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Database: PDB
Entry: 5EOA
LinkDB: 5EOA
Original site: 5EOA 
HEADER    PROTEIN BINDING/TRANSFERASE             10-NOV-15   5EOA              
TITLE     CRYSTAL STRUCTURE OF OPTN E50K MUTANT AND TBK1 COMPLEX                
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: OPTINEURIN;                                                
COMPND   3 CHAIN: A, B;                                                         
COMPND   4 FRAGMENT: UNP RESIDUES 26-103;                                       
COMPND   5 SYNONYM: E3-14.7K-INTERACTING PROTEIN,FIP-2,HUNTINGTIN YEAST PARTNER 
COMPND   6 L,HUNTINGTIN-INTERACTING PROTEIN 7,HIP-7,HUNTINGTIN-INTERACTING      
COMPND   7 PROTEIN L,NEMO-RELATED PROTEIN,OPTIC NEUROPATHY-INDUCING PROTEIN,    
COMPND   8 TRANSCRIPTION FACTOR IIIA-INTERACTING PROTEIN,TFIIIA-INTP;           
COMPND   9 ENGINEERED: YES;                                                     
COMPND  10 MUTATION: YES;                                                       
COMPND  11 MOL_ID: 2;                                                           
COMPND  12 MOLECULE: SERINE/THREONINE-PROTEIN KINASE TBK1;                      
COMPND  13 CHAIN: C, D;                                                         
COMPND  14 FRAGMENT: UNP RESIDUES 677-729;                                      
COMPND  15 SYNONYM: NF-KAPPA-B-ACTIVATING KINASE,T2K,TANK-BINDING KINASE 1;     
COMPND  16 EC: 2.7.11.1;                                                        
COMPND  17 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: OPTN, FIP2, GLC1E, HIP7, HYPL, NRP;                            
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE   8 MOL_ID: 2;                                                           
SOURCE   9 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  10 ORGANISM_COMMON: HUMAN;                                              
SOURCE  11 ORGANISM_TAXID: 9606;                                                
SOURCE  12 GENE: TBK1, NAK;                                                     
SOURCE  13 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  14 EXPRESSION_SYSTEM_TAXID: 562                                         
KEYWDS    OPTINEURIN, TBK1, POAG, ALS, PROTEIN BINDING-TRANSFERASE COMPLEX      
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    F.LI,X.XIE,J.LIU,L.PAN                                                
REVDAT   1   28-SEP-16 5EOA    0                                                
JRNL        AUTH   F.LI,X.XIE,Y.WANG,J.LIU,X.CHENG,Y.GUO,Y.GONG,S.HU,L.PAN      
JRNL        TITL   STRUCTURAL INSIGHTS INTO THE INTERACTION AND DISEASE         
JRNL        TITL 2 MECHANISM OF NEURODEGENERATIVE DISEASE-ASSOCIATED OPTINEURIN 
JRNL        TITL 3 AND TBK1 PROTEINS.                                           
JRNL        REF    NAT COMMUN                    V.   7 12708 2016              
JRNL        REFN                   ESSN 2041-1723                               
JRNL        PMID   27620379                                                     
JRNL        DOI    10.1038/NCOMMS12708                                          
REMARK   2                                                                      
REMARK   2 RESOLUTION.    2.50 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX 1.8.2_1309                                    
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : ML                                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.50                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 44.44                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.350                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.6                           
REMARK   3   NUMBER OF REFLECTIONS             : 11206                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.202                           
REMARK   3   R VALUE            (WORKING SET) : 0.199                           
REMARK   3   FREE R VALUE                     : 0.267                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 4.770                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 535                             
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 44.4509 -  3.9727    0.99     2795   129  0.1761 0.2213        
REMARK   3     2  3.9727 -  3.1535    1.00     2670   124  0.1989 0.2862        
REMARK   3     3  3.1535 -  2.7550    1.00     2634   145  0.2344 0.2849        
REMARK   3     4  2.7550 -  2.5031    0.99     2572   137  0.2599 0.3753        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL                       
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : NULL                                          
REMARK   3   B_SOL              : NULL                                          
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.380            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 26.720           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL                           
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.007           1913                                  
REMARK   3   ANGLE     :  0.896           2549                                  
REMARK   3   CHIRALITY :  0.057            281                                  
REMARK   3   PLANARITY :  0.003            327                                  
REMARK   3   DIHEDRAL  : 18.473            778                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : 6                                          
REMARK   3   TLS GROUP : 1                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 30 THROUGH 36 )                   
REMARK   3    ORIGIN FOR THE GROUP (A):   1.9548 -19.9356 -59.6639              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.8781 T22:   0.5037                                     
REMARK   3      T33:   0.5587 T12:  -0.0155                                     
REMARK   3      T13:  -0.0854 T23:  -0.0977                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   3.7403 L22:   7.2173                                     
REMARK   3      L33:   5.8576 L12:  -2.3807                                     
REMARK   3      L13:  -1.0167 L23:   2.7479                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.6270 S12:   0.9608 S13:   0.0489                       
REMARK   3      S21:   0.2175 S22:  -0.2173 S23:  -0.2465                       
REMARK   3      S31:  -1.7425 S32:   0.3722 S33:  -0.5450                       
REMARK   3   TLS GROUP : 2                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 37 THROUGH 101 )                  
REMARK   3    ORIGIN FOR THE GROUP (A):  -2.0185  -6.9512 -12.1683              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3090 T22:   0.4251                                     
REMARK   3      T33:   0.4960 T12:  -0.0185                                     
REMARK   3      T13:  -0.0021 T23:   0.0601                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.3912 L22:   1.0881                                     
REMARK   3      L33:   8.2260 L12:  -0.3237                                     
REMARK   3      L13:   1.2268 L23:  -1.6762                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.1661 S12:  -0.1348 S13:  -0.0048                       
REMARK   3      S21:   0.1151 S22:   0.0930 S23:   0.0222                       
REMARK   3      S31:  -0.7642 S32:  -0.6570 S33:   0.1360                       
REMARK   3   TLS GROUP : 3                                                      
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 30 THROUGH 36 )                   
REMARK   3    ORIGIN FOR THE GROUP (A):  -6.6828   4.3607 -59.0279              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.7782 T22:   0.5767                                     
REMARK   3      T33:   0.6621 T12:  -0.1187                                     
REMARK   3      T13:   0.0212 T23:  -0.0256                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   4.7894 L22:   4.6703                                     
REMARK   3      L33:   5.6205 L12:  -4.7200                                     
REMARK   3      L13:   2.7264 L23:  -2.4107                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.7072 S12:   0.0521 S13:  -0.3069                       
REMARK   3      S21:  -0.5205 S22:  -0.0651 S23:   0.4323                       
REMARK   3      S31:   1.2835 S32:  -1.0687 S33:  -0.6067                       
REMARK   3   TLS GROUP : 4                                                      
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 37 THROUGH 102 )                  
REMARK   3    ORIGIN FOR THE GROUP (A):   6.1827  -8.7288 -14.0029              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3404 T22:   0.4853                                     
REMARK   3      T33:   0.5166 T12:  -0.0343                                     
REMARK   3      T13:  -0.0331 T23:   0.0562                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.3637 L22:   0.6200                                     
REMARK   3      L33:   2.5803 L12:  -0.3169                                     
REMARK   3      L13:   0.6679 L23:  -0.1854                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.2877 S12:  -0.0257 S13:  -0.0316                       
REMARK   3      S21:  -0.0404 S22:  -0.2378 S23:  -0.1151                       
REMARK   3      S31:  -0.2751 S32:   0.5482 S33:   0.5372                       
REMARK   3   TLS GROUP : 5                                                      
REMARK   3    SELECTION: CHAIN 'C' AND (RESID 678 THROUGH 720 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):   2.3573 -13.7001 -36.1225              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.4274 T22:   0.4118                                     
REMARK   3      T33:   0.4735 T12:   0.0095                                     
REMARK   3      T13:   0.0443 T23:  -0.0519                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.2244 L22:   2.3346                                     
REMARK   3      L33:   2.3538 L12:  -0.0004                                     
REMARK   3      L13:  -0.7322 L23:  -0.5407                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0117 S12:  -0.0587 S13:  -0.0016                       
REMARK   3      S21:  -0.3479 S22:   0.0967 S23:  -0.2359                       
REMARK   3      S31:   1.0197 S32:   0.4529 S33:  -0.0321                       
REMARK   3   TLS GROUP : 6                                                      
REMARK   3    SELECTION: CHAIN 'D' AND (RESID 679 THROUGH 719 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  -2.0181  -1.8388 -35.5943              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.4707 T22:   0.4039                                     
REMARK   3      T33:   0.5261 T12:  -0.0591                                     
REMARK   3      T13:  -0.0195 T23:   0.1119                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   1.1694 L22:   1.2282                                     
REMARK   3      L33:   9.4848 L12:  -0.5429                                     
REMARK   3      L13:   3.3315 L23:  -1.2861                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.4126 S12:  -0.0306 S13:   0.1163                       
REMARK   3      S21:   0.0068 S22:   0.1680 S23:   0.0907                       
REMARK   3      S31:  -1.5773 S32:  -0.0975 S33:   0.1735                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 5EOA COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 12-NOV-15.                  
REMARK 100 THE DEPOSITION ID IS D_1000215250.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 25-JUL-15                          
REMARK 200  TEMPERATURE           (KELVIN) : 291.15                             
REMARK 200  PH                             : 6.0                                
REMARK 200  NUMBER OF CRYSTALS USED        : NULL                               
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : SSRF                               
REMARK 200  BEAMLINE                       : BL17U                              
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.9791                             
REMARK 200  MONOCHROMATOR                  : NULL                               
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : ADSC QUANTUM 315                   
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : HKL-2000                           
REMARK 200  DATA SCALING SOFTWARE          : HKL-2000                           
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 11226                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.500                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 50.000                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.7                               
REMARK 200  DATA REDUNDANCY                : 3.500                              
REMARK 200  R MERGE                    (I) : NULL                               
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 10.4200                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : NULL                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : NULL                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : NULL                               
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL                               
REMARK 200  R MERGE FOR SHELL          (I) : NULL                               
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : NULL                               
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: NULL                         
REMARK 200 SOFTWARE USED: PHASER                                                
REMARK 200 STARTING MODEL: NULL                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 49.20                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.42                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.1M MES MONOHYDRATE PH 6.0, 14% W/V     
REMARK 280  POLYETHYLENE GLYCOL 4000, EVAPORATION, TEMPERATURE 291.5K           
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 21 21                       
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X+1/2,-Y,Z+1/2                                         
REMARK 290       3555   -X,Y+1/2,-Z+1/2                                         
REMARK 290       4555   X+1/2,-Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000       18.41200            
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       76.65250            
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       27.27450            
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000       76.65250            
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000       18.41200            
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000       27.27450            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TETRAMERIC                        
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: TETRAMERIC                 
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 9740 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 13740 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -95.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, C, D                            
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     GLY A    22                                                      
REMARK 465     PRO A    23                                                      
REMARK 465     GLY A    24                                                      
REMARK 465     SER A    25                                                      
REMARK 465     HIS A    26                                                      
REMARK 465     LEU A    27                                                      
REMARK 465     ALA A    28                                                      
REMARK 465     HIS A    29                                                      
REMARK 465     HIS A   102                                                      
REMARK 465     GLU A   103                                                      
REMARK 465     GLY B    22                                                      
REMARK 465     PRO B    23                                                      
REMARK 465     GLY B    24                                                      
REMARK 465     SER B    25                                                      
REMARK 465     HIS B    26                                                      
REMARK 465     LEU B    27                                                      
REMARK 465     ALA B    28                                                      
REMARK 465     HIS B    29                                                      
REMARK 465     GLU B   103                                                      
REMARK 465     GLY C   673                                                      
REMARK 465     PRO C   674                                                      
REMARK 465     GLY C   675                                                      
REMARK 465     SER C   676                                                      
REMARK 465     TYR C   677                                                      
REMARK 465     GLY C   721                                                      
REMARK 465     GLY C   722                                                      
REMARK 465     LEU C   723                                                      
REMARK 465     ARG C   724                                                      
REMARK 465     ASN C   725                                                      
REMARK 465     VAL C   726                                                      
REMARK 465     ASP C   727                                                      
REMARK 465     CYS C   728                                                      
REMARK 465     LEU C   729                                                      
REMARK 465     GLY D   673                                                      
REMARK 465     PRO D   674                                                      
REMARK 465     GLY D   675                                                      
REMARK 465     SER D   676                                                      
REMARK 465     TYR D   677                                                      
REMARK 465     PRO D   678                                                      
REMARK 465     ASP D   720                                                      
REMARK 465     GLY D   721                                                      
REMARK 465     GLY D   722                                                      
REMARK 465     LEU D   723                                                      
REMARK 465     ARG D   724                                                      
REMARK 465     ASN D   725                                                      
REMARK 465     VAL D   726                                                      
REMARK 465     ASP D   727                                                      
REMARK 465     CYS D   728                                                      
REMARK 465     LEU D   729                                                      
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    ASN D 681      -20.32     65.68                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 5EOF   RELATED DB: PDB                                   
DBREF  5EOA A   26   103  UNP    Q96CV9   OPTN_HUMAN      26    103             
DBREF  5EOA B   26   103  UNP    Q96CV9   OPTN_HUMAN      26    103             
DBREF  5EOA C  677   729  UNP    Q9UHD2   TBK1_HUMAN     677    729             
DBREF  5EOA D  677   729  UNP    Q9UHD2   TBK1_HUMAN     677    729             
SEQADV 5EOA GLY A   22  UNP  Q96CV9              EXPRESSION TAG                 
SEQADV 5EOA PRO A   23  UNP  Q96CV9              EXPRESSION TAG                 
SEQADV 5EOA GLY A   24  UNP  Q96CV9              EXPRESSION TAG                 
SEQADV 5EOA SER A   25  UNP  Q96CV9              EXPRESSION TAG                 
SEQADV 5EOA LYS A   50  UNP  Q96CV9    GLU    50 ENGINEERED MUTATION            
SEQADV 5EOA GLY B   22  UNP  Q96CV9              EXPRESSION TAG                 
SEQADV 5EOA PRO B   23  UNP  Q96CV9              EXPRESSION TAG                 
SEQADV 5EOA GLY B   24  UNP  Q96CV9              EXPRESSION TAG                 
SEQADV 5EOA SER B   25  UNP  Q96CV9              EXPRESSION TAG                 
SEQADV 5EOA LYS B   50  UNP  Q96CV9    GLU    50 ENGINEERED MUTATION            
SEQADV 5EOA GLY C  673  UNP  Q9UHD2              EXPRESSION TAG                 
SEQADV 5EOA PRO C  674  UNP  Q9UHD2              EXPRESSION TAG                 
SEQADV 5EOA GLY C  675  UNP  Q9UHD2              EXPRESSION TAG                 
SEQADV 5EOA SER C  676  UNP  Q9UHD2              EXPRESSION TAG                 
SEQADV 5EOA GLY D  673  UNP  Q9UHD2              EXPRESSION TAG                 
SEQADV 5EOA PRO D  674  UNP  Q9UHD2              EXPRESSION TAG                 
SEQADV 5EOA GLY D  675  UNP  Q9UHD2              EXPRESSION TAG                 
SEQADV 5EOA SER D  676  UNP  Q9UHD2              EXPRESSION TAG                 
SEQRES   1 A   82  GLY PRO GLY SER HIS LEU ALA HIS PRO ASN LEU ASP THR          
SEQRES   2 A   82  PHE THR PRO GLU GLU LEU LEU GLN GLN MET LYS GLU LEU          
SEQRES   3 A   82  LEU THR LYS ASN HIS GLN LEU LYS GLU ALA MET LYS LEU          
SEQRES   4 A   82  ASN ASN GLN ALA MET LYS GLY ARG PHE GLU GLU LEU SER          
SEQRES   5 A   82  ALA TRP THR GLU LYS GLN LYS GLU GLU ARG GLN PHE PHE          
SEQRES   6 A   82  GLU ILE GLN SER LYS GLU ALA LYS GLU ARG LEU MET ALA          
SEQRES   7 A   82  LEU SER HIS GLU                                              
SEQRES   1 B   82  GLY PRO GLY SER HIS LEU ALA HIS PRO ASN LEU ASP THR          
SEQRES   2 B   82  PHE THR PRO GLU GLU LEU LEU GLN GLN MET LYS GLU LEU          
SEQRES   3 B   82  LEU THR LYS ASN HIS GLN LEU LYS GLU ALA MET LYS LEU          
SEQRES   4 B   82  ASN ASN GLN ALA MET LYS GLY ARG PHE GLU GLU LEU SER          
SEQRES   5 B   82  ALA TRP THR GLU LYS GLN LYS GLU GLU ARG GLN PHE PHE          
SEQRES   6 B   82  GLU ILE GLN SER LYS GLU ALA LYS GLU ARG LEU MET ALA          
SEQRES   7 B   82  LEU SER HIS GLU                                              
SEQRES   1 C   57  GLY PRO GLY SER TYR PRO SER SER ASN THR LEU VAL GLU          
SEQRES   2 C   57  MET THR LEU GLY MET LYS LYS LEU LYS GLU GLU MET GLU          
SEQRES   3 C   57  GLY VAL VAL LYS GLU LEU ALA GLU ASN ASN HIS ILE LEU          
SEQRES   4 C   57  GLU ARG PHE GLY SER LEU THR MET ASP GLY GLY LEU ARG          
SEQRES   5 C   57  ASN VAL ASP CYS LEU                                          
SEQRES   1 D   57  GLY PRO GLY SER TYR PRO SER SER ASN THR LEU VAL GLU          
SEQRES   2 D   57  MET THR LEU GLY MET LYS LYS LEU LYS GLU GLU MET GLU          
SEQRES   3 D   57  GLY VAL VAL LYS GLU LEU ALA GLU ASN ASN HIS ILE LEU          
SEQRES   4 D   57  GLU ARG PHE GLY SER LEU THR MET ASP GLY GLY LEU ARG          
SEQRES   5 D   57  ASN VAL ASP CYS LEU                                          
FORMUL   5  HOH   *24(H2 O)                                                     
HELIX    1 AA1 THR A   36  ALA A   99  1                                  64    
HELIX    2 AA2 THR B   36  HIS B  102  1                                  67    
HELIX    3 AA3 SER C  679  GLY C  715  1                                  37    
HELIX    4 AA4 THR D  682  THR D  718  1                                  37    
CISPEP   1 LEU A  100    SER A  101          0        -3.29                     
CRYST1   36.824   54.549  153.305  90.00  90.00  90.00 P 21 21 21    8          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.027156  0.000000  0.000000        0.00000                         
SCALE2      0.000000  0.018332  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.006523        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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