GenomeNet

Database: Pfam
Entry: BpuJI_N
LinkDB: BpuJI_N
Original site: BpuJI_N 
#=GF ID   BpuJI_N
#=GF AC   PF11564.6
#=GF DE   Restriction endonuclease BpuJI - N terminal
#=GF AU   Pollington J
#=GF SE   pdb_2vla
#=GF GA   25.00 25.00;
#=GF TC   25.60 25.80;
#=GF NC   23.80 24.20;
#=GF BM   hmmbuild HMM.ann SEED.ann
#=GF SM   hmmsearch -Z 17690987 -E 1000 --cpu 4 HMM pfamseq
#=GF TP   Family
#=GF WK   BpuJI
#=GF RN   [1]
#=GF RM   18433771
#=GF RT   The recognition domain of the BpuJI restriction endonuclease in
#=GF RT   complex with cognate DNA at 1.3-A resolution. 
#=GF RA   Sukackaite R, Grazulis S, Bochtler M, Siksnys V; 
#=GF RL   J Mol Biol. 2008;378:1084-1093.
#=GF DR   INTERPRO; IPR021108;
#=GF CC   BpuJI is a restriction endonuclease which recognises the
#=GF CC   asymmetric sequence 5'-CCCGT and cuts at multiple sites in the
#=GF CC   surrounding area of the target sequence. This family of proteins
#=GF CC   is the N terminal domain of BpuJI which has DNA recognition
#=GF CC   functions. The recognition domain has two subdomains D1 and D2.
#=GF CC   The recognition of the target sequence occurs through major
#=GF CC   groove contacts of amino acids on the helix-turn-helix region
#=GF CC   and the N-terminal arm [1].
#=GF SQ   14
#=GS E5UMM8_NEIMU/2-280      AC E5UMM8.1
#=GS E3PUV9_CLOSD/2-274      AC E3PUV9.1
#=GS A0A0F0CRJ4_9BACT/1-272  AC A0A0F0CRJ4.1
#=GS R6CY61_9BACE/8-301      AC R6CY61.1
#=GS F5S6P5_9NEIS/5-298      AC F5S6P5.1
#=GS A0A0G1JVU9_9BACT/4-272  AC A0A0G1JVU9.1
#=GS A0A0G0V7D2_9BACT/3-271  AC A0A0G0V7D2.1
#=GS K8W9T6_9ENTR/2-268      AC K8W9T6.1
#=GS W8T4M6_EUBAC/1-197      AC W8T4M6.1
#=GS E4TPW2_MARTH/7-295      AC E4TPW2.1
#=GS A0A0G1VFT2_9BACT/4-277  AC A0A0G1VFT2.1
#=GS R7J3F2_9BACT/5-299      AC R7J3F2.1
#=GS R5SQ65_9CLOT/2-276      AC R5SQ65.1
#=GS E9S8F5_RUMAL/1-129      AC E9S8F5.1
E5UMM8_NEIMU/2-280                 .........................fnpdiq--------YRCDIIRGKAQKELDDLLPTYSTIVADICPADENIFNQQFNNRLSqsFYQKNYnelgKNQQKTIRNHITE.IAGKL.fGL...YF.KKNGL..VYESPSNSKLVNDQDQPAFFKNLCLNFQFPNGTQKIQTIAERINKQIKFK.....PFHFILALLAEAEQ---.....VKFTITTYHIGYYVL.....NALEVLQGKV.TPKEVLDTIISNSQKNS---..........LKRVPVTSHDFQH....IRELLNLLVLANLIVIDENRsskAIQLNHYENQLIKLFIKEYNTPLQFDIY-QFDLNAEHIGKRIEMEW-a.........................................................................................
E3PUV9_CLOSD/2-274                 ...............................YLVPNEYYVRLHHVRPRFKGDIENVLLYVAEEISKIPELPCNEFADELNNAIK..CYPGNV....NRKIKTINNWRTE.ISALF..GF...IE.SDGVT..SRAGRRAIELSEKQDLVESFKMFLFSFQYPGAHIKSHEILKLIQQGVHFK.....PAQYILKVIQSGEKIMG.....KKNYITKAEACHCIF.....NDLRCVRDNE.NTDLTWKRIEKNREDEIEYD..........-----MTGDIIRY....AGDIIDYMEIANLL-VSYDG..kQYYLNSLENEAILKFVKSTEWFNDYDQFISNKTASLQEINNCKKNWF..........................................................................................
A0A0F0CRJ4_9BACT/1-272             ..............................m---PREHFYRIHHIRPRFKNDIESVLIFMATEISKIGEKPSDIFAESVNAAIK..RYPGNI....TKKEKTINNWRTE.ISSLF..GL...FQ.TDSGRnvSFPGLRAVELADRQDLVLFFKLFLYHFQYPGGHLKPHENAELIRQGVHFR.....PAQHILKMLLAVEEKTK.....QRVWINKAEATHCIF.....NDLRVIRDGE.DVLDTWKRISKNRQDRVEYD..........-----WKGDVIRY....AGDILDYMEIANLV--VCHG..tQFFANSLEKAAVLKVVSSDDWFSGYDAFIKARAVSTPEVSALQEDW-y.........................................................................................
R6CY61_9BACE/8-301                 ...............................YDVPNEYWYRIHFVRPRFKSNIENVLLYMANECCRIPKCSCDEYNTRYFNAIK..MFPGNI....DMTKKTLDNWRTE.IPALF..GF...YK.EDKEKgiTETSQMAVFLHENQDLTQFLRLFLYSFQFPGGHMKAADVRDVIKNNIKFK.....PARTIIQVLMAGNEILSrnnnsKEMSMSAEEATYCIF.....NDVRVTNGSV.SAKKIAQRILDNRKNKIKYYnpedknvlslAGKPRTKGDVTRY....AKDILDYMELANLL-TISNG...YYYLKGNEVAAIQKFKDDKTWFNGYDSFYKRNNIETKELNQIEPQWF..........................................................................................
F5S6P5_9NEIS/5-298                 ...............................YQIPEEYFFRLHHIRPRFKSNVEEVLLYVANSISDLGKLPEKAFNEQLNAVLR..NFVKNQ....TAEQKTINNWRTE.IAALF..SF...IQ.ETENN.eLLPSKMAKRLAENQFLDEFFNYFLYSFQYPAGHNKSHAVIAQIQNGIQFRpcqfrPCQFILQIFQAACKLSD.....KPFYLTAEELTQCAY.....FDLRVTSHQTkTANDVAKQIIENRENKVSYNhkye.qlkteSGNYPSSGDVYRY....AGDILDYMVLANLLKTKGTG...HYYLNTDNTDFIKRHLDNTAWFNQYADFYGKQEISAREITVLEKKWF..........................................................................................
A0A0G1JVU9_9BACT/4-272             ...............................YQTPTKYLLRCAFPRGRILSQIEDELSILTQFVSRFTPKDKEQFDTLIDS---..EYAKLR....SVSAKSIKNYRTE.MTKLF..GL...ITvGSDGV..VQASERTNLLVESQNFPLFFKTFCHRFQFPNGINKPQETAKQIEAGAQFK.....PAKFILDLFVLGVEKCG.....QDFSINGNEISNLVF.....NDVRVTSGKM.SPHQVLDFLLKLRSGHVRFA..........-----GGSKIAQH....GREFLGYMLLARLLKEGEN-...GFRLNEKERQAIDYIRQSELFFNVPRDF-ATNTSTRKQLQ-------yewglwf...................................................................................
A0A0G0V7D2_9BACT/3-271             .........................fnpdiq--------YRCTIIRGKSISRMDDYLPIYAEILNEICPIPADQFDNTFDKKLS..HYIKDD....---EKTIRNHRTEnVDKLL..GM...YF.EKDEI..IYTSERTKKFLEDNDQPAFFKSVCYKFQQPNGSQKLQTTKEKIENEISLK.....PYHFVLALLKTAAI---.....RKIILNKNEVAYYVL.....NALQVLQGKV.TVDEVLKAILEDRERGIEKK..........VDISKNYAWDYRH....INEQFELLALTNLI--RKDG..kSVWLNTRELSSIDFFIEDLK-KPLAIDFSKFDLHEKNIEKKMKIDW-q.........................................................................................
K8W9T6_9ENTR/2-268                 ...............................YKIPTKFYQRLHHPRPRFKSNIENVLGYMSFSIAELDGETESVFKEKILSAIN..DFPGNE....GVTLKTLNNWRTE.ITALF..SM...VN.TKNNC..NFATDLSKELANTTNLKEFFLRFISTFQYPGGFLKNNEIEKIIISDIHFH.....PLLW-LACFYNSSAISE.....EDLYITDVEFCHCVL.....NDLRVTRDHE.DISATIGRILYNRKNEVNYD..........-----TAGDIKRY....ALDILDYCVLAGLMLKDYKG...KFFFRDDAKEFLSYFKDHAPIFKYYQKS-----HTLKQLESLKSEW-i.........................................................................................
W8T4M6_EUBAC/1-197                 ..............................m-----------------------------------------------------..------....-------------.-----..-Y...YV.QD-EY..VYISERTLKFIEDTDQPAFFKDVCYKFQVPNGMQKTATVLTRIKDNLSLR.....PNAFVLKVLDYAKN---.....AGIRLSKKDIGYYVL.....NSLDVLSGKA.SPLEVIECILEDKKNGI---..........SRLIKTEGKASSYdyqhINEQLNYLELANLIRTDSDG...TISLNILEQKTIDVFIESLN-LPLFFSFSNYDISNKQSVSAMHTDWD..........................................................................................
E4TPW2_MARTH/7-295                 ...............................YKIPDDYFFRLHHVRPRFKNDVEEVLLYVATSISEMPILDKEVFRDELNKVLF..SFKKNA....TSTQKTVDNWRTE.ISALF..GF...IQ.ESNGK..QYPGVTAKRLANNQYLDEFFNYFLFSFQYPGGHIKSQNVIKQIEAGVRFK.....PCQFILRLLIEGEKILG.....KPFSFTAEELTQCAY.....FDLRVTRDGR.HPKEVAELILSNRFRNLEYEhryeqlknhrTGSYPSRGDVYRY....AGDILDYMVLANLLHHKGTG..yYYYLNDQNKDAINFHLKNSVWFKEYDEFYKIDSITNPEIGTLEELWF..........................................................................................
A0A0G1VFT2_9BACT/4-277             ...............................YKIPEEFYYRIHHARPRFKSDVESVLLFMAGEISRLGPAPRSPFREKLNSAIR..LYPGNG....TATEKTIDNWRTE.ISSLF..GL...IE.REGDL..QKAGRMAFLLSENQDLVEFFRYFLYYFQYPGGHLKSQESLKMINAGIKFK.....PAKYLIEVLIEGQK-LSe...gGKFGISKAEATHCIF.....NDLRITRDGQ.SPKVTAAQIIARQKTGVEYD..........-----NGGDVTRY....AGDILDYMELADLTRQRPNY...KYYLNMANLDVLQAFVSSDNWFSSYDALYGQKDLTPADVAVYLEDWF..........................................................................................
R7J3F2_9BACT/5-299                 ...............................YSIPDKDYLRIHFVRPRFKSNIENVLLYMANACCKIEPCPCREYADQINRAIR..LFPGNA....DLTQKTIDNWRTE.ISALC..AF...YN.EDKISdlTRTSNLSFFLNENQDLTQFFKFLMLSFQFPGGHIKPSDNIELIYNGVKFK.....PAQFIIKVLMVGNEILSaigseKDMSISDEEATYCIL.....NDLQVTTGKR.SPKEVAQIILDNRKNNLGYYnrkdpnvfnsKGEPRTKGDVVRY....AGDIMDYMEIASLLERRMDG...YFILKPNSLQELATFINDDSYFEGYNRFYGVKHLTPSMLSPIEEQWF..........................................................................................
R5SQ65_9CLOT/2-276                 ...............................YNRLNQY--RCIIIRGKSQKEIDDLLPAYAKVIDDITPCEVEAFSQKFNELLA..PYLSAD....KRIKKTLDNHRTE.IAGKLfgMY...YV.EENGY..VYASERTRKFLKDNDQPAFFKDLCYKMQFPNGMQKPQTFQPLLDNKISIR.....PNAFVLRVLQLAAA---.....KHLVLNKKEIGYYIL.....NSLDVLQGIA.VPEEVIEAIEKDRKEGV---..........VRKIEAEGKASSYnyqhINEQINYLELANLIYTNGTG...EVWLNQNESDAIQIFASDWNKTPEFDVY-SFDLSTVEGRKQFELEWT..........................................................................................
E9S8F5_RUMAL/1-129                 ..........................mlaky-----------------------------------------------------..------....-------------.-----..--...--.-----..--------------------------------------------------.....-----------------.....ANITLTKSDIGYYVL.....NNLDVLQRKA.TPAEVIEQIDKDRKNRI---..........ERKVHTDGKASSYdvqhINEQINYLELANLV-IIDDK...DVSINPNEMETVQMFADEYNSDPMFDCS-AYNFDTVEGRKLFSQKW-n.........................................................................................
#=GC seq_cons                      ...............................Y.hPpcahhRhcalRsRhpsplEslL.hhAp.lscls.hspp.Fspphsphlp...a.tN.....shppKTlsNaRTE.IsuLh..uh...hp.pcssh..shsophshcLs-sQDhstFF+hhhapFQaPsGp.KsppshchIpsslpF+.....PspalLplLhtupp..t.....pchhlocpEhsahlh.....NDLcVhpspt.osc-VhcpIlcsRcsplpa............t...spGclhRY....us-lL-YhtLANLl.pptsG...paaLNspEppsIphFlcstshFstacsF.thchhohpplpthcpcW...........................................................................................
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