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HEADER    PROTEIN BINDING/TRANSFERASE             10-NOV-15   5EOF              
TITLE     CRYSTAL STRUCTURE OF OPTN NTD AND TBK1 CTD COMPLEX                    
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: OPTINEURIN;                                                
COMPND   3 CHAIN: A, B;                                                         
COMPND   4 FRAGMENT: UNP RESIDUES 26-103;                                       
COMPND   5 SYNONYM: E3-14.7K-INTERACTING PROTEIN,FIP-2,HUNTINGTIN YEAST PARTNER 
COMPND   6 L,HUNTINGTIN-INTERACTING PROTEIN 7,HIP-7,HUNTINGTIN-INTERACTING      
COMPND   7 PROTEIN L,NEMO-RELATED PROTEIN,OPTIC NEUROPATHY-INDUCING PROTEIN,    
COMPND   8 TRANSCRIPTION FACTOR IIIA-INTERACTING PROTEIN,TFIIIA-INTP;           
COMPND   9 ENGINEERED: YES;                                                     
COMPND  10 MOL_ID: 2;                                                           
COMPND  11 MOLECULE: SERINE/THREONINE-PROTEIN KINASE TBK1;                      
COMPND  12 CHAIN: C, D;                                                         
COMPND  13 FRAGMENT: UNP RESIDUES 677-729;                                      
COMPND  14 SYNONYM: NF-KAPPA-B-ACTIVATING KINASE,T2K,TANK-BINDING KINASE 1;     
COMPND  15 EC: 2.7.11.1;                                                        
COMPND  16 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: OPTN, FIP2, GLC1E, HIP7, HYPL, NRP;                            
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE   8 MOL_ID: 2;                                                           
SOURCE   9 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE  10 ORGANISM_COMMON: HUMAN;                                              
SOURCE  11 ORGANISM_TAXID: 9606;                                                
SOURCE  12 GENE: TBK1, NAK;                                                     
SOURCE  13 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE  14 EXPRESSION_SYSTEM_TAXID: 562                                         
KEYWDS    OPTN, TBK1, ALS, AUTOPHAGY, PROTEIN BINDING-TRANSFERASE COMPLEX       
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    F.LI,X.XIE,J.LIU,L.PAN                                                
REVDAT   1   28-SEP-16 5EOF    0                                                
JRNL        AUTH   F.LI,X.XIE,Y.WANG,J.LIU,X.CHENG,Y.GUO,Y.GONG,S.HU,L.PAN      
JRNL        TITL   STRUCTURAL INSIGHTS INTO THE INTERACTION AND DISEASE         
JRNL        TITL 2 MECHANISM OF NEURODEGENERATIVE DISEASE-ASSOCIATED OPTINEURIN 
JRNL        TITL 3 AND TBK1 PROTEINS.                                           
JRNL        REF    NAT COMMUN                    V.   7 12708 2016              
JRNL        REFN                   ESSN 2041-1723                               
JRNL        PMID   27620379                                                     
JRNL        DOI    10.1038/NCOMMS12708                                          
REMARK   2                                                                      
REMARK   2 RESOLUTION.    2.05 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : PHENIX 1.8.2_1309                                    
REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : ML                                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.05                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 37.34                          
REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.360                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 99.8                           
REMARK   3   NUMBER OF REFLECTIONS             : 19973                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.209                           
REMARK   3   R VALUE            (WORKING SET) : 0.208                           
REMARK   3   FREE R VALUE                     : 0.245                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.110                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 1020                            
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
REMARK   3     1 37.3503 -  3.9183    0.99     2855   166  0.1879 0.1929        
REMARK   3     2  3.9183 -  3.1106    1.00     2747   143  0.1978 0.2526        
REMARK   3     3  3.1106 -  2.7175    1.00     2713   131  0.2346 0.3065        
REMARK   3     4  2.7175 -  2.4691    1.00     2676   143  0.2242 0.2825        
REMARK   3     5  2.4691 -  2.2921    1.00     2640   156  0.2298 0.2904        
REMARK   3     6  2.2921 -  2.1570    1.00     2666   139  0.2330 0.3423        
REMARK   3     7  2.1570 -  2.0490    1.00     2656   142  0.2528 0.2851        
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL                       
REMARK   3   SOLVENT RADIUS     : 1.11                                          
REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
REMARK   3   K_SOL              : NULL                                          
REMARK   3   B_SOL              : NULL                                          
REMARK   3                                                                      
REMARK   3  ERROR ESTIMATES.                                                    
REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.260            
REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 27.820           
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL                           
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  TWINNING INFORMATION.                                               
REMARK   3   FRACTION: NULL                                                     
REMARK   3   OPERATOR: NULL                                                     
REMARK   3                                                                      
REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
REMARK   3                 RMSD          COUNT                                  
REMARK   3   BOND      :  0.007           1949                                  
REMARK   3   ANGLE     :  0.960           2610                                  
REMARK   3   CHIRALITY :  0.062            289                                  
REMARK   3   PLANARITY :  0.004            338                                  
REMARK   3   DIHEDRAL  : 16.657            788                                  
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : 6                                          
REMARK   3   TLS GROUP : 1                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 29 THROUGH 36 )                   
REMARK   3    ORIGIN FOR THE GROUP (A):  -3.8406  20.7277 -59.8366              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   1.0148 T22:  -0.0209                                     
REMARK   3      T33:   0.4280 T12:  -0.1242                                     
REMARK   3      T13:  -0.0245 T23:   0.1605                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   2.1777 L22:   8.4171                                     
REMARK   3      L33:   0.1052 L12:  -3.4854                                     
REMARK   3      L13:  -0.0871 L23:   0.6642                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.1992 S12:   0.0891 S13:  -0.0528                       
REMARK   3      S21:  -0.4646 S22:  -0.0069 S23:   0.9072                       
REMARK   3      S31:   0.0236 S32:   0.0419 S33:  -0.1843                       
REMARK   3   TLS GROUP : 2                                                      
REMARK   3    SELECTION: CHAIN 'A' AND (RESID 37 THROUGH 102 )                  
REMARK   3    ORIGIN FOR THE GROUP (A):   2.3353   6.9235 -11.7019              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2214 T22:   0.2034                                     
REMARK   3      T33:   0.2785 T12:  -0.0101                                     
REMARK   3      T13:   0.0370 T23:  -0.0220                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.4398 L22:   0.7493                                     
REMARK   3      L33:   6.7113 L12:  -0.3077                                     
REMARK   3      L13:  -0.9703 L23:   1.8047                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.1756 S12:  -0.1027 S13:  -0.0452                       
REMARK   3      S21:   0.0667 S22:   0.0811 S23:   0.0635                       
REMARK   3      S31:   0.7184 S32:   0.2269 S33:   0.0678                       
REMARK   3   TLS GROUP : 3                                                      
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 29 THROUGH 36 )                   
REMARK   3    ORIGIN FOR THE GROUP (A):   5.3913  -4.4745 -60.5362              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.5658 T22:   0.4345                                     
REMARK   3      T33:   0.4987 T12:  -0.0742                                     
REMARK   3      T13:   0.1043 T23:  -0.0454                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   3.8877 L22:   5.3950                                     
REMARK   3      L33:   3.6030 L12:  -4.5491                                     
REMARK   3      L13:   0.1381 L23:  -0.5435                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.3512 S12:   0.5291 S13:   0.6336                       
REMARK   3      S21:  -0.1584 S22:  -0.3855 S23:  -1.0252                       
REMARK   3      S31:  -0.6441 S32:   0.9078 S33:   0.0900                       
REMARK   3   TLS GROUP : 4                                                      
REMARK   3    SELECTION: CHAIN 'B' AND (RESID 37 THROUGH 102 )                  
REMARK   3    ORIGIN FOR THE GROUP (A):  -5.8014   8.7930 -13.6432              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.2778 T22:   0.3046                                     
REMARK   3      T33:   0.2577 T12:  -0.0248                                     
REMARK   3      T13:   0.0235 T23:  -0.0511                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.1077 L22:   0.3434                                     
REMARK   3      L33:   2.4731 L12:  -0.3344                                     
REMARK   3      L13:  -0.6365 L23:   0.3103                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.0338 S12:  -0.0178 S13:   0.0147                       
REMARK   3      S21:  -0.1242 S22:   0.0407 S23:   0.0240                       
REMARK   3      S31:  -0.1613 S32:  -0.0225 S33:   0.0305                       
REMARK   3   TLS GROUP : 5                                                      
REMARK   3    SELECTION: CHAIN 'C' AND (RESID 678 THROUGH 720 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):  -2.4237  13.7250 -35.6328              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3787 T22:   0.2153                                     
REMARK   3      T33:   0.3162 T12:   0.0264                                     
REMARK   3      T13:  -0.0237 T23:   0.0433                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.5239 L22:   1.6740                                     
REMARK   3      L33:   2.0295 L12:  -0.2784                                     
REMARK   3      L13:   0.9359 L23:  -1.2965                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:   0.0369 S12:  -0.0999 S13:  -0.1091                       
REMARK   3      S21:  -0.2819 S22:   0.1255 S23:   0.2710                       
REMARK   3      S31:  -0.7232 S32:  -0.4031 S33:  -0.1441                       
REMARK   3   TLS GROUP : 6                                                      
REMARK   3    SELECTION: CHAIN 'D' AND (RESID 679 THROUGH 719 )                 
REMARK   3    ORIGIN FOR THE GROUP (A):   1.8985   1.8350 -35.8511              
REMARK   3    T TENSOR                                                          
REMARK   3      T11:   0.3459 T22:   0.2134                                     
REMARK   3      T33:   0.3597 T12:  -0.0848                                     
REMARK   3      T13:   0.0255 T23:  -0.1132                                     
REMARK   3    L TENSOR                                                          
REMARK   3      L11:   0.7861 L22:   1.7724                                     
REMARK   3      L33:   7.1942 L12:  -0.4543                                     
REMARK   3      L13:  -0.8135 L23:   0.2858                                     
REMARK   3    S TENSOR                                                          
REMARK   3      S11:  -0.1198 S12:   0.0878 S13:   0.0036                       
REMARK   3      S21:  -0.2068 S22:   0.1737 S23:  -0.0576                       
REMARK   3      S31:   1.1755 S32:  -0.0676 S33:  -0.0528                       
REMARK   3                                                                      
REMARK   3  NCS DETAILS                                                         
REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 5EOF COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 12-NOV-15.                  
REMARK 100 THE DEPOSITION ID IS D_1000215268.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 25-JUN-15                          
REMARK 200  TEMPERATURE           (KELVIN) : 298.15                             
REMARK 200  PH                             : 6.0                                
REMARK 200  NUMBER OF CRYSTALS USED        : NULL                               
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : SSRF                               
REMARK 200  BEAMLINE                       : BL17U                              
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.9791                             
REMARK 200  MONOCHROMATOR                  : NULL                               
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : ADSC QUANTUM 315                   
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : HKL-2000                           
REMARK 200  DATA SCALING SOFTWARE          : HKL-2000                           
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 19991                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.050                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 50.000                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.9                               
REMARK 200  DATA REDUNDANCY                : 6.400                              
REMARK 200  R MERGE                    (I) : NULL                               
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 14.8500                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : NULL                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : NULL                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : NULL                               
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL                               
REMARK 200  R MERGE FOR SHELL          (I) : NULL                               
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : NULL                               
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: NULL                         
REMARK 200 SOFTWARE USED: PHENIX                                                
REMARK 200 STARTING MODEL: NULL                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 48.65                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.40                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.1M MES MONOHYDRATE PH 6.0, 14% W/V     
REMARK 280  POLYETHYLENE GLYCOL 4000, VAPOR DIFFUSION, SITTING DROP,            
REMARK 280  TEMPERATURE 291.15K                                                 
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 21 21                       
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X+1/2,-Y,Z+1/2                                         
REMARK 290       3555   -X,Y+1/2,-Z+1/2                                         
REMARK 290       4555   X+1/2,-Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000       18.16050            
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       76.57050            
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       27.38750            
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000       76.57050            
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000       18.16050            
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000       27.38750            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TETRAMERIC                        
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: TETRAMERIC                 
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 9600 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 13960 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -95.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, C, D                            
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     GLY A    22                                                      
REMARK 465     PRO A    23                                                      
REMARK 465     GLY A    24                                                      
REMARK 465     SER A    25                                                      
REMARK 465     HIS A    26                                                      
REMARK 465     LEU A    27                                                      
REMARK 465     ALA A    28                                                      
REMARK 465     GLU A   103                                                      
REMARK 465     GLY B    22                                                      
REMARK 465     PRO B    23                                                      
REMARK 465     GLY B    24                                                      
REMARK 465     SER B    25                                                      
REMARK 465     HIS B    26                                                      
REMARK 465     LEU B    27                                                      
REMARK 465     ALA B    28                                                      
REMARK 465     GLU B   103                                                      
REMARK 465     GLY C   673                                                      
REMARK 465     PRO C   674                                                      
REMARK 465     GLY C   675                                                      
REMARK 465     SER C   676                                                      
REMARK 465     TYR C   677                                                      
REMARK 465     GLY C   721                                                      
REMARK 465     GLY C   722                                                      
REMARK 465     LEU C   723                                                      
REMARK 465     ARG C   724                                                      
REMARK 465     ASN C   725                                                      
REMARK 465     VAL C   726                                                      
REMARK 465     ASP C   727                                                      
REMARK 465     CYS C   728                                                      
REMARK 465     LEU C   729                                                      
REMARK 465     GLY D   673                                                      
REMARK 465     PRO D   674                                                      
REMARK 465     GLY D   675                                                      
REMARK 465     SER D   676                                                      
REMARK 465     TYR D   677                                                      
REMARK 465     PRO D   678                                                      
REMARK 465     ASP D   720                                                      
REMARK 465     GLY D   721                                                      
REMARK 465     GLY D   722                                                      
REMARK 465     LEU D   723                                                      
REMARK 465     ARG D   724                                                      
REMARK 465     ASN D   725                                                      
REMARK 465     VAL D   726                                                      
REMARK 465     ASP D   727                                                      
REMARK 465     CYS D   728                                                      
REMARK 465     LEU D   729                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER;           
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     LYS A  45    CG   CD   CE   NZ                                   
REMARK 470     HIS B  29    CG   ND1  CD2  CE1  NE2                             
REMARK 470     GLU B  38    CD   OE1  OE2                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    SER A 101       31.67    -71.19                                   
REMARK 500    THR C 718       37.53    -99.72                                   
REMARK 500    SER D 680     -137.65     47.64                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 5EOA   RELATED DB: PDB                                   
DBREF  5EOF A   26   103  UNP    Q96CV9   OPTN_HUMAN      26    103             
DBREF  5EOF B   26   103  UNP    Q96CV9   OPTN_HUMAN      26    103             
DBREF  5EOF C  677   729  UNP    Q9UHD2   TBK1_HUMAN     677    729             
DBREF  5EOF D  677   729  UNP    Q9UHD2   TBK1_HUMAN     677    729             
SEQADV 5EOF GLY A   22  UNP  Q96CV9              EXPRESSION TAG                 
SEQADV 5EOF PRO A   23  UNP  Q96CV9              EXPRESSION TAG                 
SEQADV 5EOF GLY A   24  UNP  Q96CV9              EXPRESSION TAG                 
SEQADV 5EOF SER A   25  UNP  Q96CV9              EXPRESSION TAG                 
SEQADV 5EOF GLY B   22  UNP  Q96CV9              EXPRESSION TAG                 
SEQADV 5EOF PRO B   23  UNP  Q96CV9              EXPRESSION TAG                 
SEQADV 5EOF GLY B   24  UNP  Q96CV9              EXPRESSION TAG                 
SEQADV 5EOF SER B   25  UNP  Q96CV9              EXPRESSION TAG                 
SEQADV 5EOF GLY C  673  UNP  Q9UHD2              EXPRESSION TAG                 
SEQADV 5EOF PRO C  674  UNP  Q9UHD2              EXPRESSION TAG                 
SEQADV 5EOF GLY C  675  UNP  Q9UHD2              EXPRESSION TAG                 
SEQADV 5EOF SER C  676  UNP  Q9UHD2              EXPRESSION TAG                 
SEQADV 5EOF GLY D  673  UNP  Q9UHD2              EXPRESSION TAG                 
SEQADV 5EOF PRO D  674  UNP  Q9UHD2              EXPRESSION TAG                 
SEQADV 5EOF GLY D  675  UNP  Q9UHD2              EXPRESSION TAG                 
SEQADV 5EOF SER D  676  UNP  Q9UHD2              EXPRESSION TAG                 
SEQRES   1 A   82  GLY PRO GLY SER HIS LEU ALA HIS PRO ASN LEU ASP THR          
SEQRES   2 A   82  PHE THR PRO GLU GLU LEU LEU GLN GLN MET LYS GLU LEU          
SEQRES   3 A   82  LEU THR GLU ASN HIS GLN LEU LYS GLU ALA MET LYS LEU          
SEQRES   4 A   82  ASN ASN GLN ALA MET LYS GLY ARG PHE GLU GLU LEU SER          
SEQRES   5 A   82  ALA TRP THR GLU LYS GLN LYS GLU GLU ARG GLN PHE PHE          
SEQRES   6 A   82  GLU ILE GLN SER LYS GLU ALA LYS GLU ARG LEU MET ALA          
SEQRES   7 A   82  LEU SER HIS GLU                                              
SEQRES   1 B   82  GLY PRO GLY SER HIS LEU ALA HIS PRO ASN LEU ASP THR          
SEQRES   2 B   82  PHE THR PRO GLU GLU LEU LEU GLN GLN MET LYS GLU LEU          
SEQRES   3 B   82  LEU THR GLU ASN HIS GLN LEU LYS GLU ALA MET LYS LEU          
SEQRES   4 B   82  ASN ASN GLN ALA MET LYS GLY ARG PHE GLU GLU LEU SER          
SEQRES   5 B   82  ALA TRP THR GLU LYS GLN LYS GLU GLU ARG GLN PHE PHE          
SEQRES   6 B   82  GLU ILE GLN SER LYS GLU ALA LYS GLU ARG LEU MET ALA          
SEQRES   7 B   82  LEU SER HIS GLU                                              
SEQRES   1 C   57  GLY PRO GLY SER TYR PRO SER SER ASN THR LEU VAL GLU          
SEQRES   2 C   57  MET THR LEU GLY MET LYS LYS LEU LYS GLU GLU MET GLU          
SEQRES   3 C   57  GLY VAL VAL LYS GLU LEU ALA GLU ASN ASN HIS ILE LEU          
SEQRES   4 C   57  GLU ARG PHE GLY SER LEU THR MET ASP GLY GLY LEU ARG          
SEQRES   5 C   57  ASN VAL ASP CYS LEU                                          
SEQRES   1 D   57  GLY PRO GLY SER TYR PRO SER SER ASN THR LEU VAL GLU          
SEQRES   2 D   57  MET THR LEU GLY MET LYS LYS LEU LYS GLU GLU MET GLU          
SEQRES   3 D   57  GLY VAL VAL LYS GLU LEU ALA GLU ASN ASN HIS ILE LEU          
SEQRES   4 D   57  GLU ARG PHE GLY SER LEU THR MET ASP GLY GLY LEU ARG          
SEQRES   5 D   57  ASN VAL ASP CYS LEU                                          
FORMUL   5  HOH   *98(H2 O)                                                     
HELIX    1 AA1 THR A   36  ALA A   99  1                                  64    
HELIX    2 AA2 THR B   36  HIS B  102  1                                  67    
HELIX    3 AA3 SER C  679  GLY C  715  1                                  37    
HELIX    4 AA4 SER D  680  MET D  719  1                                  40    
CRYST1   36.321   54.775  153.141  90.00  90.00  90.00 P 21 21 21    8          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.027532  0.000000  0.000000        0.00000                         
SCALE2      0.000000  0.018257  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.006530        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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