GenomeNet

Database: PDB
Entry: 3P35
LinkDB: 3P35
Original site: 3P35 
HEADER    TRANSFERASE                             04-OCT-10   3P35              
TITLE     POLO-LIKE KINASE I POLO-BOX DOMAIN IN COMPLEX WITH MQSPSPL            
TITLE    2 PHOSPHOPEPTIDE                                                       
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: SERINE/THREONINE-PROTEIN KINASE PLK1;                      
COMPND   3 CHAIN: A, B, C;                                                      
COMPND   4 FRAGMENT: POLO-BOX DOMAIN;                                           
COMPND   5 SYNONYM: POLO-LIKE KINASE 1, PLK-1, SERINE/THREONINE-PROTEIN KINASE  
COMPND   6 13, STPK13;                                                          
COMPND   7 EC: 2.7.11.21;                                                       
COMPND   8 ENGINEERED: YES;                                                     
COMPND   9 MOL_ID: 2;                                                           
COMPND  10 MOLECULE: PHOSPHOPEPTIDE;                                            
COMPND  11 CHAIN: D, E;                                                         
COMPND  12 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: PLK1, PLK;                                                     
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE   8 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE   9 EXPRESSION_SYSTEM_PLASMID: PGEX-6P-1;                                
SOURCE  10 MOL_ID: 2;                                                           
SOURCE  11 SYNTHETIC: YES;                                                      
SOURCE  12 OTHER_DETAILS: CHEMICALLY SYNTHESIZED                                
KEYWDS    PHOSPHOPROTEIN BINDING DOMAIN, PLK1, TRANSFERASE                      
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    P.SLEDZ,M.HYVONEN,C.ABELL                                             
REVDAT   1   27-APR-11 3P35    0                                                
JRNL        AUTH   P.SLEDZ,C.J.STUBBS,S.LANG,Y.Q.YANG,G.J.MCKENZIE,             
JRNL        AUTH 2 A.R.VENKITARAMAN,M.HYVONEN,C.ABELL                           
JRNL        TITL   FROM CRYSTAL PACKING TO MOLECULAR RECOGNITION: PREDICTION    
JRNL        TITL 2 AND DISCOVERY OF A BINDING SITE ON THE SURFACE OF POLO-LIKE  
JRNL        TITL 3 KINASE 1                                                     
JRNL        REF    ANGEW.CHEM.INT.ED.ENGL.       V.  50  4003 2011              
JRNL        REFN                   ISSN 1433-7851                               
JRNL        PMID   21472932                                                     
JRNL        DOI    10.1002/ANIE.201008019                                       
REMARK   2                                                                      
REMARK   2 RESOLUTION.    2.09 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : REFMAC 5.5.0109                                      
REMARK   3   AUTHORS     : MURSHUDOV,VAGIN,DODSON                               
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : MAXIMUM LIKELIHOOD                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.09                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 59.13                          
REMARK   3   DATA CUTOFF            (SIGMA(F)) : NULL                           
REMARK   3   COMPLETENESS FOR RANGE        (%) : 98.5                           
REMARK   3   NUMBER OF REFLECTIONS             : 36378                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   CROSS-VALIDATION METHOD          : THROUGHOUT                      
REMARK   3   FREE R VALUE TEST SET SELECTION  : RANDOM                          
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.209                           
REMARK   3   R VALUE            (WORKING SET) : 0.206                           
REMARK   3   FREE R VALUE                     : 0.273                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.000                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 1921                            
REMARK   3                                                                      
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.                                  
REMARK   3   TOTAL NUMBER OF BINS USED           : 20                           
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : 2.09                         
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : 2.14                         
REMARK   3   REFLECTION IN BIN     (WORKING SET) : 2605                         
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : 96.51                        
REMARK   3   BIN R VALUE           (WORKING SET) : 0.2430                       
REMARK   3   BIN FREE R VALUE SET COUNT          : 135                          
REMARK   3   BIN FREE R VALUE                    : 0.3290                       
REMARK   3                                                                      
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.                    
REMARK   3   PROTEIN ATOMS            : 5452                                    
REMARK   3   NUCLEIC ACID ATOMS       : 0                                       
REMARK   3   HETEROGEN ATOMS          : 30                                      
REMARK   3   SOLVENT ATOMS            : 486                                     
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 18.92                          
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  ESTIMATED OVERALL COORDINATE ERROR.                                 
REMARK   3   ESU BASED ON R VALUE                            (A): NULL          
REMARK   3   ESU BASED ON FREE R VALUE                       (A): 0.238         
REMARK   3   ESU BASED ON MAXIMUM LIKELIHOOD                 (A): 0.177         
REMARK   3   ESU FOR B VALUES BASED ON MAXIMUM LIKELIHOOD (A**2): 13.586        
REMARK   3                                                                      
REMARK   3 CORRELATION COEFFICIENTS.                                            
REMARK   3   CORRELATION COEFFICIENT FO-FC      : 0.932                         
REMARK   3   CORRELATION COEFFICIENT FO-FC FREE : 0.884                         
REMARK   3                                                                      
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES        COUNT    RMS    WEIGHT      
REMARK   3   BOND LENGTHS REFINED ATOMS        (A):  5697 ; 0.006 ; 0.022       
REMARK   3   BOND LENGTHS OTHERS               (A):  NULL ;  NULL ;  NULL       
REMARK   3   BOND ANGLES REFINED ATOMS   (DEGREES):  7723 ; 1.020 ; 1.974       
REMARK   3   BOND ANGLES OTHERS          (DEGREES):  NULL ;  NULL ;  NULL       
REMARK   3   TORSION ANGLES, PERIOD 1    (DEGREES):   701 ; 5.312 ; 5.000       
REMARK   3   TORSION ANGLES, PERIOD 2    (DEGREES):   267 ;30.673 ;22.996       
REMARK   3   TORSION ANGLES, PERIOD 3    (DEGREES):  1022 ;16.853 ;15.029       
REMARK   3   TORSION ANGLES, PERIOD 4    (DEGREES):    49 ;10.551 ;15.000       
REMARK   3   CHIRAL-CENTER RESTRAINTS       (A**3):   853 ; 0.070 ; 0.200       
REMARK   3   GENERAL PLANES REFINED ATOMS      (A):  4266 ; 0.003 ; 0.021       
REMARK   3   GENERAL PLANES OTHERS             (A):  NULL ;  NULL ;  NULL       
REMARK   3   NON-BONDED CONTACTS REFINED ATOMS (A):  NULL ;  NULL ;  NULL       
REMARK   3   NON-BONDED CONTACTS OTHERS        (A):  NULL ;  NULL ;  NULL       
REMARK   3   NON-BONDED TORSION REFINED ATOMS  (A):  NULL ;  NULL ;  NULL       
REMARK   3   NON-BONDED TORSION OTHERS         (A):  NULL ;  NULL ;  NULL       
REMARK   3   H-BOND (X...Y) REFINED ATOMS      (A):  NULL ;  NULL ;  NULL       
REMARK   3   H-BOND (X...Y) OTHERS             (A):  NULL ;  NULL ;  NULL       
REMARK   3   POTENTIAL METAL-ION REFINED ATOMS (A):  NULL ;  NULL ;  NULL       
REMARK   3   POTENTIAL METAL-ION OTHERS        (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY VDW REFINED ATOMS        (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY VDW OTHERS               (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY H-BOND REFINED ATOMS     (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY H-BOND OTHERS            (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY METAL-ION REFINED ATOMS  (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY METAL-ION OTHERS         (A):  NULL ;  NULL ;  NULL       
REMARK   3                                                                      
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.     COUNT   RMS    WEIGHT      
REMARK   3   MAIN-CHAIN BOND REFINED ATOMS  (A**2):  3416 ; 0.313 ; 1.500       
REMARK   3   MAIN-CHAIN BOND OTHER ATOMS    (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   MAIN-CHAIN ANGLE REFINED ATOMS (A**2):  5528 ; 0.610 ; 2.000       
REMARK   3   SIDE-CHAIN BOND REFINED ATOMS  (A**2):  2281 ; 0.869 ; 3.000       
REMARK   3   SIDE-CHAIN ANGLE REFINED ATOMS (A**2):  2179 ; 1.452 ; 4.500       
REMARK   3                                                                      
REMARK   3 ANISOTROPIC THERMAL FACTOR RESTRAINTS.    COUNT   RMS   WEIGHT       
REMARK   3   RIGID-BOND RESTRAINTS          (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   SPHERICITY; FREE ATOMS         (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   SPHERICITY; BONDED ATOMS       (A**2):  NULL ;  NULL ;  NULL       
REMARK   3                                                                      
REMARK   3  NCS RESTRAINTS STATISTICS                                           
REMARK   3   NUMBER OF DIFFERENT NCS GROUPS : NULL                              
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : 3                                          
REMARK   3                                                                      
REMARK   3   TLS GROUP : 1                                                      
REMARK   3    NUMBER OF COMPONENTS GROUP : 1                                    
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI                         
REMARK   3    RESIDUE RANGE :   A   375        A   590                          
REMARK   3    ORIGIN FOR THE GROUP (A):   8.3430   9.3220  28.6290              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.0221 T22:   0.0843                                     
REMARK   3      T33:   0.0194 T12:   0.0123                                     
REMARK   3      T13:   0.0003 T23:   0.0320                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.6824 L22:   1.8763                                     
REMARK   3      L33:   1.3795 L12:   0.0936                                     
REMARK   3      L13:  -0.0136 L23:   0.9423                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0390 S12:  -0.0257 S13:  -0.0063                       
REMARK   3      S21:   0.0570 S22:  -0.0597 S23:   0.0564                       
REMARK   3      S31:  -0.0008 S32:  -0.0270 S33:   0.0207                       
REMARK   3                                                                      
REMARK   3   TLS GROUP : 2                                                      
REMARK   3    NUMBER OF COMPONENTS GROUP : 1                                    
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI                         
REMARK   3    RESIDUE RANGE :   B   375        B   590                          
REMARK   3    ORIGIN FOR THE GROUP (A):  37.2380   6.9340  47.0240              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.0064 T22:   0.0653                                     
REMARK   3      T33:   0.0119 T12:  -0.0003                                     
REMARK   3      T13:  -0.0011 T23:   0.0265                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.9566 L22:   1.7914                                     
REMARK   3      L33:   2.4082 L12:   0.3267                                     
REMARK   3      L13:   0.2127 L23:   1.1399                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0032 S12:  -0.0582 S13:  -0.0446                       
REMARK   3      S21:   0.0654 S22:   0.0097 S23:  -0.0112                       
REMARK   3      S31:   0.0514 S32:   0.0677 S33:  -0.0129                       
REMARK   3                                                                      
REMARK   3   TLS GROUP : 3                                                      
REMARK   3    NUMBER OF COMPONENTS GROUP : 1                                    
REMARK   3    COMPONENTS        C SSSEQI   TO  C SSSEQI                         
REMARK   3    RESIDUE RANGE :   C   375        C   590                          
REMARK   3    ORIGIN FOR THE GROUP (A):   7.5020   6.3790  66.3510              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.0205 T22:   0.0742                                     
REMARK   3      T33:   0.0058 T12:   0.0025                                     
REMARK   3      T13:   0.0005 T23:   0.0190                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.8755 L22:   2.2341                                     
REMARK   3      L33:   1.1073 L12:   0.1578                                     
REMARK   3      L13:   0.2461 L23:   0.6934                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0085 S12:  -0.0106 S13:   0.0038                       
REMARK   3      S21:   0.0169 S22:  -0.0008 S23:  -0.0433                       
REMARK   3      S31:  -0.0184 S32:   0.0167 S33:  -0.0076                       
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED : MASK                                                 
REMARK   3   PARAMETERS FOR MASK CALCULATION                                    
REMARK   3   VDW PROBE RADIUS   : 1.40                                          
REMARK   3   ION PROBE RADIUS   : 0.80                                          
REMARK   3   SHRINKAGE RADIUS   : 0.80                                          
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 3P35 COMPLIES WITH FORMAT V. 3.20, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 20-OCT-10.                  
REMARK 100 THE RCSB ID CODE IS RCSB061896.                                      
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 22-APR-10                          
REMARK 200  TEMPERATURE           (KELVIN) : NULL                               
REMARK 200  PH                             : NULL                               
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : ESRF                               
REMARK 200  BEAMLINE                       : ID14-1                             
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.9334                             
REMARK 200  MONOCHROMATOR                  : NULL                               
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : NULL                               
REMARK 200  DETECTOR MANUFACTURER          : NULL                               
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : NULL                               
REMARK 200  DATA SCALING SOFTWARE          : NULL                               
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 38299                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.090                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 59.130                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 98.1                               
REMARK 200  DATA REDUNDANCY                : NULL                               
REMARK 200  R MERGE                    (I) : NULL                               
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : NULL                               
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : NULL                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : NULL                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : NULL                               
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL                               
REMARK 200  R MERGE FOR SHELL          (I) : NULL                               
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : NULL                               
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: PHASER                                                
REMARK 200 STARTING MODEL: 1UMW (CHAIN A)                                       
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 41.42                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.10                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.2M POTASSIUM FORMATE, 20% PEG 3350,    
REMARK 280  VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K                     
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 1 21 1                         
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,Y+1/2,-Z                                             
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000  1.000000  0.000000       47.91400            
REMARK 290   SMTRY3   2  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1, 2, 3                                                 
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC                    
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 1390 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 11140 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -10.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, D                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 2                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC                    
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 1340 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 11690 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -10.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: B, E                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 3                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC                         
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: MONOMERIC                  
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 APPLY THE FOLLOWING TO CHAINS: C                                     
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     GLY A     1                                                      
REMARK 465     PRO A     2                                                      
REMARK 465     LEU A     3                                                      
REMARK 465     GLY A     4                                                      
REMARK 465     SER A     5                                                      
REMARK 465     PRO A     6                                                      
REMARK 465     GLU A     7                                                      
REMARK 465     PHE A     8                                                      
REMARK 465     ASP A   371                                                      
REMARK 465     GLU A   501                                                      
REMARK 465     GLY A   502                                                      
REMARK 465     ASP A   503                                                      
REMARK 465     GLU A   504                                                      
REMARK 465     LEU A   505                                                      
REMARK 465     ALA A   506                                                      
REMARK 465     GLY B     1                                                      
REMARK 465     PRO B     2                                                      
REMARK 465     LEU B     3                                                      
REMARK 465     GLY B     4                                                      
REMARK 465     SER B     5                                                      
REMARK 465     PRO B     6                                                      
REMARK 465     GLU B     7                                                      
REMARK 465     PHE B     8                                                      
REMARK 465     ASP B   371                                                      
REMARK 465     ARG B   594                                                      
REMARK 465     GLY C     1                                                      
REMARK 465     PRO C     2                                                      
REMARK 465     LEU C     3                                                      
REMARK 465     GLY C     4                                                      
REMARK 465     SER C     5                                                      
REMARK 465     PRO C     6                                                      
REMARK 465     GLU C     7                                                      
REMARK 465     PHE C     8                                                      
REMARK 465     ASP C   371                                                      
REMARK 465     ARG C   594                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     CYS A 372    SG                                                  
REMARK 470     ARG A 512    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     GLU A 555    CG   CD   OE1  OE2                                  
REMARK 470     ARG A 594    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ARG B 456    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ASP B 503    CG   OD1  OD2                                       
REMARK 470     GLU C 555    CG   CD   OE1  OE2                                  
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    GLU A 391       54.18    -96.77                                   
REMARK 500    LYS A 420      -40.29   -139.02                                   
REMARK 500    ASP A 449      -39.13   -142.67                                   
REMARK 500    ARG B 396       52.76   -118.66                                   
REMARK 500    ASN B 430       -1.15     84.80                                   
REMARK 500    ASP B 449      -43.61   -138.80                                   
REMARK 500    TYR C 417       50.40   -119.96                                   
REMARK 500    ASN C 430        4.34     80.50                                   
REMARK 500    ASP C 449      -43.24   -139.36                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 525                                                                      
REMARK 525 SOLVENT                                                              
REMARK 525                                                                      
REMARK 525 THE SOLVENT MOLECULES HAVE CHAIN IDENTIFIERS THAT                    
REMARK 525 INDICATE THE POLYMER CHAIN WITH WHICH THEY ARE MOST                  
REMARK 525 CLOSELY ASSOCIATED. THE REMARK LISTS ALL THE SOLVENT                 
REMARK 525 MOLECULES WHICH ARE MORE THAN 5A AWAY FROM THE                       
REMARK 525 NEAREST POLYMER CHAIN (M = MODEL NUMBER;                             
REMARK 525 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE                  
REMARK 525 NUMBER; I=INSERTION CODE):                                           
REMARK 525                                                                      
REMARK 525  M RES CSSEQI                                                        
REMARK 525    HOH C  41        DISTANCE =  5.82 ANGSTROMS                       
REMARK 525    HOH C 339        DISTANCE =  5.27 ANGSTROMS                       
REMARK 800                                                                      
REMARK 800 SITE                                                                 
REMARK 800 SITE_IDENTIFIER: AC1                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE GOL A 595                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC2                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE GOL A 596                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC3                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE GOL B 595                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC4                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE GOL B 596                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC5                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE GOL B 597                 
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 3P2W   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 3P2Z   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 3P34   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 3P36   RELATED DB: PDB                                   
REMARK 900 RELATED ID: 3P37   RELATED DB: PDB                                   
DBREF  3P35 A  371   594  UNP    P53350   PLK1_HUMAN     371    594             
DBREF  3P35 B  371   594  UNP    P53350   PLK1_HUMAN     371    594             
DBREF  3P35 C  371   594  UNP    P53350   PLK1_HUMAN     371    594             
DBREF  3P35 D    0     7  PDB    3P35     3P35             0      7             
DBREF  3P35 E    0     7  PDB    3P35     3P35             0      7             
SEQADV 3P35 GLY A    1  UNP  P53350              EXPRESSION TAG                 
SEQADV 3P35 PRO A    2  UNP  P53350              EXPRESSION TAG                 
SEQADV 3P35 LEU A    3  UNP  P53350              EXPRESSION TAG                 
SEQADV 3P35 GLY A    4  UNP  P53350              EXPRESSION TAG                 
SEQADV 3P35 SER A    5  UNP  P53350              EXPRESSION TAG                 
SEQADV 3P35 PRO A    6  UNP  P53350              EXPRESSION TAG                 
SEQADV 3P35 GLU A    7  UNP  P53350              EXPRESSION TAG                 
SEQADV 3P35 PHE A    8  UNP  P53350              EXPRESSION TAG                 
SEQADV 3P35 GLY B    1  UNP  P53350              EXPRESSION TAG                 
SEQADV 3P35 PRO B    2  UNP  P53350              EXPRESSION TAG                 
SEQADV 3P35 LEU B    3  UNP  P53350              EXPRESSION TAG                 
SEQADV 3P35 GLY B    4  UNP  P53350              EXPRESSION TAG                 
SEQADV 3P35 SER B    5  UNP  P53350              EXPRESSION TAG                 
SEQADV 3P35 PRO B    6  UNP  P53350              EXPRESSION TAG                 
SEQADV 3P35 GLU B    7  UNP  P53350              EXPRESSION TAG                 
SEQADV 3P35 PHE B    8  UNP  P53350              EXPRESSION TAG                 
SEQADV 3P35 GLY C    1  UNP  P53350              EXPRESSION TAG                 
SEQADV 3P35 PRO C    2  UNP  P53350              EXPRESSION TAG                 
SEQADV 3P35 LEU C    3  UNP  P53350              EXPRESSION TAG                 
SEQADV 3P35 GLY C    4  UNP  P53350              EXPRESSION TAG                 
SEQADV 3P35 SER C    5  UNP  P53350              EXPRESSION TAG                 
SEQADV 3P35 PRO C    6  UNP  P53350              EXPRESSION TAG                 
SEQADV 3P35 GLU C    7  UNP  P53350              EXPRESSION TAG                 
SEQADV 3P35 PHE C    8  UNP  P53350              EXPRESSION TAG                 
SEQRES   1 A  232  GLY PRO LEU GLY SER PRO GLU PHE ASP CYS HIS LEU SER          
SEQRES   2 A  232  ASP MET LEU GLN GLN LEU HIS SER VAL ASN ALA SER LYS          
SEQRES   3 A  232  PRO SER GLU ARG GLY LEU VAL ARG GLN GLU GLU ALA GLU          
SEQRES   4 A  232  ASP PRO ALA CYS ILE PRO ILE PHE TRP VAL SER LYS TRP          
SEQRES   5 A  232  VAL ASP TYR SER ASP LYS TYR GLY LEU GLY TYR GLN LEU          
SEQRES   6 A  232  CYS ASP ASN SER VAL GLY VAL LEU PHE ASN ASP SER THR          
SEQRES   7 A  232  ARG LEU ILE LEU TYR ASN ASP GLY ASP SER LEU GLN TYR          
SEQRES   8 A  232  ILE GLU ARG ASP GLY THR GLU SER TYR LEU THR VAL SER          
SEQRES   9 A  232  SER HIS PRO ASN SER LEU MET LYS LYS ILE THR LEU LEU          
SEQRES  10 A  232  LYS TYR PHE ARG ASN TYR MET SER GLU HIS LEU LEU LYS          
SEQRES  11 A  232  ALA GLY ALA ASN ILE THR PRO ARG GLU GLY ASP GLU LEU          
SEQRES  12 A  232  ALA ARG LEU PRO TYR LEU ARG THR TRP PHE ARG THR ARG          
SEQRES  13 A  232  SER ALA ILE ILE LEU HIS LEU SER ASN GLY SER VAL GLN          
SEQRES  14 A  232  ILE ASN PHE PHE GLN ASP HIS THR LYS LEU ILE LEU CYS          
SEQRES  15 A  232  PRO LEU MET ALA ALA VAL THR TYR ILE ASP GLU LYS ARG          
SEQRES  16 A  232  ASP PHE ARG THR TYR ARG LEU SER LEU LEU GLU GLU TYR          
SEQRES  17 A  232  GLY CYS CYS LYS GLU LEU ALA SER ARG LEU ARG TYR ALA          
SEQRES  18 A  232  ARG THR MET VAL ASP LYS LEU LEU SER SER ARG                  
SEQRES   1 B  232  GLY PRO LEU GLY SER PRO GLU PHE ASP CYS HIS LEU SER          
SEQRES   2 B  232  ASP MET LEU GLN GLN LEU HIS SER VAL ASN ALA SER LYS          
SEQRES   3 B  232  PRO SER GLU ARG GLY LEU VAL ARG GLN GLU GLU ALA GLU          
SEQRES   4 B  232  ASP PRO ALA CYS ILE PRO ILE PHE TRP VAL SER LYS TRP          
SEQRES   5 B  232  VAL ASP TYR SER ASP LYS TYR GLY LEU GLY TYR GLN LEU          
SEQRES   6 B  232  CYS ASP ASN SER VAL GLY VAL LEU PHE ASN ASP SER THR          
SEQRES   7 B  232  ARG LEU ILE LEU TYR ASN ASP GLY ASP SER LEU GLN TYR          
SEQRES   8 B  232  ILE GLU ARG ASP GLY THR GLU SER TYR LEU THR VAL SER          
SEQRES   9 B  232  SER HIS PRO ASN SER LEU MET LYS LYS ILE THR LEU LEU          
SEQRES  10 B  232  LYS TYR PHE ARG ASN TYR MET SER GLU HIS LEU LEU LYS          
SEQRES  11 B  232  ALA GLY ALA ASN ILE THR PRO ARG GLU GLY ASP GLU LEU          
SEQRES  12 B  232  ALA ARG LEU PRO TYR LEU ARG THR TRP PHE ARG THR ARG          
SEQRES  13 B  232  SER ALA ILE ILE LEU HIS LEU SER ASN GLY SER VAL GLN          
SEQRES  14 B  232  ILE ASN PHE PHE GLN ASP HIS THR LYS LEU ILE LEU CYS          
SEQRES  15 B  232  PRO LEU MET ALA ALA VAL THR TYR ILE ASP GLU LYS ARG          
SEQRES  16 B  232  ASP PHE ARG THR TYR ARG LEU SER LEU LEU GLU GLU TYR          
SEQRES  17 B  232  GLY CYS CYS LYS GLU LEU ALA SER ARG LEU ARG TYR ALA          
SEQRES  18 B  232  ARG THR MET VAL ASP LYS LEU LEU SER SER ARG                  
SEQRES   1 C  232  GLY PRO LEU GLY SER PRO GLU PHE ASP CYS HIS LEU SER          
SEQRES   2 C  232  ASP MET LEU GLN GLN LEU HIS SER VAL ASN ALA SER LYS          
SEQRES   3 C  232  PRO SER GLU ARG GLY LEU VAL ARG GLN GLU GLU ALA GLU          
SEQRES   4 C  232  ASP PRO ALA CYS ILE PRO ILE PHE TRP VAL SER LYS TRP          
SEQRES   5 C  232  VAL ASP TYR SER ASP LYS TYR GLY LEU GLY TYR GLN LEU          
SEQRES   6 C  232  CYS ASP ASN SER VAL GLY VAL LEU PHE ASN ASP SER THR          
SEQRES   7 C  232  ARG LEU ILE LEU TYR ASN ASP GLY ASP SER LEU GLN TYR          
SEQRES   8 C  232  ILE GLU ARG ASP GLY THR GLU SER TYR LEU THR VAL SER          
SEQRES   9 C  232  SER HIS PRO ASN SER LEU MET LYS LYS ILE THR LEU LEU          
SEQRES  10 C  232  LYS TYR PHE ARG ASN TYR MET SER GLU HIS LEU LEU LYS          
SEQRES  11 C  232  ALA GLY ALA ASN ILE THR PRO ARG GLU GLY ASP GLU LEU          
SEQRES  12 C  232  ALA ARG LEU PRO TYR LEU ARG THR TRP PHE ARG THR ARG          
SEQRES  13 C  232  SER ALA ILE ILE LEU HIS LEU SER ASN GLY SER VAL GLN          
SEQRES  14 C  232  ILE ASN PHE PHE GLN ASP HIS THR LYS LEU ILE LEU CYS          
SEQRES  15 C  232  PRO LEU MET ALA ALA VAL THR TYR ILE ASP GLU LYS ARG          
SEQRES  16 C  232  ASP PHE ARG THR TYR ARG LEU SER LEU LEU GLU GLU TYR          
SEQRES  17 C  232  GLY CYS CYS LYS GLU LEU ALA SER ARG LEU ARG TYR ALA          
SEQRES  18 C  232  ARG THR MET VAL ASP LYS LEU LEU SER SER ARG                  
SEQRES   1 D    8  ACE MET GLN SER SEP PRO LEU NH2                              
SEQRES   1 E    8  ACE MET GLN SER SEP PRO LEU NH2                              
MODRES 3P35 SEP D    4  SER  PHOSPHOSERINE                                      
MODRES 3P35 SEP E    4  SER  PHOSPHOSERINE                                      
HET    ACE  D   0       3                                                       
HET    SEP  D   4      10                                                       
HET    NH2  D   7       1                                                       
HET    ACE  E   0       3                                                       
HET    SEP  E   4      10                                                       
HET    NH2  E   7       1                                                       
HET    GOL  A 595       6                                                       
HET    GOL  A 596       6                                                       
HET    GOL  B 595       6                                                       
HET    GOL  B 596       6                                                       
HET    GOL  B 597       6                                                       
HETNAM     ACE ACETYL GROUP                                                     
HETNAM     SEP PHOSPHOSERINE                                                    
HETNAM     NH2 AMINO GROUP                                                      
HETNAM     GOL GLYCEROL                                                         
HETSYN     SEP PHOSPHONOSERINE                                                  
HETSYN     GOL GLYCERIN; PROPANE-1,2,3-TRIOL                                    
FORMUL   4  ACE    2(C2 H4 O)                                                   
FORMUL   4  SEP    2(C3 H8 N O6 P)                                              
FORMUL   4  NH2    2(H2 N)                                                      
FORMUL   6  GOL    5(C3 H8 O3)                                                  
FORMUL  11  HOH   *486(H2 O)                                                    
HELIX    1   1 HIS A  373  SER A  387  1                                  15    
HELIX    2   2 ARG A  396  GLU A  401  5                                   6    
HELIX    3   3 ASP A  402  ILE A  406  5                                   5    
HELIX    4   4 PRO A  469  SER A  471  5                                   3    
HELIX    5   5 LEU A  472  LEU A  490  1                                  19    
HELIX    6   6 LEU A  564  GLY A  571  1                                   8    
HELIX    7   7 CYS A  573  SER A  593  1                                  21    
HELIX    8   8 CYS B  372  SER B  387  1                                  16    
HELIX    9   9 ARG B  396  GLU B  401  5                                   6    
HELIX   10  10 ASP B  402  ILE B  406  5                                   5    
HELIX   11  11 PRO B  469  SER B  471  5                                   3    
HELIX   12  12 LEU B  472  LEU B  490  1                                  19    
HELIX   13  13 LEU B  564  GLY B  571  1                                   8    
HELIX   14  14 CYS B  573  SER B  593  1                                  21    
HELIX   15  15 CYS C  372  SER C  387  1                                  16    
HELIX   16  16 ARG C  396  GLU C  401  5                                   6    
HELIX   17  17 ASP C  402  ILE C  406  5                                   5    
HELIX   18  18 PRO C  469  SER C  471  5                                   3    
HELIX   19  19 LEU C  472  LEU C  490  1                                  19    
HELIX   20  20 LEU C  564  GLY C  571  1                                   8    
HELIX   21  21 CYS C  573  SER C  593  1                                  21    
SHEET    1   A 7 GLU A 460  THR A 464  0                                        
SHEET    2   A 7 SER A 450  ILE A 454 -1  N  LEU A 451   O  LEU A 463           
SHEET    3   A 7 ARG A 441  LEU A 444 -1  N  ARG A 441   O  ILE A 454           
SHEET    4   A 7 VAL A 432  LEU A 435 -1  N  VAL A 432   O  LEU A 444           
SHEET    5   A 7 GLY A 422  LEU A 427 -1  N  LEU A 423   O  LEU A 435           
SHEET    6   A 7 VAL A 411  TYR A 417 -1  N  LYS A 413   O  GLN A 426           
SHEET    7   A 7 MET D   1  GLN D   2 -1  O  MET D   1   N  ASP A 416           
SHEET    1   B 6 LEU A 511  ARG A 516  0                                        
SHEET    2   B 6 ALA A 520  LEU A 525 -1  O  HIS A 524   N  THR A 513           
SHEET    3   B 6 VAL A 530  PHE A 534 -1  O  GLN A 531   N  LEU A 523           
SHEET    4   B 6 LYS A 540  CYS A 544 -1  O  LEU A 543   N  VAL A 530           
SHEET    5   B 6 ALA A 549  ILE A 553 -1  O  THR A 551   N  ILE A 542           
SHEET    6   B 6 PHE A 559  ARG A 563 -1  O  TYR A 562   N  VAL A 550           
SHEET    1   C 6 VAL B 411  ASP B 416  0                                        
SHEET    2   C 6 GLY B 422  LEU B 427 -1  O  GLY B 424   N  VAL B 415           
SHEET    3   C 6 VAL B 432  PHE B 436 -1  O  LEU B 435   N  LEU B 423           
SHEET    4   C 6 ARG B 441  LEU B 444 -1  O  LEU B 444   N  VAL B 432           
SHEET    5   C 6 SER B 450  ILE B 454 -1  O  ILE B 454   N  ARG B 441           
SHEET    6   C 6 GLU B 460  THR B 464 -1  O  LEU B 463   N  LEU B 451           
SHEET    1   D 6 LEU B 511  ARG B 516  0                                        
SHEET    2   D 6 ALA B 520  LEU B 525 -1  O  ILE B 522   N  PHE B 515           
SHEET    3   D 6 VAL B 530  PHE B 534 -1  O  GLN B 531   N  LEU B 523           
SHEET    4   D 6 LYS B 540  CYS B 544 -1  O  LEU B 543   N  VAL B 530           
SHEET    5   D 6 ALA B 549  ILE B 553 -1  O  ILE B 553   N  LYS B 540           
SHEET    6   D 6 PHE B 559  ARG B 563 -1  O  TYR B 562   N  VAL B 550           
SHEET    1   E 6 VAL C 411  TYR C 417  0                                        
SHEET    2   E 6 GLY C 422  LEU C 427 -1  O  GLY C 424   N  VAL C 415           
SHEET    3   E 6 VAL C 432  PHE C 436 -1  O  GLY C 433   N  TYR C 425           
SHEET    4   E 6 ARG C 441  LEU C 444 -1  O  LEU C 444   N  VAL C 432           
SHEET    5   E 6 SER C 450  ILE C 454 -1  O  ILE C 454   N  ARG C 441           
SHEET    6   E 6 GLU C 460  THR C 464 -1  O  LEU C 463   N  LEU C 451           
SHEET    1   F 6 LEU C 511  ARG C 516  0                                        
SHEET    2   F 6 ALA C 520  LEU C 525 -1  O  ILE C 522   N  PHE C 515           
SHEET    3   F 6 VAL C 530  PHE C 534 -1  O  GLN C 531   N  LEU C 523           
SHEET    4   F 6 LYS C 540  CYS C 544 -1  O  LEU C 541   N  ILE C 532           
SHEET    5   F 6 ALA C 549  ILE C 553 -1  O  THR C 551   N  ILE C 542           
SHEET    6   F 6 PHE C 559  ARG C 563 -1  O  TYR C 562   N  VAL C 550           
LINK         C   ACE D   0                 N   MET D   1     1555   1555  1.34  
LINK         C   SER D   3                 N   SEP D   4     1555   1555  1.33  
LINK         C   SEP D   4                 N   PRO D   5     1555   1555  1.35  
LINK         C   LEU D   6                 N   NH2 D   7     1555   1555  1.33  
LINK         C   ACE E   0                 N   MET E   1     1555   1555  1.33  
LINK         C   SER E   3                 N   SEP E   4     1555   1555  1.33  
LINK         C   SEP E   4                 N   PRO E   5     1555   1555  1.35  
LINK         C   LEU E   6                 N   NH2 E   7     1555   1555  1.33  
SITE     1 AC1  5 ASN A 385  PRO A 389  ALA A 577  ARG A 581                    
SITE     2 AC1  5 GLU B 504                                                     
SITE     1 AC2  7 ARG A 507  LEU A 508  TYR A 510  SER A 526                    
SITE     2 AC2  7 GLN B 536  ASP B 537  HIS B 538                               
SITE     1 AC3  6 THR B 459  GLU B 460  HOH B 601  HOH C  10                    
SITE     2 AC3  6 TRP C 414  LEU C 490                                          
SITE     1 AC4  2 LEU B 566  GLU B 569                                          
SITE     1 AC5  6 TYR B 421  PHE B 436  ASN B 437  ASP B 438                    
SITE     2 AC5  6 LYS B 475  HOH B 598                                          
CRYST1   58.893   95.828   65.964  90.00 116.34  90.00 P 1 21 1      6          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.016980  0.000000  0.008408        0.00000                         
SCALE2      0.000000  0.010435  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.016916        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
DBGET integrated database retrieval system