GenomeNet

Database: Pfam
Entry: BpuJI_N
LinkDB: BpuJI_N
Original site: BpuJI_N 
#=GF ID   BpuJI_N
#=GF AC   PF11564.5
#=GF DE   Restriction endonuclease BpuJI - N terminal
#=GF AU   Pollington J
#=GF SE   pdb_2vla
#=GF GA   25.00 25.00;
#=GF TC   27.20 25.80;
#=GF NC   23.80 22.60;
#=GF BM   hmmbuild HMM.ann SEED.ann
#=GF SM   hmmsearch -Z 11927849 -E 1000 --cpu 4 HMM pfamseq
#=GF TP   Family
#=GF WK   BpuJI
#=GF RN   [1]
#=GF RM   18433771
#=GF RT   The recognition domain of the BpuJI restriction endonuclease in
#=GF RT   complex with cognate DNA at 1.3-A resolution. 
#=GF RA   Sukackaite R, Grazulis S, Bochtler M, Siksnys V; 
#=GF RL   J Mol Biol. 2008;378:1084-1093.
#=GF DR   INTERPRO; IPR021108;
#=GF CC   BpuJI is a restriction endonuclease which recognises the
#=GF CC   asymmetric sequence 5'-CCCGT and cuts at multiple sites in the
#=GF CC   surrounding area of the target sequence. This family of proteins
#=GF CC   is the N terminal domain of BpuJI which has DNA recognition
#=GF CC   functions. The recognition domain has two subdomains D1 and D2.
#=GF CC   The recognition of the target sequence occurs through major
#=GF CC   groove contacts of amino acids on the helix-turn-helix region
#=GF CC   and the N-terminal arm [1].
#=GF SQ   8
#=GS K8W9T6_9ENTR/2-268  AC K8W9T6.1
#=GS E4TPW2_MARTH/7-295  AC E4TPW2.1
#=GS W8T4M6_EUBAC/1-197  AC W8T4M6.1
#=GS E5UMM8_NEIMU/2-280  AC E5UMM8.1
#=GS R7J3F2_9BACT/5-299  AC R7J3F2.1
#=GS E3PUV9_CLOSD/2-274  AC E3PUV9.1
#=GS R5SQ65_9CLOT/2-276  AC R5SQ65.1
#=GS R6CY61_9BACE/8-301  AC R6CY61.1
K8W9T6_9ENTR/2-268             ...............................YKIPTKFYQRLHHPRPRFKSNIENVLGYMSFSIAELDGETESVFKEKILSAIN..DFPGNE....GVTLKTLNNWRTEITALF..SM...VNTKNNC..NFATDLSKELANTTNLKEFFLRFISTFQYPGGFLKNNEIEKIIISDIHFHPLLW-LACFYNSSAISE.....EDLYITDVEFCHCVLNDLRVTRDHEDISATIGRILYNRKNEVNYD..........-----TAGDIKRY....ALDILDYCVLAGLMLKDYKG...KFFFRDDAKEFLSYFKDHAPIFKYYQKS-----HTLKQLESLKSEW-i.........................................................................................
E4TPW2_MARTH/7-295             ...............................YKIPDDYFFRLHHVRPRFKNDVEEVLLYVATSISEMPILDKEVFRDELNKVLF..SFKKNA....TSTQKTVDNWRTEISALF..GF...IQESNGK..QYPGVTAKRLANNQYLDEFFNYFLFSFQYPGGHIKSQNVIKQIEAGVRFKPCQFILRLLIEGEKILG.....KPFSFTAEELTQCAYFDLRVTRDGRHPKEVAELILSNRFRNLEYEhryeqlknhrTGSYPSRGDVYRY....AGDILDYMVLANLLHHKGTG..yYYYLNDQNKDAINFHLKNSVWFKEYDEFYKIDSITNPEIGTLEELWF..........................................................................................
W8T4M6_EUBAC/1-197             ..............................m-----------------------------------------------------..------....------------------..-Y...YVQD-EY..VYISERTLKFIEDTDQPAFFKDVCYKFQVPNGMQKTATVLTRIKDNLSLRPNAFVLKVLDYAKN---.....AGIRLSKKDIGYYVLNSLDVLSGKASPLEVIECILEDKKNGI---..........SRLIKTEGKASSYdyqhINEQLNYLELANLIRTDSDG...TISLNILEQKTIDVFIESLN-LPLFFSFSNYDISNKQSVSAMHTDWD..........................................................................................
E5UMM8_NEIMU/2-280             .........................fnpdiq--------YRCDIIRGKAQKELDDLLPTYSTIVADICPADENIFNQQFNNRLSqsFYQKNYnelgKNQQKTIRNHITEIAGKL.fGL...YFKKNGL..VYESPSNSKLVNDQDQPAFFKNLCLNFQFPNGTQKIQTIAERINKQIKFKPFHFILALLAEAEQ---.....VKFTITTYHIGYYVLNALEVLQGKVTPKEVLDTIISNSQKNS---..........LKRVPVTSHDFQH....IRELLNLLVLANLIVIDENRsskAIQLNHYENQLIKLFIKEYNTPLQFDIY-QFDLNAEHIGKRIEMEW-a.........................................................................................
R7J3F2_9BACT/5-299             ...............................YSIPDKDYLRIHFVRPRFKSNIENVLLYMANACCKIEPCPCREYADQINRAIR..LFPGNA....DLTQKTIDNWRTEISALC..AF...YNEDKISdlTRTSNLSFFLNENQDLTQFFKFLMLSFQFPGGHIKPSDNIELIYNGVKFKPAQFIIKVLMVGNEILSaigseKDMSISDEEATYCILNDLQVTTGKRSPKEVAQIILDNRKNNLGYYnrkdpnvfnsKGEPRTKGDVVRY....AGDIMDYMEIASLLERRMDG...YFILKPNSLQELATFINDDSYFEGYNRFYGVKHLTPSMLSPIEEQWF..........................................................................................
E3PUV9_CLOSD/2-274             ...............................YLVPNEYYVRLHHVRPRFKGDIENVLLYVAEEISKIPELPCNEFADELNNAIK..CYPGNV....NRKIKTINNWRTEISALF..GF...IESDGVT..SRAGRRAIELSEKQDLVESFKMFLFSFQYPGAHIKSHEILKLIQQGVHFKPAQYILKVIQSGEKIMG.....KKNYITKAEACHCIFNDLRCVRDNENTDLTWKRIEKNREDEIEYD..........-----MTGDIIRY....AGDIIDYMEIANLL-VSYDG..kQYYLNSLENEAILKFVKSTEWFNDYDQFISNKTASLQEINNCKKNWF..........................................................................................
R5SQ65_9CLOT/2-276             ...............................YNRLNQY--RCIIIRGKSQKEIDDLLPAYAKVIDDITPCEVEAFSQKFNELLA..PYLSAD....KRIKKTLDNHRTEIAGKLfgMY...YVEENGY..VYASERTRKFLKDNDQPAFFKDLCYKMQFPNGMQKPQTFQPLLDNKISIRPNAFVLRVLQLAAA---.....KHLVLNKKEIGYYILNSLDVLQGIAVPEEVIEAIEKDRKEGV---..........VRKIEAEGKASSYnyqhINEQINYLELANLIYTNGTG...EVWLNQNESDAIQIFASDWNKTPEFDVY-SFDLSTVEGRKQFELEWT..........................................................................................
R6CY61_9BACE/8-301             ...............................YDVPNEYWYRIHFVRPRFKSNIENVLLYMANECCRIPKCSCDEYNTRYFNAIK..MFPGNI....DMTKKTLDNWRTEIPALF..GF...YKEDKEKgiTETSQMAVFLHENQDLTQFLRLFLYSFQFPGGHMKAADVRDVIKNNIKFKPARTIIQVLMAGNEILSrnnnsKEMSMSAEEATYCIFNDVRVTNGSVSAKKIAQRILDNRKNKIKYYnpedknvlslAGKPRTKGDVTRY....AKDILDYMELANLL-TISNG...YYYLKGNEVAAIQKFKDDKTWFNGYDSFYKRNNIETKELNQIEPQWF..........................................................................................
#=GC seq_cons                  ...............................YplPscaahRlHalRPRFKssIEsVLhYhAptlscIs.tspp.FspchNpslp...a.uNh....shppKTlcNWRTEIuALh..ua...Ypc-ssp..sasSppuhcLscsQDLspFFKhhhaoFQaPGGahKspslhclIpssl+FKPspFIL+VLhtuppI.t.....KchsloccEhsYClLNDLcVspGptsPcEVhcpILcNRKssltY...........htp..ocGDlhRY....AsDILDYhELANLlhpctsG...taaLNspEpcsIphFlcctshFptYDpF.phchtohpplsslEppWh..........................................................................................
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