GenomeNet

Database: Pfam
Entry: BpuJI_N
LinkDB: BpuJI_N
Original site: BpuJI_N 
#=GF ID   BpuJI_N
#=GF AC   PF11564.7
#=GF DE   Restriction endonuclease BpuJI - N terminal
#=GF AU   Pollington J
#=GF SE   pdb_2vla
#=GF GA   25.00 25.00;
#=GF TC   25.60 25.80;
#=GF NC   23.80 24.20;
#=GF BM   hmmbuild HMM.ann SEED.ann
#=GF SM   hmmsearch -Z 26740544 -E 1000 --cpu 4 HMM pfamseq
#=GF TP   Family
#=GF WK   BpuJI
#=GF RN   [1]
#=GF RM   18433771
#=GF RT   The recognition domain of the BpuJI restriction endonuclease in
#=GF RT   complex with cognate DNA at 1.3-A resolution. 
#=GF RA   Sukackaite R, Grazulis S, Bochtler M, Siksnys V; 
#=GF RL   J Mol Biol. 2008;378:1084-1093.
#=GF DR   INTERPRO; IPR021108;
#=GF CC   BpuJI is a restriction endonuclease which recognises the
#=GF CC   asymmetric sequence 5'-CCCGT and cuts at multiple sites in the
#=GF CC   surrounding area of the target sequence. This family of proteins
#=GF CC   is the N terminal domain of BpuJI which has DNA recognition
#=GF CC   functions. The recognition domain has two subdomains D1 and D2.
#=GF CC   The recognition of the target sequence occurs through major
#=GF CC   groove contacts of amino acids on the helix-turn-helix region
#=GF CC   and the N-terminal arm [1].
#=GF SQ   24
#=GS A0A133PZD2_9BACT/9-302   AC A0A133PZD2.1
#=GS E5UMM8_NEIMU/2-280       AC E5UMM8.1
#=GS E3PUV9_CLOSD/2-274       AC E3PUV9.1
#=GS R9JXU0_9FIRM/4-276       AC R9JXU0.1
#=GS A0A150YGM7_9BACI/46-222  AC A0A150YGM7.1
#=GS A0A0F0CRJ4_9BACT/1-272   AC A0A0F0CRJ4.1
#=GS R6CY61_9BACE/8-301       AC R6CY61.1
#=GS A0A096ASW7_9FIRM/49-162  AC A0A096ASW7.1
#=GS F5S6P5_9NEIS/5-298       AC F5S6P5.1
#=GS A0A0G1JVU9_9BACT/4-272   AC A0A0G1JVU9.1
#=GS A0A0B0HJJ2_9BACI/4-123   AC A0A0B0HJJ2.1
#=GS A0A0G0V7D2_9BACT/3-271   AC A0A0G0V7D2.1
#=GS K8W9T6_9GAMM/2-268       AC K8W9T6.1
#=GS W8T4M6_EUBAC/1-197       AC W8T4M6.1
#=GS E4TPW2_MARTH/7-295       AC E4TPW2.1
#=GS A0A136HVS8_9ALTE/2-275   AC A0A136HVS8.1
#=GS A0A0G1VFT2_9BACT/4-277   AC A0A0G1VFT2.1
#=GS R7J3F2_9BACT/5-299       AC R7J3F2.1
#=GS A0A0B0HML8_9BACI/1-151   AC A0A0B0HML8.1
#=GS F0F9L7_9BACT/2-284       AC F0F9L7.1
#=GS R5SQ65_9CLOT/2-276       AC R5SQ65.1
#=GS A0A0K9XYK8_9FLAO/7-295   AC A0A0K9XYK8.1
#=GS A0A0D6AJE0_9CHRO/1-248   AC A0A0D6AJE0.1
#=GS E9S8F5_RUMAL/1-129       AC E9S8F5.1
A0A133PZD2_9BACT/9-302              ...................................YNLPDKYWHRIHFVRPRFKSNIENVLLYMAGECCRIPDCSCEDYNKKYLNAI.......R..MFPGNI....DMAEKTLQNWRTE.IPALF..GF...Y...V.E.DKEAdiTRTSKMATFLYENQDLTQFFRLFLMSFQFPGGHMKPQDLKDIIYLNIRFK.....PAKTIIQILLAGNELLSsensiKEMSLSAEEATYCIF.....NDVRVTSG.Q..I.SPKQVAKTILDNRK.NQIKYYnpadqhtkslTGASRTKG.DMTRY....AGDILDYMELADLL-T-KNG..sYFYLKGNELQAIQAFAKDKT.WFKGYESFYGQNDLDTASLSAVEPNWF..........................................................................................
E5UMM8_NEIMU/2-280                  .............................fnpdiq--------YRCDIIRGKAQKELDDLLPTYSTIVADICPADENIFNQQFNNRL.......SqsFYQKNYnelgKNQQKTIRNHITE.IAGKL.fGL...Y...F.K.KNGL..VYESPSNSKLVNDQDQPAFFKNLCLNFQFPNGTQKIQTIAERINKQIKFK.....PFHFILALLAEAEQ---.....VKFTITTYHIGYYVL.....NALEVLQG.K..V.TPKEVLDTIISNSQ.KNS---..........LKRVPVTS.HDFQH....IRELLNLLVLANLIVIDENRsskAIQLNHYENQLIKLFIKEYN.TPLQFDIY-QFDLNAEHIGKRIEMEW-a.........................................................................................
E3PUV9_CLOSD/2-274                  ...................................YLVPNEYYVRLHHVRPRFKGDIENVLLYVAEEISKIPELPCNEFADELNNAI.......K..CYPGNV....NRKIKTINNWRTE.ISALF..GF...I...E.S.DGVT..SRAGRRAIELSEKQDLVESFKMFLFSFQYPGAHIKSHEILKLIQQGVHFK.....PAQYILKVIQSGEKIMG.....KKNYITKAEACHCIF.....NDLRCVRD.N..E.NTDLTWKRIEKNRE.DEIEYD..........-----MTG.DIIRY....AGDIIDYMEIANLL-VSYDG..kQYYLNSLENEAILKFVKSTE.WFNDYDQFISNKTASLQEINNCKKNWF..........................................................................................
R9JXU0_9FIRM/4-276                  ...................................YQIPEQYYFRLHHVRPRFKGDIENVLIYVAEEITRVGERATDEFVEAVNSAL.......F..RYPGNA....NRKLKTINNWRTE.ISALF..GF...I...E.H.TDTT..DKPGWRAVELAENQDLVEGFKVFLYNFQYPGAHIKPKEVLEQIKQGVHFK.....PAQYILKMLRYANREEG.....NSIGITKPEVCHCIF.....NDLRVTRD.Q..E.GIERTWSRISYNRR.QRMTYD..........-----ETG.DVIRY....AGDIIDYMEIANLLKTYDSR...TYYLNTLEEDSIVKFCESEE.WFGEYDAMIANRSGSMKEVKDCINGWF..........................................................................................
A0A150YGM7_9BACI/46-222             hfeqvlankglkrnnisndgsggrtwcamlktfay----------------------------------------------------.......-..------....-------------.-----..-C...Y...I.D.HSGY..VKPTKVGKALINNNKEFENVKKQILTLQIPNSYFLESGFRPRFASSFKIR.....PARFLIKIVHEARL---.....-DFYVTKEEITYFVL.....TA----QT.D..S.QVLQVVEKILAYRNsNDV---..........--------.-----....--------------------...--------------------.---------------------------erelmkqevavdfdhrarndhvardyfqahsd..........................................................
A0A0F0CRJ4_9BACT/1-272              ..................................m---PREHFYRIHHIRPRFKNDIESVLIFMATEISKIGEKPSDIFAESVNAAI.......K..RYPGNI....TKKEKTINNWRTE.ISSLF..GL...F...Q.T.DSGRnvSFPGLRAVELADRQDLVLFFKLFLYHFQYPGGHLKPHENAELIRQGVHFR.....PAQHILKMLLAVEEKTK.....QRVWINKAEATHCIF.....NDLRVIRD.G..E.DVLDTWKRISKNRQ.DRVEYD..........-----WKG.DVIRY....AGDILDYMEIANLV--VCHG..tQFFANSLEKAAVLKVVSSDD.WFSGYDAFIKARAVSTPEVSALQEDW-y.........................................................................................
R6CY61_9BACE/8-301                  ...................................YDVPNEYWYRIHFVRPRFKSNIENVLLYMANECCRIPKCSCDEYNTRYFNAI.......K..MFPGNI....DMTKKTLDNWRTE.IPALF..GF...Y...K.E.DKEKgiTETSQMAVFLHENQDLTQFLRLFLYSFQFPGGHMKAADVRDVIKNNIKFK.....PARTIIQVLMAGNEILSrnnnsKEMSMSAEEATYCIF.....NDVRVTNG.S..V.SAKKIAQRILDNRK.NKIKYYnpedknvlslAGKPRTKG.DVTRY....AKDILDYMELANLL-TISNG...YYYLKGNEVAAIQKFKDDKT.WFNGYDSFYKRNNIETKELNQIEPQWF..........................................................................................
A0A096ASW7_9FIRM/49-162             ..................ryfslykgsnnleeaef----------------------------------------------------.......-..------....-------------.-----..--...-...-.-.----..-------------GDSRNVVLNFMSKFQFPNDR-KKADFDTQVNQNIRLK.....PYQVIVKLLYIGSL-SD.....SNFYLTQEEIFLFIF.....LNPEVATTqS..I.DYSKLLRDVLDYRG.NH----..........--------.-----....--------------------...--------------------.---------------------------iip.......................................................................................
F5S6P5_9NEIS/5-298                  ...................................YQIPEEYFFRLHHIRPRFKSNVEEVLLYVANSISDLGKLPEKAFNEQLNAVL.......R..NFVKNQ....TAEQKTINNWRTE.IAALF..SF...I...Q.E.TENN.eLLPSKMAKRLAENQFLDEFFNYFLYSFQYPAGHNKSHAVIAQIQNGIQFRpcqfrPCQFILQIFQAACKLSD.....KPFYLTAEELTQCAY.....FDLRVTSH.Q..TkTANDVAKQIIENRE.NKVSYNhkye.qlkteSGNYPSSG.DVYRY....AGDILDYMVLANLLKTKGTG...HYYLNTDNTDFIKRHLDNTA.WFNQYADFYGKQEISAREITVLEKKWF..........................................................................................
A0A0G1JVU9_9BACT/4-272              ...................................YQTPTKYLLRCAFPRGRILSQIEDELSILTQFVSRFTPKDKEQFDTLIDS--.......-..EYAKLR....SVSAKSIKNYRTE.MTKLF..GL...I...TvG.SDGV..VQASERTNLLVESQNFPLFFKTFCHRFQFPNGINKPQETAKQIEAGAQFK.....PAKFILDLFVLGVEKCG.....QDFSINGNEISNLVF.....NDVRVTSG.K..M.SPHQVLDFLLKLRS.GHVRFA..........-----GGS.KIAQH....GREFLGYMLLARLLKEGEN-...GFRLNEKERQAIDYIRQSEL.FFNVPRDF-ATNTSTRKQLQ-------yewglwf...................................................................................
A0A0B0HJJ2_9BACI/4-123              ...................................YQVPEQYYFRIHHPRPRFKNDIENVLIYLATEITAIGKKIATDFTKELNAAI.......R..RYPGNL....TKKQKTIDNWRTE.IDALF..CF...I...E.R.EDIY..LKPSRRAVELATTQDLVKFFKLFCYHFS----------------------.....-----------------.....---------------.....--------.-..-.--------------.------..........--------.-----....--------------------...--------------------.---------------------------vswrfs....................................................................................
A0A0G0V7D2_9BACT/3-271              .............................fnpdiq--------YRCTIIRGKSISRMDDYLPIYAEILNEICPIPADQFDNTFDKKL.......S..HYIKDD....---EKTIRNHRTEnVDKLL..GM...Y...F.E.KDEI..IYTSERTKKFLEDNDQPAFFKSVCYKFQQPNGSQKLQTTKEKIENEISLK.....PYHFVLALLKTAAI---.....RKIILNKNEVAYYVL.....NALQVLQG.K..V.TVDEVLKAILEDRE.RGIEKK..........VDISKNYA.WDYRH....INEQFELLALTNLI--RKDG..kSVWLNTRELSSIDFFIEDLK.-KPLAIDFSKFDLHEKNIEKKMKIDW-q.........................................................................................
K8W9T6_9GAMM/2-268                  ...................................YKIPTKFYQRLHHPRPRFKSNIENVLGYMSFSIAELDGETESVFKEKILSAI.......N..DFPGNE....GVTLKTLNNWRTE.ITALF..SM...V...N.T.KNNC..NFATDLSKELANTTNLKEFFLRFISTFQYPGGFLKNNEIEKIIISDIHFH.....PLLW-LACFYNSSAISE.....EDLYITDVEFCHCVL.....NDLRVTRD.H..E.DISATIGRILYNRK.NEVNYD..........-----TAG.DIKRY....ALDILDYCVLAGLMLKDYKG...KFFFRDDAKEFLSYFKDHAP.IFKYYQKS-----HTLKQLESLKSEW-i.........................................................................................
W8T4M6_EUBAC/1-197                  ..................................m----------------------------------------------------.......-..------....-------------.-----..-Y...Y...V.Q.D-EY..VYISERTLKFIEDTDQPAFFKDVCYKFQVPNGMQKTATVLTRIKDNLSLR.....PNAFVLKVLDYAKN---.....AGIRLSKKDIGYYVL.....NSLDVLSG.K..A.SPLEVIECILEDKK.NGI---..........SRLIKTEG.KASSYdyqhINEQLNYLELANLIRTDSDG...TISLNILEQKTIDVFIESLN.-LPLFFSFSNYDISNKQSVSAMHTDWD..........................................................................................
E4TPW2_MARTH/7-295                  ...................................YKIPDDYFFRLHHVRPRFKNDVEEVLLYVATSISEMPILDKEVFRDELNKVL.......F..SFKKNA....TSTQKTVDNWRTE.ISALF..GF...I...Q.E.SNGK..QYPGVTAKRLANNQYLDEFFNYFLFSFQYPGGHIKSQNVIKQIEAGVRFK.....PCQFILRLLIEGEKILG.....KPFSFTAEELTQCAY.....FDLRVTRD.G..R.HPKEVAELILSNRF.RNLEYEhryeqlknhrTGSYPSRG.DVYRY....AGDILDYMVLANLLHHKGTG..yYYYLNDQNKDAINFHLKNSV.WFKEYDEFYKIDSITNPEIGTLEELWF..........................................................................................
A0A136HVS8_9ALTE/2-275              ...................................YRIPEEFFVRLHHCRPRFKNDRENVLLFIASELCLIGELPKADFAKELNSAI.......K..RFPGNS....AKKSKTIDNWRTE.ISSLL..GL...I...E.YtEDDT..CKPSQMAEILNNGQDLIEFFRFFCYKFQYPGGHLKPKESLELIKLGVKFK.....PVRYILKLLVEANKNSD.....KKFGISKEEATHCIF.....NDLRVVRD.D..R.AVEDTISIILANRS.SGIEYD..........-----GNG.DIIRY....AGDILDYMVLANLLDYKPNG...KYYIRTVEPEVINTICHDDN.YFPEYEPLYNRDDLELQDITTTQLAWF..........................................................................................
A0A0G1VFT2_9BACT/4-277              ...................................YKIPEEFYYRIHHARPRFKSDVESVLLFMAGEISRLGPAPRSPFREKLNSAI.......R..LYPGNG....TATEKTIDNWRTE.ISSLF..GL...I...E.R.EGDL..QKAGRMAFLLSENQDLVEFFRYFLYYFQYPGGHLKSQESLKMINAGIKFK.....PAKYLIEVLIEGQK-LSe...gGKFGISKAEATHCIF.....NDLRITRD.G..Q.SPKVTAAQIIARQK.TGVEYD..........-----NGG.DVTRY....AGDILDYMELADLTRQRPNY...KYYLNMANLDVLQAFVSSDN.WFSSYDALYGQKDLTPADVAVYLEDWF..........................................................................................
R7J3F2_9BACT/5-299                  ...................................YSIPDKDYLRIHFVRPRFKSNIENVLLYMANACCKIEPCPCREYADQINRAI.......R..LFPGNA....DLTQKTIDNWRTE.ISALC..AF...Y...N.E.DKISdlTRTSNLSFFLNENQDLTQFFKFLMLSFQFPGGHIKPSDNIELIYNGVKFK.....PAQFIIKVLMVGNEILSaigseKDMSISDEEATYCIL.....NDLQVTTG.K..R.SPKEVAQIILDNRK.NNLGYYnrkdpnvfnsKGEPRTKG.DVVRY....AGDIMDYMEIASLLERRMDG...YFILKPNSLQELATFINDDS.YFEGYNRFYGVKHLTPSMLSPIEEQWF..........................................................................................
A0A0B0HML8_9BACI/1-151              .................................mn----------------------------------------------------.......-..------....-------------.-----..--...-...-.-.----..---------------------------------------LEYLRQGIKFR.....PCSYVLKMLYTAEKNSG.....NRAGITKAEATHCVF.....NDLRVTRD.N..R.CVEDTWDLVMHNRR.NNIEYD..........-----WTG.DVIRY....AGDILDYMVQANLLVKHADG...RYYINHVEDIAIQRFINPTSdLFDYYEKIKDISNITIDDVKDLDDEW-v.........................................................................................
F0F9L7_9BACT/2-284                  ..............................ydptl-----Q--YRCTIIRGKSQTDMEDLLPLYANIVHKYCPCEEKQFRASCCKALskalfstE..AYDRLS....DSNQKTVLNHLTE.IAGTLlgLY...YpayD.D.ETKTnyINESDSCKYLIESGDYPTFFKNLCLNFQFPNGAKWLQFIEDDIKNKIRIK.....PFCYVVSLLFHAQQQVE.....R-ELLTKQEIGFYVL.....NSLEVLQG.K..V.PCSDVYDRIMADRK.NKV---..........-RRERLSGsHDWQH....IKEQFNLLELADIIETDAT-...YIWLNKNAAESIACFIKHKD.DF-IFDAY-KYDLKSKAGRIQYMEDW-k.........................................................................................
R5SQ65_9CLOT/2-276                  ...................................YNRLNQY--RCIIIRGKSQKEIDDLLPAYAKVIDDITPCEVEAFSQKFNELL.......A..PYLSAD....KRIKKTLDNHRTE.IAGKLfgMY...Y...V.E.ENGY..VYASERTRKFLKDNDQPAFFKDLCYKMQFPNGMQKPQTFQPLLDNKISIR.....PNAFVLRVLQLAAA---.....KHLVLNKKEIGYYIL.....NSLDVLQG.I..A.VPEEVIEAIEKDRK.EGV---..........VRKIEAEG.KASSYnyqhINEQINYLELANLIYTNGTG...EVWLNQNESDAIQIFASDWN.KTPEFDVY-SFDLSTVEGRKQFELEWT..........................................................................................
A0A0K9XYK8_9FLAO/7-295              ...................................YKIPDEYFFRLHHVRPRFKNDVEEVLLYVATSISEMTVLPEKEFNDELNKVL.......F..GFNKNS....TSTKKTIDNWRTE.ISALF..AF...I...Q.E.TEGH..LQPGITAKRLANNQYLDEFFNYFLFSFQYPGGHIKSQNVIKQIEAGVRFK.....PCQFILQLLIEGEKLTG.....KPFSITAEELTQCAY.....FDLRVTRD.N..R.HPKLVAELILENRA.HNVEYEhkyqslwnekTQSFPSKG.DVYRY....AGDILDYMVLANLLNHKGTG..yYYYLNSENKEAINFHLSHLV.WFNEYDKYYEAENISTSEIGALEEIWF..........................................................................................
A0A0D6AJE0_9CHRO/1-248              ...................................----------------------------MATCLSNLSEMPIDKFAKAMNNAI.......K..LFPGNA....NKKDKTINNWRTE.ISALF..GL...I...E.T.NSINqkSKPSNLAFRLSENQDLVEFFKYFLFYFQYPGGYLKPHEILDYINQGILFK.....PVSYLIKVLQEGEKITE.....GRFYIDKAEATHCIF.....NDLRVTRQ.NnpR.KPKNTAYLVIHNRK.NNINYN..........-----WTG.DVIRY....AGDILDYMVIADLL--VCHG..nKFYLNNKNQEVIVSFIENEV.IFLDYIPLYKKLNLNISDIKSLNDAWF..........................................................................................
E9S8F5_RUMAL/1-129                  ..............................mlaky----------------------------------------------------.......-..------....-------------.-----..--...-...-.-.----..--------------------------------------------------.....-----------------.....ANITLTKSDIGYYVL.....NNLDVLQR.K..A.TPAEVIEQIDKDRK.NRI---..........ERKVHTDG.KASSYdvqhINEQINYLELANLV-IIDDK...DVSINPNEMETVQMFADEYN.SDPMFDCS-AYNFDTVEGRKLFSQKW-n.........................................................................................
#=GC seq_cons                       .......................................pp..hRhphhRs+hpsplEslL.hhAp.lscls.hspp.Fspphspsl.......t...astN.....s.ppKTlpNaRTE.IsuLh..uh...h...p.p.csth...hsophuhhLhpsQDhspFF+hFhapFQaPsGt.KspphhchIppsl+FK.....PspallplLhtupp..t.....pchhlocpEhsahlh.....NDLcVsps.p..t.sscclhctIlcsRc.splpa................spG.cshRY....us-ll-Yh.LAsLl.httsG...taaLNp.p.psIphFhpsts.hF..acth.thp.hshpplpthp.pW...........................................................................................
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