GenomeNet

Database: Pfam
Entry: EcoRII-N
LinkDB: EcoRII-N
Original site: EcoRII-N 
#=GF ID   EcoRII-N
#=GF AC   PF09217.7
#=GF DE   Restriction endonuclease EcoRII, N-terminal
#=GF AU   Sammut SJ
#=GF SE   pdb_1na6
#=GF GA   25.00 25.00;
#=GF TC   25.50 26.30;
#=GF NC   21.10 24.80;
#=GF BM   hmmbuild HMM.ann SEED.ann
#=GF SM   hmmsearch -Z 11927849 -E 1000 --cpu 4 HMM pfamseq
#=GF TP   Domain
#=GF WK   R.EcoRII
#=GF RN   [1]
#=GF RM   14659759
#=GF RT   Crystal structure of type IIE restriction endonuclease EcoRII
#=GF RT   reveals an autoinhibition mechanism by a novel effector-binding
#=GF RT   fold. 
#=GF RA   Zhou XE, Wang Y, Reuter M, Mucke M, Kruger DH, Meehan EJ, Chen
#=GF RA   L; 
#=GF RL   J Mol Biol. 2004;335:307-319.
#=GF DR   INTERPRO; IPR023372;
#=GF DR   SCOP; 1na6; fa;
#=GF CC   The N-terminal effector-binding domain of the Restriction
#=GF CC   Endonuclease EcoRII has a DNA recognition fold, allowing for
#=GF CC   binding to 5'-CCWGG sequences. It assumes a structure composed
#=GF CC   of an eight-stranded beta-sheet with the strands in the order of
#=GF CC   b2, b5, b4, b3, b7, b6, b1 and b8. They are mostly antiparallel
#=GF CC   to each other except that b3 is parallel to b7. Alternatively,
#=GF CC   it may also be viewed as consisting of two mini beta-sheets of
#=GF CC   four antiparallel beta-strands, sheet I from beta-strands b2,
#=GF CC   b5, b4, b3 and sheet II from strands b7, b6, b1, b8, folded into
#=GF CC   an open mixed beta-barrel with a novel topology. Sheet I has a
#=GF CC   simple Greek key motif while sheet II does not [1].
#=GF SQ   18
#=GS J9ZE30_LEPFM/14-167      AC J9ZE30.1
#=GS R5UQ53_9PORP/12-135      AC R5UQ53.1
#=GS B3EB37_GEOLS/3-105       AC B3EB37.1
#=GS F2NVD3_TRES6/12-144      AC F2NVD3.1
#=GS C4ZAA8_EUBR3/13-138      AC C4ZAA8.1
#=GS R7B2S4_9CLOT/15-146      AC R7B2S4.1
#=GS R5GVJ7_9BACT/12-144      AC R5GVJ7.1
#=GS C5BFF1_EDWI9/13-166      AC C5BFF1.2
#=GS A8G987_SERP5/12-169      AC A8G987.1
#=GS Q9JZ51_NEIMB/26-167      AC Q9JZ51.1
#=GS H2ISN5_RAHAC/12-169      AC H2ISN5.1
#=GS A8AM36_CITK8/13-166      AC A8AM36.1
#=GS A5FU96_ACICJ/14-166      AC A5FU96.1
#=GS D5QBY0_KOMHA/6-115       AC D5QBY0.1
#=GS R5UXG0_9PORP/13-149      AC R5UXG0.1
#=GS E7GQT1_CLOSY/14-144      AC E7GQT1.1
#=GS R6ETE5_9FIRM/14-148      AC R6ETE5.1
#=GS A0A0A0Z842_9ENTR/12-165  AC A0A0A0Z842.1
J9ZE30_LEPFM/14-167                 .....s-RRETLWFAKRLSANDTLANNAHQAGPYIPKNIL.FTLFPSLDRPEDKN..PEVRFRLQIDSHQGERREIRAVWYNNRRRG...KTRDEARITGFGGRSSPFLDPENTGALMLLVVPPGDN..TQTSICRAWVCRNPSEEDLLEDLIGP..VEPGKGFLWAP--ev..............................
R5UQ53_9PORP/12-135                 .vdkvq-----AAFCKFIYANDVGATGAHQDGIYIPKNCW.SLIFDNPSE-KGVN..KDHFVKIRWKNNFETD--NRFIYYGK----...GTRNEYRLTRFGKRFPFFQE-DNVGDLIIICRTE---..--------------------------..-------------ldyyfgfvlsadedidnffg............
B3EB37_GEOLS/3-105                  .ysaqi--------TKILSANDVGETGGHQAGILVPKDLEiLSFFPSLNS-KEKN..PRVTLVFREGDG-ITRWDFNFIYYNNRFFG...GTRNEYRLTCM--------------------------..--------------------------..-------------tqylrarnakvgdhvvls..............
F2NVD3_TRES6/12-144                 ...tan---SERIFCKFLSANDTGETGGHQSGIYIPKNSV.PLIFDTPG-VKGEN..KEEFNKIKWQDDFETDAHFK--YYGQ----...GTRNEYRITGFGRNF-PFLKPAFTGSLVIILKQKDSS..-----YKGYVL---------------..-------------etedeieyfldyfgitpnet............
C4ZAA8_EUBR3/13-138                 ..tvas----ELAFCKFLSANDTGATGGHQGGVLVSVSAS.RMLFVEILP-DNDI..LKRDVKITWQGDLVTESTFT--YYSS----...--KKELRITKFGRDFDII-NPDRTGSLFVLTKQSWDD..-----YSVFIMD--------------..-------------tedeieeflstfgi..................
R7B2S4_9CLOT/15-146                 nsekgl--------CKFLSANDTGATGGHQSGILISKSAV.EMMFSKQELEQAGI..PKRTVKVRWQDDFETESCFT--YYES----...--KNELRITRFGRGF-PFLKPEQTGALFVFTQQTDFD..----YCGYF-----------------..-------------letdeeieefldtfgisptetns.........
R5GVJ7_9BACT/12-144                 .vrsar-----LSFCRFITANDTGDTGAHQAGFYIPKCAA.RLLFETPGQ-KGEN..KSRTVHICWQNDFTTESR--FIYYGR----...GTRNEYRITRFGRNFPFFQE-SATGDLLIVAQESEDD..-----YAAFVL--SSDQDI-EDFLA-..-------------hfnldigsl.......................
C5BFF1_EDWI9/13-166                 ....tg---NAYVYIKRLSANDTGATGAHQVGTYMQHSAA.EYLFPSINKTDECN..PSLMITARTSSHSYPENQVRVIYYNNKFFG...KTRNERRITRWGGG-SPLQDTENTGALALLVFDSSLGgkSDCQSVDVWVCRDADEEDIVEASIGE..ILPGDLIFGLADE................................
A8G987_SERP5/12-169                 .....s-SDNWYIHIKRLSANDTGASGGHQSGVYLPNNSA.NFLFPSINRIDEEN..PSVGLTAKIISHEVNDSQVRAVYYNSKLRNlnpKGRNETRITCWGGQKSPVQNQNNTGAIAIFAFYTPEN.lKDCGLVEAWVCCTVEEEDLLEAIIGE..VLPGTARSGPSA-q...............................
Q9JZ51_NEIMB/26-167                 ......ANKSIAVYCRYIRPNDVGTTGSHQSGFYIQKHFS.DDLFDVVCQ-KGTN..KTISIKINWQDGSVTNSNFK--YYGQ----...GTRNEARITGFGKNFEFLSD-KYSGSLLVLCRACYKD..---LLFHAFVL--SSDEDI-EIFIAEtnILPGSLYLPKKQE................................
H2ISN5_RAHAC/12-169                 ......GNPEWVIYMKRLSANDTGATGGHQVGIYIPKITR.DTLFPSINRVDCDN..PSIPLSAKIVSHDIEEQTVNAIYYNSKLAKqqkKGRNESRITSWGGKKSPIQDPENTGSLLILAFKKQTE.lADCMYLEAWICRSIEEEDLLETITGE..ILPGISVFN----sgds............................
A8AM36_CITK8/13-166                 .....s-TSDTYMFIKRLSANDTGATGGHQVGIYIPSGIV.EHLFPSINHTRDLN..PSVMLNAHTSSHNCPDSEARAIYYNGRFFG...KTRNEKRITRWGGGKNPLQDPENTGALALLAFDHHQG..SDSQFVDIWVCHNAEEEDIVESSLGE..IVPGSLVYGPANE................................
A5FU96_ACICJ/14-166                 ....gv--PGAVWFAKRLAANDTLATGAHGAGPYIPKEFL.FSVFPDLNHPEEEN..PDCWFDLYIDSHA-DRRRIRAIWYNNRLRG...GTRNEARLTNFGGGRSALLDPDSTGALAVFAFLPHGP..GRQMECHVWVCDQGAEADLLEERIGP..VEPKAPVVWQPG-e...............................
D5QBY0_KOMHA/6-115                  .fssai--------FKILSKNDSGMGAGHQAGFLIPTELR.-SFFPPLPEATIENpaPSILIDAILISDQTQPVRVITRWQAQTWYN...QRSPETRLTG---------------------------..--------------------------..-------------eleiirkyscegdllliekseedi........
R5UXG0_9PORP/13-149                 ...kra----KIIFLKLITANDTGATGAHQAGFHLPKNSW.RLFFDSPG-IKGYN..KDKYITIRWGSFS---TESRAIYYGK----...GTRNEYRLTRFGLHFPYLQE-QNIGDILIIIRNFDDT..--------------------------..-------------yegfiifkenilsfwmesglsennlntitina
E7GQT1_CLOSY/14-144                 ...lns----IQGFCKFLSANDTGATGGHQSGILISKSAV.EMLFTKQQLDQEGI..PKRTVKIEWQDDLVTESRFT--YYES----...--KNELRVTRFGRGF-PFLHPDQTGALFVFTKQSEDD..Y-------------------------..-------------sgyfleteeeieeflnafgisptet.......
R6ETE5_9FIRM/14-148                 ..lnsr-----KAYCKFLSANDTGLTGGHQAGIYIAKPSV.PILFDEPG-IKGEN..KEKWVKVKWQDDI--ETDTRFIYYGQ----...GTRNEYRITNFGRGFPF-LRPEYTGALFVFAKCS-DE..----YYQAYILN--SEDDI-------..-------------dqfldafgigptetnrl...............
A0A0A0Z842_9ENTR/12-165             ....ac--ENYFVYIKRLSANDTGATGGHQVGLYIPSGIV.EKLFPSINHTRELN..PSVFLTAHVSSHDCPDSEARAIYYNSRHFG...KTRNEKRITRWGRG-SPLQDPENTGALTLLAFKLDEQ.gGDCKEVNIWVCASTDEEDVIETAIGE..VIPGALISGPA--gq..............................
#=GC seq_cons                       ....st.....hhasKhLSANDTGATGGHQuGlYIPKsss.phLFsslsp.cspN..Pshhlcl+hpsc.hs-sch+hlYYss.......tTRNEhRITpFGtshs.h.cs-pTGuLhllshpptps.......hphalh..............................p...............................
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