THR SER ILE LEU ASP ILE ARG GLN GLY
SEQRES 13 H 231 PRO LYS GLU PRO PHE ARG ASP TYR VAL ASP ARG PHE TYR
SEQRES 14 H 231 LYS THR LEU ARG ALA GLU GLN ALA SER GLN GLU VAL LYS
SEQRES 15 H 231 ASN ALA ALA THR GLU THR LEU LEU VAL GLN ASN ALA ASN
SEQRES 16 H 231 PRO ASP CYS LYS THR ILE LEU LYS ALA LEU GLY PRO GLY
SEQRES 17 H 231 ALA THR LEU GLU GLU MET MET THR ALA CYS GLN GLY VAL
SEQRES 18 H 231 GLY GLY PRO GLY HIS LYS ALA ARG VAL LEU
SEQRES 1 I 231 PRO ILE VAL GLN ASN LEU GLN GLY GLN MET VAL HIS GLN
SEQRES 2 I 231 ALA ILE SER CYS LEU CYS LEU ASN ALA TRP VAL LYS VAL
SEQRES 3 I 231 VAL GLU GLU LYS ALA PHE SER PRO GLU VAL ILE PRO MET
SEQRES 4 I 231 PHE SER ALA LEU SER GLU GLY ALA THR PRO GLN ASP LEU
SEQRES 5 I 231 ASN THR MET LEU ASN THR VAL GLY GLY HIS GLN ALA ALA
SEQRES 6 I 231 MET GLN MET LEU LYS GLU THR ILE ASN GLU GLU ALA ALA
SEQRES 7 I 231 GLU TRP ASP ARG LEU HIS PRO VAL HIS ALA GLY PRO ILE
SEQRES 8 I 231 ALA PRO GLY GLN MET ARG GLU PRO ARG GLY SER ASP ILE
SEQRES 9 I 231 ALA GLY THR THR SER THR LEU GLN GLU GLN ILE GLY TRP
SEQRES 10 I 231 MET THR HIS ASN PRO PRO ILE PRO VAL GLY GLU ILE TYR
SEQRES 11 I 231 LYS ARG TRP ILE ILE LEU GLY LEU ASN LYS ILE VAL ARG
SEQRES 12 I 231 MET TYR SER PRO THR SER ILE LEU ASP ILE ARG GLN GLY
SEQRES 13 I 231 PRO LYS GLU PRO PHE ARG ASP TYR VAL ASP ARG PHE TYR
SEQRES 14 I 231 LYS THR LEU ARG ALA GLU GLN ALA SER GLN GLU VAL LYS
SEQRES 15 I 231 ASN ALA ALA THR GLU THR LEU LEU VAL GLN ASN ALA ASN
SEQRES 16 I 231 PRO ASP CYS LYS THR ILE LEU LYS ALA LEU GLY PRO GLY
SEQRES 17 I 231 ALA THR LEU GLU GLU MET MET THR ALA CYS GLN GLY VAL
SEQRES 18 I 231 GLY GLY PRO GLY HIS LYS ALA ARG VAL LEU
SEQRES 1 J 231 PRO ILE VAL GLN ASN LEU GLN GLY GLN MET VAL HIS GLN
SEQRES 2 J 231 ALA ILE SER CYS LEU CYS LEU ASN ALA TRP VAL LYS VAL
SEQRES 3 J 231 VAL GLU GLU LYS ALA PHE SER PRO GLU VAL ILE PRO MET
SEQRES 4 J 231 PHE SER ALA LEU SER GLU GLY ALA THR PRO GLN ASP LEU
SEQRES 5 J 231 ASN THR MET LEU ASN THR VAL GLY GLY HIS GLN ALA ALA
SEQRES 6 J 231 MET GLN MET LEU LYS GLU THR ILE ASN GLU GLU ALA ALA
SEQRES 7 J 231 GLU TRP ASP ARG LEU HIS PRO VAL HIS ALA GLY PRO ILE
SEQRES 8 J 231 ALA PRO GLY GLN MET ARG GLU PRO ARG GLY SER ASP ILE
SEQRES 9 J 231 ALA GLY THR THR SER THR LEU GLN GLU GLN ILE GLY TRP
SEQRES 10 J 231 MET THR HIS ASN PRO PRO ILE PRO VAL GLY GLU ILE TYR
SEQRES 11 J 231 LYS ARG TRP ILE ILE LEU GLY LEU ASN LYS ILE VAL ARG
SEQRES 12 J 231 MET TYR SER PRO THR SER ILE LEU ASP ILE ARG GLN GLY
SEQRES 13 J 231 PRO LYS GLU PRO PHE ARG ASP TYR VAL ASP ARG PHE TYR
SEQRES 14 J 231 LYS THR LEU ARG ALA GLU GLN ALA SER GLN GLU VAL LYS
SEQRES 15 J 231 ASN ALA ALA THR GLU THR LEU LEU VAL GLN ASN ALA ASN
SEQRES 16 J 231 PRO ASP CYS LYS THR ILE LEU LYS ALA LEU GLY PRO GLY
SEQRES 17 J 231 ALA THR LEU GLU GLU MET MET THR ALA CYS GLN GLY VAL
SEQRES 18 J 231 GLY GLY PRO GLY HIS LYS ALA ARG VAL LEU
SEQRES 1 K 231 PRO ILE VAL GLN ASN LEU GLN GLY GLN MET VAL HIS GLN
SEQRES 2 K 231 ALA ILE SER CYS LEU CYS LEU ASN ALA TRP VAL LYS VAL
SEQRES 3 K 231 VAL GLU GLU LYS ALA PHE SER PRO GLU VAL ILE PRO MET
SEQRES 4 K 231 PHE SER ALA LEU SER GLU GLY ALA THR PRO GLN ASP LEU
SEQRES 5 K 231 ASN THR MET LEU ASN THR VAL GLY GLY HIS GLN ALA ALA
SEQRES 6 K 231 MET GLN MET LEU LYS GLU THR ILE ASN GLU GLU ALA ALA
SEQRES 7 K 231 GLU TRP ASP ARG LEU HIS PRO VAL HIS ALA GLY PRO ILE
SEQRES 8 K 231 ALA PRO GLY GLN MET ARG GLU PRO ARG GLY SER ASP ILE
SEQRES 9 K 231 ALA GLY THR THR SER THR LEU GLN GLU GLN ILE GLY TRP
SEQRES 10 K 231 MET THR HIS ASN PRO PRO ILE PRO VAL GLY GLU ILE TYR
SEQRES 11 K 231 LYS ARG TRP ILE ILE LEU GLY LEU ASN LYS ILE VAL ARG
SEQRES 12 K 231 MET TYR SER PRO THR SER ILE LEU ASP ILE ARG GLN GLY
SEQRES 13 K 231 PRO LYS GLU PRO PHE ARG ASP TYR VAL ASP ARG PHE TYR
SEQRES 14 K 231 LYS THR LEU ARG ALA GLU GLN ALA SER GLN GLU VAL LYS
SEQRES 15 K 231 ASN ALA ALA THR GLU THR LEU LEU VAL GLN ASN ALA ASN
SEQRES 16 K 231 PRO ASP CYS LYS THR ILE LEU LYS ALA LEU GLY PRO GLY
SEQRES 17 K 231 ALA THR LEU GLU GLU MET MET THR ALA CYS GLN GLY VAL
SEQRES 18 K 231 GLY GLY PRO GLY HIS LYS ALA ARG VAL LEU
SEQRES 1 L 231 PRO ILE VAL GLN ASN LEU GLN GLY GLN MET VAL HIS GLN
SEQRES 2 L 231 ALA ILE SER CYS LEU CYS LEU ASN ALA TRP VAL LYS VAL
SEQRES 3 L 231 VAL GLU GLU LYS ALA PHE SER PRO GLU VAL ILE PRO MET
SEQRES 4 L 231 PHE SER ALA LEU SER GLU GLY ALA THR PRO GLN ASP LEU
SEQRES 5 L 231 ASN THR MET LEU ASN THR VAL GLY GLY HIS GLN ALA ALA
SEQRES 6 L 231 MET GLN MET LEU LYS GLU THR ILE ASN GLU GLU ALA ALA
SEQRES 7 L 231 GLU TRP ASP ARG LEU HIS PRO VAL HIS ALA GLY PRO ILE
SEQRES 8 L 231 ALA PRO GLY GLN MET ARG GLU PRO ARG GLY SER ASP ILE
SEQRES 9 L 231 ALA GLY THR THR SER THR LEU GLN GLU GLN ILE GLY TRP
SEQRES 10 L 231 MET THR HIS ASN PRO PRO ILE PRO VAL GLY GLU ILE TYR
SEQRES 11 L 231 LYS ARG TRP ILE ILE LEU GLY LEU ASN LYS ILE VAL ARG
SEQRES 12 L 231 MET TYR SER PRO THR SER ILE LEU ASP ILE ARG GLN GLY
SEQRES 13 L 231 PRO LYS GLU PRO PHE ARG ASP TYR VAL ASP ARG PHE TYR
SEQRES 14 L 231 LYS THR LEU ARG ALA GLU GLN ALA SER GLN GLU VAL LYS
SEQRES 15 L 231 ASN ALA ALA THR GLU THR LEU LEU VAL GLN ASN ALA ASN
SEQRES 16 L 231 PRO ASP CYS LYS THR ILE LEU LYS ALA LEU GLY PRO GLY
SEQRES 17 L 231 ALA THR LEU GLU GLU MET MET THR ALA CYS GLN GLY VAL
SEQRES 18 L 231 GLY GLY PRO GLY HIS LYS ALA ARG VAL LEU
SEQRES 1 M 231 PRO ILE VAL GLN ASN LEU GLN GLY GLN MET VAL HIS GLN
SEQRES 2 M 231 ALA ILE SER CYS LEU CYS LEU ASN ALA TRP VAL LYS VAL
SEQRES 3 M 231 VAL GLU GLU LYS ALA PHE SER PRO GLU VAL ILE PRO MET
SEQRES 4 M 231 PHE SER ALA LEU SER GLU GLY ALA THR PRO GLN ASP LEU
SEQRES 5 M 231 ASN THR MET LEU ASN THR VAL GLY GLY HIS GLN ALA ALA
SEQRES 6 M 231 MET GLN MET LEU LYS GLU THR ILE ASN GLU GLU ALA ALA
SEQRES 7 M 231 GLU TRP ASP ARG LEU HIS PRO VAL HIS ALA GLY PRO ILE
SEQRES 8 M 231 ALA PRO GLY GLN MET ARG GLU PRO ARG GLY SER ASP ILE
SEQRES 9 M 231 ALA GLY THR THR SER THR LEU GLN GLU GLN ILE GLY TRP
SEQRES 10 M 231 MET THR HIS ASN PRO PRO ILE PRO VAL GLY GLU ILE TYR
SEQRES 11 M 231 LYS ARG TRP ILE ILE LEU GLY LEU ASN LYS ILE VAL ARG
SEQRES 12 M 231 MET TYR SER PRO THR SER ILE LEU ASP ILE ARG GLN GLY
SEQRES 13 M 231 PRO LYS GLU PRO PHE ARG ASP TYR VAL ASP ARG PHE TYR
SEQRES 14 M 231 LYS THR LEU ARG ALA GLU GLN ALA SER GLN GLU VAL LYS
SEQRES 15 M 231 ASN ALA ALA THR GLU THR LEU LEU VAL GLN ASN ALA ASN
SEQRES 16 M 231 PRO ASP CYS LYS THR ILE LEU LYS ALA LEU GLY PRO GLY
SEQRES 17 M 231 ALA THR LEU GLU GLU MET MET THR ALA CYS GLN GLY VAL
SEQRES 18 M 231 GLY GLY PRO GLY HIS LYS ALA ARG VAL LEU
SEQRES 1 N 231 PRO ILE VAL GLN ASN LEU GLN GLY GLN MET VAL HIS GLN
SEQRES 2 N 231 ALA ILE SER CYS LEU CYS LEU ASN ALA TRP VAL LYS VAL
SEQRES 3 N 231 VAL GLU GLU LYS ALA PHE SER PRO GLU VAL ILE PRO MET
SEQRES 4 N 231 PHE SER ALA LEU SER GLU GLY ALA THR PRO GLN ASP LEU
SEQRES 5 N 231 ASN THR MET LEU ASN THR VAL GLY GLY HIS GLN ALA ALA
SEQRES 6 N 231 MET GLN MET LEU LYS GLU THR ILE ASN GLU GLU ALA ALA
SEQRES 7 N 231 GLU TRP ASP ARG LEU HIS PRO VAL HIS ALA GLY PRO ILE
SEQRES 8 N 231 ALA PRO GLY GLN MET ARG GLU PRO ARG GLY SER ASP ILE
SEQRES 9 N 231 ALA GLY THR THR SER THR LEU GLN GLU GLN ILE GLY TRP
SEQRES 10 N 231 MET THR HIS ASN PRO PRO ILE PRO VAL GLY GLU ILE TYR
SEQRES 11 N 231 LYS ARG TRP ILE ILE LEU GLY LEU ASN LYS ILE VAL ARG
SEQRES 12 N 231 MET TYR SER PRO THR SER ILE LEU ASP ILE ARG GLN GLY
SEQRES 13 N 231 PRO LYS GLU PRO PHE ARG ASP TYR VAL ASP ARG PHE TYR
SEQRES 14 N 231 LYS THR LEU ARG ALA GLU GLN ALA SER GLN GLU VAL LYS
SEQRES 15 N 231 ASN ALA ALA THR GLU THR LEU LEU VAL GLN ASN ALA ASN
SEQRES 16 N 231 PRO ASP CYS LYS THR ILE LEU LYS ALA LEU GLY PRO GLY
SEQRES 17 N 231 ALA THR LEU GLU GLU MET MET THR ALA CYS GLN GLY VAL
SEQRES 18 N 231 GLY GLY PRO GLY HIS LYS ALA ARG VAL LEU
SEQRES 1 O 231 PRO ILE VAL GLN ASN LEU GLN GLY GLN MET VAL HIS GLN
SEQRES 2 O 231 ALA ILE SER CYS LEU CYS LEU ASN ALA TRP VAL LYS VAL
SEQRES 3 O 231 VAL GLU GLU LYS ALA PHE SER PRO GLU VAL ILE PRO MET
SEQRES 4 O 231 PHE SER ALA LEU SER GLU GLY ALA THR PRO GLN ASP LEU
SEQRES 5 O 231 ASN THR MET LEU ASN THR VAL GLY GLY HIS GLN ALA ALA
SEQRES 6 O 231 MET GLN MET LEU LYS GLU THR ILE ASN GLU GLU ALA ALA
SEQRES 7 O 231 GLU TRP ASP ARG LEU HIS PRO VAL HIS ALA GLY PRO ILE
SEQRES 8 O 231 ALA PRO GLY GLN MET ARG GLU PRO ARG GLY SER ASP ILE
SEQRES 9 O 231 ALA GLY THR THR SER THR LEU GLN GLU GLN ILE GLY TRP
SEQRES 10 O 231 MET THR HIS ASN PRO PRO ILE PRO VAL GLY GLU ILE TYR
SEQRES 11 O 231 LYS ARG TRP ILE ILE LEU GLY LEU ASN LYS ILE VAL ARG
SEQRES 12 O 231 MET TYR SER PRO THR SER ILE LEU ASP ILE ARG GLN GLY
SEQRES 13 O 231 PRO LYS GLU PRO PHE ARG ASP TYR VAL ASP ARG PHE TYR
SEQRES 14 O 231 LYS THR LEU ARG ALA GLU GLN ALA SER GLN GLU VAL LYS
SEQRES 15 O 231 ASN ALA ALA THR GLU THR LEU LEU VAL GLN ASN ALA ASN
SEQRES 16 O 231 PRO ASP CYS LYS THR ILE LEU LYS ALA LEU GLY PRO GLY
SEQRES 17 O 231 ALA THR LEU GLU GLU MET MET THR ALA CYS GLN GLY VAL
SEQRES 18 O 231 GLY GLY PRO GLY HIS LYS ALA ARG VAL LEU
SEQRES 1 P 231 PRO ILE VAL GLN ASN LEU GLN GLY GLN MET VAL HIS GLN
SEQRES 2 P 231 ALA ILE SER CYS LEU CYS LEU ASN ALA TRP VAL LYS VAL
SEQRES 3 P 231 VAL GLU GLU LYS ALA PHE SER PRO GLU VAL ILE PRO MET
SEQRES 4 P 231 PHE SER ALA LEU SER GLU GLY ALA THR PRO GLN ASP LEU
SEQRES 5 P 231 ASN THR MET LEU ASN THR VAL GLY GLY HIS GLN ALA ALA
SEQRES 6 P 231 MET GLN MET LEU LYS GLU THR ILE ASN GLU GLU ALA ALA
SEQRES 7 P 231 GLU TRP ASP ARG LEU HIS PRO VAL HIS ALA GLY PRO ILE
SEQRES 8 P 231 ALA PRO GLY GLN MET ARG GLU PRO ARG GLY SER ASP ILE
SEQRES 9 P 231 ALA GLY THR THR SER THR LEU GLN GLU GLN ILE GLY TRP
SEQRES 10 P 231 MET THR HIS ASN PRO PRO ILE PRO VAL GLY GLU ILE TYR
SEQRES 11 P 231 LYS ARG TRP ILE ILE LEU GLY LEU ASN LYS ILE VAL ARG
SEQRES 12 P 231 MET TYR SER PRO THR SER ILE LEU ASP ILE ARG GLN GLY
SEQRES 13 P 231 PRO LYS GLU PRO PHE ARG ASP TYR VAL ASP ARG PHE TYR
SEQRES 14 P 231 LYS THR LEU ARG ALA GLU GLN ALA SER GLN GLU VAL LYS
SEQRES 15 P 231 ASN ALA ALA THR GLU THR LEU LEU VAL GLN ASN ALA ASN
SEQRES 16 P 231 PRO ASP CYS LYS THR ILE LEU LYS ALA LEU GLY PRO GLY
SEQRES 17 P 231 ALA THR LEU GLU GLU MET MET THR ALA CYS GLN GLY VAL
SEQRES 18 P 231 GLY GLY PRO GLY HIS LYS ALA ARG VAL LEU
SEQRES 1 Q 231 PRO ILE VAL GLN ASN LEU GLN GLY GLN MET VAL HIS GLN
SEQRES 2 Q 231 ALA ILE SER CYS LEU CYS LEU ASN ALA TRP VAL LYS VAL
SEQRES 3 Q 231 VAL GLU GLU LYS ALA PHE SER PRO GLU VAL ILE PRO MET
SEQRES 4 Q 231 PHE SER ALA LEU SER GLU GLY ALA THR PRO GLN ASP LEU
SEQRES 5 Q 231 ASN THR MET LEU ASN THR VAL GLY GLY HIS GLN ALA ALA
SEQRES 6 Q 231 MET GLN MET LEU LYS GLU THR ILE ASN GLU GLU ALA ALA
SEQRES 7 Q 231 GLU TRP ASP ARG LEU HIS PRO VAL HIS ALA GLY PRO ILE
SEQRES 8 Q 231 ALA PRO GLY GLN MET ARG GLU PRO ARG GLY SER ASP ILE
SEQRES 9 Q 231 ALA GLY THR THR SER THR LEU GLN GLU GLN ILE GLY TRP
SEQRES 10 Q 231 MET THR HIS ASN PRO PRO ILE PRO VAL GLY GLU ILE TYR
SEQRES 11 Q 231 LYS ARG TRP ILE ILE LEU GLY LEU ASN LYS ILE VAL ARG
SEQRES 12 Q 231 MET TYR SER PRO THR SER ILE LEU ASP ILE ARG GLN GLY
SEQRES 13 Q 231 PRO LYS GLU PRO PHE ARG ASP TYR VAL ASP ARG PHE TYR
SEQRES 14 Q 231 LYS THR LEU ARG ALA GLU GLN ALA SER GLN GLU VAL LYS
SEQRES 15 Q 231 ASN ALA ALA THR GLU THR LEU LEU VAL GLN ASN ALA ASN
SEQRES 16 Q 231 PRO ASP CYS LYS THR ILE LEU LYS ALA LEU GLY PRO GLY
SEQRES 17 Q 231 ALA THR LEU GLU GLU MET MET THR ALA CYS GLN GLY VAL
SEQRES 18 Q 231 GLY GLY PRO GLY HIS LYS ALA ARG VAL LEU
SEQRES 1 R 231 PRO ILE VAL GLN ASN LEU GLN GLY GLN MET VAL HIS GLN
SEQRES 2 R 231 ALA ILE SER CYS LEU CYS LEU ASN ALA TRP VAL LYS VAL
SEQRES 3 R 231 VAL GLU GLU LYS ALA PHE SER PRO GLU VAL ILE PRO MET
SEQRES 4 R 231 PHE SER ALA LEU SER GLU GLY ALA THR PRO GLN ASP LEU
SEQRES 5 R 231 ASN THR MET LEU ASN THR VAL GLY GLY HIS GLN ALA ALA
SEQRES 6 R 231 MET GLN MET LEU LYS GLU THR ILE ASN GLU GLU ALA ALA
SEQRES 7 R 231 GLU TRP ASP ARG LEU HIS PRO VAL HIS ALA GLY PRO ILE
SEQRES 8 R 231 ALA PRO GLY GLN MET ARG GLU PRO ARG GLY SER ASP ILE
SEQRES 9 R 231 ALA GLY THR THR SER THR LEU GLN GLU GLN ILE GLY TRP
SEQRES 10 R 231 MET THR HIS ASN PRO PRO ILE PRO VAL GLY GLU ILE TYR
SEQRES 11 R 231 LYS ARG TRP ILE ILE LEU GLY LEU ASN LYS ILE VAL ARG
SEQRES 12 R 231 MET TYR SER PRO THR SER ILE LEU ASP ILE ARG GLN GLY
SEQRES 13 R 231 PRO LYS GLU PRO PHE ARG ASP TYR VAL ASP ARG PHE TYR
SEQRES 14 R 231 LYS THR LEU ARG ALA GLU GLN ALA SER GLN GLU VAL LYS
SEQRES 15 R 231 ASN ALA ALA THR GLU THR LEU LEU VAL GLN ASN ALA ASN
SEQRES 16 R 231 PRO ASP CYS LYS THR ILE LEU LYS ALA LEU GLY PRO GLY
SEQRES 17 R 231 ALA THR LEU GLU GLU MET MET THR ALA CYS GLN GLY VAL
SEQRES 18 R 231 GLY GLY PRO GLY HIS LYS ALA ARG VAL LEU
SEQRES 1 S 231 PRO ILE VAL GLN ASN LEU GLN GLY GLN MET VAL HIS GLN
SEQRES 2 S 231 ALA ILE SER CYS LEU CYS LEU ASN ALA TRP VAL LYS VAL
SEQRES 3 S 231 VAL GLU GLU LYS ALA PHE SER PRO GLU VAL ILE PRO MET
SEQRES 4 S 231 PHE SER ALA LEU SER GLU GLY ALA THR PRO GLN ASP LEU
SEQRES 5 S 231 ASN THR MET LEU ASN THR VAL GLY GLY HIS GLN ALA ALA
SEQRES 6 S 231 MET GLN MET LEU LYS GLU THR ILE ASN GLU GLU ALA ALA
SEQRES 7 S 231 GLU TRP ASP ARG LEU HIS PRO VAL HIS ALA GLY PRO ILE
SEQRES 8 S 231 ALA PRO GLY GLN MET ARG GLU PRO ARG GLY SER ASP ILE
SEQRES 9 S 231 ALA GLY THR THR SER THR LEU GLN GLU GLN ILE GLY TRP
SEQRES 10 S 231 MET THR HIS ASN PRO PRO ILE PRO VAL GLY GLU ILE TYR
SEQRES 11 S 231 LYS ARG TRP ILE ILE LEU GLY LEU ASN LYS ILE VAL ARG
SEQRES 12 S 231 MET TYR SER PRO THR SER ILE LEU ASP ILE ARG GLN GLY
SEQRES 13 S 231 PRO LYS GLU PRO PHE ARG ASP TYR VAL ASP ARG PHE TYR
SEQRES 14 S 231 LYS THR LEU ARG ALA GLU GLN ALA SER GLN GLU VAL LYS
SEQRES 15 S 231 ASN ALA ALA THR GLU THR LEU LEU VAL GLN ASN ALA ASN
SEQRES 16 S 231 PRO ASP CYS LYS THR ILE LEU LYS ALA LEU GLY PRO GLY
SEQRES 17 S 231 ALA THR LEU GLU GLU MET MET THR ALA CYS GLN GLY VAL
SEQRES 18 S 231 GLY GLY PRO GLY HIS LYS ALA ARG VAL LEU
SEQRES 1 T 231 PRO ILE VAL GLN ASN LEU GLN GLY GLN MET VAL HIS GLN
SEQRES 2 T 231 ALA ILE SER CYS LEU CYS LEU ASN ALA TRP VAL LYS VAL
SEQRES 3 T 231 VAL GLU GLU LYS ALA PHE SER PRO GLU VAL ILE PRO MET
SEQRES 4 T 231 PHE SER ALA LEU SER GLU GLY ALA THR PRO GLN ASP LEU
SEQRES 5 T 231 ASN THR MET LEU ASN THR VAL GLY GLY HIS GLN ALA ALA
SEQRES 6 T 231 MET GLN MET LEU LYS GLU THR ILE ASN GLU GLU ALA ALA
SEQRES 7 T 231 GLU TRP ASP ARG LEU HIS PRO VAL HIS ALA GLY PRO ILE
SEQRES 8 T 231 ALA PRO GLY GLN MET ARG GLU PRO ARG GLY SER ASP ILE
SEQRES 9 T 231 ALA GLY THR THR SER THR LEU GLN GLU GLN ILE GLY TRP
SEQRES 10 T 231 MET THR HIS ASN PRO PRO ILE PRO VAL GLY GLU ILE TYR
SEQRES 11 T 231 LYS ARG TRP ILE ILE LEU GLY LEU ASN LYS ILE VAL ARG
SEQRES 12 T 231 MET TYR SER PRO THR SER ILE LEU ASP ILE ARG GLN GLY
SEQRES 13 T 231 PRO LYS GLU PRO PHE ARG ASP TYR VAL ASP ARG PHE TYR
SEQRES 14 T 231 LYS THR LEU ARG ALA GLU GLN ALA SER GLN GLU VAL LYS
SEQRES 15 T 231 ASN ALA ALA THR GLU THR LEU LEU VAL GLN ASN ALA ASN
SEQRES 16 T 231 PRO ASP CYS LYS THR ILE LEU LYS ALA LEU GLY PRO GLY
SEQRES 17 T 231 ALA THR LEU GLU GLU MET MET THR ALA CYS GLN GLY VAL
SEQRES 18 T 231 GLY GLY PRO GLY HIS LYS ALA ARG VAL LEU
CRYST1 86.794 122.128 149.304 74.41 74.36 81.48 P 1 20
ORIGX1 1.000000 0.000000 0.000000 0.00000
ORIGX2 0.000000 1.000000 0.000000 0.00000
ORIGX3 0.000000 0.000000 1.000000 0.00000
SCALE1 0.011522 -0.001726 -0.002896 0.00000
SCALE2 0.000000 0.008280 -0.002049 0.00000
SCALE3 0.000000 0.000000 0.007165 0.00000
(ATOM LINES ARE NOT SHOWN.)
END
