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Database: PDB
Entry: 4ERV
LinkDB: 4ERV
Original site: 4ERV 
HEADER    METAL TRANSPORT                         20-APR-12   4ERV              
TITLE     CRYSTAL STRUCTURE OF HUMAN RYANODINE RECEPTOR 3 (2597-2800)           
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: RYANODINE RECEPTOR 3;                                      
COMPND   3 CHAIN: A;                                                            
COMPND   4 SYNONYM: RYR-3, RYR3, BRAIN RYANODINE RECEPTOR-CALCIUM RELEASE       
COMPND   5 CHANNEL, BRAIN-TYPE RYANODINE RECEPTOR, TYPE 3 RYANODINE RECEPTOR;   
COMPND   6 ENGINEERED: YES;                                                     
COMPND   7 MUTATION: YES                                                        
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: RYR3, HBRR;                                                    
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE   8 EXPRESSION_SYSTEM_STRAIN: ROSETTA(DE3)PLACI;                         
SOURCE   9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  10 EXPRESSION_SYSTEM_PLASMID: PET28HMT                                  
KEYWDS    RYANODINE RECEPTOR CALCIUM RELEASE CHANNEL, METAL TRANSPORT           
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    Z.YUCHI,K.LAU,F.VAN PETEGEM                                           
REVDAT   2   17-OCT-12 4ERV    1       JRNL                                     
REVDAT   1   13-JUN-12 4ERV    0                                                
JRNL        AUTH   Z.YUCHI,K.LAU,F.VAN PETEGEM                                  
JRNL        TITL   DISEASE MUTATIONS IN THE RYANODINE RECEPTOR CENTRAL REGION:  
JRNL        TITL 2 CRYSTAL STRUCTURES OF A PHOSPHORYLATION HOT SPOT DOMAIN.     
JRNL        REF    STRUCTURE                     V.  20  1201 2012              
JRNL        REFN                   ISSN 0969-2126                               
JRNL        PMID   22705209                                                     
JRNL        DOI    10.1016/J.STR.2012.04.015                                    
REMARK   2                                                                      
REMARK   2 RESOLUTION.    1.75 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : REFMAC 5.6.0117                                      
REMARK   3   AUTHORS     : MURSHUDOV,VAGIN,DODSON                               
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : MAXIMUM LIKELIHOOD                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.75                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 35.35                          
REMARK   3   DATA CUTOFF            (SIGMA(F)) : 0.000                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 98.1                           
REMARK   3   NUMBER OF REFLECTIONS             : 25283                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   CROSS-VALIDATION METHOD          : THROUGHOUT                      
REMARK   3   FREE R VALUE TEST SET SELECTION  : RANDOM                          
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.191                           
REMARK   3   R VALUE            (WORKING SET) : 0.190                           
REMARK   3   FREE R VALUE                     : 0.220                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.000                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 1265                            
REMARK   3                                                                      
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.                                  
REMARK   3   TOTAL NUMBER OF BINS USED           : 20                           
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : 1.75                         
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : 1.79                         
REMARK   3   REFLECTION IN BIN     (WORKING SET) : 1776                         
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : 99.10                        
REMARK   3   BIN R VALUE           (WORKING SET) : 0.2260                       
REMARK   3   BIN FREE R VALUE SET COUNT          : 94                           
REMARK   3   BIN FREE R VALUE                    : 0.2540                       
REMARK   3                                                                      
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.                    
REMARK   3   PROTEIN ATOMS            : 1528                                    
REMARK   3   NUCLEIC ACID ATOMS       : 0                                       
REMARK   3   HETEROGEN ATOMS          : 33                                      
REMARK   3   SOLVENT ATOMS            : 209                                     
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : 34.27                          
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 26.93                          
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : -0.63000                                             
REMARK   3    B22 (A**2) : -1.44000                                             
REMARK   3    B33 (A**2) : 1.89000                                              
REMARK   3    B12 (A**2) : 0.00000                                              
REMARK   3    B13 (A**2) : -0.70000                                             
REMARK   3    B23 (A**2) : -0.00000                                             
REMARK   3                                                                      
REMARK   3  ESTIMATED OVERALL COORDINATE ERROR.                                 
REMARK   3   ESU BASED ON R VALUE                            (A): 0.160         
REMARK   3   ESU BASED ON FREE R VALUE                       (A): 0.107         
REMARK   3   ESU BASED ON MAXIMUM LIKELIHOOD                 (A): NULL          
REMARK   3   ESU FOR B VALUES BASED ON MAXIMUM LIKELIHOOD (A**2): NULL          
REMARK   3                                                                      
REMARK   3 CORRELATION COEFFICIENTS.                                            
REMARK   3   CORRELATION COEFFICIENT FO-FC      : 0.963                         
REMARK   3   CORRELATION COEFFICIENT FO-FC FREE : 0.958                         
REMARK   3                                                                      
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES        COUNT    RMS    WEIGHT      
REMARK   3   BOND LENGTHS REFINED ATOMS        (A):  1600 ; 0.013 ; 0.020       
REMARK   3   BOND LENGTHS OTHERS               (A):  NULL ;  NULL ;  NULL       
REMARK   3   BOND ANGLES REFINED ATOMS   (DEGREES):  2167 ; 1.214 ; 1.979       
REMARK   3   BOND ANGLES OTHERS          (DEGREES):  NULL ;  NULL ;  NULL       
REMARK   3   TORSION ANGLES, PERIOD 1    (DEGREES):   195 ; 5.144 ; 5.000       
REMARK   3   TORSION ANGLES, PERIOD 2    (DEGREES):    66 ;33.297 ;24.394       
REMARK   3   TORSION ANGLES, PERIOD 3    (DEGREES):   275 ;11.902 ;15.000       
REMARK   3   TORSION ANGLES, PERIOD 4    (DEGREES):     7 ;22.178 ;15.000       
REMARK   3   CHIRAL-CENTER RESTRAINTS       (A**3):   244 ; 0.075 ; 0.200       
REMARK   3   GENERAL PLANES REFINED ATOMS      (A):  1169 ; 0.006 ; 0.021       
REMARK   3   GENERAL PLANES OTHERS             (A):  NULL ;  NULL ;  NULL       
REMARK   3   NON-BONDED CONTACTS REFINED ATOMS (A):  NULL ;  NULL ;  NULL       
REMARK   3   NON-BONDED CONTACTS OTHERS        (A):  NULL ;  NULL ;  NULL       
REMARK   3   NON-BONDED TORSION REFINED ATOMS  (A):  NULL ;  NULL ;  NULL       
REMARK   3   NON-BONDED TORSION OTHERS         (A):  NULL ;  NULL ;  NULL       
REMARK   3   H-BOND (X...Y) REFINED ATOMS      (A):  NULL ;  NULL ;  NULL       
REMARK   3   H-BOND (X...Y) OTHERS             (A):  NULL ;  NULL ;  NULL       
REMARK   3   POTENTIAL METAL-ION REFINED ATOMS (A):  NULL ;  NULL ;  NULL       
REMARK   3   POTENTIAL METAL-ION OTHERS        (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY VDW REFINED ATOMS        (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY VDW OTHERS               (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY H-BOND REFINED ATOMS     (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY H-BOND OTHERS            (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY METAL-ION REFINED ATOMS  (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY METAL-ION OTHERS         (A):  NULL ;  NULL ;  NULL       
REMARK   3                                                                      
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.     COUNT   RMS    WEIGHT      
REMARK   3   MAIN-CHAIN BOND REFINED ATOMS  (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   MAIN-CHAIN BOND OTHER ATOMS    (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   MAIN-CHAIN ANGLE REFINED ATOMS (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   SIDE-CHAIN BOND REFINED ATOMS  (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   SIDE-CHAIN ANGLE REFINED ATOMS (A**2):  NULL ;  NULL ;  NULL       
REMARK   3                                                                      
REMARK   3 ANISOTROPIC THERMAL FACTOR RESTRAINTS.    COUNT   RMS   WEIGHT       
REMARK   3   RIGID-BOND RESTRAINTS          (A**2):  1600 ; 4.245 ; 3.000       
REMARK   3   SPHERICITY; FREE ATOMS         (A**2):   209 ; 0.000 ; 5.000       
REMARK   3   SPHERICITY; BONDED ATOMS       (A**2):  1569 ; 0.000 ; 5.000       
REMARK   3                                                                      
REMARK   3  NCS RESTRAINTS STATISTICS                                           
REMARK   3   NUMBER OF DIFFERENT NCS GROUPS : NULL                              
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : NULL                                       
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED : BABINET MODEL WITH MASK                              
REMARK   3   PARAMETERS FOR MASK CALCULATION                                    
REMARK   3   VDW PROBE RADIUS   : 1.20                                          
REMARK   3   ION PROBE RADIUS   : 0.80                                          
REMARK   3   SHRINKAGE RADIUS   : 0.80                                          
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: HYDROGENS HAVE BEEN USED IF PRESENT IN    
REMARK   3  THE INPUT U VALUES : REFINED INDIVIDUALLY                           
REMARK   4                                                                      
REMARK   4 4ERV COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 29-APR-12.                  
REMARK 100 THE RCSB ID CODE IS RCSB071991.                                      
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 29-NOV-11                          
REMARK 200  TEMPERATURE           (KELVIN) : 100                                
REMARK 200  PH                             : 9.5                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : CLSI                               
REMARK 200  BEAMLINE                       : 08ID-1                             
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.9795                             
REMARK 200  MONOCHROMATOR                  : DOUBLE CRYSTAL MONOCHROMATOR       
REMARK 200                                   WITH CRYO-COOLED 1ST CRYSTAL       
REMARK 200                                   SAGITTALLY BENT 2ND CRYSTAL        
REMARK 200                                   FOLLOWED BY VERTICALLY FOCUSING    
REMARK 200                                   MIRROR                             
REMARK 200  OPTICS                         : DOUBLE CRYSTAL MONOCHROMATOR,      
REMARK 200                                   MIRRORS                            
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : MARMOSAIC 225 MM CCD               
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS                                
REMARK 200  DATA SCALING SOFTWARE          : XDS                                
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 25284                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.750                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 50.000                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : -3.000                             
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 98.1                               
REMARK 200  DATA REDUNDANCY                : 3.600                              
REMARK 200  R MERGE                    (I) : 0.03500                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 20.3000                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.75                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 1.80                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 99.2                               
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL                               
REMARK 200  R MERGE FOR SHELL          (I) : 0.45200                            
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 3.150                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: PHASER                                                
REMARK 200 STARTING MODEL: NULL                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 54.71                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.72                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 1.4M AMMONIUM SULFATE, 0.1M BICINE, PH   
REMARK 280  9.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 295K                
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: C 1 2 1                          
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,Y,-Z                                                 
REMARK 290       3555   X+1/2,Y+1/2,Z                                           
REMARK 290       4555   -X+1/2,Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   3  1.000000  0.000000  0.000000       46.78500            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       29.27000            
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   4 -1.000000  0.000000  0.000000       46.78500            
REMARK 290   SMTRY2   4  0.000000  1.000000  0.000000       29.27000            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1, 2                                                    
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A                                     
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 2                                                       
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC                    
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 5970 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 19860 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -111.0 KCAL/MOL                       
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A                                     
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350   BIOMT1   2 -1.000000  0.000000  0.000000       93.57000            
REMARK 350   BIOMT2   2  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   2  0.000000  0.000000 -1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     SER A  2594                                                      
REMARK 465     ASN A  2595                                                      
REMARK 465     ALA A  2596                                                      
REMARK 465     ASN A  2597                                                      
REMARK 465     GLU A  2691                                                      
REMARK 465     SER A  2708                                                      
REMARK 465     GLN A  2709                                                      
REMARK 465     ALA A  2710                                                      
REMARK 465     ASN A  2711                                                      
REMARK 465     GLN A  2712                                                      
REMARK 465     GLY A  2713                                                      
REMARK 465     ASN A  2714                                                      
REMARK 465     SER A  2715                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     GLU A2612    CG   CD   OE1  OE2                                  
REMARK 470     LYS A2613    CG   CD   CE   NZ                                   
REMARK 470     GLN A2635    CG   CD   OE1  NE2                                  
REMARK 470     ASN A2647    CG   OD1  ND2                                       
REMARK 470     LYS A2658    CG   CD   CE   NZ                                   
REMARK 470     GLU A2687    CG   CD   OE1  OE2                                  
REMARK 470     ARG A2688    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     LYS A2690    CG   CD   CE   NZ                                   
REMARK 470     GLU A2693    CG   CD   OE1  OE2                                  
REMARK 470     GLU A2700    CG   CD   OE1  OE2                                  
REMARK 470     LYS A2703    CG   CD   CE   NZ                                   
REMARK 470     VAL A2707    CG1  CG2                                            
REMARK 470     ARG A2730    CG   CD   NE   CZ   NH1  NH2                        
REMARK 800                                                                      
REMARK 800 SITE                                                                 
REMARK 800 SITE_IDENTIFIER: AC1                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE SO4 A 2901                
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC2                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE SO4 A 2902                
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC3                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE SO4 A 2903                
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC4                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE GOL A 2904                
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC5                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE GOL A 2905                
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC6                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE GOL A 2906                
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 4ERT   RELATED DB: PDB                                   
REMARK 900 RYR1 2734-2940                                                       
REMARK 900 RELATED ID: 4ETT   RELATED DB: PDB                                   
REMARK 900 RYR1 E2764K                                                          
REMARK 900 RELATED ID: 4ESU   RELATED DB: PDB                                   
REMARK 900 RYR1 S2776M                                                          
REMARK 900 RELATED ID: 4ETU   RELATED DB: PDB                                   
REMARK 900 RYR1 R2939S                                                          
REMARK 900 RELATED ID: 4ETV   RELATED DB: PDB                                   
REMARK 900 RYR2 K2879A                                                          
DBREF  4ERV A 2597  2800  UNP    Q15413   RYR3_HUMAN    2597   2800             
SEQADV 4ERV SER A 2594  UNP  Q15413              EXPRESSION TAG                 
SEQADV 4ERV ASN A 2595  UNP  Q15413              EXPRESSION TAG                 
SEQADV 4ERV ALA A 2596  UNP  Q15413              EXPRESSION TAG                 
SEQADV 4ERV ALA A 2775  UNP  Q15413    LYS  2775 ENGINEERED MUTATION            
SEQRES   1 A  207  SER ASN ALA ASN PHE ASP PRO LYS PRO ILE ASN THR MET          
SEQRES   2 A  207  ASN PHE SER LEU PRO GLU LYS LEU GLU TYR ILE VAL THR          
SEQRES   3 A  207  LYS TYR ALA GLU HIS SER HIS ASP LYS TRP ALA CYS ASP          
SEQRES   4 A  207  LYS SER GLN SER GLY TRP LYS TYR GLY ILE SER LEU ASP          
SEQRES   5 A  207  GLU ASN VAL LYS THR HIS PRO LEU ILE ARG PRO PHE LYS          
SEQRES   6 A  207  THR LEU THR GLU LYS GLU LYS GLU ILE TYR ARG TRP PRO          
SEQRES   7 A  207  ALA ARG GLU SER LEU LYS THR MET LEU ALA VAL GLY TRP          
SEQRES   8 A  207  THR VAL GLU ARG THR LYS GLU GLY GLU ALA LEU VAL GLN          
SEQRES   9 A  207  GLN ARG GLU ASN GLU LYS LEU ARG SER VAL SER GLN ALA          
SEQRES  10 A  207  ASN GLN GLY ASN SER TYR SER PRO ALA PRO LEU ASP LEU          
SEQRES  11 A  207  SER ASN VAL VAL LEU SER ARG GLU LEU GLN GLY MET VAL          
SEQRES  12 A  207  GLU VAL VAL ALA GLU ASN TYR HIS ASN ILE TRP ALA LYS          
SEQRES  13 A  207  LYS LYS LYS LEU GLU LEU GLU SER LYS GLY GLY GLY SER          
SEQRES  14 A  207  HIS PRO LEU LEU VAL PRO TYR ASP THR LEU THR ALA ALA          
SEQRES  15 A  207  GLU LYS PHE LYS ASP ARG GLU LYS ALA GLN ASP LEU PHE          
SEQRES  16 A  207  LYS PHE LEU GLN VAL ASN GLY ILE ILE VAL SER ARG              
HET    SO4  A2901       5                                                       
HET    SO4  A2902       5                                                       
HET    SO4  A2903       5                                                       
HET    GOL  A2904       6                                                       
HET    GOL  A2905       6                                                       
HET    GOL  A2906       6                                                       
HETNAM     SO4 SULFATE ION                                                      
HETNAM     GOL GLYCEROL                                                         
HETSYN     GOL GLYCERIN; PROPANE-1,2,3-TRIOL                                    
FORMUL   2  SO4    3(O4 S 2-)                                                   
FORMUL   5  GOL    3(C3 H8 O3)                                                  
FORMUL   8  HOH   *209(H2 O)                                                    
HELIX    1   1 PRO A 2611  SER A 2636  1                                  26    
HELIX    2   2 PRO A 2656  LEU A 2660  5                                   5    
HELIX    3   3 THR A 2661  VAL A 2682  1                                  22    
HELIX    4   4 ALA A 2694  ARG A 2705  1                                  12    
HELIX    5   5 ASP A 2722  VAL A 2726  5                                   5    
HELIX    6   6 SER A 2729  LYS A 2758  1                                  30    
HELIX    7   7 PRO A 2768  LEU A 2772  5                                   5    
HELIX    8   8 THR A 2773  VAL A 2793  1                                  21    
SHEET    1   A 2 THR A2685  ARG A2688  0                                        
SHEET    2   A 2 ILE A2796  SER A2799 -1  O  ILE A2797   N  GLU A2687           
SITE     1 AC1  8 TRP A2747  LYS A2750  LYS A2751  GOL A2906                    
SITE     2 AC1  8 HOH A3050  HOH A3100  HOH A3133  HOH A3151                    
SITE     1 AC2  3 ALA A2774  LYS A2777  ARG A2781                               
SITE     1 AC3  6 TRP A2629  LYS A2633  LYS A2779  GOL A2904                    
SITE     2 AC3  6 HOH A3092  HOH A3206                                          
SITE     1 AC4  9 LYS A2633  TRP A2638  HIS A2651  ALA A2775                    
SITE     2 AC4  9 PHE A2778  SO4 A2903  HOH A3092  HOH A3168                    
SITE     3 AC4  9 HOH A3206                                                     
SITE     1 AC5  7 LYS A2663  PRO A2764  VAL A2767  THR A2771                    
SITE     2 AC5  7 GLU A2776  HOH A3016  HOH A3201                               
SITE     1 AC6  5 TRP A2747  SO4 A2901  HOH A3087  HOH A3205                    
SITE     2 AC6  5 HOH A3208                                                     
CRYST1   93.570   58.540   47.460  90.00  97.21  90.00 C 1 2 1       4          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.010687  0.000000  0.001352        0.00000                         
SCALE2      0.000000  0.017082  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.021238        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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